Restriction map for pF1KE0513
##################################
# NO CUT ENZYMES (  131 enzymes) #
##################################

Acc65I      AccII       AflII       AgeI        Alw44I      
AocI        ApaI        ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspI        AviII       
AvrII       BamHI       BanI        BbeI        BbrPI       
BbsI        BbvCI       BcgI        BciVI       BclI        
BfaI        BfrI        BglI        BglII       BlnI        
BpuAI       BsaAI       BsaBI       BsaI        BsiEI       
BsiWI       BsmAI       BsmBI       BsmI        Bsp1407I    
BspDI       BspHI       BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstYI       
BstZ17I     Bsu36I      Cfr10I      ClaI        CpoI        
DraI        DrdI        EagI        EclXI       Eco52I      
Eco81I      EcoO65I     EcoT22I     FauI        FbaI        
FseI        FspI        HindIII     HpaI        KasI        
KpnI        KspI        MaeI        MamI        MfeI        
MflI        MluI        MunI        MvnI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       PvuI        RcaI        RsrII       
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        Tth111I     
XbaI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   56 enzymes) #
###################################

AatII         586  GACGTC               GACGT^C
AccI          411  GTCTAC               GT^[AC][GT]AC
AccIII        615  TCCGGA               T^CCGGA
AclI          475  AACGTT               AA^CGTT
AfaI           18  GTAC                 GT^AC
AflIII        212  ACATGT               A^C[GA][CT]GT
AhdI          403  GACCTGTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI          158  GGATC                GGATC(4/5)
Aor51HI       443  AGCGCT               AGC^GCT
AspEI         403  GACCTGTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI          178  CCCGAG               C^[CT]CG[GA]G
BalI          437  TGGCCA               TGG^CCA
BcnI          110  CCCGG                CC^[GC]GG
BlpI          861  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      861  GCTCAGC              GC^T[ACGT]AGC
BseAI         615  TCCGGA               T^CCGGA
BsmFI         965  GGGAC                GGGAC(10/14)
BsoBI         178  CCCGAG               C^[CT]CG[GA]G
BspEI         615  TCCGGA               T^CCGGA
BspLU11I      212  ACATGT               A^CATGT
BsrI          708  CCAGT                ACTGG(1/-1)
BstXI          38  CCAAGGGGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII         861  GCTCAGC              GC^T[ACGT]AGC
DpnII         159  GATC                 ^GATC
DraIII        184  CACATTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          978  CCATGG               C^C[GA][CT]GG
EaeI          437  TGGCCA               [CT]^GGCC[GA]
Eam1105I      403  GACCTGTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          170  CTCTTC               CTCTTC(1/4)
Eco47III      443  AGCGCT               AGC^GCT
EcoNI         201  CCTGGAGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         778  GAATTC               G^AATTC
EcoRV         886  GATATC               GAT^ATC
HgaI          972  GACGC                GACGC(5/10)
HincII        658  GTCAAC               GT[CT]^[GA]AC
HindII        658  GTCAAC               GT[CT]^[GA]AC
HphI          824  TCACC                GGTGA(8/7)
Ksp632I       170  CTCTTC               CTCTTC(1/4)
MaeIII        823  GTCAC                ^GT[ACGT]AC
MboI          159  GATC                 ^GATC
MluNI         437  TGGCCA               TGG^CCA
MroI          615  TCCGGA               T^CCGGA
MscI          437  TGGCCA               TGG^CCA
MslI          318  CACAGTGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI          110  CCCGG                CC^[GC]GG
NcoI          978  CCATGG               C^CATGG
NdeII         159  GATC                 ^GATC
NspI          212  ACATGT               [GA]CATG^[CT]
PleI          345  GAGTC                GAGTC(4/5)
Psp1406I      475  AACGTT               AA^CGTT
RsaI           18  GTAC                 GT^AC
SacI           67  GAGCTC               GAGCT^C
Sau3AI        159  GATC                 ^GATC
SmlI          262  CTCAAG               C^T[CT][GA]AG
TfiI          696  GAATC                G^A[AT]TC
Tsp45I        823  GTCAC                ^GT[GC]AC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AcsI          124  AAATTT               [GA]^AATT[CT]
              778  GAATTC              
AcyI          586  GACGTC               G[GA]^CG[CT]C
              972  GACGCC              
ApoI          124  AAATTT               [GA]^AATT[CT]
              778  GAATTC              
BsaHI         586  GACGTC               G[GA]^CG[CT]C
              972  GACGCC              
BsaWI         341  ACCGGA               [AT]^CCGG[AT]
              615  TCCGGA              
BspMI          23  ACCTGC               ACCTGC(4/8)
               95  ACCTGC              
BstF5I        228  CATCC                GGATG(2/0)
              900  GGATG               
DdeI          862  CTCAG                C^T[ACGT]AG
              916  CTGAG               
EcoT14I        38  CCAAGG               C^C[AT][AT]GG
              978  CCATGG              
FokI          228  CATCC                GGATG(9/13)
              900  GGATG               
Hin1I         586  GACGTC               G[GA]^CG[CT]C
              972  GACGCC              
HinfI         345  GAGTC                G^A[ACGT]TC
              696  GAATC               
MseI          605  TTAA                 T^TAA
              772  TTAA                
NlaIII        213  CATG                 CATG^
              979  CATG                
PflMI         246  CCAGAGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              663  CCAGAAGCTGG         
PpuMI         116  AGGACCC              [GA]G^G[AT]CC[CT]
              401  AGGACCT             
PvuII         352  CAGCTG               CAG^CTG
              864  CAGCTG              
SfaNI         901  GATGC                GCATC(5/9)
              389  GCATC               
StyI           38  CCAAGG               C^C[AT][AT]GG
              978  CCATGG              
TaqI          218  TCGA                 T^CGA
              500  TCGA                
Tru9I         605  TTAA                 T^TAA
              772  TTAA                
TthHB8I       218  TCGA                 T^CGA
              500  TCGA                
Van91I        246  CCAGAGGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              663  CCAGAAGCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   48 enzymes) #
#########################################

AciI        AluI        AlwNI       AspHI       AvaII       
BanII       BbvI        BmyI        BpmI        BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
Bsp1286I    BssKI       BstNI       Cac8I       CfoI        
Cfr13I      DraII       EcoO109I    EcoRII      Fnu4HI      
HaeII       HaeIII      HapII       HhaI        HinP1I      
HpaII       ItaI        MaeII       MboII       MnlI        
MspA1I      MspI        MvaI        MwoI        NlaIV       
PstI        Sau96I      ScrFI       SfcI        TseI        
Tsp509I     TspRI       XcmI        
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