Restriction map for pF1KB5772
 (Linear) (Six-base) MAPSORT of: KIBB5772.seq  Check: 5234  from: 1  to: 2286



KIBB5772.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:05  ..

AccI GT'mk_AC

Cuts at:      0     633    1493    1577    2286 
   Size:        633     860      84     709

  Fragments arranged by size: 

                 860     709     633      84
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     265     880     893    1136    1330    1869    1877    2113 
   Size:        265     615      13     243     194     539       8     236

Cuts at:   2113    2286 
   Size:        173

  Fragments arranged by size: 

                 615     539     265     243     236     194     173      13
                   8
 
AlwNI CAG_nnn'CTG

Cuts at:      0     504    2286 
   Size:        504    1782
 
ApoI r'AATT_y

Cuts at:      0     421     864    1660    1910    2286 
   Size:        421     443     796     250     376

  Fragments arranged by size: 

                 796     443     421     376     250
 
BamHI G'GATC_C

Cuts at:      0     516    1508    2286 
   Size:        516     992     778

  Fragments arranged by size: 

                 992     778     516
 
BanII G_rGCy'C

Cuts at:      0     215    1613    1928    2195    2286 
   Size:        215    1398     315     267      91

  Fragments arranged by size: 

                1398     315     267     215      91
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     328    2286 
   Size:        328    1958
 
BbvCI CC'TCA_GC

Cuts at:      0     521    1757    2286 
   Size:        521    1236     529

  Fragments arranged by size: 

                1236     529     521
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     861    2286 
   Size:        861    1425
 
BclI T'GATC_A

Cuts at:      0    1020    2286 
   Size:       1020    1266
 
BfrBI ATG'CAT

Cuts at:      0     548    2286 
   Size:        548    1738
 
BlpI GC'TnA_GC

Cuts at:      0     380    2286 
   Size:        380    1906
 
Bme1580I G_kGCm'C

Cuts at:      0     768    2286 
   Size:        768    1518
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1384    1915    2286 
   Size:       1384     531     371

  Fragments arranged by size: 

                1384     531     371
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1542    2286 
   Size:       1542     744
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     118     199     275     441    1609    1635    1823    2237 
   Size:        118      81      76     166    1168      26     188     414

Cuts at:   2237    2286 
   Size:         49

  Fragments arranged by size: 

                1168     414     188     166     118      81      76      49
                  26
 
Bpu10I CC'TnA_GC

Cuts at:      0     521    1599    1757    2286 
   Size:        521    1078     158     529

  Fragments arranged by size: 

                1078     529     521     158
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     328    1378    2179    2286 
   Size:        328    1050     801     107

  Fragments arranged by size: 

                1050     801     328     107
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     342    2286 
   Size:        342    1944
 
BsaWI w'CCGG_w

Cuts at:      0     317    2286 
   Size:        317    1969
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      91     121     127     157     187    2286 
   Size:         91      30       6      30      30    2099

  Fragments arranged by size: 

                2099      91      30      30      30       6
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     126     223     813    1531    2286 
   Size:        126      97     590     718     755

  Fragments arranged by size: 

                 755     718     590     126      97
 
BseYI C'CCAG_C

Cuts at:      0    2082    2270    2286 
   Size:       2082     188      16

  Fragments arranged by size: 

                2082     188      16
 
BsiHKAI G_wGCw'C

Cuts at:      0     215    1009    1528    1613    1928    2195    2286 
   Size:        215     794     519      85     315     267      91

  Fragments arranged by size: 

                 794     519     315     267     215      91      85
 
BsmI GAATG_Cn'

Cuts at:      0     550    2252    2286 
   Size:        550    1702      34

  Fragments arranged by size: 

                1702     550      34
 
Bsp1286I G_dGCh'C

Cuts at:      0     215     768    1009    1528    1613    1928    2195    2286 
   Size:        215     553     241     519      85     315     267      91

