Restriction map for pF1KB6426
 (Linear) (Six-base) MAPSORT of: KIBB6426.seq  Check: 8326  from: 1  to: 858



KIBB6426.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:47  ..

AhdI GACnn_n'nnGTC

Cuts at:      0     408     858 
   Size:        408     450
 
AleI CACnn'nnGTG

Cuts at:      0     245     448     858 
   Size:        245     203     410

  Fragments arranged by size: 

                 410     245     203
 
AlwNI CAG_nnn'CTG

Cuts at:      0     465     858 
   Size:        465     393
 
AvaI C'yCGr_G

Cuts at:      0     115     211     858 
   Size:        115      96     647

  Fragments arranged by size: 

                 647     115      96
 
BanI G'GyrC_C

Cuts at:      0      41     706     858 
   Size:         41     665     152

  Fragments arranged by size: 

                 665     152      41
 
BbeI G_GCGC'C

Cuts at:      0      45     710     858 
   Size:         45     665     148

  Fragments arranged by size: 

                 665     148      45
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     629     858 
   Size:        629     229
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     622     656     858 
   Size:        622      34     202

  Fragments arranged by size: 

                 622     202      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     389     858 
   Size:        389     469
 
BglII A'GATC_T

Cuts at:      0     371     858 
   Size:        371     487
 
BmgBI CAC'GTC

Cuts at:      0     504     858 
   Size:        504     354
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     830     858 
   Size:        830      28
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     739     858 
   Size:        739     119
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     166     721     858 
   Size:        166     555     137

  Fragments arranged by size: 

                 555     166     137
 
BsaHI Gr'CG_yC

Cuts at:      0      42     533     707     858 
   Size:         42     491     174     151

  Fragments arranged by size: 

                 491     174     151      42
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     542     572     858 
   Size:        542      30     286

  Fragments arranged by size: 

                 542     286      30
 
BseYI C'CCAG_C

Cuts at:      0     512     858 
   Size:        512     346
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     526     858 
   Size:        526     332
 
BsiEI CG_ry'CG

Cuts at:      0     528     858 
   Size:        528     330
 
BsiHKAI G_wGCw'C

Cuts at:      0     609     742     858 
   Size:        609     133     116

  Fragments arranged by size: 

                 609     133     116
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     285     540     858 
   Size:        285     255     318

  Fragments arranged by size: 

                 318     285     255
 
Bsp1286I G_dGCh'C

Cuts at:      0     609     742     858 
   Size:        609     133     116

  Fragments arranged by size: 

                 609     133     116
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     413     485     858 
   Size:        413      72     373

  Fragments arranged by size: 

                 413     373      72
 
BssSI C'ACGA_G

Cuts at:      0     241     858 
   Size:        241     617
 
BstYI r'GATC_y

Cuts at:      0     371     749     858 
   Size:        371     378     109

  Fragments arranged by size: 

                 378     371     109
 
BtgI C'CryG_G

Cuts at:      0     231     384     528     858 
   Size:        231     153     144     330

  Fragments arranged by size: 

                 330     231     153     144
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     333     858 
   Size:        333     525
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     666     701     858 
   Size:        666      35     157

  Fragments arranged by size: 

                 666     157      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     615     858 
   Size:        615     243
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     532     858 
   Size:        532     326
 
EaeI y'GGCC_r

Cuts at:      0      66     353     563     858 
   Size:         66     287     210     295

  Fragments arranged by size: 

                 295     287     210      66
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     444     831     858 
   Size:        444     387      27

  Fragments arranged by size: 

                 444     387      27
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     830     858 
   Size:        830      28
 
EcoO109I rG'GnC_Cy

Cuts at:      0     462     858 
   Size:        462     396
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     183     603     858 
   Size:        183     420     255

  Fragments arranged by size: 

                 420     255     183
 
HaeII r_GCGC'y

Cuts at:      0      45     710     858 
   Size:         45     665     148

  Fragments arranged by size: 

                 665     148      45
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     394     426     858 
   Size:        394      32     432

  Fragments arranged by size: 

                 432     394      32
 
KasI G'GCGC_C

Cuts at:      0      41     706     858 
   Size:         41     665     152

  Fragments arranged by size: 

                 665     152      41
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      29     858 
   Size:         29     829
 
MscI TGG'CCA

Cuts at:      0     565     858 
   Size:        565     293
 
MslI CAynn'nnrTG

Cuts at:      0     245     448     858 
   Size:        245     203     410

  Fragments arranged by size: 

                 410     245     203
 
MspA1I CmG'CkG

Cuts at:      0     654     858 
   Size:        654     204
 
NarI GG'CG_CC

Cuts at:      0      42     707     858 
   Size:         42     665     151

  Fragments arranged by size: 

                 665     151      42
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     775     807     858 
   Size:        775      32      51

  Fragments arranged by size: 

                 775      51      32
 
PstI C_TGCA'G

Cuts at:      0     410     858 
   Size:        410     448
 
PvuII CAG'CTG

Cuts at:      0     654     858 
   Size:        654     204
 
ScaI AGT'ACT

Cuts at:      0      73     858 
   Size:         73     785
 
SexAI A'CCwGG_T

Cuts at:      0     611     858 
   Size:        611     247
 
SfcI C'TryA_G

Cuts at:      0     406     858 
   Size:        406     452
 
SfoI GGC'GCC

Cuts at:      0      43     708     858 
   Size:         43     665     150

  Fragments arranged by size: 

                 665     150      43
 
SmlI C'TyrA_G

Cuts at:      0     754     858 
   Size:        754     104
 
StuI AGG'CCT

Cuts at:      0     392     858 
   Size:        392     466
 
StyI C'CwwG_G

Cuts at:      0     167     858 
   Size:        167     691
 
TatI w'GTAC_w

Cuts at:      0      71     858 
   Size:         71     787
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     542     574     858 
   Size:        542      32     284

  Fragments arranged by size: 

                 542     284      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     651     717     858 
   Size:        651      66     141

  Fragments arranged by size: 

                 651     141      66
 
XmnI GAAnn'nnTTC

Cuts at:      0     220     319     858 
   Size:        220      99     539

  Fragments arranged by size: 

                 539     220      99
 

 Enzymes that do cut:

     AhdI     AleI    AlwNI     AvaI     BanI     BbeI     BbsI     BcgI
     BglI    BglII    BmgBI     BpmI    BpuEI     BsaI    BsaHI    BsaXI
    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspMI    BssSI
    BstYI     BtgI    BtgZI    CspCI   DraIII     DrdI     EaeI     EciI
  Eco57MI EcoO109I     FalI    HaeII    Hin4I     KasI     MmeI     MscI
     MslI   MspA1I     NarI     PsrI     PstI    PvuII     ScaI    SexAI
     SfcI     SfoI     SmlI     StuI     StyI     TatI     TstI     XcmI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI    BanII    BbvCI    BciVI
     BclI    BfrBI     BlpI Bme1580I     BmrI     BmtI     BplI   Bpu10I
    BsaAI    BsaBI    BsaWI    BseRI    BsiWI     BsmI    BspEI    BspHI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtsI     ClaI     DraI     EagI     EarI
  EcoICRI    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI   HincII
  HindIII     HpaI     KpnI     MfeI     MluI     NaeI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     SfiI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     SwaI    TaqII  Tth111I     XbaI
     XhoI     XmaI     ZraI
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