Restriction map for pF1KE0534
 (Linear) (Six-base) MAPSORT of: KIEE0534.seq  Check: 5168  from: 1  to: 1323



KIEE0534.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:15  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1233    1323 
   Size:       1233      90
 
AccI GT'mk_AC

Cuts at:      0     419    1323 
   Size:        419     904
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     600     870    1323 
   Size:        600     270     453

  Fragments arranged by size: 

                 600     453     270
 
AflIII A'CryG_T

Cuts at:      0     323    1323 
   Size:        323    1000
 
AlwNI CAG_nnn'CTG

Cuts at:      0      22     104     129     509    1225    1323 
   Size:         22      82      25     380     716      98

  Fragments arranged by size: 

                 716     380      98      82      25      22
 
AvaI C'yCGr_G

Cuts at:      0     555    1323 
   Size:        555     768
 
BanI G'GyrC_C

Cuts at:      0     115     527    1024    1323 
   Size:        115     412     497     299

  Fragments arranged by size: 

                 497     412     299     115
 
BanII G_rGCy'C

Cuts at:      0      37    1001    1219    1323 
   Size:         37     964     218     104

  Fragments arranged by size: 

                 964     218     104      37
 
BbeI G_GCGC'C

Cuts at:      0     119    1323 
   Size:        119    1204
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     546     580    1323 
   Size:        546      34     743

  Fragments arranged by size: 

                 743     546      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     558    1195    1323 
   Size:        558     637     128

  Fragments arranged by size: 

                 637     558     128
 
BglII A'GATC_T

Cuts at:      0     515    1323 
   Size:        515     808
 
BlpI GC'TnA_GC

Cuts at:      0    1218    1323 
   Size:       1218     105
 
Bme1580I G_kGCm'C

Cuts at:      0       7     532     554    1027    1323 
   Size:          7     525      22     473     296

  Fragments arranged by size: 

                 525     473     296      22       7
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     207     816    1030    1323 
   Size:        207     609     214     293

  Fragments arranged by size: 

                 609     293     214     207
 
BmtI G_CTAG'C

Cuts at:      0      16    1323 
   Size:         16    1307
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     540     770    1128    1323 
   Size:        540     230     358     195

  Fragments arranged by size: 

                 540     358     230     195
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     925    1323 
   Size:        925     398
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     646    1323 
   Size:        646     677
 
BsaHI Gr'CG_yC

Cuts at:      0     116     355    1323 
   Size:        116     239     968

  Fragments arranged by size: 

                 968     239     116
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     308     338    1323 
   Size:        308      30     985

  Fragments arranged by size: 

                 985     308      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     163    1138    1323 
   Size:        163     975     185

  Fragments arranged by size: 

                 975     185     163
 
BseYI C'CCAG_C

Cuts at:      0      56     413     777    1041    1246    1323 
   Size:         56     357     364     264     205      77

  Fragments arranged by size: 

                 364     357     264     205      77      56
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1211    1323 
   Size:       1211     112
 
BsiEI CG_ry'CG

Cuts at:      0    1013    1323 
   Size:       1013     310
 
BsiHKAI G_wGCw'C

Cuts at:      0     389    1219    1323 
   Size:        389     830     104

  Fragments arranged by size: 

                 830     389     104
 
Bsp1286I G_dGCh'C

Cuts at:      0       7      37     389     532     554    1001    1027    1219 
   Size:          7      30     352     143      22     447      26     192

Cuts at:   1219    1323 
   Size:        104

  Fragments arranged by size: 

                 447     352     192     143     104      30      26      22
                   7
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1076    1233    1323 
   Size:       1076     157      90

  Fragments arranged by size: 

                1076     157      90
 
BsrBI CCG'CTC

Cuts at:      0     198    1323 
   Size:        198    1125
 
BsrFI r'CCGG_y

Cuts at:      0     275    1323 
   Size:        275    1048
 
BssSI C'ACGA_G

Cuts at:      0     736    1323 
   Size:        736     587
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     129    1323 
   Size:        129    1194
 
BstEII G'GTnAC_C

Cuts at:      0     336    1050    1323 
   Size:        336     714     273

  Fragments arranged by size: 

                 714     336     273
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     648    1323 
   Size:        648     675
 
BstYI r'GATC_y

Cuts at:      0     515    1323 
   Size:        515     808
 
Bsu36I CC'TnA_GG

Cuts at:      0     311    1323 
   Size:        311    1012
 
BtgI C'CryG_G

Cuts at:      0     459     533    1007    1232    1323 
   Size:        459      74     474     225      91

  Fragments arranged by size: 

                 474     459     225      91      74
 
BtsI GCAGTG_nn'

Cuts at:      0     678    1238    1323 
   Size:        678     560      85

  Fragments arranged by size: 

                 678     560      85
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     970    1323 
   Size:        970     353
 
EaeI y'GGCC_r

Cuts at:      0     290     740    1010    1196    1235    1323 
   Size:        290     450     270     186      39      88

  Fragments arranged by size: 

                 450     290     270     186      88      39
 
EagI C'GGCC_G

Cuts at:      0    1010    1323 
   Size:       1010     313
 
EarI CTCTTCn'nnn_

Cuts at:      0     868    1323 
   Size:        868     455
 
EcoICRI GAG'CTC

Cuts at:      0    1217    1323 
   Size:       1217     106
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     540     600     770     870    1128    1323 
   Size:        540      60     170     100     258     195

  Fragments arranged by size: 

                 540     258     195     170     100      60
 
EcoO109I rG'GnC_Cy

Cuts at:      0     201     497     506     560     660     732     755     765 
   Size:        201     296       9      54     100      72      23      10

