Restriction map for pF1KB8899
##################################
# NO CUT ENZYMES (  150 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwI        AocI        Aor51HI     ApaLI       
ApoI        AscI        AseI        AsnI        Asp718I     
AspEI       AspHI       AspI        AviII       AvrII       
BalI        BamHI       BbrPI       BbsI        BciVI       
BclI        BfaI        BfrI        BglI        BlnI        
BlpI        Bpu1102I    BpuAI       BsaAI       BsaI        
BseAI       BseRI       BsiHKAI     BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstXI       BstZ17I     Bsu36I      CelII       Cfr10I      
ClaI        CpoI        DraI        DraIII      DrdI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HincII      
HindII      HindIII     HpaI        KpnI        Ksp632I     
KspI        MaeI        MaeIII      MboII       MfeI        
MluI        MluNI       MroI        MscI        MseI        
MslI        MunI        NaeI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PstI        PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfcI        SfiI        SfuI        
SgrAI       SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
Tru9I       Tsp45I      Tth111I     XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   32 enzymes) #
###################################

AlwNI         496  CAGACACTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          291  GGGCCC               GGGCC^C
Asp700I       513  GAAGGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbvCI         102  GCTGAGG              CCTCAGC(-5/-2)
BcgI          554  GCATCGAGATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII         599  AGATCT               A^GATCT
BsaBI         594  GATCAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI         386  ACCGGA               [AT]^CCGG[AT]
BsgI          307  CTGCAC               GTGCAG(16/14)
BsiEI         384  CGACCG               CG[GA][CT]^CG
BsiWI         122  CGTACG               C^GTACG
BsmAI         137  GTCTC                GTCTC(1/5)
BsmFI         299  GGGAC                GGGAC(10/14)
BspMI         305  ACCTGC               ACCTGC(4/8)
BsrI          501  ACTGG                ACTGG(1/-1)
BstYI         599  AGATCT               [GA]^GATC[CT]
DsaI          459  CCATGG               C^C[GA][CT]GG
EaeI           48  CGGCCA               [CT]^GGCC[GA]
EcoNI          78  CCTCTACCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
MaeII         151  ACGT                 A^CGT
MamI          594  GATCAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          599  AGATCT               [GA]^GATC[CT]
NcoI          459  CCATGG               C^CATGG
PflMI         495  CCAGACACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         248  AGGACCT              [GA]G^G[AT]CC[CT]
PvuII          70  CAGCTG               CAG^CTG
TfiI           18  GAATC                G^A[AT]TC
Tsp509I       652  AATT                 ^AATT
Van91I        495  CCAGACACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          182  CCAACTCGGTCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         599  AGATCT               [GA]^GATC[CT]
XmnI          513  GAAGGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AvaII         189  GGTCC                G^G[AT]CC
              249  GGACC               
BanII         291  GGGCCC               G[GA]GC[CT]^C
              399  GAGCCC              
BmyI          291  GGGCCC               G[AGT]GC[ACT]^C
              399  GAGCCC              
BpmI          502  CTGGAG               CTGGAG(16/14)
              508  CTGGAG              
Bsp1286I      291  GGGCCC               G[AGT]GC[ACT]^C
              399  GAGCCC              
BssSI         396  CACGAG               CACGAG(-5/-1)
              228  CTCGTG              
BstF5I        416  GGATG                GGATG(2/0)
              425  GGATG               
EcoT14I       412  CCTTGG               C^C[AT][AT]GG
              459  CCATGG              
FokI          416  GGATG                GGATG(9/13)
              425  GGATG               
HphI          160  TCACC                GGTGA(8/7)
              581  TCACC               
SfaNI         417  GATGC                GCATC(5/9)
              554  GCATC               
SmaI           31  CCCGGG               CCC^GGG
              295  CCCGGG              
StyI          412  CCTTGG               C^C[AT][AT]GG
              459  CCATGG              
TaqI          133  TCGA                 T^CGA
              557  TCGA                
TthHB8I       133  TCGA                 T^CGA
              557  TCGA                
XmaCI          31  CCCGGG               C^CCGGG
              295  CCCGGG              
XmaI           31  CCCGGG               C^CCGGG
              295  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   59 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AvaI        BanI        BbeI        BbvI        BcnI        
BsaHI       BsaJI       BsiYI       BslI        BsoBI       
BssKI       BstNI       BstUI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       EcoO109I    
EcoRII      FauI        Fnu4HI      HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       ItaI        KasI        MboI        
MnlI        MspA1I      MspI        MvaI        MvnI        
MwoI        NarI        NciI        NdeII       NlaIII      
NlaIV       PleI        RsaI        Sau3AI      Sau96I      
ScrFI       SfoI        TseI        TspRI       
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