Restriction map for pF1KB7064
 (Circular) (Six-base) MAPSORT of: pF1KB7064.seq  Check: 985  from: 1  to: 5135



pF1KB7064  5135 bp

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

AccI GT'mk_AC

Cuts at:   2173    2173 
   Size:       5135
 
Acc65I G'GTAC_C

Cuts at:   2151    2151 
   Size:       5135
 
AclI AA'CG_TT

Cuts at:   4975    4975 
   Size:       5135
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    365     377     443     635     848    1144    1527    2960    3392 
   Size:         12      66     192     213     296     383    1433     432

Cuts at:   3392    4139    4407     365 
   Size:        747     268    1093

  Fragments arranged by size: 

                1433    1093     747     432     383     296     268     213
                 192      66      12
 
AflIII A'CryG_T

Cuts at:   2487    3591    4568    2487 
   Size:       1104     977    3054

  Fragments arranged by size: 

                3054    1104     977
 
AgeI A'CCGG_T

Cuts at:   3523    3523 
   Size:       5135
 
AhdI GACnn_n'nnGTC

Cuts at:    879     879 
   Size:       5135
 
AleI CACnn'nnGTG

Cuts at:    766     766 
   Size:       5135
 
AlwNI CAG_nnn'CTG

Cuts at:    884    1550    1952    2101    2440    4007     884 
   Size:        666     402     149     339    1567    2012

  Fragments arranged by size: 

                2012    1567     666     402     339     149
 
ApaI G_GGCC'C

Cuts at:   1539    1849    1539 
   Size:        310    4825
 
ApaLI G'TGCA_C

Cuts at:   3905    3905 
   Size:       5135
 
ApoI r'AATT_y

Cuts at:   2139    4488    4744    2139 
   Size:       2349     256    2530

  Fragments arranged by size: 

                2530    2349     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       5135
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5135
 
AvaI C'yCGr_G

Cuts at:   1865    1940    2155    1865 
   Size:         75     215    4845

  Fragments arranged by size: 

                4845     215      75
 
AvrII C'CTAG_G

Cuts at:   1681    1681 
   Size:       5135
 
BamHI G'GATC_C

Cuts at:    748    2160     748 
   Size:       1412    3723
 
BanI G'GyrC_C

Cuts at:   1016    2151    2565    2813    2848    1016 
   Size:       1135     414     248      35    3303

  Fragments arranged by size: 

                3303    1135     414     248      35
 
BanII G_rGCy'C

Cuts at:    239    1539    1619    1677    1849    1921    2149    3179     239 
   Size:       1300      80      58     172      72     228    1030    2195

  Fragments arranged by size: 

                2195    1300    1030     228     172      80      72      58
            
 
BbeI G_GCGC'C

Cuts at:   2569    2817    2569 
   Size:        248    4887
 
BbsI GAAGACnn'nnnn_

Cuts at:    365     847    1251     365 
   Size:        482     404    4249

  Fragments arranged by size: 

                4249     482     404
 
BbvCI CC'TCA_GC

Cuts at:    193     978    1226    1595     193 
   Size:        785     248     369    3733

  Fragments arranged by size: 

                3733     785     369     248
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1846    1863    1880    1897    1846 
   Size:         17      17      17    5084

  Fragments arranged by size: 

                5084      17      17      17
 
BciVI GTATCCnnnnn_n'

Cuts at:   3033    3794    5135    3033 
   Size:        761    1341    3033

  Fragments arranged by size: 

                3033    1341     761
 
BclI T'GATC_A

Cuts at:   2655    2655 
   Size:       5135
 
BglI GCCn_nnn'nGGC

Cuts at:    218     218 
   Size:       5135
 
BglII A'GATC_T

Cuts at:   2650    4687    2650 
   Size:       2037    3098
 
BlpI GC'TnA_GC

Cuts at:   1613    1915    2247    1613 
   Size:        302     332    4501

  Fragments arranged by size: 

                4501     332     302
 
Bme1580I G_kGCm'C

Cuts at:   1539    1849    1959    2760    2853    3909    1539 
   Size:        310     110     801      93    1056    2765

  Fragments arranged by size: 

                2765    1056     801     310     110      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    172     403     953    1019    1611    2029    2347    2428    2763 
   Size:        231     550      66     592     418     318      81     335

Cuts at:   2763    4907     172 
   Size:       2144     400

  Fragments arranged by size: 

                2144     592     550     418     400     335     318     231
                  81      66
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1251    3502    1251 
   Size:       2251    2884
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    225     307     395     728    1670     225 
   Size:         82      88     333     942    3690

  Fragments arranged by size: 

