Restriction map for pF1KB7680
##################################
# NO CUT ENZYMES (  122 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AflII       AflIII      AgeI        AhdI        
Alw44I      Aor51HI     ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AvrII       BalI        BbrPI       BbvCI       BciVI       
BclI        BfrI        BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaI        BseAI       BsiWI       
BsmAI       BsmBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrGI       BssHII      Bst1107I    
BstBI       BstUI       BstZ17I     CelII       ClaI        
CpoI        DraI        DraIII      Eam1105I    EarI        
Eco47III    EcoNI       EcoRI       EcoRV       EcoT22I     
FbaI        FseI        HincII      HindII      HindIII     
HpaI        KpnI        Ksp632I     KspI        MamI        
MfeI        MluI        MluNI       MroI        MscI        
MseI        MspA1I      MunI        MvnI        NcoI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsrII       SacII       SalI        SapI        SbfI        
SexAI       SfcI        SfiI        SfuI        SnaBI       
SpeI        Sse8387I    SspBI       SspI        StuI        
SwaI        Tru9I       Tth111I     Van91I      XbaI        
XcmI        XmnI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AcsI         1159  AAATTC               [GA]^AATT[CT]
AocI         1033  CCTGAGG              CC^T[ACGT]AGG
ApoI         1159  AAATTC               [GA]^AATT[CT]
AviII         902  TGCGCA               TGC^GCA
BamHI        1209  GGATCC               G^GATCC
BbeI          964  GGCGCC               GGCGC^C
BbvI           60  GCTGC                GCAGC(8/12)
BcgI          905  GCATTGCCCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI          335  CTAG                 C^TAG
BglII         326  AGATCT               A^GATCT
BsiEI         472  CGGCCG               CG[GA][CT]^CG
BspMI         256  ACCTGC               ACCTGC(4/8)
BsrDI         906  CATTGC               GCAATG(2/0)
BstAPI        441  GCAGGCCATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        697  GGTGACC              G^GT[ACGT]ACC
BstPI         697  GGTGACC              G^GT[ACGT]ACC
BstXI         887  CCAAGTACTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       1033  CCTGAGG              CC^T[ACGT]AGG
DrdI          211  GACTGCATTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          609  CCGTGG               C^C[GA][CT]GG
EagI          472  CGGCCG               C^GGCCG
EclXI         472  CGGCCG               C^GGCCG
Eco52I        472  CGGCCG               C^GGCCG
Eco81I       1033  CCTGAGG              CC^T[ACGT]AGG
EcoO65I       697  GGTGACC              G^GT[ACGT]ACC
FspI          902  TGCGCA               TGC^GCA
HaeII         964  GGCGCC               [GA]GCGC^[CT]
KasI          964  GGCGCC               G^GCGCC
MaeI          335  CTAG                 C^TAG
MslI          498  CACAACGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI            9  GCCGGC               GCC^GGC
NarI          964  GGCGCC               GG^CGCC
NgoMIV          9  GCCGGC               G^CCGGC
PaeR7I        913  CTCGAG               C^TCGAG
PpuMI         981  AGGACCT              [GA]G^G[AT]CC[CT]
ScaI          890  AGTACT               AGT^ACT
SfaNI         310  GATGC                GCATC(5/9)
SfoI          964  GGCGCC               GGC^GCC
SgrAI           8  CGCCGGCG             C[GA]^CCGG[CT]G
SmaI          526  CCCGGG               CCC^GGG
SmlI          913  CTCGAG               C^T[CT][GA]AG
SphI          437  GCATGC               GCATG^C
TaqI          914  TCGA                 T^CGA
TfiI          200  GATTC                G^A[AT]TC
TseI           60  GCTGC                G^C[AT]GC
TthHB8I       914  TCGA                 T^CGA
XhoI          913  CTCGAG               C^TCGAG
XmaCI         526  CCCGGG               C^CCGGG
XmaI          526  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AfaI          891  GTAC                 GT^AC
             1080  GTAC                
AlwNI         198  CAGATTCTG            CAG[ACGT][ACGT][ACGT]^CTG
              587  CAGGCCCTG           
AvaI          526  CCCGGG               C^[CT]CG[GA]G
              913  CTCGAG              
BbsI          688  GAAGAC               GAAGAC(2/6)
              852  GAAGAC              
BglI          945  GCCAGGAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1055  GCCTGGAGGGC         
BpuAI         688  GAAGAC               GAAGAC(2/6)
              852  GAAGAC              
BsaWI          65  ACCGGA               [AT]^CCGG[AT]
              976  ACCGGA              
BsgI           61  CTGCAC               GTGCAG(16/14)
              391  CTGCAC              
BsoBI         526  CCCGGG               C^[CT]CG[GA]G
              913  CTCGAG              
BsrBI         234  GAGCGG               CCGCTC(-3/-3)
              916  GAGCGG              
BsrFI           9  GCCGGC               [GA]^CCGG[CT]
              679  GCCGGT              
BssSI         790  CTCGTG               CACGAG(-5/-1)
              814  CTCGTG              
BstF5I         90  GGATG                GGATG(2/0)
              543  GGATG               
BstYI         326  AGATCT               [GA]^GATC[CT]
             1209  GGATCC              
Cfr10I          9  GCCGGC               [GA]^CCGG[CT]
              679  GCCGGT              
EcoT14I       182  CCAAGG               C^C[AT][AT]GG
             1205  CCAAGG              
FokI           90  GGATG                GGATG(9/13)
              543  GGATG               
HgaI            6  GACGC                GACGC(5/10)
             1064  GCGTC               
MaeII          37  ACGT                 A^CGT
             1120  ACGT                
MflI          326  AGATCT               [GA]^GATC[CT]
             1209  GGATCC              
NspI          158  ACATGC               [GA]CATG^[CT]
              437  GCATGC              
RsaI          891  GTAC                 GT^AC
             1080  GTAC                
StyI          182  CCAAGG               C^C[AT][AT]GG
             1205  CCAAGG              
Tsp509I       783  AATT                 ^AATT
             1160  AATT                
XhoII         326  AGATCT               [GA]^GATC[CT]
             1209  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   62 enzymes) #
#########################################

AciI        AcyI        AluI        AlwI        ApaI        
AspHI       AvaII       BanI        BanII       BcnI        
BmyI        BpmI        BsaHI       BsaJI       BseRI       
BsiHKAI     BsiYI       BslI        BsmFI       BsmI        
Bsp1286I    BsrI        BssKI       BstNI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
EaeI        EcoO109I    EcoRII      FauI        Fnu4HI      
HaeIII      HapII       HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        MaeIII      
MboI        MboII       MnlI        MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PleI        SacI        Sau3AI      Sau96I      ScrFI       
Tsp45I      TspRI       
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