Restriction map for pF1KB6845
 (Circular) (Six-base) MAPSORT of: pF1KB6845.seq  Check: 785  from: 1  to: 3638



pF1KB6845  3638 bp

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

AccI GT'mk_AC

Cuts at:    676     676 
   Size:       3638
 
Acc65I G'GTAC_C

Cuts at:    654     654 
   Size:       3638
 
AclI AA'CG_TT

Cuts at:   3478    3478 
   Size:       3638
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1463    1895    2642    2910    1463 
   Size:        432     747     268    2191

  Fragments arranged by size: 

                2191     747     432     268
 
AflIII A'CryG_T

Cuts at:    990    2094    3071     990 
   Size:       1104     977    1557

  Fragments arranged by size: 

                1557    1104     977
 
AgeI A'CCGG_T

Cuts at:   2026    2026 
   Size:       3638
 
AlwNI CAG_nnn'CTG

Cuts at:    943    2510     943 
   Size:       1567    2071
 
ApaLI G'TGCA_C

Cuts at:   2408    2408 
   Size:       3638
 
ApoI r'AATT_y

Cuts at:    642    2991    3247     642 
   Size:       2349     256    1033

  Fragments arranged by size: 

                2349    1033     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3638
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3638
 
AvaI C'yCGr_G

Cuts at:    658     658 
   Size:       3638
 
BamHI G'GATC_C

Cuts at:    218     361     663     218 
   Size:        143     302    3193

  Fragments arranged by size: 

                3193     302     143
 
BanI G'GyrC_C

Cuts at:    442     654    1068    1316    1351     442 
   Size:        212     414     248      35    2729

  Fragments arranged by size: 

                2729     414     248     212      35
 
BanII G_rGCy'C

Cuts at:    425     452     652    1682     425 
   Size:         27     200    1030    2381

  Fragments arranged by size: 

                2381    1030     200      27
 
BbeI G_GCGC'C

Cuts at:   1072    1320    1072 
   Size:        248    3390
 
BbvCI CC'TCA_GC

Cuts at:    356     356 
   Size:       3638
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:     90     124      90 
   Size:         34    3604
 
BciVI GTATCCnnnnn_n'

Cuts at:   1536    2297    3638    1536 
   Size:        761    1341    1536

  Fragments arranged by size: 

                1536    1341     761
 
BclI T'GATC_A

Cuts at:   1158    1158 
   Size:       3638
 
BglII A'GATC_T

Cuts at:    300    1153    3190     300 
   Size:        853    2037     748

  Fragments arranged by size: 

                2037     853     748
 
BlpI GC'TnA_GC

Cuts at:    750     750 
   Size:       3638
 
Bme1580I G_kGCm'C

Cuts at:    445    1263    1356    2412     445 
   Size:        818      93    1056    1671

  Fragments arranged by size: 

                1671    1056     818      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    125     850     931    1266    3410     125 
   Size:        725      81     335    2144     353

  Fragments arranged by size: 

                2144     725     353     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2005    2005 
   Size:       3638
 
Bpu10I CC'TnA_GC

Cuts at:    356     356 
   Size:       3638
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    513     814    1693    2185    2483     513 
   Size:        301     879     492     298    1668

  Fragments arranged by size: 

                1668     879     492     301     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3342    3342 
   Size:       3638
 
BsaAI yAC'GTr

Cuts at:    991     991 
   Size:       3638
 
BsaBI GATnn'nnATC

Cuts at:      6     299    1152    3189       6 
   Size:        293     853    2037     455

  Fragments arranged by size: 

                2037     853     455     293
 
BsaHI Gr'CG_yC

Cuts at:   1069    1317    2019    3515    1069 
   Size:        248     702    1496    1192

  Fragments arranged by size: 

                1496    1192     702     248
 
BsaWI w'CCGG_w

Cuts at:    827    1348    2026    2300    2447    2858     827 
   Size:        521     678     274     147     411    1607

  Fragments arranged by size: 

                1607     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    465     495     465 
   Size:         30    3608
 
BseYI C'CCAG_C

Cuts at:   1063    1821    2398    2894    1063 
   Size:        758     577     496    1807

  Fragments arranged by size: 

                1807     758     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    197     197 
   Size:       3638
 
BsiEI CG_ry'CG

Cuts at:    104    1180    1226    2434     104 
   Size:       1076      46    1208    1308

  Fragments arranged by size: 

                1308    1208    1076      46
 
BsiHKAI G_wGCw'C

Cuts at:    347     652    1430    1620    2412     347 
   Size:        305     778     190     792    1573

