Restriction map for pF1KB6439
##################################
# NO CUT ENZYMES (  132 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       AgeI        AhdI        AlwNI       Aor51HI     
ApaI        AscI        AseI        AsnI        Asp718I     
AspEI       AvaI        AviII       AvrII       BanI        
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BclI        BcnI        BfrI        BglI        BglII       
BlnI        BlpI        BpmI        Bpu1102I    BsaBI       
BsaHI       BsaWI       BseAI       BseRI       BsgI        
BsiEI       BsiWI       BsmBI       BsmI        BsoBI       
BspDI       BspEI       BspMI       BssHII      BssSI       
Bst1107I    BstBI       BstXI       BstZ17I     CelII       
ClaI        CpoI        DraI        DraII       DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoNI       EcoO109I    EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HaeII       
Hin1I       HincII      HindII      HindIII     HpaI        
KasI        KpnI        KspI        MamI        MfeI        
MluI        MroI        MunI        NarI        NciI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RsrII       SacI        SacII       SalI        SbfI        
ScaI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspI        SwaI        Van91I      XbaI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   71 enzymes) #
###################################

AccI          695  GTCTAC               GT^[AC][GT]AC
AccII          16  CGCG                 CG^CG
AflIII        751  ACATGT               A^C[GA][CT]GT
Alw44I        525  GTGCAC               G^TGCAC
AocI          418  CCTCAGG              CC^T[ACGT]AGG
ApaLI         525  GTGCAC               G^TGCAC
Asp700I       648  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         525  GTGCAC               G[AT]GC[AT]^C
AspI          689  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaII         688  GGACC                G^G[AT]CC
BamHI         871  GGATCC               G^GATCC
BanII         388  GAGCCC               G[GA]GC[CT]^C
BbsI          321  GAAGAC               GAAGAC(2/6)
BfaI          814  CTAG                 C^TAG
BpuAI         321  GAAGAC               GAAGAC(2/6)
BsaAI          25  TACGTG               [CT]AC^GT[GA]
BsaI          373  GAGACC               GGTCTC(1/5)
BsiHKAI       525  GTGCAC               G[AT]GC[AT]^C
BsiYI         638  CCATCTATGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          638  CCATCTATGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI         687  GGGAC                GGGAC(10/14)
Bsp1407I      425  TGTACA               T^GTACA
BspHI         531  TCATGA               T^CATGA
BspLU11I      751  ACATGT               A^CATGT
BsrBI         408  CCGCTC               CCGCTC(-3/-3)
BsrDI         393  CATTGC               GCAATG(2/0)
BsrFI         453  GCCGGC               [GA]^CCGG[CT]
BsrGI         425  TGTACA               T^GTACA
BstAPI        169  GCAGAGGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        801  GGTCACC              G^GT[ACGT]ACC
BstPI         801  GGTCACC              G^GT[ACGT]ACC
BstUI          16  CGCG                 CG^CG
BstYI         871  GGATCC               [GA]^GATC[CT]
Bsu36I        418  CCTCAGG              CC^T[ACGT]AGG
CfoI           15  GCGC                 GCG^C
Cfr10I        453  GCCGGC               [GA]^CCGG[CT]
DpnII         872  GATC                 ^GATC
DsaI          704  CCATGG               C^C[GA][CT]GG
Eco81I        418  CCTCAGG              CC^T[ACGT]AGG
EcoO65I       801  GGTCACC              G^GT[ACGT]ACC
FauI          407  CCCGC                CCCGC(4/6)
HapII         454  CCGG                 C^CGG
HhaI           15  GCGC                 GCG^C
HinP1I         15  GCGC                 G^CGC
HpaII         454  CCGG                 C^CGG
MaeI          814  CTAG                 C^TAG
MboI          872  GATC                 ^GATC
MflI          871  GGATCC               [GA]^GATC[CT]
MslI          465  CATCCAGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        516  CCGCTG               C[AC]G^C[GT]G
MspI          454  CCGG                 C^CGG
MvnI           16  CGCG                 CG^CG
NaeI          453  GCCGGC               GCC^GGC
NcoI          704  CCATGG               C^CATGG
NdeII         872  GATC                 ^GATC
NgoMIV        453  GCCGGC               G^CCGGC
PflFI         689  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PstI          179  CTGCAG               CTGCA^G
RcaI          531  TCATGA               T^CATGA
SapI          269  GCTCTTC              GCTCTTC(1/4)
Sau3AI        872  GATC                 ^GATC
SexAI         690  ACCAGGT              A^CC[AT]GGT
SfcI          179  CTGCAG               C^T[GA][CT]AG
SmlI          676  CTCAAG               C^T[CT][GA]AG
SspBI         425  TGTACA               T^GTACA
StuI          810  AGGCCT               AGG^CCT
TspRI         624  CACTG                CA[GC]TG[ACGT][ACGT]^
Tth111I       689  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI          615  CCACAATGGCACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         871  GGATCC               [GA]^GATC[CT]
XmnI          648  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AcsI          157  GAATTT               [GA]^AATT[CT]
              510  GAATTT              
AlwI          872  GATCC                GGATC(4/5)
              871  GGATC               
ApoI          157  GAATTT               [GA]^AATT[CT]
              510  GAATTT              
BsmAI          89  GAGAC                GTCTC(1/5)
              373  GAGAC               
EarI          270  CTCTTC               CTCTTC(1/4)
              331  GAAGAG              
EcoT14I       704  CCATGG               C^C[AT][AT]GG
              851  CCAAGG              
HgaI          324  GACGC                GACGC(5/10)
               17  GCGTC               
HinfI         108  GATTC                G^A[ACGT]TC
              205  GAATC               
HphI          601  GGTGA                GGTGA(8/7)
              803  TCACC               
Ksp632I       270  CTCTTC               CTCTTC(1/4)
              331  GAAGAG              
MaeII          26  ACGT                 A^CGT
              222  ACGT                
MaeIII        755  GTGAC                ^GT[ACGT]AC
              802  GTCAC               
MseI          260  TTAA                 T^TAA
              551  TTAA                
NlaIV         687  GGGACC               GG[ACGT]^[ACGT]CC
              871  GGATCC              
NspI          751  ACATGT               [GA]CATG^[CT]
              783  GCATGT              
StyI          704  CCATGG               C^C[AT][AT]GG
              851  CCAAGG              
TaqI          191  TCGA                 T^CGA
              656  TCGA                
TfiI          108  GATTC                G^A[AT]TC
              205  GAATC               
Tru9I         260  TTAA                 T^TAA
              551  TTAA                
Tsp45I        755  GTGAC                ^GT[GC]AC
              802  GTCAC               
TthHB8I       191  TCGA                 T^CGA
              656  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   34 enzymes) #
#########################################

AciI        AfaI        AluI        BalI        BbvI        
BmyI        BsaJI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       Cac8I       Cfr13I      DdeI        
EaeI        EcoRII      Fnu4HI      FokI        HaeIII      
ItaI        MboII       MluNI       MnlI        MscI        
MvaI        MwoI        NlaIII      RsaI        Sau96I      
ScrFI       SfaNI       TseI        Tsp509I     
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