Restriction map for pF1KB6702
 (Linear) (Six-base) MAPSORT of: KIBB6702.seq  Check: 6863  from: 1  to: 384



KIBB6702.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:47  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      51     355     384 
   Size:         51     304      29

  Fragments arranged by size: 

                 304      51      29
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     313     384 
   Size:        313      71
 
AlwNI CAG_nnn'CTG

Cuts at:      0      66     384 
   Size:         66     318
 
ApoI r'AATT_y

Cuts at:      0     212     384 
   Size:        212     172
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     255     289     384 
   Size:        255      34      95

  Fragments arranged by size: 

                 255      95      34
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      36     384 
   Size:         36     348
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      51     355     384 
   Size:         51     304      29

  Fragments arranged by size: 

                 304      51      29
 
BsrDI GCAATG_nn'

Cuts at:      0      79     261     384 
   Size:         79     182     123

  Fragments arranged by size: 

                 182     123      79
 
BsrGI T'GTAC_A

Cuts at:      0     348     384 
   Size:        348      36
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      66     384 
   Size:         66     318
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     313     384 
   Size:        313      71
 
EcoRI G'AATT_C

Cuts at:      0     212     384 
   Size:        212     172
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     147     384 
   Size:        147     237
 
MspA1I CmG'CkG

Cuts at:      0     185     384 
   Size:        185     199
 
NspI r_CATG'y

Cuts at:      0     192     384 
   Size:        192     192
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     237     384 
   Size:        237     147
 
TatI w'GTAC_w

Cuts at:      0     348     384 
   Size:        348      36
 
XbaI T'CTAG_A

Cuts at:      0     156     384 
   Size:        156     228
 

 Enzymes that do cut:

     AarI     AcuI    AlwNI     ApoI     BcgI     BsaI    BspMI    BsrDI
    BsrGI   BstAPI  Eco57MI    EcoRI     FalI   MspA1I     NspI    PflMI
     TatI     XbaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI
    BsrBI    BsrFI   BssHII    BssSI    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRV     FseI     FspI    FspAI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     StyI     SwaI    TaqII     TstI  Tth111I     XcmI     XhoI     XmaI
     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com