Restriction map for
pF1KB7844
(Linear) (Six-base) MAPSORT of: KIBB7844.seq Check: 1533 from: 1 to: 1761
KIBB7844.orf
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1587 1761
Size: 1587 174
AlwNI CAG_nnn'CTG
Cuts at: 0 906 1761
Size: 906 855
ApaI G_GGCC'C
Cuts at: 0 1082 1761
Size: 1082 679
ApoI r'AATT_y
Cuts at: 0 274 309 1747 1761
Size: 274 35 1438 14
Fragments arranged by size:
1438 274 35 14
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 63 96 1761
Size: 63 33 1665
Fragments arranged by size:
1665 63 33
BanI G'GyrC_C
Cuts at: 0 1116 1543 1761
Size: 1116 427 218
Fragments arranged by size:
1116 427 218
BanII G_rGCy'C
Cuts at: 0 1082 1761
Size: 1082 679
BbeI G_GCGC'C
Cuts at: 0 1547 1761
Size: 1547 214
BciVI GTATCCnnnnn_n'
Cuts at: 0 1240 1761
Size: 1240 521
BfrBI ATG'CAT
Cuts at: 0 1585 1761
Size: 1585 176
BglI GCCn_nnn'nGGC
Cuts at: 0 1114 1761
Size: 1114 647
Bme1580I G_kGCm'C
Cuts at: 0 1082 1761
Size: 1082 679
Bpu10I CC'TnA_GC
Cuts at: 0 1167 1761
Size: 1167 594
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1071 1521 1761
Size: 1071 450 240
Fragments arranged by size:
1071 450 240
BsaI GGTCTCn'nnnn_
Cuts at: 0 786 930 955 1761
Size: 786 144 25 806
Fragments arranged by size:
806 786 144 25
BsaHI Gr'CG_yC
Cuts at: 0 1544 1761
Size: 1544 217
BsaWI w'CCGG_w
Cuts at: 0 7 1761
Size: 7 1754
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 935 965 1761
Size: 935 30 796
Fragments arranged by size:
935 796 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 611 614 943 1761
Size: 611 3 329 818
Fragments arranged by size:
818 611 329 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1631 1761
Size: 1631 130
BsiHKAI G_wGCw'C
Cuts at: 0 116 540 1761
Size: 116 424 1221
Fragments arranged by size:
1221 424 116
BsmI GAATG_Cn'
Cuts at: 0 65 101 1761
Size: 65 36 1660
Fragments arranged by size:
1660 65 36
BsmBI CGTCTCn'nnnn_
Cuts at: 0 248 1761
Size: 248 1513
Bsp1286I G_dGCh'C
Cuts at: 0 116 540 1082 1761
Size: 116 424 542 679
Fragments arranged by size:
679 542 424 116
BspHI T'CATG_A
Cuts at: 0 228 1008 1761
Size: 228 780 753
Fragments arranged by size:
780 753 228
BsrGI T'GTAC_A
Cuts at: 0 705 1761
Size: 705 1056
BstYI r'GATC_y
Cuts at: 0 1594 1761
Size: 1594 167
BtsI GCAGTG_nn'
Cuts at: 0 1416 1761
Size: 1416 345
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 564 599 1761
Size: 564 35 1162
Fragments arranged by size:
1162 564 35
DraI TTT'AAA
Cuts at: 0 1268 1761
Size: 1268 493
EarI CTCTTCn'nnn_
Cuts at: 0 503 1530 1761
Size: 503 1027 231
Fragments arranged by size:
1027 503 231
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1587 1761
Size: 1587 174
EcoRV GAT'ATC
Cuts at: 0 835 1761
Size: 835 926
HaeII r_GCGC'y
Cuts at: 0 1547 1761
Size: 1547 214
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 480 512 935 967 1419 1440 1451 1472
Size: 480 32 423 32 452 21 11 21