  Fragments arranged by size: 

                 553     519     315     267     241     215      91      85
            
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1706    2286 
   Size:       1706     580
 
BsrBI CCG'CTC

Cuts at:      0     138    2286 
   Size:        138    2148
 
BsrDI GCAATG_nn'

Cuts at:      0     191    2286 
   Size:        191    2095
 
BsrGI T'GTAC_A

Cuts at:      0     718    2286 
   Size:        718    1568
 
BstEII G'GTnAC_C

Cuts at:      0    1762    2286 
   Size:       1762     524
 
BstYI r'GATC_y

Cuts at:      0     128     516    1508    2286 
   Size:        128     388     992     778

  Fragments arranged by size: 

                 992     778     388     128
 
BstZ17I GTA'TAC

Cuts at:      0    1578    2286 
   Size:       1578     708
 
Bsu36I CC'TnA_GG

Cuts at:      0    2036    2286 
   Size:       2036     250
 
BtsI GCAGTG_nn'

Cuts at:      0    1042    2286 
   Size:       1042    1244
 
EaeI y'GGCC_r

Cuts at:      0     188    1322    2286 
   Size:        188    1134     964

  Fragments arranged by size: 

                1134     964     188
 
EarI CTCTTCn'nnn_

Cuts at:      0    1178    1389    1498    2286 
   Size:       1178     211     109     788

  Fragments arranged by size: 

                1178     788     211     109
 
EcoICRI GAG'CTC

Cuts at:      0     213    1611    1926    2193    2286 
   Size:        213    1398     315     267      93

  Fragments arranged by size: 

                1398     315     267     213      93
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     118     199     265     275     441     880     893    1136 
   Size:        118      81      66      10     166     439      13     243

Cuts at:   1136    1330    1609    1635    1823    1869    1877    2113    2237 
   Size:        194     279      26     188      46       8     236     124

Cuts at:   2237    2286 
   Size:         49

  Fragments arranged by size: 

                 439     279     243     236     194     188     166     124
                 118      81      66      49      46      26      13      10
                   8
 
EcoO109I rG'GnC_Cy

Cuts at:      0    2200    2286 
   Size:       2200      86
 
EcoRI G'AATT_C

Cuts at:      0    1910    2286 
   Size:       1910     376
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     322    2286 
   Size:        322    1964
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      34      66     157     189     549     581     920     952 
   Size:         34      32      91      32     360      32     339      32

Cuts at:    952    1081    1113    1885    1917    2248    2280    2286 
   Size:        129      32     772      32     331      32       6

  Fragments arranged by size: 

                 772     360     339     331     129      91      34      32
                  32      32      32      32      32      32       6
 
HincII GTy'rAC

Cuts at:      0     634    1470    1779    1959    2286 
   Size:        634     836     309     180     327

  Fragments arranged by size: 

                 836     634     327     309     180
 
MfeI C'AATT_G

Cuts at:      0     714    2286 
   Size:        714    1572
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2095    2286 
   Size:       2095     191
 
MscI TGG'CCA

Cuts at:      0     190    1324    2286 
   Size:        190    1134     962

  Fragments arranged by size: 

                1134     962     190
 
MslI CAynn'nnrTG

Cuts at:      0      41     676    2286 
   Size:         41     635    1610

  Fragments arranged by size: 

                1610     635      41
 
MspA1I CmG'CkG

Cuts at:      0     171     234    1032    1308    2286 
   Size:        171      63     798     276     978

  Fragments arranged by size: 

                 978     798     276     171      63
 
NdeI CA'TA_TG

Cuts at:      0     260    2286 
   Size:        260    2026
 
NsiI A_TGCA'T

Cuts at:      0     550    2286 
   Size:        550    1736
 
NspI r_CATG'y

Cuts at:      0     552    1136    1249    1492    1584    2286 
   Size:        552     584     113     243      92     702

  Fragments arranged by size: 

