(Linear) (Six-base) MAPSORT of: KIBB6738.seq  Check: 9086  from: 1  to: 420



KIBB6738.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

AclI AA'CG_TT

Cuts at:      0     115     420 
   Size:        115     305
 
AgeI A'CCGG_T

Cuts at:      0     164     277     420 
   Size:        164     113     143

  Fragments arranged by size: 

                 164     143     113
 
ApaI G_GGCC'C

Cuts at:      0     288     420 
   Size:        288     132
 
ApoI r'AATT_y

Cuts at:      0     247     420 
   Size:        247     173
 
AvaI C'yCGr_G

Cuts at:      0     106     420 
   Size:        106     314
 
BanII G_rGCy'C

Cuts at:      0     288     420 
   Size:        288     132
 
BbvCI CC'TCA_GC

Cuts at:      0     137     420 
   Size:        137     283
 
BclI T'GATC_A

Cuts at:      0     368     420 
   Size:        368      52
 
Bme1580I G_kGCm'C

Cuts at:      0     288     420 
   Size:        288     132
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     408     420 
   Size:        408      12
 
Bpu10I CC'TnA_GC

Cuts at:      0     137     420 
   Size:        137     283
 
BsaAI yAC'GTr

Cuts at:      0     180     420 
   Size:        180     240
 
BsaWI w'CCGG_w

Cuts at:      0     164     277     420 
   Size:        164     113     143

  Fragments arranged by size: 

                 164     143     113
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      70     100     420 
   Size:         70      30     320

  Fragments arranged by size: 

                 320      70      30
 
BsiHKAI G_wGCw'C

Cuts at:      0     277     420 
   Size:        277     143
 
Bsp1286I G_dGCh'C

Cuts at:      0     277     288     420 
   Size:        277      11     132

  Fragments arranged by size: 

                 277     132      11
 
BsrFI r'CCGG_y

Cuts at:      0     164     277     420 
   Size:        164     113     143

  Fragments arranged by size: 

                 164     143     113
 
BstYI r'GATC_y

Cuts at:      0     258     420 
   Size:        258     162
 
BtgI C'CryG_G

Cuts at:      0     357     420 
   Size:        357      63
 
BtsI GCAGTG_nn'

Cuts at:      0     229     420 
   Size:        229     191
 
EaeI y'GGCC_r

Cuts at:      0       2      59     420 
   Size:          2      57     361

  Fragments arranged by size: 

                 361      57       2
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      52     420 
   Size:         52     368
 
EcoO109I rG'GnC_Cy

Cuts at:      0      44     284     285     420 
   Size:         44     240       1     135

  Fragments arranged by size: 

                 240     135      44       1
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      70     102     420 
   Size:         70      32     318

  Fragments arranged by size: 

                 318      70      32
 
HincII GTy'rAC

Cuts at:      0     123     420 
   Size:        123     297
 
MspA1I CmG'CkG

Cuts at:      0     136     420 
   Size:        136     284
 
PfoI T'CCnGG_A

Cuts at:      0     221     420 
   Size:        221     199
 
PpuMI rG'GwC_Cy

Cuts at:      0      44     420 
   Size:         44     376
 
PshAI GACnn'nnGTC

Cuts at:      0     328     420 
   Size:        328      92
 
PspOMI G'GGCC_C

Cuts at:      0     284     420 
   Size:        284     136
 
PstI C_TGCA'G

Cuts at:      0     239     420 
   Size:        239     181
 
PvuII CAG'CTG

Cuts at:      0     136     420 
   Size:        136     284
 
SfcI C'TryA_G

Cuts at:      0     235     420 
   Size:        235     185
 
SgrAI Cr'CCGG_yG

Cuts at:      0     277     420 
   Size:        277     143
 
SmaI CCC'GGG

Cuts at:      0     108     420 
   Size:        108     312
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     133     420 
   Size:        133     287
 
XmaI C'CCGG_G

Cuts at:      0     106     420 
   Size:        106     314
 
XmnI GAAnn'nnTTC

Cuts at:      0     115     420 
   Size:        115     305
 

 Enzymes that do cut:

     AclI     AgeI     ApaI     ApoI     AvaI    BanII    BbvCI     BclI
 Bme1580I     BmrI   Bpu10I    BsaAI    BsaWI    BsaXI  BsiHKAI Bsp1286I
    BsrFI    BstYI     BtgI     BtsI     EaeI     EciI EcoO109I    Hin4I
   HincII   MspA1I     PfoI    PpuMI    PshAI   PspOMI     PstI    PvuII
     SfcI    SgrAI     SmaI    TaqII     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AcuI     AfeI    AflII   AflIII
     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI     BcgI
    BciVI    BfrBI     BglI    BglII     BlpI    BmgBI     BmtI     BplI
     BpmI    BpuEI     BsaI    BsaBI    BsaHI    BseRI    BseYI     BsgI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EarI  EcoICRI  Eco57MI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PmeI     PmlI     PpiI     PsiI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     ZraI