                3690     942     333      88      82
 
Bpu10I CC'TnA_GC

Cuts at:    193     978    1226    1595     193 
   Size:        785     248     369    3733

  Fragments arranged by size: 

                3733     785     369     248
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    988    1696    2073    2311    3190    3682    3980     988 
   Size:        708     377     238     879     492     298    2143

  Fragments arranged by size: 

                2143     879     708     492     377     298     238
 
BsaI GGTCTCn'nnnn_

Cuts at:   4839    4839 
   Size:       5135
 
BsaAI yAC'GTr

Cuts at:   2488    2488 
   Size:       5135
 
BsaBI GATnn'nnATC

Cuts at:      6    2649    4686       6 
   Size:       2643    2037     455

  Fragments arranged by size: 

                2643    2037     455
 
BsaHI Gr'CG_yC

Cuts at:    339     907    2566    2814    3516    5012     339 
   Size:        568    1659     248     702    1496     462

  Fragments arranged by size: 

                1659    1496     702     568     462     248
 
BsaWI w'CCGG_w

Cuts at:   1740    2324    2845    3523    3797    3944    4355    1740 
   Size:        584     521     678     274     147     411    2520

  Fragments arranged by size: 

                2520     678     584     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    181     296     364     601     634     694     697     928    1051 
   Size:        115      68     237      33      60       3     231     123

Cuts at:   1051    1315    1318    1444    1447    1667    1924    2007     181 
   Size:        264       3     126       3     220     257      83    3309

  Fragments arranged by size: 

                3309     264     257     237     231     220     126     123
                 115      83      68      60      33       3       3       3
            
 
BseYI C'CCAG_C

Cuts at:    538     757     984     998    1550    2560    3318    3895    4391 
   Size:        219     227      14     552    1010     758     577     496

Cuts at:   4391     538 
   Size:       1282

  Fragments arranged by size: 

                1282    1010     758     577     552     496     227     219
                  14
 
BsiEI CG_ry'CG

Cuts at:    104    2677    2723    3931     104 
   Size:       2573      46    1208    1308

  Fragments arranged by size: 

                2573    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    528    2149    2927    3117    3909     528 
   Size:       1621     778     190     792    1754

  Fragments arranged by size: 

                1754    1621     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    239     528    1539    1619    1677    1849    1921    1959    2149 
   Size:        289    1011      80      58     172      72      38     190

Cuts at:   2149    2760    2853    2927    3117    3179    3909     239 
   Size:        611      93      74     190      62     730    1465

  Fragments arranged by size: 

                1465    1011     730     611     289     190     190     172
                  93      80      74      72      62      58      38
 
BspEI T'CCGG_A

Cuts at:   1740    4355    1740 
   Size:       2615    2520
 
BspHI T'CATG_A

Cuts at:   1734    4311    1734 
   Size:       2577    2558
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   2185    2399    2701    3082    2185 
   Size:        214     302     381    4238

  Fragments arranged by size: 

                4238     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2081    3427    3481    4965    5130      50 
   Size:       2031    1346      54    1484     165      55

  Fragments arranged by size: 

                2031    1484    1346     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   3047    3047 
   Size:       5135
 
BsrFI r'CCGG_y

Cuts at:   1824    3133    3314    3523    1824 
   Size:       1309     181     209    3436

  Fragments arranged by size: 

                3436    1309     209     181
 
BssSI C'ACGA_G

Cuts at:   3406    3764    3406 
   Size:        358    4777
 
BstAPI GCAn_nnn'nTGC

Cuts at:   2101    2512    2101 
   Size:        411    4724
 
BstBI TT'CG_AA

Cuts at:   3496    3496 
   Size:       5135
 
BstYI r'GATC_y

Cuts at:      1     748    2160    2627    2650    2985    3231    4243    4329 
   Size:        747    1412     467      23     335     246    1012      86

Cuts at:   4329    4341    4687       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1412    1012     747     467     449     346     335     246
                  86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:    214    1963     214 
   Size:       1749    3386
 
BtgI C'CryG_G

Cuts at:    107     142     674     800     107 
   Size:         35     532     126    4442

  Fragments arranged by size: 

                4442     532     126      35
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   3085    3266    3429    3085 
   Size:        181     163    4791

  Fragments arranged by size: 

                4791     181     163
 
BtsI GCAGTG_nn'

Cuts at:    556    2378     556 
   Size:       1822    3313
 
DraI TTT'AAA

Cuts at:   2134    2134 
   Size:       5135
 
DraIII CAC_nnn'GTG

Cuts at:   1114    1114 
   Size:       5135
 
DrdI GACnn_nn'nnGTC

Cuts at:    849    2841    3699     849 
   Size:       1992     858    2285

  Fragments arranged by size: 

