Restriction map for pF1KB6582
##################################
# NO CUT ENZYMES (  131 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AflIII      AgeI        Alw44I      AocI        
Aor51HI     ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspHI       AviII       AvrII       
BalI        BamHI       BanI        BbeI        BbrPI       
BbsI        BcgI        BciVI       BfrI        BglI        
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaWI       BseAI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmBI       BspDI       BspEI       BspLU11I    
BspMI       BsrDI       BsrFI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstXI       BstZ17I     
Bsu36I      CelII       Cfr10I      ClaI        DraI        
DraIII      DrdI        EaeI        EagI        EclXI       
Eco47III    Eco52I      Eco81I      EcoRV       EcoT22I     
FseI        FspI        HaeII       HincII      HindII      
HpaI        KasI        KpnI        KspI        MaeII       
MfeI        MluI        MluNI       MroI        MscI        
MslI        MunI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        SacI        SacII       
SalI        SapI        SbfI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspI        StuI        
SwaI        XbaI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AcsI           91  GAATTC               [GA]^AATT[CT]
AcyI          217  GACGCC               G[GA]^CG[CT]C
AhdI          229  GACAGCGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI         169  CAGAGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI         1114  GGGCCC               GGGCC^C
ApoI           91  GAATTC               [GA]^AATT[CT]
AspEI         229  GACAGCGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbvCI        1046  CCTCAGC              CCTCAGC(-5/-2)
BclI          429  TGATCA               T^GATCA
BglII         839  AGATCT               A^GATCT
BpmI         1182  CTCCAG               CTGGAG(16/14)
BsaBI         337  GATACAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         217  GACGCC               G[GA]^CG[CT]C
BsaI          307  GAGACC               GGTCTC(1/5)
BseRI         241  GAGGAG               GAGGAG(10/8)
BsmI         1096  GCATTC               GAATGC(1/-1)
BspHI        1130  TCATGA               T^CATGA
BsrBI         582  CCGCTC               CCGCTC(-3/-3)
BstEII       1023  GGTCACC              G^GT[ACGT]ACC
BstPI        1023  GGTCACC              G^GT[ACGT]ACC
CpoI          203  CGGACCG              CG^G[AT]CCG
DsaI          606  CCGTGG               C^C[GA][CT]GG
Eam1105I      229  GACAGCGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI         117  CCTGCGCGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      1023  GGTCACC              G^GT[ACGT]ACC
EcoRI          91  GAATTC               G^AATTC
FbaI          429  TGATCA               T^GATCA
HgaI          217  GACGC                GACGC(5/10)
Hin1I         217  GACGCC               G[GA]^CG[CT]C
HindIII       395  AAGCTT               A^AGCTT
MamI          337  GATACAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NspI          492  GCATGT               [GA]CATG^[CT]
PflMI         168  CCAGAGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI           40  CTGCAG               CTGCA^G
PvuII        1162  CAGCTG               CAG^CTG
RcaI         1130  TCATGA               T^CATGA
RsrII         203  CGGACCG              CG^G[AT]CCG
SfcI           40  CTGCAG               C^T[GA][CT]AG
SmlI          446  CTCAAG               C^T[CT][GA]AG
Tsp509I        92  AATT                 ^AATT
TspRI          98  CAGTG                CA[GC]TG[ACGT][ACGT]^
Van91I        168  CCAGAGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AccII         121  CGCG                 CG^CG
              130  CGCG                
AspI           82  GACCTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              205  GACCGTGTC           
AvaI          645  CCCGAG               C^[CT]CG[GA]G
             1204  CTCGGG              
AvaII         204  GGACC                G^G[AT]CC
              696  GGACC               
BanII          25  GGGCTC               G[GA]GC[CT]^C
             1114  GGGCCC              
BsmAI         307  GAGAC                GTCTC(1/5)
              518  GAGAC               
BsmFI         227  GGGAC                GGGAC(10/14)
              690  GGGAC               
BsoBI         645  CCCGAG               C^[CT]CG[GA]G
             1204  CTCGGG              
Bsp1407I      635  TGTACA               T^GTACA
             1089  TGTACA              
BsrGI         635  TGTACA               T^GTACA
             1089  TGTACA              
BsrI          693  ACTGG                ACTGG(1/-1)
              265  CCAGT               
BstUI         121  CGCG                 CG^CG
              130  CGCG                
CfoI          120  GCGC                 GCG^C
              131  GCGC                
DraII        1113  GGGGCCC              [GA]G^G[ACGT]CC[CT]
             1114  GGGCCCT             
EcoO109I     1113  GGGGCCC              [GA]G^G[ACGT]CC[CT]
             1114  GGGCCCT             
EcoT14I       346  CCAAGG               C^C[AT][AT]GG
              504  CCAAGG              
HhaI          120  GCGC                 GCG^C
              131  GCGC                
HinP1I        120  GCGC                 G^CGC
              131  GCGC                
MseI          281  TTAA                 T^TAA
              785  TTAA                
MspA1I        984  CCGCTG               C[AC]G^C[GT]G
             1162  CAGCTG              
MvnI          121  CGCG                 CG^CG
              130  CGCG                
PflFI          82  GACCTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              205  GACCGTGTC           
ScaI          104  AGTACT               AGT^ACT
             1001  AGTACT              
SspBI         635  TGTACA               T^GTACA
             1089  TGTACA              
StyI          346  CCAAGG               C^C[AT][AT]GG
              504  CCAAGG              
Tru9I         281  TTAA                 T^TAA
              785  TTAA                
Tsp45I        703  GTGAC                ^GT[GC]AC
             1024  GTCAC               
Tth111I        82  GACCTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              205  GACCGTGTC           
XcmI          504  CCAAGGAGATGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1184  CCAGCGTGGATCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   56 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        BbvI        
BcnI        BfaI        BmyI        BsaJI       BsiYI       
BslI        Bsp1286I    BssKI       BstF5I      BstNI       
BstYI       Cac8I       Cfr13I      DdeI        DpnII       
EarI        EcoRII      FauI        Fnu4HI      FokI        
HaeIII      HapII       HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeIII      MboI        
MboII       MflI        MnlI        MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PleI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       TaqI        TfiI        TseI        TthHB8I     
XhoII       
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