Restriction map for pF1KB7844
##################################
# NO CUT ENZYMES (  138 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AflII       AflIII      AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AvaI        
AviII       AvrII       BalI        BamHI       BbrPI       
BbsI        BbvCI       BcgI        BclI        BcnI        
BfaI        BfrI        BglII       BlnI        BlpI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BseAI       BsiEI       BsiWI       BsoBI       BspDI       
BspEI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstXI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DraII       DraIII      DrdI        DsaI        
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoT14I     FauI        FbaI        FseI        
FspI        HincII      HindII      HindIII     HpaI        
KpnI        KspI        MaeI        MamI        MluI        
MluNI       MroI        MscI        MvnI        NaeI        
NciI        NcoI        NgoMIV      NheI        NotI        
NruI        NspV        PacI        PaeR7I      PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        RsrII       SacI        
SacII       SalI        SbfI        SfiI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    StuI        StyI        XbaI        XcmI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AciI          789  GCGG                 CCGC(-3/-1)
AcyI         1543  GGCGCC               G[GA]^CG[CT]C
AlwNI         901  CAGAAACTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI         1078  GGGCCC               GGGCC^C
AvaII        1639  GGACC                G^G[AT]CC
BanII        1078  GGGCCC               G[GA]GC[CT]^C
BbeI         1543  GGCGCC               GGCGC^C
BbvI           98  GCAGC                GCAGC(8/12)
BciVI        1229  GTATCC               GTATCC(6/5)
BglI         1108  GCCTCAATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI        1543  GGCGCC               G[GA]^CG[CT]C
BsaWI           7  TCCGGT               [AT]^CCGG[AT]
BsgI         1610  GTGCAG               GTGCAG(16/14)
BsmBI         254  GAGACG               CGTCTC(1/5)
Bsp1407I      705  TGTACA               T^GTACA
BsrGI         705  TGTACA               T^GTACA
BsrI         1529  CCAGT                ACTGG(1/-1)
BstYI        1594  GGATCT               [GA]^GATC[CT]
CfoI         1544  GCGC                 GCG^C
DraI         1266  TTTAAA               TTT^AAA
EcoRV         833  GATATC               GAT^ATC
EcoT22I      1583  ATGCAT               ATGCA^T
Fnu4HI         98  GCAGC                GC^[ACGT]GC
HaeII        1543  GGCGCC               [GA]GCGC^[CT]
HapII           8  CCGG                 C^CGG
HgaI         1324  GCGTC                GACGC(5/10)
HhaI         1544  GCGC                 GCG^C
Hin1I        1543  GGCGCC               G[GA]^CG[CT]C
HinP1I       1544  GCGC                 G^CGC
HpaII           8  CCGG                 C^CGG
ItaI           98  GCAGC                GC^[ACGT]GC
KasI         1543  GGCGCC               G^GCGCC
MaeII         920  ACGT                 A^CGT
MflI         1594  GGATCT               [GA]^GATC[CT]
MslI          223  CATCCTCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        406  CAGCTG               C[AC]G^C[GT]G
MspI            8  CCGG                 C^CGG
NarI         1543  GGCGCC               GG^CGCC
NdeI         1742  CATATG               CA^TATG
NsiI         1583  ATGCAT               ATGCA^T
NspI         1370  ACATGC               [GA]CATG^[CT]
PvuII         406  CAGCTG               CAG^CTG
SapI          496  GCTCTTC              GCTCTTC(1/4)
ScaI         1651  AGTACT               AGT^ACT
SexAI         945  ACCTGGT              A^CC[AT]GGT
SfoI         1543  GGCGCC               GGC^GCC
SspBI         705  TGTACA               T^GTACA
SspI          609  AATATT               AAT^ATT
SwaI         1265  ATTTAAAT             ATTT^AAAT
TseI           98  GCAGC                G^C[AT]GC
XhoII        1594  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AfaI          706  GTAC                 GT^AC
             1652  GTAC                
Asp700I       607  GAAATATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1747  GAATTTTTTC          
AspHI         112  GAGCAC               G[AT]GC[AT]^C
              536  GTGCTC              
AspI          944  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1306  GACATAGTC           
BanI         1116  GGCACC               G^G[CT][GA]CC
             1543  GGCGCC              
BsiHKAI       112  GAGCAC               G[AT]GC[AT]^C
              536  GTGCTC              
BsmFI        1638  GGGAC                GGGAC(10/14)
              394  GTCCC               
BsmI           59  GAATGC               GAATGC(1/-1)
              101  GCATTC              
BspHI         228  TCATGA               T^CATGA
             1008  TCATGA              
BstF5I        223  CATCC                GGATG(2/0)
             1328  CATCC               
EarI          497  CTCTTC               CTCTTC(1/4)
             1524  CTCTTC              
FokI          223  CATCC                GGATG(9/13)
             1328  CATCC               
HphI          441  GGTGA                GGTGA(8/7)
             1072  TCACC               
Ksp632I       497  CTCTTC               CTCTTC(1/4)
             1524  CTCTTC              
MfeI          962  CAATTG               C^AATTG
             1723  CAATTG              
MunI          962  CAATTG               C^AATTG
             1723  CAATTG              
PflFI         944  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1306  GACATAGTC           
PflMI         306  CCAAAATTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              940  CCAAGACCTGG         
PleI         1732  GACTC                GAGTC(4/5)
             1633  GAGTC               
PstI          249  CTGCAG               CTGCA^G
              548  CTGCAG              
RcaI          228  TCATGA               T^CATGA
             1008  TCATGA              
RsaI          706  GTAC                 GT^AC
             1652  GTAC                
SmlI         1086  CTCAAG               C^T[CT][GA]AG
             1500  CTTGAG              
TaqI          321  TCGA                 T^CGA
              514  TCGA                
Tth111I       944  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1306  GACATAGTC           
TthHB8I       321  TCGA                 T^CGA
              514  TCGA                
Van91I        306  CCAAAATTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              940  CCAAGACCTGG         
XmnI          607  GAAATATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1747  GAATTTTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   41 enzymes) #
#########################################

AcsI        AluI        AlwI        ApoI        BmyI        
BsaI        BsaJI       BseRI       BsiYI       BslI        
BsmAI       Bsp1286I    BssKI       BstNI       Cac8I       
Cfr13I      DdeI        DpnII       EcoRII      HaeIII      
HinfI       MaeIII      MboI        MboII       MnlI        
MseI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        TfiI        Tru9I       Tsp45I      Tsp509I     
TspRI       
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