Restriction map for pF1KE0481
##################################
# NO CUT ENZYMES (  147 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
AscI        Asp718I     AspEI       AspHI       AviII       
AvrII       BalI        BanI        BbeI        BbrPI       
BbsI        BbvCI       BcgI        BciVI       BclI        
BfrI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaWI       BseAI       BsiEI       BsiHKAI     BsiWI       
BsmBI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrDI       BsrGI       
BssHII      BstAPI      BstBI       BstEII      BstPI       
BstUI       BstXI       Bsu36I      CelII       ClaI        
CpoI        DraIII      DrdI        EaeI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRV       
FbaI        FseI        FspI        HaeII       Hin1I       
HincII      HindII      HindIII     HpaI        KasI        
KpnI        Ksp632I     KspI        MamI        MfeI        
MluI        MluNI       MroI        MscI        MslI        
MspA1I      MunI        MvnI        NaeI        NarI        
NgoMIV      NheI        NotI        NruI        NspI        
NspV        PacI        PaeR7I      PflMI       PinAI       
PmaCI       PmeI        PmlI        PshAI       Psp1406I    
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        Van91I      
XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AcsI         1007  GAATTT               [GA]^AATT[CT]
AfaI          201  GTAC                 GT^AC
AlwNI        1196  CAGCCACTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI         1007  GAATTT               [GA]^AATT[CT]
AseI         1102  ATTAAT               AT^TAAT
AsnI         1102  ATTAAT               AT^TAAT
Asp700I      1108  GAAAAGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI          338  CCCGGG               C^[CT]CG[GA]G
BfaI          392  CTAG                 C^TAG
BsaI          478  GAGACC               GGTCTC(1/5)
BsgI           24  CTGCAC               GTGCAG(16/14)
BsiYI         809  CCTACATTTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          809  CCTACATTTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsoBI         338  CCCGGG               C^[CT]CG[GA]G
BspMI          86  ACCTGC               ACCTGC(4/8)
BssSI         900  CACGAG               CACGAG(-5/-1)
Bst1107I      627  GTATAC               GTA^TAC
BstZ17I       627  GTATAC               GTA^TAC
CfoI           33  GCGC                 GCG^C
DraI           72  TTTAAA               TTT^AAA
DsaI          482  CCATGG               C^C[GA][CT]GG
EcoT14I       482  CCATGG               C^C[AT][AT]GG
EcoT22I       311  ATGCAT               ATGCA^T
FauI         1166  GCGGG                CCCGC(4/6)
HaeIII        275  GGCC                 GG^CC
HhaI           33  GCGC                 GCG^C
HinP1I         33  GCGC                 G^CGC
MaeI          392  CTAG                 C^TAG
MaeII         408  ACGT                 A^CGT
MboII         153  TCTTC                GAAGA(8/7)
NcoI          482  CCATGG               C^CATGG
NdeI          242  CATATG               CA^TATG
NsiI          311  ATGCAT               ATGCA^T
PshBI        1102  ATTAAT               AT^TAAT
PstI          632  CTGCAG               CTGCA^G
RsaI          201  GTAC                 GT^AC
SfcI          632  CTGCAG               C^T[GA][CT]AG
SmaI          338  CCCGGG               CCC^GGG
StyI          482  CCATGG               C^C[AT][AT]GG
TaqI         1232  TCGA                 T^CGA
TthHB8I      1232  TCGA                 T^CGA
XcmI          473  CCAGGGAGACCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI         338  CCCGGG               C^CCGGG
XmaI          338  CCCGGG               C^CCGGG
XmnI         1108  GAAAAGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AccI          250  GTAGAC               GT^[AC][GT]AC
              627  GTATAC              
AspI          685  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              964  GACCCAGTC           
BamHI         352  GGATCC               G^GATCC
              878  GGATCC              
BanII          49  GGGCTC               G[GA]GC[CT]^C
             1168  GGGCTC              
BbvI          458  GCAGC                GCAGC(8/12)
              714  GCTGC               
BmyI           49  GGGCTC               G[AGT]GC[ACT]^C
             1168  GGGCTC              
BseRI         703  CTCCTC               GAGGAG(10/8)
              395  GAGGAG              
Bsp1286I       49  GGGCTC               G[AGT]GC[ACT]^C
             1168  GGGCTC              
BsrFI         187  ACCGGC               [GA]^CCGG[CT]
             1080  ACCGGC              
BstYI         352  GGATCC               [GA]^GATC[CT]
              878  GGATCC              
Cfr10I        187  ACCGGC               [GA]^CCGG[CT]
             1080  ACCGGC              
HgaI          346  GACGC                GACGC(5/10)
              523  GACGC               
MflI          352  GGATCC               [GA]^GATC[CT]
              878  GGATCC              
NlaIII        483  CATG                 CATG^
             1224  CATG                
PflFI         685  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              964  GACCCAGTC           
TfiI          180  GAATC                G^A[AT]TC
              699  GAATC               
TseI          458  GCAGC                G^C[AT]GC
              714  GCTGC               
Tth111I       685  GACCCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              964  GACCCAGTC           
XhoII         352  GGATCC               [GA]^GATC[CT]
              878  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   47 enzymes) #
#########################################

AciI        AluI        AlwI        AvaII       BcnI        
BpmI        BsaJI       BsmAI       BsmFI       BsrI        
BssKI       BstF5I      BstNI       Cac8I       Cfr13I      
DdeI        DpnII       DraII       EcoO109I    EcoRII      
Fnu4HI      FokI        HapII       HinfI       HpaII       
HphI        ItaI        MaeIII      MboI        MnlI        
MseI        MspI        MvaI        MwoI        NciI        
NdeII       NlaIV       PleI        PpuMI       Sau3AI      
Sau96I      ScrFI       SfaNI       Tru9I       Tsp45I      
Tsp509I     TspRI       
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