Restriction map for
pF1KB7759
(Circular) (Six-base) MAPSORT of: pF1KB7759.seq Check: 5940 from: 1 to: 4454
pF1KB7759 4454 bp
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AccI GT'mk_AC
Cuts at: 1492 1492
Size: 4454
Acc65I G'GTAC_C
Cuts at: 1470 1470
Size: 4454
AclI AA'CG_TT
Cuts at: 4294 4294
Size: 4454
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 311 655 664 997 2279 2711 3458 3726 311
Size: 344 9 333 1282 432 747 268 1039
Fragments arranged by size:
1282 1039 747 432 344 333 268 9
AfeI AGC'GCT
Cuts at: 865 865
Size: 4454
AflIII A'CryG_T
Cuts at: 1806 2910 3887 1806
Size: 1104 977 2373
Fragments arranged by size:
2373 1104 977
AgeI A'CCGG_T
Cuts at: 2842 2842
Size: 4454
AleI CACnn'nnGTG
Cuts at: 1261 1261
Size: 4454
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 884 1400 884
Size: 516 3938
AlwNI CAG_nnn'CTG
Cuts at: 218 1759 3326 218
Size: 1541 1567 1346
Fragments arranged by size:
1567 1541 1346
ApaI G_GGCC'C
Cuts at: 129 129
Size: 4454
ApaLI G'TGCA_C
Cuts at: 1441 3224 1441
Size: 1783 2671
ApoI r'AATT_y
Cuts at: 1108 1458 3807 4063 1108
Size: 350 2349 256 1499
Fragments arranged by size:
2349 1499 350 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4454
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4454
AvaI C'yCGr_G
Cuts at: 423 1474 423
Size: 1051 3403
BamHI G'GATC_C
Cuts at: 1479 1479
Size: 4454
BanI G'GyrC_C
Cuts at: 828 1470 1884 2132 2167 828
Size: 642 414 248 35 3115
Fragments arranged by size:
3115 642 414 248 35
BanII G_rGCy'C
Cuts at: 129 296 323 1468 2498 129
Size: 167 27 1145 1030 2085
Fragments arranged by size:
2085 1145 1030 167 27
BbeI G_GCGC'C
Cuts at: 1888 2136 1888
Size: 248 4206
BbsI GAAGACnn'nnnn_
Cuts at: 985 1085 985
Size: 100 4354
BciVI GTATCCnnnnn_n'
Cuts at: 2352 3113 4454 2352
Size: 761 1341 2352
Fragments arranged by size:
2352 1341 761
BclI T'GATC_A
Cuts at: 1974 1974
Size: 4454
BglI GCCn_nnn'nGGC
Cuts at: 515 515
Size: 4454
BglII A'GATC_T
Cuts at: 1430 1969 4006 1430
Size: 539 2037 1878
Fragments arranged by size:
2037 1878 539
BlpI GC'TnA_GC
Cuts at: 724 949 1566 724
Size: 225 617 3612
Fragments arranged by size:
3612 617 225
Bme1580I G_kGCm'C
Cuts at: 129 1445 2079 2172 3228 129
Size: 1316 634 93 1056 1355
Fragments arranged by size:
1355 1316 1056 634 93
BmrI ACTGGGnnnn_n'
Cuts at: 748 1666 1747 2082 4226 748
Size: 918 81 335 2144 976
Fragments arranged by size:
2144 976 918 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2821 2821
Size: 4454
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 301 1027 1119 1219 301
Size: 726 92 100 3536
Fragments arranged by size:
3536 726 100 92
Bpu10I CC'TnA_GC
Cuts at: 1357 1357
Size: 4454
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1039 1630 2509 3001 3299 1039
Size: 591 879 492 298 2194
Fragments arranged by size:
2194 879 591 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4158 4158
Size: 4454
BsaAI yAC'GTr
Cuts at: 1807 1807
Size: 4454
BsaBI GATnn'nnATC
Cuts at: 6 1968 4005 6
Size: 1962 2037 455
Fragments arranged by size:
2037 1962 455
BsaHI Gr'CG_yC
Cuts at: 1885 2133 2835 4331 1885
Size: 248 702 1496 2008
Fragments arranged by size:
2008 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1349 1643 2164 2842 3116 3263 3674 1349
Size: 294 521 678 274 147 411 2129
Fragments arranged by size:
2129 678 521 411 294 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 772 802 1026 1056 772
Size: 30 224 30 4170
Fragments arranged by size:
4170 224 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 616 670 616
Size: 54 4400
BseYI C'CCAG_C
Cuts at: 120 256 384 719 767 1879 2637 3214 3710
Size: 136 128 335 48 1112 758 577 496