                2285    1992     858
 
EaeI y'GGCC_r

Cuts at:    516    1141    1411    1634    2066    2720    2894    3285    3312 
   Size:        625     270     223     432     654     174     391      27

Cuts at:   3312    4657     516 
   Size:       1345     994

  Fragments arranged by size: 

                1345     994     654     625     432     391     270     223
                 174      27
 
EagI C'GGCC_G

Cuts at:   2720    2720 
   Size:       5135
 
EarI CTCTTCn'nnn_

Cuts at:    341     596     611     782    1028    1157    1208    1242    1577 
   Size:        255      15     171     246     129      51      34     335

Cuts at:   1577    3158    3368     341 
   Size:       1581     210    2108

  Fragments arranged by size: 

                2108    1581     335     255     246     210     171     129
                  51      34      15
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   3343    3653    3799    4945    3343 
   Size:        310     146    1146    3533

  Fragments arranged by size: 

                3533    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   2147    2147 
   Size:       5135
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    225     307     365     377     395     443     635     728     848 
   Size:         82      58      12      18      48     192      93     120

Cuts at:    848    1144    1527    1670    2960    3392    4139    4407     225 
   Size:        296     383     143    1290     432     747     268     953

  Fragments arranged by size: 

                1290     953     747     432     383     296     268     192
                 143     120      93      82      58      48      18      12
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:   1250    1250 
   Size:       5135
 
EcoO109I rG'GnC_Cy

Cuts at:    219     226     370     881     995    1535    1845    1846    2274 
   Size:          7     144     511     114     540     310       1     428

Cuts at:   2274     219 
   Size:       3080

  Fragments arranged by size: 

                3080     540     511     428     310     144     114       7
                   1
 
EcoRI G'AATT_C

Cuts at:   2139    2139 
   Size:       5135
 
EcoRV GAT'ATC

Cuts at:   1558    1558 
   Size:       5135
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1794    3154    1794 
   Size:       1360    3775
 
FseI GG_CCGG'CC

Cuts at:   1828    1828 
   Size:       5135
 
FspI TGC'GCA

Cuts at:   2916    2916 
   Size:       5135
 
HaeII r_GCGC'y

Cuts at:   1719    2569    2817    3839    4818    1719 
   Size:        850     248    1022     979    2036

  Fragments arranged by size: 

                2036    1022     979     850     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    865     897    1180    1186    1212    1218    1315    1347    1622 
   Size:         32     283       6      26       6      97      32     275

Cuts at:   1622    1654    2977    3007    3009    3039     865 
   Size:         32    1323      30       2      30    2961

  Fragments arranged by size: 

                2961    1323     283     275      97      32      32      32
                  30      30      26       6       6       2
 
HincII GTy'rAC

Cuts at:    630    2174     630 
   Size:       1544    3591
 
HpaI GTT'AAC

Cuts at:    630     630 
   Size:       5135
 
KasI G'GCGC_C

Cuts at:   2565    2813    2565 
   Size:        248    4887
 
KpnI G_GTAC'C

Cuts at:   2155    2155 
   Size:       5135
 
MluI A'CGCG_T

Cuts at:   4568    4568 
   Size:       5135
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    896    1719    3806    3990     896 
   Size:        823    2087     184    2041

  Fragments arranged by size: 

                2087    2041     823     184
 
MscI TGG'CCA

Cuts at:    518    1143    1636    2068    2896     518 
   Size:        625     493     432     828    2757

  Fragments arranged by size: 

                2757     828     625     493     432
 
MslI CAynn'nnrTG

Cuts at:    766    3124    3251     766 
   Size:       2358     127    2650

  Fragments arranged by size: 

                2650    2358     127
 
MspA1I CmG'CkG

Cuts at:   1066    2098    2246    2560    2920    3933    4178    1066 
   Size:       1032     148     314     360    1013     245    2023

  Fragments arranged by size: 

                2023    1032    1013     360     314     245     148
 
NaeI GCC'GGC

Cuts at:   1826    3316    1826 
   Size:       1490    3645
 
NarI GG'CG_CC

Cuts at:   2566    2814    2566 
   Size:        248    4887
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       5135
 
NgoMIV G'CCGG_C

Cuts at:   1824    3314    1824 
   Size:       1490    3645
 
NspI r_CATG'y

Cuts at:    459    2188    2688    3595     459 
   Size:       1729     500     907    1999

  Fragments arranged by size: 

                1999    1729     907     500
 
PasI CC'CwG_GG

Cuts at:    580     778     964    1670    1850     580 
   Size:        198     186     706     180    3865

  Fragments arranged by size: 