  Fragments arranged by size: 

                1573     792     778     305     190
 
Bsp1286I G_dGCh'C

Cuts at:    347     425     445     452     652    1263    1356    1430    1620 
   Size:         78      20       7     200     611      93      74     190

Cuts at:   1620    1682    2412     347 
   Size:         62     730    1573

  Fragments arranged by size: 

                1573     730     611     200     190      93      78      74
                  62      20       7
 
BspEI T'CCGG_A

Cuts at:   2858    2858 
   Size:       3638
 
BspHI T'CATG_A

Cuts at:   2814    2814 
   Size:       3638
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    688     902    1204    1585     688 
   Size:        214     302     381    2741

  Fragments arranged by size: 

                2741     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1930    1984    3468    3633      50 
   Size:       1880      54    1484     165      55

  Fragments arranged by size: 

                1880    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1550    1550 
   Size:       3638
 
BsrFI r'CCGG_y

Cuts at:   1636    1817    2026    1636 
   Size:        181     209    3248

  Fragments arranged by size: 

                3248     209     181
 
BssSI C'ACGA_G

Cuts at:   1909    2267    1909 
   Size:        358    3280
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1015    1015 
   Size:       3638
 
BstBI TT'CG_AA

Cuts at:   1999    1999 
   Size:       3638
 
BstEII G'GTnAC_C

Cuts at:    605     605 
   Size:       3638
 
BstXI CCAn_nnnn'nTGG

Cuts at:    287     287 
   Size:       3638
 
BstYI r'GATC_y

Cuts at:      1     218     300     361     663    1130    1153    1488    1734 
   Size:        217      82      61     302     467      23     335     246

Cuts at:   1734    2746    2832    2844    3190       1 
   Size:       1012      86      12     346     449

  Fragments arranged by size: 

                1012     467     449     346     335     302     246     217
                  86      82      61      23      12
 
BtgI C'CryG_G

Cuts at:    119     119 
   Size:       3638
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1588    1769    1932    1588 
   Size:        181     163    3294

  Fragments arranged by size: 

                3294     181     163
 
BtsI GCAGTG_nn'

Cuts at:    255     486     881     255 
   Size:        231     395    3012

  Fragments arranged by size: 

                3012     395     231
 
DraI TTT'AAA

Cuts at:    637     637 
   Size:       3638
 
DrdI GACnn_nn'nnGTC

Cuts at:   1344    2202    1344 
   Size:        858    2780
 
EaeI y'GGCC_r

Cuts at:    537    1223    1397    1788    1815    3160     537 
   Size:        686     174     391      27    1345    1015

  Fragments arranged by size: 

                1345    1015     686     391     174      27
 
EagI C'GGCC_G

Cuts at:   1223    1223 
   Size:       3638
 
EarI CTCTTCn'nnn_

Cuts at:    379    1661    1871     379 
   Size:       1282     210    2146

  Fragments arranged by size: 

                2146    1282     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1846    2156    2302    3448    1846 
   Size:        310     146    1146    2036

  Fragments arranged by size: 

                2036    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    650     650 
   Size:       3638
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1463    1895    2642    2910    1463 
   Size:        432     747     268    2191

  Fragments arranged by size: 

                2191     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    777     777 
   Size:       3638
 
EcoRI G'AATT_C

Cuts at:    642     642 
   Size:       3638
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1657    1657 
   Size:       3638
 
FspI TGC'GCA

Cuts at:   1419    1419 
   Size:       3638
 
HaeII r_GCGC'y

Cuts at:   1072    1320    2342    3321    1072 
   Size:        248    1022     979    1389

  Fragments arranged by size: 

                1389    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    210     242    1480    1510    1512    1542     210 
   Size:         32    1238      30       2      30    2306

  Fragments arranged by size: 

                2306    1238      32      30      30       2
 
HincII GTy'rAC

Cuts at:    677     677 
   Size:       3638
 
KasI G'GCGC_C

Cuts at:   1068    1316    1068 
   Size:        248    3390
 
KpnI G_GTAC'C

Cuts at:    658     658 
   Size:       3638
 
MluI A'CGCG_T

Cuts at:   3071    3071 
   Size:       3638
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2309    2493    2309 
   Size:        184    3454
 
MscI TGG'CCA

Cuts at:   1399    1399 
   Size:       3638
 
MslI CAynn'nnrTG

Cuts at:    260    1627    1754     260 
   Size:       1367     127    2144

  Fragments arranged by size: 

