Restriction map for pF1KB5408
 (Linear) (Six-base) MAPSORT of: KIBB5408.seq  Check: 9487  from: 1  to: 1251



KIBB5408.orf

 With 182 enzymes: SgfI * 

                        September 18, 2009 17:00  ..

AccI GT'mk_AC

Cuts at:      0      62    1251 
   Size:         62    1189
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      40    1251 
   Size:         40    1211
 
AflII C'TTAA_G

Cuts at:      0     649    1251 
   Size:        649     602
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      94    1251 
   Size:         94    1157
 
AlwNI CAG_nnn'CTG

Cuts at:      0     276     961    1251 
   Size:        276     685     290

  Fragments arranged by size: 

                 685     290     276
 
ApaLI G'TGCA_C

Cuts at:      0     133    1251 
   Size:        133    1118
 
ApoI r'AATT_y

Cuts at:      0     426     803    1251 
   Size:        426     377     448

  Fragments arranged by size: 

                 448     426     377
 
AseI AT'TA_AT

Cuts at:      0     158    1251 
   Size:        158    1093
 
AvrII C'CTAG_G

Cuts at:      0     942    1251 
   Size:        942     309
 
BanI G'GyrC_C

Cuts at:      0       8      15     103     546     934    1251 
   Size:          8       7      88     443     388     317

  Fragments arranged by size: 

                 443     388     317      88       8       7
 
BanII G_rGCy'C

Cuts at:      0      36    1082    1251 
   Size:         36    1046     169

  Fragments arranged by size: 

                1046     169      36
 
BbeI G_GCGC'C

Cuts at:      0      12      19     107    1251 
   Size:         12       7      88    1144

  Fragments arranged by size: 

                1144      88      12       7
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     605    1251 
   Size:        605     646
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     325     359    1251 
   Size:        325      34     892

  Fragments arranged by size: 

                 892     325      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1155    1251 
   Size:       1155      96
 
Bme1580I G_kGCm'C

Cuts at:      0     137     551     955    1251 
   Size:        137     414     404     296

  Fragments arranged by size: 

                 414     404     296     137
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      83    1251 
   Size:         83    1168
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     533     577     589     979    1210    1251 
   Size:        533      44      12     390     231      41

  Fragments arranged by size: 

                 533     390     231      44      41      12
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     189    1251 
   Size:        189    1062
 
BsaAI yAC'GTr

Cuts at:      0    1011    1251 
   Size:       1011     240
 
BsaHI Gr'CG_yC

Cuts at:      0       9      16     104    1251 
   Size:          9       7      88    1147

  Fragments arranged by size: 

                1147      88       9       7
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     439     469    1251 
   Size:        439      30     782

  Fragments arranged by size: 

                 782     439      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     113    1251 
   Size:        113    1138
 
BseYI C'CCAG_C

Cuts at:      0      67     366     395     550    1251 
   Size:         67     299      29     155     701

  Fragments arranged by size: 

                 701     299     155      67      29
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     169     460     676    1251 
   Size:        169     291     216     575

  Fragments arranged by size: 

                 575     291     216     169
 
BsiHKAI G_wGCw'C

Cuts at:      0     137    1082    1251 
   Size:        137     945     169

  Fragments arranged by size: 

                 945     169     137
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     912    1251 
   Size:        912     339
 
Bsp1286I G_dGCh'C

Cuts at:      0      36     137     551     955    1082    1251 
   Size:         36     101     414     404     127     169

  Fragments arranged by size: 

                 414     404     169     127     101      36
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     740     750    1251 
   Size:        740      10     501

  Fragments arranged by size: 

                 740     501      10
 
BsrBI CCG'CTC

Cuts at:      0     388    1251 
   Size:        388     863
 
BsrFI r'CCGG_y

Cuts at:      0       5     322    1251 
   Size:          5     317     929

  Fragments arranged by size: 

                 929     317       5
 
BssSI C'ACGA_G

Cuts at:      0     400    1251 
   Size:        400     851
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     141     438    1146    1251 
   Size:        141     297     708     105

  Fragments arranged by size: 

                 708     297     141     105
 
BstEII G'GTnAC_C

Cuts at:      0     630    1251 
   Size:        630     621
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     222     778    1251 
   Size:        222     556     473

  Fragments arranged by size: 

                 556     473     222
 
BtgI C'CryG_G

Cuts at:      0     635    1023    1251 
   Size:        635     388     228

  Fragments arranged by size: 

                 635     388     228
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      69    1251 
   Size:         69    1182
 
BtsI GCAGTG_nn'

Cuts at:      0     463    1251 
   Size:        463     788
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     873     908    1251 
   Size:        873      35     343

  Fragments arranged by size: 

                 873     343      35
 
EaeI y'GGCC_r

Cuts at:      0     894    1251 
   Size:        894     357
 
EarI CTCTTCn'nnn_

Cuts at:      0     796    1251 
   Size:        796     455
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     110    1173    1251 
   Size:        110    1063      78

  Fragments arranged by size: 

                1063     110      78
 
EcoICRI GAG'CTC

Cuts at:      0    1080    1251 
   Size:       1080     171
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      40     533     577     589     979    1210    1251 
   Size:         40     493      44      12     390     231      41

  Fragments arranged by size: 

