Restriction map for pF1KE0475
 (Linear) (Six-base) MAPSORT of: KIEE0475.seq  Check: 9876  from: 1  to: 756



KIEE0475.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:14  ..

AccI GT'mk_AC

Cuts at:      0     557     756 
   Size:        557     199
 
AhdI GACnn_n'nnGTC

Cuts at:      0     553     756 
   Size:        553     203
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     165     756 
   Size:        165     591
 
ApoI r'AATT_y

Cuts at:      0     276     713     756 
   Size:        276     437      43

  Fragments arranged by size: 

                 437     276      43
 
AvaI C'yCGr_G

Cuts at:      0      92     756 
   Size:         92     664
 
BanI G'GyrC_C

Cuts at:      0      20     756 
   Size:         20     736
 
BanII G_rGCy'C

Cuts at:      0      53     756 
   Size:         53     703
 
BbeI G_GCGC'C

Cuts at:      0      24     756 
   Size:         24     732
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     364     756 
   Size:        364     392
 
BbvCI CC'TCA_GC

Cuts at:      0      61     756 
   Size:         61     695
 
BglII A'GATC_T

Cuts at:      0     678     756 
   Size:        678      78
 
Bme1580I G_kGCm'C

Cuts at:      0     184     756 
   Size:        184     572
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     277     756 
   Size:        277     479
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     590     756 
   Size:        590     166
 
Bpu10I CC'TnA_GC

Cuts at:      0      61     756 
   Size:         61     695
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     124     290     756 
   Size:        124     166     466

  Fragments arranged by size: 

                 466     166     124
 
BsaBI GATnn'nnATC

Cuts at:      0     677     756 
   Size:        677      79
 
BsaHI Gr'CG_yC

Cuts at:      0      21     756 
   Size:         21     735
 
BsaWI w'CCGG_w

Cuts at:      0     493     756 
   Size:        493     263
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      88     661     756 
   Size:         88     573      95

  Fragments arranged by size: 

                 573      95      88
 
BseYI C'CCAG_C

Cuts at:      0     621     756 
   Size:        621     135
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     161     413     756 
   Size:        161     252     343

  Fragments arranged by size: 

                 343     252     161
 
BsiHKAI G_wGCw'C

Cuts at:      0     738     756 
   Size:        738      18
 
Bsp1286I G_dGCh'C

Cuts at:      0      53     184     738     756 
   Size:         53     131     554      18

  Fragments arranged by size: 

                 554     131      53      18
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     543     756 
   Size:        543     213
 
BsrGI T'GTAC_A

Cuts at:      0     306     756 
   Size:        306     450
 
BstYI r'GATC_y

Cuts at:      0     393     678     756 
   Size:        393     285      78

  Fragments arranged by size: 

                 393     285      78
 
BtgI C'CryG_G

Cuts at:      0     125     589     756 
   Size:        125     464     167

  Fragments arranged by size: 

                 464     167     125
 
BtsI GCAGTG_nn'

Cuts at:      0     144     335     430     756 
   Size:        144     191      95     326

  Fragments arranged by size: 

                 326     191     144      95
 
EarI CTCTTCn'nnn_

Cuts at:      0     589     756 
   Size:        589     167
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     590     756 
   Size:        590     166
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     189     756 
   Size:        189     567
 
EcoRI G'AATT_C

Cuts at:      0     276     756 
   Size:        276     480
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     201     412     756 
   Size:        201     211     344

  Fragments arranged by size: 

                 344     211     201
 
HaeII r_GCGC'y

Cuts at:      0      24     756 
   Size:         24     732
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     539     571     756 
   Size:        539      32     185

  Fragments arranged by size: 

                 539     185      32
 
HincII GTy'rAC

Cuts at:      0     558     756 
   Size:        558     198
 
KasI G'GCGC_C

Cuts at:      0      20     756 
   Size:         20     736
 
NarI GG'CG_CC

Cuts at:      0      21     756 
   Size:         21     735
 
NspI r_CATG'y

Cuts at:      0      60     446     756 
   Size:         60     386     310

  Fragments arranged by size: 

                 386     310      60
 
PacI TTA_AT'TAA

Cuts at:      0     206     756 
   Size:        206     550
 
PfoI T'CCnGG_A

Cuts at:      0     169     756 
   Size:        169     587
 
SalI G'TCGA_C

Cuts at:      0     556     756 
   Size:        556     200
 
ScaI AGT'ACT

Cuts at:      0      79     756 
   Size:         79     677
 
SfcI C'TryA_G

Cuts at:      0     746     756 
   Size:        746      10
 
SfoI GGC'GCC

Cuts at:      0      22     756 
   Size:         22     734
 
SmaI CCC'GGG

Cuts at:      0      94     756 
   Size:         94     662
 
SphI G_CATG'C

Cuts at:      0     446     756 
   Size:        446     310
 
SspI AAT'ATT

Cuts at:      0     285     756 
   Size:        285     471
 
StyI C'CwwG_G

Cuts at:      0     185     756 
   Size:        185     571
 
TatI w'GTAC_w

Cuts at:      0      77     306     756 
   Size:         77     229     450

  Fragments arranged by size: 

                 450     229      77
 
XmaI C'CCGG_G

Cuts at:      0      92     756 
   Size:         92     664
 

 Enzymes that do cut:

     AccI     AhdI     AlfI     ApoI     AvaI     BanI    BanII     BbeI
     BbsI    BbvCI    BglII Bme1580I     BmrI     BpmI   Bpu10I     BsaI
    BsaBI    BsaHI    BsaWI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I
    BspMI    BsrGI    BstYI     BtgI     BtsI     EarI  Eco57MI    EcoNI
    EcoRI     FalI    HaeII    Hin4I   HincII     KasI     NarI     NspI
     PacI     PfoI     SalI     ScaI     SfcI     SfoI     SmaI     SphI
     SspI     StyI     TatI     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BcgI    BciVI     BclI    BfrBI
     BglI     BlpI    BmgBI     BmtI     BplI    BpuEI    BsaAI    BsaXI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI
    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EciI  EcoICRI EcoO109I    EcoRV     FseI     FspI    FspAI
  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII    SanDI     SapI     SbfI    SexAI     SfiI
     SgfI    SgrAI     SmlI    SnaBI     SpeI     SrfI     StuI     SwaI
    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmnI     ZraI
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