Cuts at:    765    1134    1323 
   Size:        369     189

  Fragments arranged by size: 

                 369     296     201     189     100      72      54      23
                  10       9
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     645    1323 
   Size:        645     678
 
HaeII r_GCGC'y

Cuts at:      0     119     172    1323 
   Size:        119      53    1151

  Fragments arranged by size: 

                1151     119      53
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     376     408     882     914    1323 
   Size:        376      32     474      32     409

  Fragments arranged by size: 

                 474     409     376      32      32
 
HincII GTy'rAC

Cuts at:      0     420    1323 
   Size:        420     903
 
KasI G'GCGC_C

Cuts at:      0     115    1323 
   Size:        115    1208
 
MscI TGG'CCA

Cuts at:      0     742    1323 
   Size:        742     581
 
MslI CAynn'nnrTG

Cuts at:      0    1269    1323 
   Size:       1269      54
 
MspA1I CmG'CkG

Cuts at:      0     864    1323 
   Size:        864     459
 
NarI GG'CG_CC

Cuts at:      0     116    1323 
   Size:        116    1207
 
NheI G'CTAG_C

Cuts at:      0      12    1323 
   Size:         12    1311
 
PasI CC'CwG_GG

Cuts at:      0      40    1323 
   Size:         40    1283
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     104     483     902    1323 
   Size:        104     379     419     421

  Fragments arranged by size: 

                 421     419     379     104
 
PfoI T'CCnGG_A

Cuts at:      0    1312    1323 
   Size:       1312      11
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     310     342    1323 
   Size:        310      32     981

  Fragments arranged by size: 

                 981     310      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     201     497     506     660     732     755     765    1323 
   Size:        201     296       9     154      72      23      10     558

  Fragments arranged by size: 

                 558     296     201     154      72      23      10       9
            
 
PsiI TTA'TAA

Cuts at:      0      74    1323 
   Size:         74    1249
 
PspXI vC'TCGA_Gb

Cuts at:      0     555    1323 
   Size:        555     768
 
PstI C_TGCA'G

Cuts at:      0     125     137     869    1323 
   Size:        125      12     732     454

  Fragments arranged by size: 

                 732     454     125      12
 
PvuII CAG'CTG

Cuts at:      0     864    1323 
   Size:        864     459
 
SacI G_AGCT'C

Cuts at:      0    1219    1323 
   Size:       1219     104
 
SalI G'TCGA_C

Cuts at:      0     418    1323 
   Size:        418     905
 
SanDI GG'GwC_CC

Cuts at:      0     497     660    1323 
   Size:        497     163     663

  Fragments arranged by size: 

                 663     497     163
 
SapI GCTCTTCn'nnn_

Cuts at:      0     868    1323 
   Size:        868     455
 
SfcI C'TryA_G

Cuts at:      0     121     133     788     865    1323 
   Size:        121      12     655      77     458

  Fragments arranged by size: 

                 655     458     121      77      12
 
SfoI GGC'GCC

Cuts at:      0     117    1323 
   Size:        117    1206
 
SmlI C'TyrA_G

Cuts at:      0     555     940    1323 
   Size:        555     385     383

  Fragments arranged by size: 

                 555     385     383
 
StyI C'CwwG_G

Cuts at:      0     647     743    1130    1323 
   Size:        647      96     387     193

  Fragments arranged by size: 

                 647     387     193      96
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     769    1245    1309    1323 
   Size:        769     476      64      14

  Fragments arranged by size: 

                 769     476      64      14
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     308     340    1323 
   Size:        308      32     983

  Fragments arranged by size: 

                 983     308      32
 
XhoI C'TCGA_G

Cuts at:      0     555    1323 
   Size:        555     768
 
XmnI GAAnn'nnTTC

Cuts at:      0     325    1323 
   Size:        325     998
 

 Enzymes that do cut:

     AarI     AccI     AcuI   AflIII    AlwNI     AvaI     BanI    BanII
     BbeI     BcgI     BglI    BglII     BlpI Bme1580I     BmrI     BmtI
     BpmI    BpuEI     BsaI    BsaHI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI Bsp1286I    BspMI    BsrBI    BsrFI    BssSI   BstAPI
   BstEII    BstXI    BstYI   Bsu36I     BtgI     BtsI     DrdI     EaeI
     EagI     EarI  EcoICRI  Eco57MI EcoO109I     FalI    HaeII    Hin4I
   HincII     KasI     MscI     MslI   MspA1I     NarI     NheI     PasI
    PflMI     PfoI     PpiI    PpuMI     PsiI    PspXI     PstI    PvuII
     SacI     SalI    SanDI     SapI     SfcI     SfoI     SmlI     StyI
    TaqII     TstI     XhoI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AleI
     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BbsI    BbvCI    BciVI     BclI    BfrBI
    BmgBI     BplI   Bpu10I    BsaAI    BsaBI    BsaWI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BsrDI    BsrGI   BssHII    BstBI  BstZ17I
    BtgZI     ClaI    CspCI     DraI   DraIII     EciI    EcoNI    EcoRI
    EcoRV     FseI     FspI    FspAI  HindIII     HpaI     KpnI     MfeI
     MluI     MmeI     NaeI     NcoI     NdeI   NgoMIV     NotI     NruI
     NsiI     NspI     PacI     PciI     PmeI     PmlI    PshAI   PspOMI
     PsrI     PvuI    RsrII    SacII     SbfI     ScaI    SexAI     SfiI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI     TatI  Tth111I     XbaI     XcmI     XmaI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com