Restriction map for pF1KB6157
##################################
# NO CUT ENZYMES (  113 enzymes) #
##################################

AatII       Acc65I      AccII       AclI        AflIII      
AgeI        Aor51HI     ApaI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspI        AviII       
AvrII       BamHI       BbrPI       BcgI        BciVI       
BclI        BlnI        BpmI        BsaBI       BsiEI       
BsiWI       BsmBI       BsmI        Bsp1407I    BspDI       
BspHI       BspLU11I    BsrBI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstUI       
BstXI       BstZ17I     ClaI        CpoI        DraI        
DraII       DraIII      DrdI        DsaI        EagI        
EclXI       Eco47III    Eco52I      EcoNI       EcoO109I    
EcoRI       EcoRV       EcoT14I     FbaI        FseI        
FspI        HgaI        HpaI        KpnI        KspI        
MamI        MfeI        MluI        MunI        MvnI        
NcoI        NdeI        NotI        NruI        NspI        
NspV        PacI        PflFI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
StuI        StyI        SwaI        Tth111I     XbaI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   57 enzymes) #
###################################

AccIII       1465  TCCGGA               T^CCGGA
AcsI          848  AAATTC               [GA]^AATT[CT]
AcyI         1679  GGCGCC               G[GA]^CG[CT]C
AflII         871  CTTAAG               C^TTAAG
AhdI         1561  GACTATGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       1285  GTGCAC               G^TGCAC
AlwNI          16  CAGCAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI         1725  CCTTAGG              CC^T[ACGT]AGG
ApaLI        1285  GTGCAC               G^TGCAC
ApoI          848  AAATTC               [GA]^AATT[CT]
AspEI        1561  GACTATGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI         1679  GGCGCC               GGCGC^C
BbvCI        1629  GCTGAGG              CCTCAGC(-5/-2)
BcnI          825  CCGGG                CC^[GC]GG
BfrI          871  CTTAAG               C^TTAAG
BglI          764  GCCAGATTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI        1065  CACGTA               [CT]AC^GT[GA]
BsaHI        1679  GGCGCC               G[GA]^CG[CT]C
BsaI          821  GAGACC               GGTCTC(1/5)
BsaWI        1465  TCCGGA               [AT]^CCGG[AT]
BseAI        1465  TCCGGA               T^CCGGA
BsmFI        1267  GTCCC                GGGAC(10/14)
BspEI        1465  TCCGGA               T^CCGGA
BsrDI        1770  CATTGC               GCAATG(2/0)
BstEII        510  GGTTACC              G^GT[ACGT]ACC
BstPI         510  GGTTACC              G^GT[ACGT]ACC
Bsu36I       1725  CCTTAGG              CC^T[ACGT]AGG
CfoI         1680  GCGC                 GCG^C
Eam1105I     1561  GACTATGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       1725  CCTTAGG              CC^T[ACGT]AGG
EcoO65I       510  GGTTACC              G^GT[ACGT]ACC
EcoT22I      1767  ATGCAT               ATGCA^T
FauI         1536  CCCGC                CCCGC(4/6)
HaeII        1679  GGCGCC               [GA]GCGC^[CT]
HhaI         1680  GCGC                 GCG^C
Hin1I        1679  GGCGCC               G[GA]^CG[CT]C
HinP1I       1680  GCGC                 G^CGC
HincII        411  GTCAAC               GT[CT]^[GA]AC
HindII        411  GTCAAC               GT[CT]^[GA]AC
HindIII       713  AAGCTT               A^AGCTT
KasI         1679  GGCGCC               G^GCGCC
MaeII        1066  ACGT                 A^CGT
MroI         1465  TCCGGA               T^CCGGA
MseI          872  TTAA                 T^TAA
MslI         1770  CATTGCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI         1734  GCCGGC               GCC^GGC
NarI         1679  GGCGCC               GG^CGCC
NciI          825  CCGGG                CC^[GC]GG
NgoMIV       1734  GCCGGC               G^CCGGC
