Restriction map for pF1KE0481
 (Circular) (Six-base) MAPSORT of: pF1KE0481.seq  Check: 5936  from: 1  to: 4370



pF1KE0481  4370 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:15  ..

AccI GT'mk_AC

Cuts at:    359     736    1408     359 
   Size:        377     672    3321

  Fragments arranged by size: 

                3321     672     377
 
Acc65I G'GTAC_C

Cuts at:   1386    1386 
   Size:       4370
 
AclI AA'CG_TT

Cuts at:   4210    4210 
   Size:       4370
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   2195    2627    3374    3642    2195 
   Size:        432     747     268    2923

  Fragments arranged by size: 

                2923     747     432     268
 
AflIII A'CryG_T

Cuts at:   1722    2826    3803    1722 
   Size:       1104     977    2289

  Fragments arranged by size: 

                2289    1104     977
 
AgeI A'CCGG_T

Cuts at:   2758    2758 
   Size:       4370
 
AlwNI CAG_nnn'CTG

Cuts at:   1309    1675    3242    1309 
   Size:        366    1567    2437

  Fragments arranged by size: 

                2437    1567     366
 
ApaLI G'TGCA_C

Cuts at:   3140    3140 
   Size:       4370
 
ApoI r'AATT_y

Cuts at:   1115    1374    3723    3979    1115 
   Size:        259    2349     256    1506

  Fragments arranged by size: 

                2349    1506     259     256
 
AseI AT'TA_AT

Cuts at:     20    1211      20 
   Size:       1191    3179
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4370
 
AvaI C'yCGr_G

Cuts at:    446    1390     446 
   Size:        944    3426
 
BamHI G'GATC_C

Cuts at:    460     986    1395     460 
   Size:        526     409    3435

  Fragments arranged by size: 

                3435     526     409
 
BanI G'GyrC_C

Cuts at:   1386    1800    2048    2083    1386 
   Size:        414     248      35    3673

  Fragments arranged by size: 

                3673     414     248      35
 
BanII G_rGCy'C

Cuts at:    161    1280    1384    2414     161 
   Size:       1119     104    1030    2117

  Fragments arranged by size: 

                2117    1119    1030     104
 
BbeI G_GCGC'C

Cuts at:   1804    2052    1804 
   Size:        248    4122
 
BciVI GTATCCnnnnn_n'

Cuts at:   2268    3029    4370    2268 
   Size:        761    1341    2268

  Fragments arranged by size: 

                2268    1341     761
 
BclI T'GATC_A

Cuts at:   1890    1890 
   Size:       4370
 
BfrBI ATG'CAT

Cuts at:    421     421 
   Size:       4370
 
BglII A'GATC_T

Cuts at:   1885    3922    1885 
   Size:       2037    2333
 
BlpI GC'TnA_GC

Cuts at:   1482    1482 
   Size:       4370
 
Bme1580I G_kGCm'C

Cuts at:   1995    2088    3144    1995 
   Size:         93    1056    3221

  Fragments arranged by size: 

                3221    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    766     790     995    1069    1582    1663    1998    4142     766 
   Size:         24     205      74     513      81     335    2144     994

  Fragments arranged by size: 

                2144     994     513     335     205      81      74      24
            
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1171    2737    1171 
   Size:       1566    2804
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    546     559     611     546 
   Size:         13      52    4305

  Fragments arranged by size: 

                4305      52      13
 
Bpu10I CC'TnA_GC

Cuts at:    548     548 
   Size:       4370
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1546    2425    2917    3215    1546 
   Size:        879     492     298    2701

  Fragments arranged by size: 

                2701     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    580    4074     580 
   Size:       3494     876
 
BsaAI yAC'GTr

Cuts at:   1723    1723 
   Size:       4370
 
BsaBI GATnn'nnATC

Cuts at:      6    1884    3921       6 
   Size:       1878    2037     455

  Fragments arranged by size: 

                2037    1878     455
 
BsaHI Gr'CG_yC

Cuts at:   1801    2049    2751    4247    1801 
   Size:        248     702    1496    1924

  Fragments arranged by size: 

                1924    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   1559    2080    2758    3032    3179    3590    1559 
   Size:        521     678     274     147     411    2339

  Fragments arranged by size: 

                2339     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    518     802     518 
   Size:        284    4086
 
BseYI C'CCAG_C

Cuts at:   1795    2553    3130    3626    1795 
   Size:        758     577     496    2539

  Fragments arranged by size: 

                2539     758     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    117     117 
   Size:       4370
 
BsiEI CG_ry'CG

Cuts at:    104    1912    1958    3166     104 
   Size:       1808      46    1208    1308

  Fragments arranged by size: 

