Restriction map for pF1KB9547
##################################
# NO CUT ENZYMES (  190 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AfaI        AflII       
AflIII      AgeI        AlwNI       AocI        Aor51HI     
ApaI        ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspI        AvaI        AvrII       
BalI        BamHI       BanI        BbeI        BbrPI       
BbvCI       BcgI        BciVI       BclI        BcnI        
BfaI        BfrI        BglI        BglII       BlnI        
BlpI        BpmI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BsaWI       BseAI       BseRI       BsiEI       
BsiWI       BsmBI       BsmI        BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BsrI        
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       BstZ17I     
Bsu36I      CelII       Cfr10I      ClaI        CpoI        
DraI        DraIII      DrdI        DsaI        EaeI        
EagI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FauI        FbaI        FseI        HaeIII      
HapII       Hin1I       HincII      HindII      HindIII     
HpaI        HpaII       HphI        KasI        KpnI        
KspI        MaeI        MaeII       MamI        MfeI        
MluI        MluNI       MroI        MscI        MseI        
MspA1I      MspI        MunI        MvnI        NaeI        
NarI        NciI        NcoI        NgoMIV      NheI        
NlaIII      NlaIV       NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsaI        RsrII       SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        StyI        
SwaI        TaqI        TfiI        Tru9I       Tsp509I     
TspRI       Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AciI           33  CCGC                 CCGC(-3/-1)
AhdI          188  GACCTGGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AluI          277  AGCT                 AG^CT
Alw44I        222  GTGCAC               G^TGCAC
AlwI          154  GGATC                GGATC(4/5)
ApaLI         222  GTGCAC               G^TGCAC
AspEI         188  GACCTGGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII         187  GGACC                G^G[AT]CC
AviII         128  TGCGCA               TGC^GCA
BanII         276  GAGCTC               G[GA]GC[CT]^C
BbsI           59  GAAGAC               GAAGAC(2/6)
BbvI          251  GCTGC                GCAGC(8/12)
BpuAI          59  GAAGAC               GAAGAC(2/6)
BsaI          195  GGTCTC               GGTCTC(1/5)
BsgI          117  CTGCAC               GTGCAG(16/14)
BsmAI         196  GTCTC                GTCTC(1/5)
BsmFI         113  GTCCC                GGGAC(10/14)
BstYI         154  GGATCT               [GA]^GATC[CT]
Cfr13I        187  GGACC                G^G[ACGT]CC
DdeI          281  CTAAG                C^T[ACGT]AG
DraII         186  AGGACCT              [GA]G^G[ACGT]CC[CT]
Eam1105I      188  GACCTGGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I      186  AGGACCT              [GA]G^G[ACGT]CC[CT]
FspI          128  TGCGCA               TGC^GCA
HaeII         167  GGCGCT               [GA]GCGC^[CT]
HgaI          111  GCGTC                GACGC(5/10)
HinfI         100  GACTC                G^A[ACGT]TC
MflI          154  GGATCT               [GA]^GATC[CT]
MslI          120  CACTCTGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MwoI           43  GCTGGTGTTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI          132  CATATG               CA^TATG
PleI          100  GACTC                GAGTC(4/5)
PpuMI         186  AGGACCT              [GA]G^G[AT]CC[CT]
SacI          276  GAGCTC               GAGCT^C
Sau96I        187  GGACC                G^G[ACGT]CC
SfaNI         202  GATGC                GCATC(5/9)
TseI          251  GCTGC                G^C[AT]GC
Tsp45I         98  GTGAC                ^GT[GC]AC
XhoII         154  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AspHI         222  GTGCAC               G[AT]GC[AT]^C
              276  GAGCTC              
BmyI          222  GTGCAC               G[AGT]GC[ACT]^C
              276  GAGCTC              
BsaJI         162  CCAGGG               C^C[ACGT][ACGT]GG
              190  CCTGGG              
BsiHKAI       222  GTGCAC               G[AT]GC[AT]^C
              276  GAGCTC              
BsiYI          33  CCGCAAGCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              231  CCAGGAGAAGG         
BslI           33  CCGCAAGCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              231  CCAGGAGAAGG         
Bsp1286I      222  GTGCAC               G[AGT]GC[ACT]^C
              276  GAGCTC              
BstF5I         13  CATCC                GGATG(2/0)
              228  CATCC               
Cac8I          35  GCAAGC               GC[ACGT]^[ACGT]GC
               39  GCAGGC              
CfoI          129  GCGC                 GCG^C
              168  GCGC                
EarI          292  CTCTTC               CTCTTC(1/4)
              324  GAAGAG              
Fnu4HI         32  GCCGC                GC^[ACGT]GC
              251  GCTGC               
FokI           13  CATCC                GGATG(9/13)
              228  CATCC               
HhaI          129  GCGC                 GCG^C
              168  GCGC                
HinP1I        129  GCGC                 G^CGC
              168  GCGC                
ItaI           32  GCCGC                GC^[ACGT]GC
              251  GCTGC               
Ksp632I       292  CTCTTC               CTCTTC(1/4)
              324  GAAGAG              
MaeIII         98  GTGAC                ^GT[ACGT]AC
              146  GTAAC               


#########################################
# 3 OR MORE CUT ENZYMES (   11 enzymes) #
#########################################

BssKI       BstNI       DpnII       EcoRII      MboI        
MboII       MnlI        MvaI        NdeII       Sau3AI      
ScrFI       
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