Restriction map for pF1KB8177
##################################
# NO CUT ENZYMES (  130 enzymes) #
##################################

AatII       AccII       AccIII      AciI        AclI        
AcyI        AflII       AgeI        AhdI        AocI        
Aor51HI     ApaI        AscI        Asp700I     AspEI       
AvaI        AviII       AvrII       BalI        BamHI       
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BfrI        BglI        BlnI        BlpI        Bpu1102I    
BsaAI       BsaHI       BsaWI       BseAI       BsiEI       
BsiWI       BsmBI       BsmFI       BsoBI       BspDI       
BspEI       BspHI       BsrBI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstXI       BstZ17I     Bsu36I      CelII       
ClaI        CpoI        DraI        DraIII      DrdI        
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoO65I     EcoT22I     FauI        
FseI        FspI        HaeII       Hin1I       HindIII     
HpaI        KasI        KspI        MfeI        MluI        
MluNI       MroI        MscI        MunI        MvnI        
NaeI        NarI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PinAI       PmaCI       PmeI        PmlI        
PshAI       Psp1406I    PstI        PvuI        RcaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    StuI        SwaI        TaqI        TthHB8I     
XbaI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

Acc65I        486  GGTACC               G^GTACC
AccI         1376  GTCTAC               GT^[AC][GT]AC
AflIII        557  ACATGT               A^C[GA][CT]GT
AseI          544  ATTAAT               AT^TAAT
AsnI          544  ATTAAT               AT^TAAT
Asp718I       486  GGTACC               G^GTACC
AspI          348  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BanI          486  GGTACC               G^G[CT][GA]CC
BglII         875  AGATCT               A^GATCT
BsaBI         925  GATCAGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI          251  GAATGC               GAATGC(1/-1)
BspLU11I      557  ACATGT               A^CATGT
BspMI         525  ACCTGC               ACCTGC(4/8)
CfoI          758  GCGC                 GCG^C
DraII        1255  AGGACCT              [GA]G^G[ACGT]CC[CT]
EarI         1593  GAAGAG               CTCTTC(1/4)
EcoNI        1011  CCTCCATAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I     1255  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoRI         501  GAATTC               G^AATTC
EcoRV         260  GATATC               GAT^ATC
HgaI         1374  GCGTC                GACGC(5/10)
HhaI          758  GCGC                 GCG^C
HinP1I        758  GCGC                 G^CGC
HincII       1516  GTCAAC               GT[CT]^[GA]AC
HindII       1516  GTCAAC               GT[CT]^[GA]AC
KpnI          486  GGTACC               GGTAC^C
Ksp632I      1593  GAAGAG               CTCTTC(1/4)
MamI          925  GATCAGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NspI          557  ACATGT               [GA]CATG^[CT]
PflFI         348  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         685  CCAGATGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI        1255  AGGACCT              [GA]G^G[AT]CC[CT]
PshBI         544  ATTAAT               AT^TAAT
SfcI          662  CTACAG               C^T[GA][CT]AG
SmlI            5  CTCAAG               C^T[CT][GA]AG
SspI         1092  AATATT               AAT^ATT
Tth111I       348  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        685  CCAGATGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

Alw44I        199  GTGCAC               G^TGCAC
             1584  GTGCAC              
AlwNI         506  CAGACCCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1475  CAGCTCCTG           
ApaLI         199  GTGCAC               G^TGCAC
             1584  GTGCAC              
AvaII         777  GGACC                G^G[AT]CC
             1256  GGACC               
BanII         634  GAGCCC               G[GA]GC[CT]^C
              676  GAGCCC              
BbsI         1141  GAAGAC               GAAGAC(2/6)
              864  GTCTTC              
BclI          924  TGATCA               T^GATCA
             1469  TGATCA              
BcnI          240  CCGGG                CC^[GC]GG
              266  CCCGG               
BpuAI        1141  GAAGAC               GAAGAC(2/6)
              864  GTCTTC              
BsgI          234  GTGCAG               GTGCAG(16/14)
             1433  GTGCAG              
Bsp1407I      293  TGTACA               T^GTACA
              468  TGTACA              
BsrDI        1410  CATTGC               GCAATG(2/0)
             1150  GCAATG              
BsrFI          91  ACCGGC               [GA]^CCGG[CT]
              380  ACCGGC              
BsrGI         293  TGTACA               T^GTACA
              468  TGTACA              
Cfr10I         91  ACCGGC               [GA]^CCGG[CT]
              380  ACCGGC              
DsaI          395  CCATGG               C^C[GA][CT]GG
              413  CCATGG              
FbaI          924  TGATCA               T^GATCA
             1469  TGATCA              
MseI           17  TTAA                 T^TAA
              545  TTAA                
MspA1I        356  CAGCTG               C[AC]G^C[GT]G
             1289  CAGCTG              
NciI          240  CCGGG                CC^[GC]GG
              266  CCCGG               
NcoI          395  CCATGG               C^CATGG
              413  CCATGG              
PvuII         356  CAGCTG               CAG^CTG
             1289  CAGCTG              
SexAI         182  ACCTGGT              A^CC[AT]GGT
              563  ACCTGGT             
SspBI         293  TGTACA               T^GTACA
              468  TGTACA              
TfiI          722  GATTC                G^A[AT]TC
             1155  GATTC               
Tru9I          17  TTAA                 T^TAA
              545  TTAA                
XcmI         1037  CCACAGCCACTCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1556  CCATCTTCCTGCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   63 enzymes) #
#########################################

AcsI        AfaI        AluI        AlwI        ApoI        
AspHI       BbvI        BfaI        BmyI        BpmI        
BsaI        BsaJI       BseRI       BsiHKAI     BsiYI       
BslI        BsmAI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       BstYI       Cac8I       Cfr13I      
DdeI        DpnII       EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HapII       HinfI       HpaII       
HphI        ItaI        MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MslI        
MspI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       PleI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       StyI        TseI        Tsp45I      
Tsp509I     TspRI       XhoII       
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