Restriction map for pF1KB7680
 (Linear) (Six-base) MAPSORT of: KIBB7680.seq  Check: 8328  from: 1  to: 1218



KIBB7680.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:49  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     184    1218 
   Size:        184    1034
 
AlwNI CAG_nnn'CTG

Cuts at:      0     203     592    1218 
   Size:        203     389     626

  Fragments arranged by size: 

                 626     389     203
 
ApaI G_GGCC'C

Cuts at:      0     459     736     756    1218 
   Size:        459     277      20     462

  Fragments arranged by size: 

                 462     459     277      20
 
ApoI r'AATT_y

Cuts at:      0    1159    1218 
   Size:       1159      59
 
AvaI C'yCGr_G

Cuts at:      0     526     913    1218 
   Size:        526     387     305

  Fragments arranged by size: 

                 526     387     305
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     469     502    1218 
   Size:        469      33     716

  Fragments arranged by size: 

                 716     469      33
 
BamHI G'GATC_C

Cuts at:      0    1209    1218 
   Size:       1209       9
 
BanI G'GyrC_C

Cuts at:      0     676     964    1012    1218 
   Size:        676     288      48     206

  Fragments arranged by size: 

                 676     288     206      48
 
BanII G_rGCy'C

Cuts at:      0     415     459     527     562     736     756     791    1218 
   Size:        415      44      68      35     174      20      35     427

  Fragments arranged by size: 

                 427     415     174      68      44      35      35      20
            
 
BbeI G_GCGC'C

Cuts at:      0     968    1218 
   Size:        968     250
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     695     859    1218 
   Size:        695     164     359

  Fragments arranged by size: 

                 695     359     164
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     894     928    1218 
   Size:        894      34     290

  Fragments arranged by size: 

                 894     290      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     951    1061    1218 
   Size:        951     110     157

  Fragments arranged by size: 

                 951     157     110
 
BglII A'GATC_T

Cuts at:      0     326    1218 
   Size:        326     892
 
Bme1580I G_kGCm'C

Cuts at:      0     145     459     736     756    1218 
   Size:        145     314     277      20     462

  Fragments arranged by size: 

                 462     314     277     145      20
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     869    1218 
   Size:        869     349
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     399     977    1078    1121    1162    1204    1218 
   Size:        399     578     101      43      41      42      14

  Fragments arranged by size: 

                 578     399     101      43      42      41      14
 
Bpu10I CC'TnA_GC

Cuts at:      0     382    1218 
   Size:        382     836
 
BsaHI Gr'CG_yC

Cuts at:      0       7     965    1064    1218 
   Size:          7     958      99     154

  Fragments arranged by size: 

                 958     154      99       7
 
BsaWI w'CCGG_w

Cuts at:      0      65     976    1218 
   Size:         65     911     242

  Fragments arranged by size: 

                 911     242      65
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     539     569     868     898    1218 
   Size:        539      30     299      30     320

  Fragments arranged by size: 

                 539     320     299      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     439    1034    1051    1218 
   Size:        439     595      17     167

  Fragments arranged by size: 

                 595     439     167      17
 
BseYI C'CCAG_C

Cuts at:      0     458     729     757    1051    1218 
   Size:        458     271      28     294     167

  Fragments arranged by size: 

                 458     294     271     167      28
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      46     376    1218 
   Size:         46     330     842

  Fragments arranged by size: 

                 842     330      46
 
BsiEI CG_ry'CG

Cuts at:      0     475    1218 
   Size:        475     743
 
BsiHKAI G_wGCw'C

Cuts at:      0     415     562     791    1218 
   Size:        415     147     229     427

  Fragments arranged by size: 

                 427     415     229     147
 
BsmI GAATG_Cn'

Cuts at:      0      21      30     126     870    1218 
   Size:         21       9      96     744     348

  Fragments arranged by size: 

                 744     348      96      21       9
 
Bsp1286I G_dGCh'C

Cuts at:      0     145     415     459     527     562     736     756     791 
   Size:        145     270      44      68      35     174      20      35

Cuts at:    791    1218 
   Size:        427

  Fragments arranged by size: 

                 427     270     174     145      68      44      35      35
                  20
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     265    1218 
   Size:        265     953
 
BsrBI CCG'CTC

Cuts at:      0     236     918    1218 
   Size:        236     682     300

  Fragments arranged by size: 

                 682     300     236
 
BsrDI GCAATG_nn'

Cuts at:      0     905    1218 
   Size:        905     313
 
BsrFI r'CCGG_y

Cuts at:      0       9     679    1218 
   Size:          9     670     539

  Fragments arranged by size: 

                 670     539       9
 
BssSI C'ACGA_G

Cuts at:      0     790     814    1218 
   Size:        790      24     404

  Fragments arranged by size: 

                 790     404      24
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     447    1218 
   Size:        447     771
 
BstEII G'GTnAC_C

Cuts at:      0     697    1218 
   Size:        697     521
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     894    1218 
   Size:        894     324
 
BstYI r'GATC_y

Cuts at:      0     326    1209    1218 
   Size:        326     883       9

  Fragments arranged by size: 

                 883     326       9
 
Bsu36I CC'TnA_GG

Cuts at:      0    1034    1218 
   Size:       1034     184
 
BtgI C'CryG_G

Cuts at:      0     609    1218 
   Size:        609     609
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1077    1218 
   Size:       1077     141
 
BtsI GCAGTG_nn'

Cuts at:      0     121     639    1218 
   Size:        121     518     579

  Fragments arranged by size: 

                 579     518     121
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     217    1218 
   Size:        217    1001
 
