Restriction map for pF1KB8913
 (Linear) (Six-base) MAPSORT of: KIBB8913.seq  Check: 8821  from: 1  to: 759



KIBB8913.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:30  ..

AccI GT'mk_AC

Cuts at:      0     593     759 
   Size:        593     166
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     685     759 
   Size:        685      74
 
AlwNI CAG_nnn'CTG

Cuts at:      0     361     759 
   Size:        361     398
 
AvaI C'yCGr_G

Cuts at:      0      74     254     759 
   Size:         74     180     505

  Fragments arranged by size: 

                 505     180      74
 
BanI G'GyrC_C

Cuts at:      0     124     203     759 
   Size:        124      79     556

  Fragments arranged by size: 

                 556     124      79
 
BanII G_rGCy'C

Cuts at:      0      44     759 
   Size:         44     715
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     473     759 
   Size:        473     286
 
BbvCI CC'TCA_GC

Cuts at:      0      56     759 
   Size:         56     703
 
BclI T'GATC_A

Cuts at:      0     173     759 
   Size:        173     586
 
BglI GCCn_nnn'nGGC

Cuts at:      0      52     759 
   Size:         52     707
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     289     759 
   Size:        289     470
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     613     759 
   Size:        613     146
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     127     235     628     759 
   Size:        127     108     393     131

  Fragments arranged by size: 

                 393     131     127     108
 
Bpu10I CC'TnA_GC

Cuts at:      0      56     759 
   Size:         56     703
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     117     759 
   Size:        117     642
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     418     759 
   Size:        418     341
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     664     694     759 
   Size:        664      30      65

  Fragments arranged by size: 

                 664      65      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      52     253     672     759 
   Size:         52     201     419      87

  Fragments arranged by size: 

                 419     201      87      52
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     265     759 
   Size:        265     494
 
BsiEI CG_ry'CG

Cuts at:      0      29     759 
   Size:         29     730
 
BsiHKAI G_wGCw'C

Cuts at:      0     542     759 
   Size:        542     217
 
BsmI GAATG_Cn'

Cuts at:      0     461     759 
   Size:        461     298
 
Bsp1286I G_dGCh'C

Cuts at:      0      44     542     759 
   Size:         44     498     217

  Fragments arranged by size: 

                 498     217      44
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     432     759 
   Size:        432     327
 
BsrBI CCG'CTC

Cuts at:      0      81     101     759 
   Size:         81      20     658

  Fragments arranged by size: 

                 658      81      20
 
BsrFI r'CCGG_y

Cuts at:      0      24     200     759 
   Size:         24     176     559

  Fragments arranged by size: 

                 559     176      24
 
BssHII G'CGCG_C

Cuts at:      0     133     759 
   Size:        133     626
 
BtgI C'CryG_G

Cuts at:      0     144     749     759 
   Size:        144     605      10

  Fragments arranged by size: 

                 605     144      10
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     601     759 
   Size:        601     158
 
EaeI y'GGCC_r

Cuts at:      0       8      26      65     147     759 
   Size:          8      18      39      82     612

  Fragments arranged by size: 

                 612      82      39      18       8
 
EagI C'GGCC_G

Cuts at:      0      26     759 
   Size:         26     733
 
EarI CTCTTCn'nnn_

Cuts at:      0     661     759 
   Size:        661      98
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     252     759 
   Size:        252     507
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     127     235     628     685     759 
   Size:        127     108     393      57      74

  Fragments arranged by size: 

                 393     127     108      74      57
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     610     759 
   Size:        610     149
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     201     759 
   Size:        201     558
 
HaeII r_GCGC'y

Cuts at:      0     214     262     759 
   Size:        214      48     497

  Fragments arranged by size: 

                 497     214      48
 
MfeI C'AATT_G

Cuts at:      0     434     759 
   Size:        434     325
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     347     443     521     759 
   Size:        347      96      78     238

  Fragments arranged by size: 

                 347     238      96      78
 
MspA1I CmG'CkG

Cuts at:      0     269     528     759 
   Size:        269     259     231

  Fragments arranged by size: 

                 269     259     231
 
NaeI GCC'GGC

Cuts at:      0      26     759 
   Size:         26     733
 
NcoI C'CATG_G

Cuts at:      0     749     759 
   Size:        749      10
 
NgoMIV G'CCGG_C

Cuts at:      0      24     759 
   Size:         24     735
 
NspI r_CATG'y

Cuts at:      0     504     759 
   Size:        504     255
 
PasI CC'CwG_GG

Cuts at:      0     408     759 
   Size:        408     351
 
PfoI T'CCnGG_A

Cuts at:      0     605     759 
   Size:        605     154
 
PvuII CAG'CTG

Cuts at:      0     528     759 
   Size:        528     231
 
SapI GCTCTTCn'nnn_

Cuts at:      0     661     759 
   Size:        661      98
 
SmaI CCC'GGG

Cuts at:      0      76     256     759 
   Size:         76     180     503

  Fragments arranged by size: 

                 503     180      76
 
SmlI C'TyrA_G

Cuts at:      0      96     759 
   Size:         96     663
 
SrfI GCCC'GGGC

Cuts at:      0     256     759 
   Size:        256     503
 
StuI AGG'CCT

Cuts at:      0     616     759 
   Size:        616     143
 
StyI C'CwwG_G

Cuts at:      0     191     749     759 
   Size:        191     558      10

  Fragments arranged by size: 

                 558     191      10
 
XmaI C'CCGG_G

Cuts at:      0      74     254     759 
   Size:         74     180     505

  Fragments arranged by size: 

                 505     180      74
 
XmnI GAAnn'nnTTC

Cuts at:      0     616     659     759 
   Size:        616      43     100

  Fragments arranged by size: 

                 616     100      43
 

 Enzymes that do cut:

     AccI     AcuI    AlwNI     AvaI     BanI    BanII     BbsI    BbvCI
     BclI     BglI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaXI    BseRI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspMI
    BsrBI    BsrFI   BssHII     BtgI     DrdI     EaeI     EagI     EarI
     EciI  Eco57MI    EcoNI     FalI    HaeII     MfeI     MmeI   MspA1I
     NaeI     NcoI   NgoMIV     NspI     PasI     PfoI    PvuII     SapI
     SmaI     SmlI     SrfI     StuI     StyI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI     BcgI    BciVI
    BfrBI    BglII     BlpI Bme1580I    BmgBI     BmtI    BsaAI    BsaBI
    BsaHI    BsaWI    BseYI    BsiWI    BsmBI    BspEI    BspHI    BsrDI
    BsrGI    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII  EcoICRI
 EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MluI     MscI     MslI     NarI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PciI    PflMI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
    SnaBI     SpeI     SphI     SspI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     ZraI
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