Restriction map for pF1KE0534
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# NO CUT ENZYMES (  117 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcsI        AfaI        AflII       AgeI        AhdI        
Alw44I      Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp718I     AspEI       
AspI        AviII       AvrII       BamHI       BbrPI       
BbsI        BbvCI       BciVI       BclI        BfrI        
BlnI        BpuAI       BsaAI       BsaBI       BsaWI       
BseAI       BsiWI       BsmBI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrDI       
BsrGI       BssHII      Bst1107I    BstBI       BstUI       
BstZ17I     ClaI        CpoI        DraI        DraIII      
Eam1105I    Eco47III    EcoNI       EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HindIII     
HpaI        KpnI        KspI        MamI        MfeI        
MluI        MroI        MunI        MvnI        NaeI        
NcoI        NdeI        NgoMIV      NotI        NruI        
NsiI        NspI        NspV        PacI        PflFI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsaI        
RsrII       SacII       SbfI        ScaI        SexAI       
SfiI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        Tth111I     XbaI        XcmI        
XmaCI       XmaI        


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# ONE CUT ENZYMES (   61 enzymes) #
###################################

AccI          418  GTCGAC               GT^[AC][GT]AC
AflIII        323  ACGTGT               A^C[GA][CT]GT
AlwI          143  GGATC                GGATC(4/5)
AocI          310  CCTCAGG              CC^T[ACGT]AGG
Asp700I       321  GAACGTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI          555  CTCGAG               C^[CT]CG[GA]G
BalI          740  TGGCCA               TGG^CCA
BbeI          115  GGCGCC               GGCGC^C
BcgI          557  CGAGGCCCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI           13  CTAG                 C^TAG
BglII         515  AGATCT               A^GATCT
BlpI         1217  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1217  GCTCAGC              GC^T[ACGT]AGC
BsaI          652  GAGACC               GGTCTC(1/5)
BsgI         1226  CTGCAC               GTGCAG(16/14)
BsiEI        1010  CGGCCG               CG[GA][CT]^CG
BsmAI         652  GAGAC                GTCTC(1/5)
BsoBI         555  CTCGAG               C^[CT]CG[GA]G
BsrBI         196  GAGCGG               CCGCTC(-3/-3)
BsrFI         275  ACCGGC               [GA]^CCGG[CT]
BssSI         736  CTCGTG               CACGAG(-5/-1)
BstAPI        123  GCAGAGGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI         641  CCAATGCCTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         515  AGATCT               [GA]^GATC[CT]
Bsu36I        310  CCTCAGG              CC^T[ACGT]AGG
CelII        1217  GCTCAGC              GC^T[ACGT]AGC
Cfr10I        275  ACCGGC               [GA]^CCGG[CT]
DrdI          964  GACATCCTCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         1010  CGGCCG               C^GGCCG
EarI          873  GAAGAG               CTCTTC(1/4)
EclXI        1010  CGGCCG               C^GGCCG
Eco52I       1010  CGGCCG               C^GGCCG
Eco81I        310  CCTCAGG              CC^T[ACGT]AGG
HgaI          354  GACGC                GACGC(5/10)
HincII        418  GTCGAC               GT[CT]^[GA]AC
HindII        418  GTCGAC               GT[CT]^[GA]AC
KasI          115  GGCGCC               G^GCGCC
Ksp632I       873  GAAGAG               CTCTTC(1/4)
MaeI           13  CTAG                 C^TAG
MaeII         323  ACGT                 A^CGT
MflI          515  AGATCT               [GA]^GATC[CT]
MluNI         740  TGGCCA               TGG^CCA
MscI          740  TGGCCA               TGG^CCA
MseI          674  TTAA                 T^TAA
MslI         1265  CACCAGGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        862  CAGCTG               C[AC]G^C[GT]G
NarI          115  GGCGCC               GG^CGCC
NheI           12  GCTAGC               G^CTAGC
NlaIII         30  CATG                 CATG^
PaeR7I        555  CTCGAG               C^TCGAG
PvuII         862  CAGCTG               CAG^CTG
SacI         1215  GAGCTC               GAGCT^C
SalI          418  GTCGAC               G^TCGAC
SapI          873  GAAGAGC              GCTCTTC(1/4)
SfoI          115  GGCGCC               GGC^GCC
TfiI          953  GAATC                G^A[AT]TC
Tru9I         674  TTAA                 T^TAA
Tsp509I       698  AATT                 ^AATT
XhoI          555  CTCGAG               C^TCGAG
XhoII         515  AGATCT               [GA]^GATC[CT]
XmnI          321  GAACGTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AcyI          115  GGCGCC               G[GA]^CG[CT]C
              354  GACGCC              
AspHI         385  GAGCAC               G[AT]GC[AT]^C
             1215  GAGCTC              
BglI          552  GCCCTCGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1189  GCCCGACTGGC         
BsaHI         115  GGCGCC               G[GA]^CG[CT]C
              354  GACGCC              
BseRI         172  CTCCTC               GAGGAG(10/8)
             1123  GAGGAG              
BsiHKAI       385  GAGCAC               G[AT]GC[AT]^C
             1215  GAGCTC              
BspMI        1067  ACCTGC               ACCTGC(4/8)
             1224  ACCTGC              
BstEII        336  GGTGACC              G^GT[ACGT]ACC
             1050  GGTCACC             
BstPI         336  GGTGACC              G^GT[ACGT]ACC
             1050  GGTCACC             
CfoI          116  GCGC                 GCG^C
              169  GCGC                
EcoO65I       336  GGTGACC              G^GT[ACGT]ACC
             1050  GGTCACC             
HaeII         115  GGCGCC               [GA]GCGC^[CT]
              168  GGCGCT              
HhaI          116  GCGC                 GCG^C
              169  GCGC                
Hin1I         115  GGCGCC               G[GA]^CG[CT]C
              354  GACGCC              
HinP1I        116  GCGC                 G^CGC
              169  GCGC                
MboII         873  GAAGA                GAAGA(8/7)
              329  TCTTC               
PleI          832  GACTC                GAGTC(4/5)
             1251  GAGTC               
SmlI          555  CTCGAG               C^T[CT][GA]AG
              940  CTCAAG              


#########################################
# 3 OR MORE CUT ENZYMES (   62 enzymes) #
#########################################

AciI        AluI        AlwNI       AvaII       BanI        
BanII       BbvI        BcnI        BmyI        BpmI        
BsaJI       BsiYI       BslI        BsmFI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       Cac8I       
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HinfI       
HpaII       HphI        ItaI        MaeIII      MboI        
MnlI        MspI        MvaI        MwoI        NciI        
NdeII       NlaIV       PflMI       PpuMI       PstI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
StyI        TaqI        TseI        Tsp45I      TspRI       
TthHB8I     Van91I      
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