EaeI y'GGCC_r

Cuts at:      0     292     472    1177    1218 
   Size:        292     180     705      41

  Fragments arranged by size: 

                 705     292     180      41
 
EagI C'GGCC_G

Cuts at:      0     472    1218 
   Size:        472     746
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     367     558    1218 
   Size:        367     191     660

  Fragments arranged by size: 

                 660     367     191
 
EcoICRI GAG'CTC

Cuts at:      0     413     560     789    1218 
   Size:        413     147     229     429

  Fragments arranged by size: 

                 429     413     229     147
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     184     399     977    1078    1121    1162    1204    1218 
   Size:        184     215     578     101      43      41      42      14

  Fragments arranged by size: 

                 578     215     184     101      43      42      41      14
            
 
EcoO109I rG'GnC_Cy

Cuts at:      0     455     589     733     752     753     952     982    1218 
   Size:        455     134     144      19       1     199      30     236

  Fragments arranged by size: 

                 455     236     199     144     134      30      19       1
            
 
FspI TGC'GCA

Cuts at:      0     904    1218 
   Size:        904     314
 
HaeII r_GCGC'y

Cuts at:      0     968    1218 
   Size:        968     250
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     359     391     544     576     868     900    1218 
   Size:        359      32     153      32     292      32     318

  Fragments arranged by size: 

                 359     318     292     153      32      32      32
 
KasI G'GCGC_C

Cuts at:      0     964    1218 
   Size:        964     254
 
MslI CAynn'nnrTG

Cuts at:      0     502    1218 
   Size:        502     716
 
NaeI GCC'GGC

Cuts at:      0      11    1218 
   Size:         11    1207
 
NarI GG'CG_CC

Cuts at:      0     965    1218 
   Size:        965     253
 
NgoMIV G'CCGG_C

Cuts at:      0       9    1218 
   Size:          9    1209
 
NspI r_CATG'y

Cuts at:      0     162     441    1218 
   Size:        162     279     777

  Fragments arranged by size: 

                 777     279     162
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     778     810    1218 
   Size:        778      32     408

  Fragments arranged by size: 

                 778     408      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     982    1218 
   Size:        982     236
 
PspOMI G'GGCC_C

Cuts at:      0     455     732     752    1218 
   Size:        455     277      20     466

  Fragments arranged by size: 

                 466     455     277      20
 
PspXI vC'TCGA_Gb

Cuts at:      0     913    1218 
   Size:        913     305
 
SacI G_AGCT'C

Cuts at:      0     415     562     791    1218 
   Size:        415     147     229     427

  Fragments arranged by size: 

                 427     415     229     147
 
ScaI AGT'ACT

Cuts at:      0     892    1218 
   Size:        892     326
 
SfoI GGC'GCC

Cuts at:      0     966    1218 
   Size:        966     252
 
SgrAI Cr'CCGG_yG

Cuts at:      0       9    1218 
   Size:          9    1209
 
SmaI CCC'GGG

Cuts at:      0     528    1218 
   Size:        528     690
 
SmlI C'TyrA_G

Cuts at:      0     913    1218 
   Size:        913     305
 
SphI G_CATG'C

Cuts at:      0     441    1218 
   Size:        441     777
 
StyI C'CwwG_G

Cuts at:      0     182    1205    1218 
   Size:        182    1023      13

  Fragments arranged by size: 

                1023     182      13
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     107     380     658     980    1218 
   Size:        107     273     278     322     238

  Fragments arranged by size: 

                 322     278     273     238     107
 
TatI w'GTAC_w

Cuts at:      0     890    1079    1218 
   Size:        890     189     139

  Fragments arranged by size: 

                 890     189     139
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     539     571    1194    1218 
   Size:        539      32     623      24

  Fragments arranged by size: 

                 623     539      32      24
 
XhoI C'TCGA_G

Cuts at:      0     913    1218 
   Size:        913     305
 
XmaI C'CCGG_G

Cuts at:      0     526    1218 
   Size:        526     692
 

 Enzymes that do cut:

     AcuI    AlwNI     ApaI     ApoI     AvaI     BaeI    BamHI     BanI
    BanII     BbeI     BbsI     BcgI     BglI    BglII Bme1580I     BmrI
     BpmI   Bpu10I    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI     BsmI Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI
    BssSI   BstAPI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI
     BtsI     DrdI     EaeI     EagI     EciI  EcoICRI  Eco57MI EcoO109I
     FspI    HaeII    Hin4I     KasI     MslI     NaeI     NarI   NgoMIV
     NspI     PpiI    PpuMI   PspOMI    PspXI     SacI     ScaI     SfoI
    SgrAI     SmaI     SmlI     SphI     StyI    TaqII     TatI     TstI
     XhoI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    ApaLI     AscI     AseI
    AsiSI    AvrII    BbvCI    BciVI     BclI    BfrBI     BlpI    BmgBI
     BmtI     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsiWI    BsmBI
    BspEI    BspHI    BsrGI   BssHII    BstBI  BstZ17I     ClaI    CspCI
     DraI   DraIII     EarI    EcoNI    EcoRI    EcoRV     FalI     FseI
    FspAI   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI
     MscI   MspA1I     NcoI     NdeI     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI    PshAI
     PsiI     PsrI     PstI     PvuI    PvuII    RsrII    SacII     SalI
    SanDI     SapI     SbfI    SexAI     SfcI     SfiI     SgfI    SnaBI
     SpeI     SrfI     SspI     StuI     SwaI  Tth111I     XbaI     XcmI
     XmnI     ZraI
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