                1808    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:   1384    2162    2352    3144    1384 
   Size:        778     190     792    2610

  Fragments arranged by size: 

                2610     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    161    1280    1384    1995    2088    2162    2352    2414    3144 
   Size:       1119     104     611      93      74     190      62     730

Cuts at:   3144     161 
   Size:       1387

  Fragments arranged by size: 

                1387    1119     730     611     190     104      93      74
                  62
 
BspEI T'CCGG_A

Cuts at:   3590    3590 
   Size:       4370
 
BspHI T'CATG_A

Cuts at:   3546    3546 
   Size:       4370
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    203    1420    1634    1936    2317     203 
   Size:       1217     214     302     381    2256

  Fragments arranged by size: 

                2256    1217     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2662    2716    4200    4365      50 
   Size:       2612      54    1484     165      55

  Fragments arranged by size: 

                2612    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2282    2282 
   Size:       4370
 
BsrFI r'CCGG_y

Cuts at:    295    1188    2368    2549    2758     295 
   Size:        893    1180     181     209    1907

  Fragments arranged by size: 

                1907    1180     893     209     181
 
BssSI C'ACGA_G

Cuts at:   1008    2641    2999    1008 
   Size:       1633     358    2379

  Fragments arranged by size: 

                2379    1633     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1747    1747 
   Size:       4370
 
BstBI TT'CG_AA

Cuts at:   2731    2731 
   Size:       4370
 
BstYI r'GATC_y

Cuts at:      1     460     986    1395    1862    1885    2220    2466    3478 
   Size:        459     526     409     467      23     335     246    1012

Cuts at:   3478    3564    3576    3922       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1012     526     467     459     449     409     346     335
                 246      86      23      12
 
BstZ17I GTA'TAC

Cuts at:    737     737 
   Size:       4370
 
BtgI C'CryG_G

Cuts at:    107     590     107 
   Size:        483    3887
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2320    2501    2664    2320 
   Size:        181     163    4026

  Fragments arranged by size: 

                4026     181     163
 
BtsI GCAGTG_nn'

Cuts at:    203     795    1074    1613     203 
   Size:        592     279     539    2960

  Fragments arranged by size: 

                2960     592     539     279
 
DraI TTT'AAA

Cuts at:    182    1369     182 
   Size:       1187    3183
 
DrdI GACnn_nn'nnGTC

Cuts at:   2076    2934    2076 
   Size:        858    3512
 
EaeI y'GGCC_r

Cuts at:   1955    2129    2520    2547    3892    1955 
   Size:        174     391      27    1345    2433

  Fragments arranged by size: 

                2433    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1955    1955 
   Size:       4370
 
EarI CTCTTCn'nnn_

Cuts at:   2393    2603    2393 
   Size:        210    4160
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1240    2578    2888    3034    4180    1240 
   Size:       1338     310     146    1146    1430

  Fragments arranged by size: 

                1430    1338    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1382    1382 
   Size:       4370
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    546     559     611    2195    2627    3374    3642     546 
   Size:         13      52    1584     432     747     268    1274

  Fragments arranged by size: 

                1584    1274     747     432     268      52      13
 
EcoO109I rG'GnC_Cy

Cuts at:    327     707     745    1024    1509     327 
   Size:        380      38     279     485    3188

  Fragments arranged by size: 

                3188     485     380     279      38
 
EcoRI G'AATT_C

Cuts at:   1374    1374 
   Size:       4370
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    894    2389     894 
   Size:       1495    2875
 
FspI TGC'GCA

Cuts at:   2151    2151 
   Size:       4370
 
HaeII r_GCGC'y

Cuts at:   1804    2052    3074    4053    1804 
   Size:        248    1022     979    2121

  Fragments arranged by size: 

                2121    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    445     477     642     674    1226    1258    2212    2242    2244 
   Size:         32     165      32     552      32     954      30       2

Cuts at:   2244    2274     445 
   Size:         30    2541

  Fragments arranged by size: 

                2541     954     552     165      32      32      32      30
                  30       2
 
HincII GTy'rAC

Cuts at:   1409    1409 
   Size:       4370
 
KasI G'GCGC_C

Cuts at:   1800    2048    1800 
   Size:        248    4122
 
KpnI G_GTAC'C

Cuts at:   1390    1390 
   Size:       4370
 
MluI A'CGCG_T

Cuts at:   3803    3803 
   Size:       4370
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   3041    3225    3041 
   Size:        184    4186
 
MscI TGG'CCA

Cuts at:   2131    2131 
   Size:       4370
 
MslI CAynn'nnrTG

Cuts at:   2359    2486    2359 
   Size:        127    4243
 
MspA1I CmG'CkG

Cuts at:   1481    1795    2155    3168    3413    1481 
   Size:        314     360    1013     245    2438

