Restriction map for pF1KE0431
 (Linear) (Six-base) MAPSORT of: KIEE0431.seq  Check: 7648  from: 1  to: 1008



KIEE0431.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:14  ..

AccI GT'mk_AC

Cuts at:      0     457    1008 
   Size:        457     551
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     232    1008 
   Size:        232     776
 
AflII C'TTAA_G

Cuts at:      0     843    1008 
   Size:        843     165
 
AflIII A'CryG_T

Cuts at:      0     313    1008 
   Size:        313     695
 
AlwNI CAG_nnn'CTG

Cuts at:      0     529    1008 
   Size:        529     479
 
ApaI G_GGCC'C

Cuts at:      0       9    1008 
   Size:          9     999
 
ApoI r'AATT_y

Cuts at:      0     160     558    1008 
   Size:        160     398     450

  Fragments arranged by size: 

                 450     398     160
 
BanI G'GyrC_C

Cuts at:      0     763    1008 
   Size:        763     245
 
BanII G_rGCy'C

Cuts at:      0       9    1008 
   Size:          9     999
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     127     139    1008 
   Size:        127      12     869

  Fragments arranged by size: 

                 869     127      12
 
BglI GCCn_nnn'nGGC

Cuts at:      0      76    1008 
   Size:         76     932
 
Bme1580I G_kGCm'C

Cuts at:      0       9    1008 
   Size:          9     999
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     716    1008 
   Size:        716     292
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     789    1008 
   Size:        789     219
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     248    1008 
   Size:        248     760
 
BsiEI CG_ry'CG

Cuts at:      0      29    1008 
   Size:         29     979
 
BsiHKAI G_wGCw'C

Cuts at:      0     716     878    1008 
   Size:        716     162     130

  Fragments arranged by size: 

                 716     162     130
 
Bsp1286I G_dGCh'C

Cuts at:      0       9     716     878    1008 
   Size:          9     707     162     130

  Fragments arranged by size: 

                 707     162     130       9
 
BstBI TT'CG_AA

Cuts at:      0     180    1008 
   Size:        180     828
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     690    1008 
   Size:        690     318
 
BstZ17I GTA'TAC

Cuts at:      0     458    1008 
   Size:        458     550
 
BtgI C'CryG_G

Cuts at:      0      14      29    1008 
   Size:         14      15     979

  Fragments arranged by size: 

                 979      15      14
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     635    1008 
   Size:        635     373
 
DraI TTT'AAA

Cuts at:      0     726    1008 
   Size:        726     282
 
EaeI y'GGCC_r

Cuts at:      0      26     863    1008 
   Size:         26     837     145

  Fragments arranged by size: 

                 837     145      26
 
EagI C'GGCC_G

Cuts at:      0      26    1008 
   Size:         26     982
 
EarI CTCTTCn'nnn_

Cuts at:      0     109     422    1008 
   Size:        109     313     586

  Fragments arranged by size: 

                 586     313     109
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     144    1008 
   Size:        144     864
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     232    1008 
   Size:        232     776
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     114    1008 
   Size:        114     894
 
EcoO109I rG'GnC_Cy

Cuts at:      0       5       6    1008 
   Size:          5       1    1002

  Fragments arranged by size: 

                1002       5       1
 
EcoRI G'AATT_C

Cuts at:      0     160    1008 
   Size:        160     848
 
EcoRV GAT'ATC

Cuts at:      0     895    1008 
   Size:        895     113
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     523    1008 
   Size:        523     485
 
HaeII r_GCGC'y

Cuts at:      0      59    1008 
   Size:         59     949
 
HindIII A'AGCT_T

Cuts at:      0     488    1008 
   Size:        488     520
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     600    1008 
   Size:        600     408
 
MscI TGG'CCA

Cuts at:      0     865    1008 
   Size:        865     143
 
MslI CAynn'nnrTG

Cuts at:      0     148     592     665     761    1008 
   Size:        148     444      73      96     247

  Fragments arranged by size: 

                 444     247     148      96      73
 
NcoI C'CATG_G

Cuts at:      0      14    1008 
   Size:         14     994
 
NspI r_CATG'y

Cuts at:      0     317    1008 
   Size:        317     691
 
PciI A'CATG_T

Cuts at:      0     313    1008 
   Size:        313     695
 
PspOMI G'GGCC_C

Cuts at:      0       5    1008 
   Size:          5    1003
 
SfcI C'TryA_G

Cuts at:      0     422    1008 
   Size:        422     586
 
SmlI C'TyrA_G

Cuts at:      0     768     843    1008 
   Size:        768      75     165

  Fragments arranged by size: 

                 768     165      75
 
SspI AAT'ATT

Cuts at:      0     391     784    1008 
   Size:        391     393     224

  Fragments arranged by size: 

                 393     391     224
 
StyI C'CwwG_G

Cuts at:      0      14     834    1008 
   Size:         14     820     174

  Fragments arranged by size: 

                 820     174      14
 
TatI w'GTAC_w

Cuts at:      0      98     624     929     957    1008 
   Size:         98     526     305      28      51

  Fragments arranged by size: 

                 526     305      98      51      28
 
XmnI GAAnn'nnTTC

Cuts at:      0     638     737    1008 
   Size:        638      99     271

  Fragments arranged by size: 

                 638     271      99
 

 Enzymes that do cut:

     AccI     AcuI    AflII   AflIII    AlwNI     ApaI     ApoI     BanI
    BanII     BbsI     BglI Bme1580I     BmrI    BpuEI    BseRI    BsiEI
  BsiHKAI Bsp1286I    BstBI    BstXI  BstZ17I     BtgI    BtgZI     DraI
     EaeI     EagI     EarI     EciI  Eco57MI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI    HaeII  HindIII     MmeI     MscI     MslI     NcoI
     NspI     PciI   PspOMI     SfcI     SmlI     SspI     StyI     TatI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI     AgeI     AhdI     AleI
     AlfI     AloI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI    BamHI     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI
    BglII     BlpI    BmgBI     BmtI     BplI     BpmI   Bpu10I     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI   BstEII    BstYI   Bsu36I     BtsI
     ClaI    CspCI   DraIII     DrdI  EcoICRI     FseI     FspI    FspAI
    Hin4I   HincII     HpaI     KasI     KpnI     MfeI     MluI   MspA1I
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     StuI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com