NheI          729  GCTAGC               G^CTAGC
NsiI         1767  ATGCAT               ATGCA^T
PflMI          15  CCAGCAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SfoI         1679  GGCGCC               GGC^GCC
SspI          878  AATATT               AAT^ATT
Tru9I         872  TTAA                 T^TAA
Van91I         15  CCAGCAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         1688  CCAAAGGGCAGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AccI         1175  GTCTAC               GT^[AC][GT]AC
             1214  GTCTAC              
AlwI          180  GATCC                GGATC(4/5)
             1790  GGATC               
AspHI         842  GTGCTC               G[AT]GC[AT]^C
             1285  GTGCAC              
AvaII        1023  GGTCC                G^G[AT]CC
             1039  GGACC               
BalI          255  TGGCCA               TGG^CCA
             1553  TGGCCA              
BanI         1679  GGCGCC               G^G[CT][GA]CC
             1705  GGCACC              
BanII        1372  GAGCCC               G[GA]GC[CT]^C
             1761  GAGCCC              
BbsI         1195  GAAGAC               GAAGAC(2/6)
             1546  GAAGAC              
BglII          38  AGATCT               A^GATCT
             1422  AGATCT              
BlpI          524  GCTTAGC              GC^T[ACGT]AGC
             1150  GCTAAGC             
Bpu1102I      524  GCTTAGC              GC^T[ACGT]AGC
             1150  GCTAAGC             
BpuAI        1195  GAAGAC               GAAGAC(2/6)
             1546  GAAGAC              
BsgI          144  CTGCAC               GTGCAG(16/14)
             1777  GTGCAG              
BsiHKAI       842  GTGCTC               G[AT]GC[AT]^C
             1285  GTGCAC              
BsrFI        1109  ACCGGC               [GA]^CCGG[CT]
             1734  GCCGGC              
CelII         524  GCTTAGC              GC^T[ACGT]AGC
             1150  GCTAAGC             
Cfr10I       1109  ACCGGC               [GA]^CCGG[CT]
             1734  GCCGGC              
EarI          313  GAAGAG               CTCTTC(1/4)
              994  GAAGAG              
HphI          439  TCACC                GGTGA(8/7)
             1598  TCACC               
Ksp632I       313  GAAGAG               CTCTTC(1/4)
              994  GAAGAG              
MluNI         255  TGGCCA               TGG^CCA
             1553  TGGCCA              
MscI          255  TGGCCA               TGG^CCA
             1553  TGGCCA              
PaeR7I        814  CTCGAG               C^TCGAG
             1337  CTCGAG              
PleI         1718  GACTC                GAGTC(4/5)
             1567  GAGTC               
PstI           34  CTGCAG               CTGCA^G
             1377  CTGCAG              
PvuII          19  CAGCTG               CAG^CTG
             1588  CAGCTG              
SfcI           34  CTGCAG               C^T[GA][CT]AG
             1377  CTGCAG              
Tsp45I        751  GTCAC                ^GT[GC]AC
             1597  GTCAC               
XhoI          814  CTCGAG               C^TCGAG
             1337  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (   59 enzymes) #
#########################################

AciI        AfaI        AluI        AvaI        BbvI        
BfaI        BmyI        BsaJI       BseRI       BsiYI       
BslI        BsmAI       BsoBI       Bsp1286I    BspMI       
BsrI        BssKI       BstF5I      BstNI       BstYI       
Cac8I       Cfr13I      DdeI        DpnII       EaeI        
EcoRII      Fnu4HI      FokI        HaeIII      HapII       
HinfI       HpaII       ItaI        MaeI        MaeIII      
MboI        MboII       MflI        MnlI        MspA1I      
MspI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SmlI        TaqI        TfiI        TseI        
Tsp509I     TspRI       TthHB8I     XhoII       
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