Cuts at: 1472 1761
Size: 289
Fragments arranged by size:
480 452 423 289 32 32 21 21
11
KasI G'GCGC_C
Cuts at: 0 1543 1761
Size: 1543 218
MfeI C'AATT_G
Cuts at: 0 962 1723 1761
Size: 962 761 38
Fragments arranged by size:
962 761 38
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 219 1293 1761
Size: 219 1074 468
Fragments arranged by size:
1074 468 219
MslI CAynn'nnrTG
Cuts at: 0 227 1761
Size: 227 1534
MspA1I CmG'CkG
Cuts at: 0 408 1761
Size: 408 1353
NarI GG'CG_CC
Cuts at: 0 1544 1761
Size: 1544 217
NdeI CA'TA_TG
Cuts at: 0 1743 1761
Size: 1743 18
NsiI A_TGCA'T
Cuts at: 0 1587 1761
Size: 1587 174
NspI r_CATG'y
Cuts at: 0 1374 1761
Size: 1374 387
PflMI CCAn_nnn'nTGG
Cuts at: 0 312 946 1761
Size: 312 634 815
Fragments arranged by size:
815 634 312
PspOMI G'GGCC_C
Cuts at: 0 1078 1761
Size: 1078 683
PstI C_TGCA'G
Cuts at: 0 253 552 1761
Size: 253 299 1209
Fragments arranged by size:
1209 299 253
PvuII CAG'CTG
Cuts at: 0 408 1761
Size: 408 1353
SapI GCTCTTCn'nnn_
Cuts at: 0 503 1761
Size: 503 1258
ScaI AGT'ACT
Cuts at: 0 1653 1761
Size: 1653 108
SexAI A'CCwGG_T
Cuts at: 0 945 1761
Size: 945 816
SfcI C'TryA_G
Cuts at: 0 249 548 1034 1761
Size: 249 299 486 727
Fragments arranged by size:
727 486 299 249
SfoI GGC'GCC
Cuts at: 0 1545 1761
Size: 1545 216
SmlI C'TyrA_G
Cuts at: 0 1086 1500 1761
Size: 1086 414 261
Fragments arranged by size:
1086 414 261
SspI AAT'ATT
Cuts at: 0 611 1761
Size: 611 1150
SwaI ATTT'AAAT
Cuts at: 0 1268 1761
Size: 1268 493
TatI w'GTAC_w
Cuts at: 0 705 1651 1761
Size: 705 946 110
Fragments arranged by size:
946 705 110
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 552 584 1761
Size: 552 32 1177
Fragments arranged by size:
1177 552 32
Tth111I GACn'n_nGTC
Cuts at: 0 947 1309 1761
Size: 947 362 452
Fragments arranged by size:
947 452 362
XmnI GAAnn'nnTTC
Cuts at: 0 611 1751 1761
Size: 611 1140 10
Fragments arranged by size:
1140 611 10
Enzymes that do cut:
AcuI AlwNI ApaI ApoI BaeI BanI BanII BbeI
BciVI BfrBI BglI Bme1580I Bpu10I BpuEI BsaI BsaHI
BsaWI BsaXI BseRI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BsrGI BstYI BtsI CspCI DraI EarI Eco57MI
EcoRV HaeII Hin4I KasI MfeI MmeI MslI MspA1I
NarI NdeI NsiI NspI PflMI PspOMI PstI PvuII
SapI ScaI SexAI SfcI SfoI SmlI SspI SwaI
TatI TstI Tth111I XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI AscI AseI
AsiSI AvaI AvrII BamHI BbsI BbvCI BcgI BclI
BglII BlpI BmgBI BmrI BmtI BplI BpmI BsaAI
BsaBI BseYI BsiEI BsiWI BspEI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtgI BtgZI ClaI DraIII DrdI EaeI EagI
EciI EcoICRI EcoNI EcoO109I EcoRI FalI FseI FspI
FspAI HincII HindIII HpaI KpnI MluI MscI NaeI
NcoI NgoMIV NheI NotI NruI PacI PasI PciI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SbfI
SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
StuI StyI TaqII XbaI XcmI XhoI XmaI ZraI