Restriction map for pF1KE0431
##################################
# NO CUT ENZYMES (  147 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AgeI        AhdI        Alw44I      AocI        Aor51HI     
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AspI        AvaI        AviII       AvrII       
BamHI       BbeI        BbrPI       BbvCI       BcgI        
BciVI       BclI        BcnI        BglII       BlnI        
BlpI        BpmI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BsgI        
BsiWI       BsmBI       BsmFI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       BstAPI      BstEII      BstPI       BstYI       
Bsu36I      CelII       Cfr10I      ClaI        CpoI        
DraIII      DrdI        Eam1105I    Eco47III    Eco81I      
EcoO65I     EcoT22I     FbaI        FseI        FspI        
HapII       HgaI        Hin1I       HincII      HindII      
HpaI        HpaII       KasI        KpnI        KspI        
MamI        MfeI        MflI        MluI        MroI        
MspA1I      MspI        MunI        NaeI        NarI        
NciI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
StuI        SwaI        Tsp45I      TspRI       Tth111I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AccI          456  GTATAC               GT^[AC][GT]AC
AflII         843  CTTAAG               C^TTAAG
AflIII        313  ACATGT               A^C[GA][CT]GT
AlwI          409  GATCC                GGATC(4/5)
AlwNI         524  CAGAATCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI            5  GGGCCC               GGGCC^C
AvaII          18  GGACC                G^G[AT]CC
BalI          863  TGGCCA               TGG^CCA
BanI          763  GGTGCC               G^G[CT][GA]CC
BanII           5  GGGCCC               G[GA]GC[CT]^C
BbvI          238  GCTGC                GCAGC(8/12)
BfaI          628  CTAG                 C^TAG
BfrI          843  CTTAAG               C^TTAAG
BglI           70  GCCCTGGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BseRI         233  GAGGAG               GAGGAG(10/8)
BsiEI          26  CGGCCG               CG[GA][CT]^CG
BspLU11I      313  ACATGT               A^CATGT
BsrI          706  ACTGG                ACTGG(1/-1)
BssKI          72  CCTGG                ^CC[ACGT]GG
Bst1107I      456  GTATAC               GTA^TAC
BstBI         179  TTCGAA               TT^CGAA
BstNI          72  CCTGG                CC^[AT]GG
BstXI         683  CCAATTTTATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I       456  GTATAC               GTA^TAC
DraI          724  TTTAAA               TTT^AAA
EagI           26  CGGCCG               C^GGCCG
EclXI          26  CGGCCG               C^GGCCG
Eco52I         26  CGGCCG               C^GGCCG
EcoNI         110  CCTTGAAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         160  GAATTC               G^AATTC
EcoRII         72  CCTGG                ^CC[AT]GG
EcoRV         893  GATATC               GAT^ATC
FauI           79  GCGGG                CCCGC(4/6)
HaeII          55  AGCGCC               [GA]GCGC^[CT]
HindIII       488  AAGCTT               A^AGCTT
HphI          107  TCACC                GGTGA(8/7)
MaeI          628  CTAG                 C^TAG
MaeII         533  ACGT                 A^CGT
MluNI         863  TGGCCA               TGG^CCA
MscI          863  TGGCCA               TGG^CCA
MvaI           72  CCTGG                CC^[AT]GG
NcoI           14  CCATGG               C^CATGG
NspI          313  ACATGT               [GA]CATG^[CT]
NspV          179  TTCGAA               TT^CGAA
ScrFI          72  CCTGG                CC^[ACGT]GG
SfcI          422  CTGTAG               C^T[GA][CT]AG
SfuI          179  TTCGAA               TT^CGAA
TseI          238  GCTGC                G^C[AT]GC


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AcsI          160  GAATTC               [GA]^AATT[CT]
              558  GAATTT              
ApoI          160  GAATTC               [GA]^AATT[CT]
              558  GAATTT              
Asp700I       634  GAAGTTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              733  GAACTAATTC          
AspHI         712  GTGCTC               G[AT]GC[AT]^C
              874  GAGCAC              
BbsI          120  GAAGAC               GAAGAC(2/6)
              132  GAAGAC              
BpuAI         120  GAAGAC               GAAGAC(2/6)
              132  GAAGAC              
BsiHKAI       712  GTGCTC               G[AT]GC[AT]^C
              874  GAGCAC              
BsmAI         827  GAGAC                GTCTC(1/5)
              839  GAGAC               
DdeI           95  CTCAG                C^T[ACGT]AG
              277  CTCAG               
DpnII         141  GATC                 ^GATC
              409  GATC                
DraII           4  AGGGCCC              [GA]G^G[ACGT]CC[CT]
                5  GGGCCCC             
DsaI           14  CCATGG               C^C[GA][CT]GG
               29  CCGTGG              
EaeI           26  CGGCCG               [CT]^GGCC[GA]
              863  TGGCCA              
EarI          114  GAAGAG               CTCTTC(1/4)
              427  GAAGAG              
EcoO109I        4  AGGGCCC              [GA]G^G[ACGT]CC[CT]
                5  GGGCCCC             
EcoT14I        14  CCATGG               C^C[AT][AT]GG
              834  CCAAGG              
Fnu4HI         25  GCGGC                GC^[ACGT]GC
              238  GCTGC               
ItaI           25  GCGGC                GC^[ACGT]GC
              238  GCTGC               
Ksp632I       114  GAAGAG               CTCTTC(1/4)
              427  GAAGAG              
MaeIII        346  GTTAC                ^GT[ACGT]AC
              809  GTAAC               
MboI          141  GATC                 ^GATC
              409  GATC                
NdeII         141  GATC                 ^GATC
              409  GATC                
PleI          829  GACTC                GAGTC(4/5)
              937  GACTC               
Sau3AI        141  GATC                 ^GATC
              409  GATC                
SmlI          768  CTTGAG               C^T[CT][GA]AG
              843  CTTAAG              
SspI          389  AATATT               AAT^ATT
              782  AATATT              
StyI           14  CCATGG               C^C[AT][AT]GG
              834  CCAAGG              
TaqI          146  TCGA                 T^CGA
              180  TCGA                
TthHB8I       146  TCGA                 T^CGA
              180  TCGA                
XmnI          634  GAAGTTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              733  GAACTAATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   33 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        BmyI        
BsaJI       BsiYI       BslI        Bsp1286I    BstF5I      
BstUI       Cac8I       CfoI        Cfr13I      FokI        
HaeIII      HhaI        HinP1I      HinfI       MboII       
MnlI        MseI        MslI        MvnI        MwoI        
NlaIII      NlaIV       RsaI        Sau96I      SfaNI       
TfiI        Tru9I       Tsp509I     
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