Restriction map for pF1KB6783
 (Linear) (Six-base) MAPSORT of: KIBB6783.seq  Check: 7943  from: 1  to: 363



KIBB6783.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

BmtI G_CTAG'C

Cuts at:      0      52     363 
   Size:         52     311
 
BsaBI GATnn'nnATC

Cuts at:      0     272     363 
   Size:        272      91
 
BsaWI w'CCGG_w

Cuts at:      0     109     363 
   Size:        109     254
 
BsrFI r'CCGG_y

Cuts at:      0      40     363 
   Size:         40     323
 
BsrGI T'GTAC_A

Cuts at:      0     241     363 
   Size:        241     122
 
DraI TTT'AAA

Cuts at:      0     261     363 
   Size:        261     102
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      70     363 
   Size:         70     293
 
EarI CTCTTCn'nnn_

Cuts at:      0     207     363 
   Size:        207     156
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     189     363 
   Size:        189     174
 
NaeI GCC'GGC

Cuts at:      0      42     363 
   Size:         42     321
 
NgoMIV G'CCGG_C

Cuts at:      0      40     363 
   Size:         40     323
 
NheI G'CTAG_C

Cuts at:      0      48     363 
   Size:         48     315
 
NspI r_CATG'y

Cuts at:      0      48     363 
   Size:         48     315
 
SphI G_CATG'C

Cuts at:      0      48     363 
   Size:         48     315
 
StyI C'CwwG_G

Cuts at:      0     224     363 
   Size:        224     139
 
TatI w'GTAC_w

Cuts at:      0     241     363 
   Size:        241     122
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     162     363 
   Size:        162     201
 
XmnI GAAnn'nnTTC

Cuts at:      0      98     363 
   Size:         98     265
 

 Enzymes that do cut:

     BmtI    BsaBI    BsaWI    BsrFI    BsrGI     DraI     DrdI     EarI
     MmeI     NaeI   NgoMIV     NheI     NspI     SphI     StyI     TatI
     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI   DraIII     EaeI     EagI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MscI     MslI   MspA1I     NarI     NcoI
     NdeI     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SrfI
     SspI     StuI     SwaI    TaqII     TstI  Tth111I     XbaI     XhoI
     XmaI     ZraI
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