Restriction map for pF1KB6555
##################################
# NO CUT ENZYMES (  135 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        Asp718I     
AspEI       AviII       AvrII       BanI        BbeI        
BbrPI       BcgI        BciVI       BclI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BsiEI       BsiWI       BsmBI       BsmI        
BspDI       BspEI       BspHI       BspLU11I    BsrBI       
BsrDI       BsrFI       BssHII      BssSI       Bst1107I    
BstBI       BstZ17I     Bsu36I      Cac8I       CelII       
Cfr10I      ClaI        CpoI        DraI        DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HaeII       HgaI        
Hin1I       HpaI        KasI        KpnI        MamI        
MfeI        MluI        MroI        MunI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       Psp1406I    PvuI        
PvuII       RcaI        RsrII       SalI        SapI        
SbfI        ScaI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspI        StuI        SwaI        
Van91I      XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

Asp700I       390  GAAGCTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         994  GAGCTC               G[AT]GC[AT]^C
AspI         1042  GACATAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          208  CCCGAG               C^[CT]CG[GA]G
BamHI         252  GGATCC               G^GATCC
BbsI         1039  GAAGAC               GAAGAC(2/6)
BfaI          355  CTAG                 C^TAG
BpuAI        1039  GAAGAC               GAAGAC(2/6)
BsgI          928  CTGCAC               GTGCAG(16/14)
BsiHKAI       994  GAGCTC               G[AT]GC[AT]^C
BsoBI         208  CCCGAG               C^[CT]CG[GA]G
Bsp1407I     1082  TGTACA               T^GTACA
BsrGI        1082  TGTACA               T^GTACA
BstAPI        462  GCAAAAACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        435  GGTCACC              G^GT[ACGT]ACC
BstF5I        636  CATCC                GGATG(2/0)
BstPI         435  GGTCACC              G^GT[ACGT]ACC
BstYI         252  GGATCC               [GA]^GATC[CT]
CfoI          605  GCGC                 GCG^C
DraII         103  GGGACCC              [GA]G^G[ACGT]CC[CT]
DraIII        263  CACGCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI         1030  CCGCGG               C^C[GA][CT]GG
EarI         1090  CTCTTC               CTCTTC(1/4)
EcoO109I      103  GGGACCC              [GA]G^G[ACGT]CC[CT]
EcoO65I       435  GGTCACC              G^GT[ACGT]ACC
EcoT14I       128  CCAAGG               C^C[AT][AT]GG
FokI          636  CATCC                GGATG(9/13)
HhaI          605  GCGC                 GCG^C
HinP1I        605  GCGC                 G^CGC
HincII       1048  GTCAAC               GT[CT]^[GA]AC
HindII       1048  GTCAAC               GT[CT]^[GA]AC
Ksp632I      1090  CTCTTC               CTCTTC(1/4)
KspI         1030  CCGCGG               CCGC^GG
MaeI          355  CTAG                 C^TAG
MflI          252  GGATCC               [GA]^GATC[CT]
MspA1I       1030  CCGCGG               C[AC]G^C[GT]G
PflFI        1042  GACATAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PleI          617  GACTC                GAGTC(4/5)
PpuMI         103  GGGACCC              [GA]G^G[AT]CC[CT]
SacI          994  GAGCTC               GAGCT^C
SacII        1030  CCGCGG               CCGC^GG
SexAI         884  ACCTGGT              A^CC[AT]GGT
SspBI        1082  TGTACA               T^GTACA
StyI          128  CCAAGG               C^C[AT][AT]GG
TspRI         267  CAGTG                CA[GC]TG[ACGT][ACGT]^
Tth111I      1042  GACATAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoII         252  GGATCC               [GA]^GATC[CT]
XmnI          390  GAAGCTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AccII         604  CGCG                 CG^CG
             1031  CGCG                
AlwI          253  GATCC                GGATC(4/5)
              252  GGATC               
AseI          300  ATTAAT               AT^TAAT
              313  ATTAAT              
AsnI          300  ATTAAT               AT^TAAT
              313  ATTAAT              
BalI            2  TGGCCA               TGG^CCA
              563  TGGCCA              
BanII         901  GGGCTC               G[GA]GC[CT]^C
              994  GAGCTC              
BbvCI         180  CCTCAGC              CCTCAGC(-5/-2)
              430  GCTGAGG             
BbvI          144  GCTGC                GCAGC(8/12)
              891  GCTGC               
BseRI         151  GAGGAG               GAGGAG(10/8)
              226  GAGGAG              
BspMI         173  ACCTGC               ACCTGC(4/8)
              575  ACCTGC              
BstUI         604  CGCG                 CG^CG
             1031  CGCG                
BstXI         551  CCAAGAGCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              635  CCATCCAAGTGG        
DpnII         253  GATC                 ^GATC
              489  GATC                
FauI           12  CCCGC                CCCGC(4/6)
               65  CCCGC               
HindIII       277  AAGCTT               A^AGCTT
              391  AAGCTT              
MaeII         738  ACGT                 A^CGT
             1109  ACGT                
MboI          253  GATC                 ^GATC
              489  GATC                
MluNI           2  TGGCCA               TGG^CCA
              563  TGGCCA              
MscI            2  TGGCCA               TGG^CCA
              563  TGGCCA              
MseI          301  TTAA                 T^TAA
              314  TTAA                
MvnI          604  CGCG                 CG^CG
             1031  CGCG                
NdeII         253  GATC                 ^GATC
              489  GATC                
PshBI         300  ATTAAT               AT^TAAT
              313  ATTAAT              
Sau3AI        253  GATC                 ^GATC
              489  GATC                
Tru9I         301  TTAA                 T^TAA
              314  TTAA                
TseI          144  GCTGC                G^C[AT]GC
              891  GCTGC               
XcmI          551  CCAAGAGCCTGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              864  CCAGTTTGCAGATGG     


#########################################
# 3 OR MORE CUT ENZYMES (   48 enzymes) #
#########################################

AciI        AfaI        AluI        AvaII       BcnI        
BmyI        BpmI        BsaJI       BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BsrI        BssKI       
BstNI       Cfr13I      DdeI        EaeI        EcoRII      
Fnu4HI      HaeIII      HapII       HinfI       HpaII       
HphI        ItaI        MaeIII      MboII       MnlI        
MslI        MspI        MvaI        MwoI        NciI        
NlaIII      NlaIV       PstI        RsaI        Sau96I      
ScrFI       SfaNI       SfcI        TaqI        TfiI        
Tsp45I      Tsp509I     TthHB8I     
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