Restriction map for pF1KB6395
 (Linear) (Six-base) MAPSORT of: KIBB6395.seq  Check: 8850  from: 1  to: 828



KIBB6395.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:46  ..

AclI AA'CG_TT

Cuts at:      0     191     828 
   Size:        191     637
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     683     828 
   Size:        683     145
 
AlwNI CAG_nnn'CTG

Cuts at:      0     168     828 
   Size:        168     660
 
ApoI r'AATT_y

Cuts at:      0     154     828 
   Size:        154     674
 
AvaI C'yCGr_G

Cuts at:      0      29     134     269     828 
   Size:         29     105     135     559

  Fragments arranged by size: 

                 559     135     105      29
 
BanI G'GyrC_C

Cuts at:      0     214     343     828 
   Size:        214     129     485

  Fragments arranged by size: 

                 485     214     129
 
BanII G_rGCy'C

Cuts at:      0      27     125     828 
   Size:         27      98     703

  Fragments arranged by size: 

                 703      98      27
 
BbvCI CC'TCA_GC

Cuts at:      0     276     828 
   Size:        276     552
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     387     828 
   Size:        387     441
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     101     746     828 
   Size:        101     645      82

  Fragments arranged by size: 

                 645     101      82
 
Bpu10I CC'TnA_GC

Cuts at:      0     276     828 
   Size:        276     552
 
BsaAI yAC'GTr

Cuts at:      0     555     828 
   Size:        555     273
 
BsaHI Gr'CG_yC

Cuts at:      0      75     828 
   Size:         75     753
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     719     749     828 
   Size:        719      30      79

  Fragments arranged by size: 

                 719      79      30
 
BseYI C'CCAG_C

Cuts at:      0     280     524     828 
   Size:        280     244     304

  Fragments arranged by size: 

                 304     280     244
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     380     787     828 
   Size:        380     407      41

  Fragments arranged by size: 

                 407     380      41
 
BsiEI CG_ry'CG

Cuts at:      0      43     828 
   Size:         43     785
 
BsiHKAI G_wGCw'C

Cuts at:      0     125     828 
   Size:        125     703
 
BsmI GAATG_Cn'

Cuts at:      0     146     406     828 
   Size:        146     260     422

  Fragments arranged by size: 

                 422     260     146
 
Bsp1286I G_dGCh'C

Cuts at:      0      27     125     828 
   Size:         27      98     703

  Fragments arranged by size: 

                 703      98      27
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     344     806     828 
   Size:        344     462      22

  Fragments arranged by size: 

                 462     344      22
 
BtgI C'CryG_G

Cuts at:      0     210     596     828 
   Size:        210     386     232

  Fragments arranged by size: 

                 386     232     210
 
BtsI GCAGTG_nn'

Cuts at:      0     266     828 
   Size:        266     562
 
EaeI y'GGCC_r

Cuts at:      0      40     392     681     828 
   Size:         40     352     289     147

  Fragments arranged by size: 

                 352     289     147      40
 
EagI C'GGCC_G

Cuts at:      0      40     828 
   Size:         40     788
 
EarI CTCTTCn'nnn_

Cuts at:      0     659     828 
   Size:        659     169
 
EcoICRI GAG'CTC

Cuts at:      0     123     828 
   Size:        123     705
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     101     683     746     828 
   Size:        101     582      63      82

  Fragments arranged by size: 

                 582     101      82      63
 
EcoO109I rG'GnC_Cy

Cuts at:      0     284     538     828 
   Size:        284     254     290

  Fragments arranged by size: 

                 290     284     254
 
EcoRI G'AATT_C

Cuts at:      0     154     828 
   Size:        154     674
 
HaeII r_GCGC'y

Cuts at:      0       7     387     828 
   Size:          7     380     441

  Fragments arranged by size: 

                 441     380       7
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     719     751     828 
   Size:        719      32      77

  Fragments arranged by size: 

                 719      77      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     491     828 
   Size:        491     337
 
MscI TGG'CCA

Cuts at:      0     394     828 
   Size:        394     434
 
MspA1I CmG'CkG

Cuts at:      0      39     280     828 
   Size:         39     241     548

  Fragments arranged by size: 

                 548     241      39
 
NcoI C'CATG_G

Cuts at:      0     210     828 
   Size:        210     618
 
PasI CC'CwG_GG

Cuts at:      0     168     828 
   Size:        168     660
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     168     828 
   Size:        168     660
 
PfoI T'CCnGG_A

Cuts at:      0     540     828 
   Size:        540     288
 
PpuMI rG'GwC_Cy

Cuts at:      0     284     538     828 
   Size:        284     254     290

  Fragments arranged by size: 

                 290     284     254
 
PvuII CAG'CTG

Cuts at:      0     280     828 
   Size:        280     548
 
SacI G_AGCT'C

Cuts at:      0     125     828 
   Size:        125     703
 
ScaI AGT'ACT

Cuts at:      0     376     828 
   Size:        376     452
 
SfcI C'TryA_G

Cuts at:      0      96     828 
   Size:         96     732
 
SmaI CCC'GGG

Cuts at:      0     136     828 
   Size:        136     692
 
StuI AGG'CCT

Cuts at:      0     670     828 
   Size:        670     158
 
StyI C'CwwG_G

Cuts at:      0     210     395     678     828 
   Size:        210     185     283     150

  Fragments arranged by size: 

                 283     210     185     150
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     160     756     828 
   Size:        160     596      72

  Fragments arranged by size: 

                 596     160      72
 
TatI w'GTAC_w

Cuts at:      0     374     410     781     828 
   Size:        374      36     371      47

  Fragments arranged by size: 

                 374     371      47      36
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     169     828 
   Size:        169     659
 
XmaI C'CCGG_G

Cuts at:      0     134     828 
   Size:        134     694
 

 Enzymes that do cut:

     AclI     AcuI    AlwNI     ApoI     AvaI     BanI    BanII    BbvCI
     BmrI     BpmI   Bpu10I    BsaAI    BsaHI    BsaXI    BseYI     BsgI
    BsiEI  BsiHKAI     BsmI Bsp1286I    BspMI     BtgI     BtsI     EaeI
     EagI     EarI  EcoICRI  Eco57MI EcoO109I    EcoRI    HaeII    Hin4I
     MmeI     MscI   MspA1I     NcoI     PasI    PflMI     PfoI    PpuMI
    PvuII     SacI     ScaI     SfcI     SmaI     StuI     StyI    TaqII
     TatI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BbeI     BbsI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmtI
     BplI    BpuEI     BsaI    BsaBI    BsaWI    BseRI    BsiWI    BsmBI
    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EciI    EcoNI    EcoRV
     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MslI     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PciI     PmeI
     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    RsrII    SacII     SalI    SanDI     SapI     SbfI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI     TstI  Tth111I     XbaI     XhoI     XmnI
     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com