  Fragments arranged by size: 

                2438    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   2551    2551 
   Size:       4370
 
NarI GG'CG_CC

Cuts at:   1801    2049    1801 
   Size:        248    4122
 
NcoI C'CATG_G

Cuts at:    107     590     107 
   Size:        483    3887
 
NdeI CA'TA_TG

Cuts at:    351     351 
   Size:       4370
 
NgoMIV G'CCGG_C

Cuts at:   2549    2549 
   Size:       4370
 
NsiI A_TGCA'T

Cuts at:    423     423 
   Size:       4370
 
NspI r_CATG'y

Cuts at:   1423    1923    2830    1423 
   Size:        500     907    2963

  Fragments arranged by size: 

                2963     907     500
 
PasI CC'CwG_GG

Cuts at:    581     860     959     581 
   Size:        279      99    3992

  Fragments arranged by size: 

                3992     279      99
 
PciI A'CATG_T

Cuts at:   2826    2826 
   Size:       4370
 
PmeI GTTT'AAAC

Cuts at:   1369    1369 
   Size:       4370
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3509    3541    3509 
   Size:         32    4338
 
PpuMI rG'GwC_Cy

Cuts at:    327     707     745    1024     327 
   Size:        380      38     279    3673

  Fragments arranged by size: 

                3673     380     279      38
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:    901     933     901 
   Size:         32    4338
 
PstI C_TGCA'G

Cuts at:    744    1417    2102     744 
   Size:        673     685    3012

  Fragments arranged by size: 

                3012     685     673
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4370
 
PvuII CAG'CTG

Cuts at:   1795    2155    1795 
   Size:        360    4010
 
RsrII CG'GwC_CG

Cuts at:   2565    2565 
   Size:       4370
 
SacI G_AGCT'C

Cuts at:   1384    1384 
   Size:       4370
 
SalI G'TCGA_C

Cuts at:   1407    1407 
   Size:       4370
 
SapI GCTCTTCn'nnn_

Cuts at:   2393    2603    2393 
   Size:        210    4160
 
SbfI CC_TGCA'GG

Cuts at:   1417    1417 
   Size:       4370
 
ScaI AGT'ACT

Cuts at:   3887    3887 
   Size:       4370
 
SfcI C'TryA_G

Cuts at:     33     740    1413    2098    3091    3282      33 
   Size:        707     673     685     993     191    1121

  Fragments arranged by size: 

                1121     993     707     685     673     191
 
SfoI GGC'GCC

Cuts at:   1802    2050    1802 
   Size:        248    4122
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4370
 
SmaI CCC'GGG

Cuts at:    448    1392     448 
   Size:        944    3426
 
SmlI C'TyrA_G

Cuts at:   1525    2440    2932    3194    1525 
   Size:        915     492     262    2701

  Fragments arranged by size: 

                2701     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4370
 
SphI G_CATG'C

Cuts at:   1423    1923    1423 
   Size:        500    3870
 
StyI C'CwwG_G

Cuts at:    107     590    1504    1858     107 
   Size:        483     914     354    2619

  Fragments arranged by size: 

                2619     914     483     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    712    1064    1953    2086    2545    2754     712 
   Size:        352     889     133     459     209    2328

  Fragments arranged by size: 

                2328     889     459     352     209     133
 
TatI w'GTAC_w

Cuts at:   3885    3885 
   Size:       4370
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3887    3919    3887 
   Size:         32    4338
 
Tth111I GACn'n_nGTC

Cuts at:    796    1075    2167     796 
   Size:        279    1092    2999

  Fragments arranged by size: 

                2999    1092     279
 
XbaI T'CTAG_A

Cuts at:   1401    1401 
   Size:       4370
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    588    1865     588 
   Size:       1277    3093
 
XmaI C'CCGG_G

Cuts at:    446    1390     446 
   Size:        944    3426
 
XmnI GAAnn'nnTTC

Cuts at:   1220    1220 
   Size:       4370
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I     BmrI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstYI
  BstZ17I     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI
    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NsiI
     NspI     PasI     PciI     PmeI     PpiI    PpuMI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI
     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI     BbsI    BbvCI     BcgI     BglI
    BmgBI     BmtI    BsaXI    BsiWI     BsmI    BsmBI    BsrGI   BssHII
   BstEII    BstXI   Bsu36I     ClaI    CspCI   DraIII    EcoNI    EcoRV
     FseI    FspAI  HindIII     HpaI     MfeI     NheI     NotI     NruI
     PacI    PflMI     PfoI     PmlI    PshAI     PsiI   PspOMI    PspXI
    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI
     StuI     SwaI     XhoI     ZraI
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