                 702     584     552     243     113      92
 
PfoI T'CCnGG_A

Cuts at:      0     131    2286 
   Size:        131    2155
 
PpuMI rG'GwC_Cy

Cuts at:      0    2200    2286 
   Size:       2200      86
 
PstI C_TGCA'G

Cuts at:      0     248    1049    2286 
   Size:        248     801    1237

  Fragments arranged by size: 

                1237     801     248
 
PvuII CAG'CTG

Cuts at:      0     171     234    1032    1308    2286 
   Size:        171      63     798     276     978

  Fragments arranged by size: 

                 978     798     276     171      63
 
SacI G_AGCT'C

Cuts at:      0     215    1613    1928    2195    2286 
   Size:        215    1398     315     267      91

  Fragments arranged by size: 

                1398     315     267     215      91
 
SalI G'TCGA_C

Cuts at:      0     632    2286 
   Size:        632    1654
 
SanDI GG'GwC_CC

Cuts at:      0    2200    2286 
   Size:       2200      86
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1178    2286 
   Size:       1178    1108
 
SfcI C'TryA_G

Cuts at:      0     244    1045    1719    2286 
   Size:        244     801     674     567

  Fragments arranged by size: 

                 801     674     567     244
 
SmlI C'TyrA_G

Cuts at:      0     307    1357    2194    2286 
   Size:        307    1050     837      92

  Fragments arranged by size: 

                1050     837     307      92
 
SphI G_CATG'C

Cuts at:      0    1136    2286 
   Size:       1136    1150
 
StuI AGG'CCT

Cuts at:      0     286    1594    2286 
   Size:        286    1308     692

  Fragments arranged by size: 

                1308     692     286
 
StyI C'CwwG_G

Cuts at:      0     648    2286 
   Size:        648    1638
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      53    2286 
   Size:         53    2233
 
TatI w'GTAC_w

Cuts at:      0     718    2286 
   Size:        718    1568
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1815    1847    1901    1933    2286 
   Size:       1815      32      54      32     353

  Fragments arranged by size: 

                1815     353      54      32      32
 
XbaI T'CTAG_A

Cuts at:      0    1749    2286 
   Size:       1749     537
 
XmnI GAAnn'nnTTC

Cuts at:      0       9    1301    1862    2286 
   Size:          9    1292     561     424

  Fragments arranged by size: 

                1292     561     424       9
 

 Enzymes that do cut:

     AccI     AcuI    AlwNI     ApoI    BamHI    BanII     BbsI    BbvCI
    BciVI     BclI    BfrBI     BlpI Bme1580I     BmrI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaWI    BsaXI    BseRI    BseYI  BsiHKAI
     BsmI Bsp1286I    BspMI    BsrBI    BsrDI    BsrGI   BstEII    BstYI
  BstZ17I   Bsu36I     BtsI     EaeI     EarI  EcoICRI  Eco57MI EcoO109I
    EcoRI     FalI    Hin4I   HincII     MfeI     MmeI     MscI     MslI
   MspA1I     NdeI     NsiI     NspI     PfoI    PpuMI     PstI    PvuII
     SacI     SalI    SanDI     SapI     SfcI     SmlI     SphI     StuI
     StyI    TaqII     TatI     TstI     XbaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI     BanI     BbeI     BcgI     BglI
    BglII    BmgBI     BmtI    BsaAI    BsaBI    BsaHI     BsgI    BsiEI
    BsiWI    BsmBI    BspEI    BspHI    BsrFI   BssHII    BssSI   BstAPI
    BstBI    BstXI     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EciI    EcoNI    EcoRV     FseI     FspI    FspAI
    HaeII  HindIII     HpaI     KasI     KpnI     MluI     NaeI     NarI
     NcoI   NgoMIV     NheI     NotI     NruI     PacI     PasI     PciI
    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII    SacII     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI     SspI
     SwaI  Tth111I     XcmI     XhoI     XmaI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com