Restriction map for pF1KB6406
##################################
# NO CUT ENZYMES (  154 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwI        AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AspHI       AspI        AviII       AvrII       
BalI        BamHI       BbrPI       BbsI        BbvCI       
BcgI        BciVI       BclI        BfrI        BglI        
BlnI        BlpI        Bpu1102I    BpuAI       BsaBI       
BsaI        BseAI       BseRI       BsiEI       BsiHKAI     
BsiWI       BsmBI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrFI       BsrGI       BssHII      Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstXI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoO109I    EcoO65I     
EcoT22I     FauI        FbaI        FseI        FspI        
HaeIII      HgaI        HincII      HindII      HindIII     
HpaI        KpnI        KspI        MamI        MfeI        
MluI        MluNI       MroI        MscI        MspA1I      
MunI        MvnI        NaeI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfuI        SgrAI       SmaI        SpeI        SphI        
Sse8387I    SspBI       StuI        SwaI        Tth111I     
Van91I      XbaI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AciI           19  CCGC                 CCGC(-3/-1)
AcsI          446  GAATTC               [GA]^AATT[CT]
AcyI           15  GGCGCC               G[GA]^CG[CT]C
AlwNI         125  CAGCATCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApoI          446  GAATTC               [GA]^AATT[CT]
Asp700I       256  GAAAAGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI          302  CTCGAG               C^[CT]CG[GA]G
BanI           15  GGCGCC               G^G[CT][GA]CC
BanII         848  GGGCTC               G[GA]GC[CT]^C
BbeI           15  GGCGCC               GGCGC^C
BcnI          981  CCGGG                CC^[GC]GG
BglII         369  AGATCT               A^GATCT
BmyI          848  GGGCTC               G[AGT]GC[ACT]^C
BsaAI         245  TACGTA               [CT]AC^GT[GA]
BsaHI          15  GGCGCC               G[GA]^CG[CT]C
BsaWI         995  ACCGGA               [AT]^CCGG[AT]
BsgI          551  GTGCAG               GTGCAG(16/14)
BsoBI         302  CTCGAG               C^[CT]CG[GA]G
Bsp1286I      848  GGGCTC               G[AGT]GC[ACT]^C
BsrDI         519  CATTGC               GCAATG(2/0)
BssSI         452  CTCGTG               CACGAG(-5/-1)
BstNI         187  CCTGG                CC^[AT]GG
BstYI         369  AGATCT               [GA]^GATC[CT]
Cac8I         716  GCCAGC               GC[ACGT]^[ACGT]GC
CfoI           16  GCGC                 GCG^C
EcoRI         446  GAATTC               G^AATTC
EcoRII        187  CCTGG                ^CC[AT]GG
EcoRV         766  GATATC               GAT^ATC
HaeII          15  GGCGCC               [GA]GCGC^[CT]
HhaI           16  GCGC                 GCG^C
Hin1I          15  GGCGCC               G[GA]^CG[CT]C
HinP1I         16  GCGC                 G^CGC
KasI           15  GGCGCC               G^GCGCC
MflI          369  AGATCT               [GA]^GATC[CT]
MslI           45  CACCGACATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI          187  CCTGG                CC^[AT]GG
MwoI          408  GCGAATTGGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NarI           15  GGCGCC               GG^CGCC
NciI          981  CCGGG                CC^[GC]GG
NspI           50  ACATGC               [GA]CATG^[CT]
PaeR7I        302  CTCGAG               C^TCGAG
SfaNI         127  GCATC                GCATC(5/9)
SfcI          524  CTACAG               C^T[GA][CT]AG
SfoI           15  GGCGCC               GGC^GCC
SmlI          302  CTCGAG               C^T[CT][GA]AG
SnaBI         245  TACGTA               TAC^GTA
SspI          422  AATATT               AAT^ATT
TaqI          303  TCGA                 T^CGA
TthHB8I       303  TCGA                 T^CGA
XhoI          302  CTCGAG               C^TCGAG
XhoII         369  AGATCT               [GA]^GATC[CT]
XmnI          256  GAAAAGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AvaII         319  GGTCC                G^G[AT]CC
              993  GGACC               
BbvI          124  GCAGC                GCAGC(8/12)
              328  GCTGC               
BfaI          364  CTAG                 C^TAG
              859  CTAG                
BpmI          188  CTGGAG               CTGGAG(16/14)
              911  CTGGAG              
BsaJI         899  CCTTGG               C^C[ACGT][ACGT]GG
              989  CCAAGG              
BsmAI          25  GAGAC                GTCTC(1/5)
              694  GAGAC               
BsmFI         431  GGGAC                GGGAC(10/14)
              896  GTCCC               
BssKI         187  CCTGG                ^CC[ACGT]GG
              981  CCGGG               
Cfr13I        319  GGTCC                G^G[ACGT]CC
              993  GGACC               
DdeI          176  CTAAG                C^T[ACGT]AG
              373  CTCAG               
DpnII         370  GATC                 ^GATC
              787  GATC                
EarI          457  GAAGAG               CTCTTC(1/4)
              545  GAAGAG              
EcoT14I       899  CCTTGG               C^C[AT][AT]GG
              989  CCAAGG              
HapII         981  CCGG                 C^CGG
              996  CCGG                
HpaII         981  CCGG                 C^CGG
              996  CCGG                
Ksp632I       457  GAAGAG               CTCTTC(1/4)
              545  GAAGAG              
MaeI          364  CTAG                 C^TAG
              859  CTAG                
MaeII          83  ACGT                 A^CGT
              246  ACGT                
MboI          370  GATC                 ^GATC
              787  GATC                
MseI          356  TTAA                 T^TAA
              497  TTAA                
MspI          981  CCGG                 C^CGG
              996  CCGG                
NdeII         370  GATC                 ^GATC
              787  GATC                
NlaIV          15  GGCGCC               GG[ACGT]^[ACGT]CC
              984  GGAACC              
Sau3AI        370  GATC                 ^GATC
              787  GATC                
Sau96I        319  GGTCC                G^G[ACGT]CC
              993  GGACC               
ScrFI         187  CCTGG                CC^[ACGT]GG
              981  CCGGG               
StyI          899  CCTTGG               C^C[AT][AT]GG
              989  CCAAGG              
Tru9I         356  TTAA                 T^TAA
              497  TTAA                
TseI          124  GCAGC                G^C[AT]GC
              328  GCTGC               
Tsp45I        566  GTGAC                ^GT[GC]AC
              907  GTCAC               
XcmI          560  CCAGTGGTGACATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              890  CCATCCGTCCCTTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   21 enzymes) #
#########################################

AfaI        AluI        BsiYI       BslI        BsrI        
BstF5I      EcoNI       Fnu4HI      FokI        HinfI       
HphI        ItaI        MaeIII      MboII       MnlI        
NlaIII      PleI        RsaI        TfiI        Tsp509I     
TspRI       
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