Cuts at: 3710 120
Size: 864
Fragments arranged by size:
1112 864 758 577 496 335 136 128
48
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 922 1243 922
Size: 321 4133
BsiEI CG_ry'CG
Cuts at: 104 992 1996 2042 3250 104
Size: 888 1004 46 1208 1308
Fragments arranged by size:
1308 1208 1004 888 46
BsiHKAI G_wGCw'C
Cuts at: 1445 1468 2246 2436 3228 1445
Size: 23 778 190 792 2671
Fragments arranged by size:
2671 792 778 190 23
BsmBI CGTCTCn'nnnn_
Cuts at: 900 1290 900
Size: 390 4064
Bsp1286I G_dGCh'C
Cuts at: 129 296 323 1445 1468 2079 2172 2246 2436
Size: 167 27 1122 23 611 93 74 190
Cuts at: 2436 2498 3228 129
Size: 62 730 1355
Fragments arranged by size:
1355 1122 730 611 190 167 93 74
62 27 23
BspEI T'CCGG_A
Cuts at: 1349 3674 1349
Size: 2325 2129
BspHI T'CATG_A
Cuts at: 3630 3630
Size: 4454
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1504 1718 2020 2401 1504
Size: 214 302 381 3557
Fragments arranged by size:
3557 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2746 2800 4284 4449 50
Size: 2696 54 1484 165 55
Fragments arranged by size:
2696 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1184 2366 1184
Size: 1182 3272
BsrFI r'CCGG_y
Cuts at: 782 800 1211 2452 2633 2842 782
Size: 18 411 1241 181 209 2394
Fragments arranged by size:
2394 1241 411 209 181 18
BssSI C'ACGA_G
Cuts at: 2725 3083 2725
Size: 358 4096
BstAPI GCAn_nnn'nTGC
Cuts at: 187 218 1831 187
Size: 31 1613 2810
Fragments arranged by size:
2810 1613 31
BstBI TT'CG_AA
Cuts at: 2815 2815
Size: 4454
BstXI CCAn_nnnn'nTGG
Cuts at: 256 384 256
Size: 128 4326
BstYI r'GATC_y
Cuts at: 1 341 626 834 1430 1479 1946 1969 2304
Size: 340 285 208 596 49 467 23 335
Cuts at: 2304 2550 3562 3648 3660 4006 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 596 467 449 346 340 335 285
246 208 86 49 23 12
BtgI C'CryG_G
Cuts at: 530 824 1321 530
Size: 294 497 3663
Fragments arranged by size:
3663 497 294
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2404 2585 2748 2404
Size: 181 163 4110
Fragments arranged by size:
4110 181 163
BtsI GCAGTG_nn'
Cuts at: 522 1387 1697 522
Size: 865 310 3279
Fragments arranged by size:
3279 865 310
DraI TTT'AAA
Cuts at: 1453 1453
Size: 4454
DrdI GACnn_nn'nnGTC
Cuts at: 2160 3018 2160
Size: 858 3596
EaeI y'GGCC_r
Cuts at: 519 887 2039 2213 2604 2631 3976 519
Size: 368 1152 174 391 27 1345 997
Fragments arranged by size:
1345 1152 997 391 368 174 27
EagI C'GGCC_G
Cuts at: 2039 2039
Size: 4454
EarI CTCTTCn'nnn_
Cuts at: 2477 2687 2477
Size: 210 4244
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 463 619 2662 2972 3118 4264 463
Size: 156 2043 310 146 1146 653
Fragments arranged by size:
2043 1146 653 310 156 146
EcoICRI GAG'CTC
Cuts at: 1466 1466
Size: 4454
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 301 311 655 664 997 1027 1119 1219 2279
Size: 10 344 9 333 30 92 100 1060
Cuts at: 2279 2711 3458 3726 301
Size: 432 747 268 1029
Fragments arranged by size:
1060 1029 747 432 344 333 268 100
92 30 10 9
EcoNI CCTnn'n_nnAGG
Cuts at: 229 229
Size: 4454
EcoO109I rG'GnC_Cy
Cuts at: 125 126 1593 125
Size: 1 1467 2986
Fragments arranged by size:
2986 1467 1
EcoRI G'AATT_C
Cuts at: 1108 1458 1108
Size: 350 4104
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 486 2473 486
Size: 1987 2467
FspI TGC'GCA
Cuts at: 2235 2235
Size: 4454
HaeII r_GCGC'y
Cuts at: 789 867 1888 2136 3158 4137 789
Size: 78 1021 248 1022 979 1106
Fragments arranged by size:
1106 1022 1021 979 248 78
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 406 438 2296 2326 2328 2358 406
Size: 32 1858 30 2 30 2502
Fragments arranged by size:
2502 1858 32 30 30 2
HincII GTy'rAC
