Restriction map for pF1KB6385
##################################
# NO CUT ENZYMES (  117 enzymes) #
##################################

AatII       AccIII      AclI        AcsI        AflII       
AhdI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     AspEI       AviII       AvrII       BamHI       
BbvCI       BcgI        BciVI       BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BsaBI       
BsaI        BseAI       BseRI       BsiWI       BsmAI       
BsmBI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
DraI        DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoRI       
EcoRV       FseI        FspI        HincII      HindII      
HindIII     HpaI        MamI        MfeI        MroI        
MseI        MunI        NaeI        NdeI        NgoMIV      
NheI        NotI        NruI        NspV        PacI        
PaeR7I      PflMI       PleI        PmeI        PshAI       
PshBI       Psp1406I    PstI        PvuI        RcaI        
RsrII       SalI        SbfI        ScaI        SexAI       
SfiI        SfuI        SgrAI       SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        Tru9I       Tsp509I     Van91I      XbaI        
XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   60 enzymes) #
###################################

Acc65I        383  GGTACC               G^GTACC
AccI          424  GTAGAC               GT^[AC][GT]AC
AfaI          384  GTAC                 GT^AC
AgeI          536  ACCGGT               A^CCGGT
AlwNI         571  CAGGGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp718I       383  GGTACC               G^GTACC
AspHI         684  GAGCTC               G[AT]GC[AT]^C
AspI          145  GACGGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI          344  TGGCCA               TGG^CCA
BbrPI         394  CACGTG               CAC^GTG
BbsI          532  GAAGAC               GAAGAC(2/6)
BclI          647  TGATCA               T^GATCA
BpuAI         532  GAAGAC               GAAGAC(2/6)
BsaAI         394  CACGTG               [CT]AC^GT[GA]
BsaWI         536  ACCGGT               [AT]^CCGG[AT]
BsgI          799  GTGCAG               GTGCAG(16/14)
BsiEI         188  CGGTCG               CG[GA][CT]^CG
BsiHKAI       684  GAGCTC               G[AT]GC[AT]^C
BsrDI         629  GCAATG               GCAATG(2/0)
BsrFI         536  ACCGGT               [GA]^CCGG[CT]
BstEII        610  GGTGACC              G^GT[ACGT]ACC
BstPI         610  GGTGACC              G^GT[ACGT]ACC
BstXI         281  CCACACGGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I        536  ACCGGT               [GA]^CCGG[CT]
DraII          92  AGGTCCC              [GA]G^G[ACGT]CC[CT]
DraIII        724  CACTTTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I       92  AGGTCCC              [GA]G^G[ACGT]CC[CT]
EcoO65I       610  GGTGACC              G^GT[ACGT]ACC
EcoT14I       462  CCATGG               C^C[AT][AT]GG
EcoT22I        59  ATGCAT               ATGCA^T
FbaI          647  TGATCA               T^GATCA
HinfI         184  GATTC                G^A[ACGT]TC
KpnI          383  GGTACC               GGTAC^C
KspI          263  CCGCGG               CCGC^GG
MaeII         395  ACGT                 A^CGT
MluNI         344  TGGCCA               TGG^CCA
MscI          344  TGGCCA               TGG^CCA
NcoI          462  CCATGG               C^CATGG
NsiI           59  ATGCAT               ATGCA^T
NspI          640  GCATGT               [GA]CATG^[CT]
PflFI         145  GACGGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI         536  ACCGGT               A^CCGGT
PmaCI         394  CACGTG               CAC^GTG
PmlI          394  CACGTG               CAC^GTG
PpuMI          92  AGGTCCC              [GA]G^G[AT]CC[CT]
PvuII         743  CAGCTG               CAG^CTG
RsaI          384  GTAC                 GT^AC
SacI          684  GAGCTC               GAGCT^C
SacII         263  CCGCGG               CCGC^GG
SapI          375  GCTCTTC              GCTCTTC(1/4)
SfcI          471  CTACAG               C^T[GA][CT]AG
SmaI           81  CCCGGG               CCC^GGG
SmlI           88  CTCAAG               C^T[CT][GA]AG
StyI          462  CCATGG               C^C[AT][AT]GG
TaqI           41  TCGA                 T^CGA
TfiI          184  GATTC                G^A[AT]TC
Tth111I       145  GACGGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I        41  TCGA                 T^CGA
XmaCI          81  CCCGGG               C^CCGGG
XmaI           81  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AflIII         72  ACGCGT               A^C[GA][CT]GT
              314  ACGCGT              
AlwI          216  GATCC                GGATC(4/5)
              602  GGATC               
AvaI           23  CCCGAG               C^[CT]CG[GA]G
               81  CCCGGG              
BbeI          160  GGCGCC               GGCGC^C
              661  GGCGCC              
BfaI          529  CTAG                 C^TAG
              784  CTAG                
BsmFI         115  GGGAC                GGGAC(10/14)
               94  GTCCC               
BsoBI          23  CCCGAG               C^[CT]CG[GA]G
               81  CCCGGG              
BsrI          433  ACTGG                ACTGG(1/-1)
              658  ACTGG               
BstYI         215  AGATCC               [GA]^GATC[CT]
              602  GGATCT              
DdeI          569  CTCAG                C^T[ACGT]AG
              682  CTGAG               
EaeI          344  TGGCCA               [CT]^GGCC[GA]
              714  TGGCCG              
EarI          376  CTCTTC               CTCTTC(1/4)
              705  GAAGAG              
FauI          338  CCCGC                CCCGC(4/6)
              127  GCGGG               
HaeII         160  GGCGCC               [GA]GCGC^[CT]
              661  GGCGCC              
KasI          160  GGCGCC               G^GCGCC
              661  GGCGCC              
Ksp632I       376  CTCTTC               CTCTTC(1/4)
              705  GAAGAG              
MaeI          529  CTAG                 C^TAG
              784  CTAG                
MflI          215  AGATCC               [GA]^GATC[CT]
              602  GGATCT              
MluI           72  ACGCGT               A^CGCGT
              314  ACGCGT              
MspA1I        263  CCGCGG               C[AC]G^C[GT]G
              743  CAGCTG              
NarI          160  GGCGCC               GG^CGCC
              661  GGCGCC              
SfoI          160  GGCGCC               GGC^GCC
              661  GGCGCC              
TspRI         680  CACTG                CA[GC]TG[ACGT][ACGT]^
              761  CAGTG               
XhoII         215  AGATCC               [GA]^GATC[CT]
              602  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   57 enzymes) #
#########################################

AccII       AciI        AcyI        AluI        AvaII       
BanI        BanII       BbvI        BcnI        BmyI        
BpmI        BsaHI       BsaJI       BsiYI       BslI        
Bsp1286I    BssKI       BstF5I      BstNI       BstUI       
Cac8I       CfoI        Cfr13I      DpnII       DsaI        
EcoRII      Fnu4HI      FokI        HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HpaII       
HphI        ItaI        MaeIII      MboI        MboII       
MnlI        MslI        MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
Sau3AI      Sau96I      ScrFI       SfaNI       TseI        
Tsp45I      XcmI        
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