                3865     706     198     186     180
 
PciI A'CATG_T

Cuts at:   3591    3591 
   Size:       5135
 
PflMI CCAn_nnn'nTGG

Cuts at:    764     884    1806    1952    2064     764 
   Size:        120     922     146     112    3835

  Fragments arranged by size: 

                3835     922     146     120     112
 
PfoI T'CCnGG_A

Cuts at:    744     744 
   Size:       5135
 
PmeI GTTT'AAAC

Cuts at:   2134    2134 
   Size:       5135
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4274    4306    4274 
   Size:         32    5103
 
PpuMI rG'GwC_Cy

Cuts at:    226     881     226 
   Size:        655    4480
 
PspOMI G'GGCC_C

Cuts at:   1535    1845    1535 
   Size:        310    4825
 
PstI C_TGCA'G

Cuts at:   2097    2182    2867    2097 
   Size:         85     685    4365

  Fragments arranged by size: 

                4365     685      85
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       5135
 
PvuII CAG'CTG

Cuts at:   1066    2560    2920    1066 
   Size:       1494     360    3281

  Fragments arranged by size: 

                3281    1494     360
 
RsrII CG'GwC_CG

Cuts at:   3330    3330 
   Size:       5135
 
SacI G_AGCT'C

Cuts at:   2149    2149 
   Size:       5135
 
SalI G'TCGA_C

Cuts at:   2172    2172 
   Size:       5135
 
SapI GCTCTTCn'nnn_

Cuts at:   3158    3368    3158 
   Size:        210    4925
 
SbfI CC_TGCA'GG

Cuts at:   2182    2182 
   Size:       5135
 
ScaI AGT'ACT

Cuts at:   4652    4652 
   Size:       5135
 
SfcI C'TryA_G

Cuts at:     33    1250    2093    2178    2863    3856    4047      33 
   Size:       1217     843      85     685     993     191    1121

  Fragments arranged by size: 

                1217    1121     993     843     685     191      85
 
SfoI GGC'GCC

Cuts at:   2567    2815    2567 
   Size:        248    4887
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5135
 
SmaI CCC'GGG

Cuts at:   1942    2157    1942 
   Size:        215    4920
 
SmlI C'TyrA_G

Cuts at:   1003    1711    2052    2290    3205    3697    3959    1003 
   Size:        708     341     238     915     492     262    2179

  Fragments arranged by size: 

                2179     915     708     492     341     262     238
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       5135
 
SphI G_CATG'C

Cuts at:   2188    2688    2188 
   Size:        500    4635
 
SrfI GCCC'GGGC

Cuts at:   1942    1942 
   Size:       5135
 
StuI AGG'CCT

Cuts at:   1129    1399    1129 
   Size:        270    4865
 
StyI C'CwwG_G

Cuts at:    107     328    1487    1681    2269    2623     107 
   Size:        221    1159     194     588     354    2619

  Fragments arranged by size: 

                2619    1159     588     354     221     194
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    756    1122    1392    1912    2014    2718    2851    3310    3519 
   Size:        366     270     520     102     704     133     459     209

Cuts at:   3519     756 
   Size:       2372

  Fragments arranged by size: 

                2372     704     520     459     366     270     209     133
                 102
 
TatI w'GTAC_w

Cuts at:   4650    4650 
   Size:       5135
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   4652    4684    4652 
   Size:         32    5103
 
Tth111I GACn'n_nGTC

Cuts at:   2932    2932 
   Size:       5135
 
XbaI T'CTAG_A

Cuts at:    231    2166     231 
   Size:       1935    3200
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1667    2630    1667 
   Size:        963    4172
 
XmaI C'CCGG_G

Cuts at:   1940    2155    1940 
   Size:        215    4920
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AhdI     AleI
    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI    AvrII
    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I     BmrI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI
    BseYI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstYI   Bsu36I     BtgI
    BtgZI     BtsI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    HaeII    Hin4I   HincII     HpaI     KasI     KpnI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NspI     PasI     PciI    PflMI     PfoI     PmeI     PpiI
    PpuMI   PspOMI     PstI     PvuI    PvuII    RsrII     SacI     SalI
     SapI     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI
     SpeI     SphI     SrfI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AlfI     AloI     AscI     BaeI
    BfrBI    BmgBI     BmtI    BsaXI     BsgI    BsiWI     BsmI    BsmBI
    BsrGI   BssHII   BstEII    BstXI  BstZ17I     ClaI    CspCI    FspAI
  HindIII     MfeI     NdeI     NheI     NotI     NruI     NsiI     PacI
     PmlI    PshAI     PsiI    PspXI     PsrI    SacII    SanDI    SexAI
     SfiI    SgrAI    SnaBI     SspI     SwaI     XhoI     XmnI     ZraI
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