Cuts at: 915 1493 915
Size: 578 3876
KasI G'GCGC_C
Cuts at: 1884 2132 1884
Size: 248 4206
KpnI G_GTAC'C
Cuts at: 1474 1474
Size: 4454
MluI A'CGCG_T
Cuts at: 3887 3887
Size: 4454
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3125 3309 3125
Size: 184 4270
MscI TGG'CCA
Cuts at: 889 2215 889
Size: 1326 3128
MslI CAynn'nnrTG
Cuts at: 437 541 1259 1261 1301 2443 2570 437
Size: 104 718 2 40 1142 127 2321
Fragments arranged by size:
2321 1142 718 127 104 40 2
MspA1I CmG'CkG
Cuts at: 218 1419 1565 1879 2239 3252 3497 218
Size: 1201 146 314 360 1013 245 1175
Fragments arranged by size:
1201 1175 1013 360 314 245 146
NaeI GCC'GGC
Cuts at: 2635 2635
Size: 4454
NarI GG'CG_CC
Cuts at: 1885 2133 1885
Size: 248 4206
NcoI C'CATG_G
Cuts at: 530 824 530
Size: 294 4160
NdeI CA'TA_TG
Cuts at: 1260 1260
Size: 4454
NgoMIV G'CCGG_C
Cuts at: 2633 2633
Size: 4454
NspI r_CATG'y
Cuts at: 546 597 1279 1507 2007 2914 546
Size: 51 682 228 500 907 2086
Fragments arranged by size:
2086 907 682 500 228 51
PasI CC'CwG_GG
Cuts at: 204 689 1030 204
Size: 485 341 3628
Fragments arranged by size:
3628 485 341
PciI A'CATG_T
Cuts at: 2910 2910
Size: 4454
PmeI GTTT'AAAC
Cuts at: 1453 1453
Size: 4454
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3593 3625 3593
Size: 32 4422
PsiI TTA'TAA
Cuts at: 1070 1070
Size: 4454
PspOMI G'GGCC_C
Cuts at: 125 125
Size: 4454
PstI C_TGCA'G
Cuts at: 270 291 947 1209 1253 1400 1501 2186 270
Size: 21 656 262 44 147 101 685 2538
Fragments arranged by size:
2538 685 656 262 147 101 44 21
PvuI CG_AT'CG
Cuts at: 104 992 104
Size: 888 3566
PvuII CAG'CTG
Cuts at: 218 1879 2239 218
Size: 1661 360 2433
Fragments arranged by size:
2433 1661 360
RsrII CG'GwC_CG
Cuts at: 2649 2649
Size: 4454
SacI G_AGCT'C
Cuts at: 1468 1468
Size: 4454
SalI G'TCGA_C
Cuts at: 1491 1491
Size: 4454
SapI GCTCTTCn'nnn_
Cuts at: 2477 2687 2477
Size: 210 4244
SbfI CC_TGCA'GG
Cuts at: 1501 1501
Size: 4454
ScaI AGT'ACT
Cuts at: 3971 3971
Size: 4454
SfcI C'TryA_G
Cuts at: 33 266 287 943 1205 1249 1396 1497 2182
Size: 233 21 656 262 44 147 101 685
Cuts at: 2182 3175 3366 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 685 656 262 233 191 147
101 44 21
SfoI GGC'GCC
Cuts at: 1886 2134 1886
Size: 248 4206
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4454
SmaI CCC'GGG
Cuts at: 1476 1476
Size: 4454
SmlI C'TyrA_G
Cuts at: 1054 1609 2524 3016 3278 1054
Size: 555 915 492 262 2230
Fragments arranged by size:
2230 915 555 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4454
SphI G_CATG'C
Cuts at: 597 1279 1507 2007 597
Size: 682 228 500 3044
Fragments arranged by size:
3044 682 500 228
StuI AGG'CCT
Cuts at: 421 421
Size: 4454
StyI C'CwwG_G
Cuts at: 530 817 824 1588 1942 530
Size: 287 7 764 354 3042
Fragments arranged by size:
3042 764 354 287 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1322 2037 2170 2629 2838 1322
Size: 715 133 459 209 2938
Fragments arranged by size:
2938 715 459 209 133
TatI w'GTAC_w
Cuts at: 3969 3969
Size: 4454
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3971 4003 3971
Size: 32 4422
Tth111I GACn'n_nGTC
Cuts at: 2251 2251
Size: 4454
XbaI T'CTAG_A
Cuts at: 1485 1485
Size: 4454
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1949 1949
Size: 4454
XmaI C'CCGG_G
Cuts at: 1474 1474
Size: 4454
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI
PasI PciI PmeI PpiI PsiI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AloI AscI AvrII BaeI
BbvCI BcgI BfrBI BmgBI BmtI BsiWI BsmI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NheI NotI NruI
NsiI PacI PflMI PfoI PmlI PpuMI PshAI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI XmnI ZraI