Restriction map for pF1KB8913
##################################
# NO CUT ENZYMES (  149 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwI        AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AviII       AvrII       
BalI        BamHI       BbeI        BbrPI       BcgI        
BciVI       BfaI        BfrI        BglII       BlnI        
BlpI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaWI       BseAI       BsiWI       BsmBI       BsmFI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrDI       BsrGI       BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstXI       BstYI       
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
DraI        DraII       DraIII      Eam1105I    Eco47III    
Eco81I      EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT22I     FseI        FspI        HgaI        Hin1I       
HincII      HindII      HindIII     HinfI       HpaI        
KasI        KpnI        KspI        MaeI        MaeII       
MamI        MflI        MluI        MluNI       MroI        
MscI        MseI        MslI        NarI        NdeI        
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SbfI        ScaI        SexAI       SfaNI       SfcI        
SfiI        SfoI        SfuI        SgrAI       SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
SwaI        TfiI        Tru9I       Tth111I     Van91I      
XbaI        XcmI        XhoI        XhoII       


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AccI          592  GTAGAC               GT^[AC][GT]AC
AfaI          626  GTAC                 GT^AC
AlwNI         356  CAGTGACTG            CAG[ACGT][ACGT][ACGT]^CTG
AspHI         538  GTGCTC               G[AT]GC[AT]^C
AvaII         746  GGTCC                G^G[AT]CC
BanII          40  GAGCCC               G[GA]GC[CT]^C
BbsI          466  GAAGAC               GAAGAC(2/6)
BbvCI          55  GCTGAGG              CCTCAGC(-5/-2)
BclI          173  TGATCA               T^GATCA
BglI           46  GCCCCGGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI         466  GAAGAC               GAAGAC(2/6)
BsaI          412  GGTCTC               GGTCTC(1/5)
BsgI          244  GTGCAG               GTGCAG(16/14)
BsiEI          26  CGGCCG               CG[GA][CT]^CG
BsiHKAI       538  GTGCTC               G[AT]GC[AT]^C
BsmI          455  GAATGC               GAATGC(1/-1)
BspMI         441  GCAGGT               ACCTGC(4/8)
BssHII        133  GCGCGC               G^CGCGC
BstF5I        631  CATCC                GGATG(2/0)
DrdI          595  GACCTCTATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI           26  CGGCCG               C^GGCCG
EarI          666  GAAGAG               CTCTTC(1/4)
EclXI          26  CGGCCG               C^GGCCG
Eco52I         26  CGGCCG               C^GGCCG
EcoNI         606  CCTGGAGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI           43  CCCGC                CCCGC(4/6)
FbaI          173  TGATCA               T^GATCA
FokI          631  CATCC                GGATG(9/13)
Ksp632I       666  GAAGAG               CTCTTC(1/4)
MaeIII        358  GTGAC                ^GT[ACGT]AC
MfeI          434  CAATTG               C^AATTG
MunI          434  CAATTG               C^AATTG
NaeI           24  GCCGGC               GCC^GGC
NcoI          749  CCATGG               C^CATGG
NgoMIV         24  GCCGGC               G^CCGGC
NspI          500  ACATGC               [GA]CATG^[CT]
PvuII         526  CAGCTG               CAG^CTG
RsaI          626  GTAC                 GT^AC
SapI          666  GAAGAGC              GCTCTTC(1/4)
SmlI           96  CTTGAG               C^T[CT][GA]AG
StuI          614  AGGCCT               AGG^CCT
Tsp45I        358  GTGAC                ^GT[GC]AC


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

Asp700I       612  GAAGGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              655  GAAGCCTTTC          
AvaI           74  CCCGGG               C^[CT]CG[GA]G
              254  CCCGGG              
BanI          124  GGTGCC               G^G[CT][GA]CC
              203  GGCACC              
BbvI           70  GCAGC                GCAGC(8/12)
               86  GCAGC               
BmyI           40  GAGCCC               G[AGT]GC[ACT]^C
              538  GTGCTC              
BsmAI         391  GAGAC                GTCTC(1/5)
              413  GTCTC               
BsoBI          74  CCCGGG               C^[CT]CG[GA]G
              254  CCCGGG              
Bsp1286I       40  GAGCCC               G[AGT]GC[ACT]^C
              538  GTGCTC              
BsrBI          79  GAGCGG               CCGCTC(-3/-3)
               99  GAGCGG              
BsrFI          24  GCCGGC               [GA]^CCGG[CT]
              200  ACCGGC              
BsrI          295  CCAGT                ACTGG(1/-1)
              700  CCAGT               
Cfr10I         24  GCCGGC               [GA]^CCGG[CT]
              200  ACCGGC              
DpnII         174  GATC                 ^GATC
              484  GATC                
DsaI          144  CCGTGG               C^C[GA][CT]GG
              749  CCATGG              
EcoT14I       191  CCAAGG               C^C[AT][AT]GG
              749  CCATGG              
HaeII         210  GGCGCT               [GA]GCGC^[CT]
              258  GGCGCT              
MboI          174  GATC                 ^GATC
              484  GATC                
MspA1I        267  CCGCTG               C[AC]G^C[GT]G
              526  CAGCTG              
NdeII         174  GATC                 ^GATC
              484  GATC                
Sau3AI        174  GATC                 ^GATC
              484  GATC                
SmaI           74  CCCGGG               CCC^GGG
              254  CCCGGG              
StyI          191  CCAAGG               C^C[AT][AT]GG
              749  CCATGG              
TaqI          366  TCGA                 T^CGA
              453  TCGA                
TseI           70  GCAGC                G^C[AT]GC
               86  GCAGC               
TthHB8I       366  TCGA                 T^CGA
              453  TCGA                
XmaCI          74  CCCGGG               C^CCGGG
              254  CCCGGG              
XmaI           74  CCCGGG               C^CCGGG
              254  CCCGGG              
XmnI          612  GAAGGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              655  GAAGCCTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   39 enzymes) #
#########################################

AccII       AciI        AluI        BcnI        BpmI        
BsaJI       BseRI       BsiYI       BslI        BssKI       
BstNI       BstUI       Cac8I       CfoI        Cfr13I      
DdeI        EaeI        EcoRII      Fnu4HI      HaeIII      
HapII       HhaI        HinP1I      HpaII       HphI        
ItaI        MboII       MnlI        MspI        MvaI        
MvnI        MwoI        NciI        NlaIII      NlaIV       
Sau96I      ScrFI       Tsp509I     TspRI       
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