                 493     390     231      44      41      40      12
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     559    1251 
   Size:        559     692
 
EcoO109I rG'GnC_Cy

Cuts at:      0     340     613     645    1088    1251 
   Size:        340     273      32     443     163

  Fragments arranged by size: 

                 443     340     273     163      32
 
HaeII r_GCGC'y

Cuts at:      0      12      19     107     168     819    1251 
   Size:         12       7      88      61     651     432

  Fragments arranged by size: 

                 651     432      88      61      12       7
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     439     471    1251 
   Size:        439      32     780

  Fragments arranged by size: 

                 780     439      32
 
HincII GTy'rAC

Cuts at:      0      63    1251 
   Size:         63    1188
 
KasI G'GCGC_C

Cuts at:      0       8      15     103    1251 
   Size:          8       7      88    1148

  Fragments arranged by size: 

                1148      88       8       7
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     887    1251 
   Size:        887     364
 
MscI TGG'CCA

Cuts at:      0     896    1251 
   Size:        896     355
 
MslI CAynn'nnrTG

Cuts at:      0     191    1033    1251 
   Size:        191     842     218

  Fragments arranged by size: 

                 842     218     191
 
MspA1I CmG'CkG

Cuts at:      0     678     690    1137    1251 
   Size:        678      12     447     114

  Fragments arranged by size: 

                 678     447     114      12
 
NaeI GCC'GGC

Cuts at:      0       7    1251 
   Size:          7    1244
 
NarI GG'CG_CC

Cuts at:      0       9      16     104    1251 
   Size:          9       7      88    1147

  Fragments arranged by size: 

                1147      88       9       7
 
NcoI C'CATG_G

Cuts at:      0    1023    1251 
   Size:       1023     228
 
NgoMIV G'CCGG_C

Cuts at:      0       5    1251 
   Size:          5    1246
 
PpuMI rG'GwC_Cy

Cuts at:      0     645    1088    1251 
   Size:        645     443     163

  Fragments arranged by size: 

                 645     443     163
 
PstI C_TGCA'G

Cuts at:      0     146     252     470     751    1251 
   Size:        146     106     218     281     500

  Fragments arranged by size: 

                 500     281     218     146     106
 
PvuII CAG'CTG

Cuts at:      0     678     690    1137    1251 
   Size:        678      12     447     114

  Fragments arranged by size: 

                 678     447     114      12
 
SacI G_AGCT'C

Cuts at:      0    1082    1251 
   Size:       1082     169
 
SalI G'TCGA_C

Cuts at:      0      61    1251 
   Size:         61    1190
 
SanDI GG'GwC_CC

Cuts at:      0    1088    1251 
   Size:       1088     163
 
SbfI CC_TGCA'GG

Cuts at:      0     751    1251 
   Size:        751     500
 
SfcI C'TryA_G

Cuts at:      0     142     248     466     747    1251 
   Size:        142     106     218     281     504

  Fragments arranged by size: 

                 504     281     218     142     106
 
SfoI GGC'GCC

Cuts at:      0      10      17     105    1251 
   Size:         10       7      88    1146

  Fragments arranged by size: 

                1146      88      10       7
 
SmlI C'TyrA_G

Cuts at:      0     168     649    1251 
   Size:        168     481     602

  Fragments arranged by size: 

                 602     481     168
 
StuI AGG'CCT

Cuts at:      0     655     745     947    1251 
   Size:        655      90     202     304

  Fragments arranged by size: 

                 655     304     202      90
 
StyI C'CwwG_G

Cuts at:      0     329     942    1023    1083    1251 
   Size:        329     613      81      60     168

  Fragments arranged by size: 

                 613     329     168      81      60
 
Tth111I GACn'n_nGTC

Cuts at:      0     885    1251 
   Size:        885     366
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     219    1251 
   Size:        219    1032
 

 Enzymes that do cut:

     AccI     AcuI    AflII     AlfI    AlwNI    ApaLI     ApoI     AseI
    AvrII     BanI    BanII     BbeI     BbsI     BcgI     BglI Bme1580I
     BmrI     BpmI    BpuEI    BsaAI    BsaHI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrBI    BsrFI    BssSI
   BstAPI   BstEII    BstXI     BtgI    BtgZI     BtsI    CspCI     EaeI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    HaeII    Hin4I
   HincII     KasI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI   NgoMIV    PpuMI     PstI    PvuII     SacI     SalI    SanDI
     SbfI     SfcI     SfoI     SmlI     StuI     StyI  Tth111I     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI   AflIII     AgeI     AhdI
     AleI     AloI     ApaI     AscI    AsiSI     AvaI     BaeI    BamHI
    BbvCI    BciVI     BclI    BfrBI    BglII     BlpI    BmgBI     BmtI
     BplI   Bpu10I     BsaI    BsaBI    BsaWI    BsiEI    BsiWI     BsmI
    BspEI    BspHI    BsrDI    BsrGI   BssHII    BstBI    BstYI  BstZ17I
   Bsu36I     ClaI     DraI   DraIII     DrdI     EagI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI  HindIII     HpaI     KpnI     MfeI
     MluI     NdeI     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII     SapI
     ScaI    SexAI     SfiI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI    TaqII     TatI     TstI     XbaI
     XhoI     XmaI     XmnI     ZraI
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