                2144    1367     127
 
MspA1I CmG'CkG

Cuts at:    224     749    1063    1423    2436    2681     224 
   Size:        525     314     360    1013     245    1181

  Fragments arranged by size: 

                1181    1013     525     360     314     245
 
NaeI GCC'GGC

Cuts at:   1819    1819 
   Size:       3638
 
NarI GG'CG_CC

Cuts at:   1069    1317    1069 
   Size:        248    3390
 
NcoI C'CATG_G

Cuts at:    119     119 
   Size:       3638
 
NgoMIV G'CCGG_C

Cuts at:   1817    1817 
   Size:       3638
 
NspI r_CATG'y

Cuts at:    691    1191    2098     691 
   Size:        500     907    2231

  Fragments arranged by size: 

                2231     907     500
 
PciI A'CATG_T

Cuts at:   2094    2094 
   Size:       3638
 
PfoI T'CCnGG_A

Cuts at:    286     286 
   Size:       3638
 
PmeI GTTT'AAAC

Cuts at:    637     637 
   Size:       3638
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2777    2809    2777 
   Size:         32    3606
 
PsiI TTA'TAA

Cuts at:    590     590 
   Size:       3638
 
PstI C_TGCA'G

Cuts at:    685    1370     685 
   Size:        685    2953
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3638
 
PvuII CAG'CTG

Cuts at:   1063    1423    1063 
   Size:        360    3278
 
RsrII CG'GwC_CG

Cuts at:   1833    1833 
   Size:       3638
 
SacI G_AGCT'C

Cuts at:    652     652 
   Size:       3638
 
SalI G'TCGA_C

Cuts at:    675     675 
   Size:       3638
 
SapI GCTCTTCn'nnn_

Cuts at:    379    1661    1871     379 
   Size:       1282     210    2146

  Fragments arranged by size: 

                2146    1282     210
 
SbfI CC_TGCA'GG

Cuts at:    685     685 
   Size:       3638
 
ScaI AGT'ACT

Cuts at:   3155    3155 
   Size:       3638
 
SexAI A'CCwGG_T

Cuts at:    332     332 
   Size:       3638
 
SfcI C'TryA_G

Cuts at:     33     681    1366    2359    2550      33 
   Size:        648     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     648     191
 
SfoI GGC'GCC

Cuts at:   1070    1318    1070 
   Size:        248    3390
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3638
 
SmaI CCC'GGG

Cuts at:    660     660 
   Size:       3638
 
SmlI C'TyrA_G

Cuts at:    528     793    1708    2200    2462     528 
   Size:        265     915     492     262    1704

  Fragments arranged by size: 

                1704     915     492     265     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3638
 
SphI G_CATG'C

Cuts at:    691    1191     691 
   Size:        500    3138
 
StuI AGG'CCT

Cuts at:    244     244 
   Size:       3638
 
StyI C'CwwG_G

Cuts at:    119     239     772    1126     119 
   Size:        120     533     354    2631

  Fragments arranged by size: 

                2631     533     354     120
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1221    1354    1813    2022    1221 
   Size:        133     459     209    2837

  Fragments arranged by size: 

                2837     459     209     133
 
TatI w'GTAC_w

Cuts at:   3153    3153 
   Size:       3638
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    320     352     353     385     465     497    3155    3187     320 
   Size:         32       1      32      80      32    2658      32     771

  Fragments arranged by size: 

                2658     771      80      32      32      32      32       1
            
 
Tth111I GACn'n_nGTC

Cuts at:   1435    1435 
   Size:       3638
 
XbaI T'CTAG_A

Cuts at:    669     669 
   Size:       3638
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1133    1133 
   Size:       3638
 
XmaI C'CCGG_G

Cuts at:    658     658 
   Size:       3638
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
    BbvCI     BcgI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI
     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI
     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI
     PciI     PfoI     PmeI     PpiI     PsiI     PstI     PvuI    PvuII
    RsrII     SacI     SalI     SapI     SbfI     ScaI    SexAI     SfcI
     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI     BbsI    BfrBI     BglI    BmgBI
     BmtI     BpmI    BseRI    BsiWI     BsmI    BsmBI    BsrGI   BssHII
  BstZ17I   Bsu36I     ClaI    CspCI   DraIII    EcoNI    EcoRV     FseI
    FspAI  HindIII     HpaI     MfeI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PasI    PflMI     PmlI    PpuMI    PshAI   PspOMI
    PspXI     PsrI    SacII    SanDI     SfiI    SgrAI    SnaBI     SrfI
     SspI     SwaI     XhoI     XmnI     ZraI
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