Restriction map for pF1KB4465
 (Circular) (Six-base) MAPSORT of: pF1KB4465.seq  Check: 7388  from: 1  to: 10076



pF1KB4465  10076 bp

 With 182 enzymes: SgfI * 

                        May 1, 2012 15:29  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    634     748    1099    1316    1597    2392    2623    2842    2848 
   Size:        114     351     217     281     795     231     219       6

Cuts at:   2848    3076    3658    6648     634 
   Size:        228     582    2990    4062

  Fragments arranged by size: 

                4062    2990     795     582     351     281     231     228
                 219     217     114       6
 
AatII G_ACGT'C

Cuts at:   2409    2409 
   Size:      10076
 
AccI GT'mk_AC

Cuts at:   7114    7114 
   Size:      10076
 
Acc65I G'GTAC_C

Cuts at:    973    7092     973 
   Size:       6119    3957
 
AclI AA'CG_TT

Cuts at:   9916    9916 
   Size:      10076
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1563    1587    5552    7901    8333    9080    9348    1563 
   Size:         24    3965    2349     432     747     268    2291

  Fragments arranged by size: 

                3965    2349    2291     747     432     268      24
 
AfeI AGC'GCT

Cuts at:   4306    4306 
   Size:      10076
 
AflIII A'CryG_T

Cuts at:   1615    3425    7428    8532    9509    1615 
   Size:       1810    4003    1104     977    2182

  Fragments arranged by size: 

                4003    2182    1810    1104     977
 
AgeI A'CCGG_T

Cuts at:   8464    8464 
   Size:      10076
 
AleI CACnn'nnGTG

Cuts at:   1069    1948    3652    4487    6508    1069 
   Size:        879    1704     835    2021    4637

  Fragments arranged by size: 

                4637    2021    1704     879     835
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    295    3018    4750    5660    5749     295 
   Size:       2723    1732     910      89    4622

  Fragments arranged by size: 

                4622    2723    1732     910      89
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5012    5044    5012 
   Size:         32   10044
 
AlwNI CAG_nnn'CTG

Cuts at:   2304    2880    2903    3105    4430    5437    6931    7381    8948 
   Size:        576      23     202    1325    1007    1494     450    1567

Cuts at:   8948    2304 
   Size:       3432

  Fragments arranged by size: 

                3432    1567    1494    1325    1007     576     450     202
                  23
 
ApaI G_GGCC'C

Cuts at:    123     521    1424    2063    3091    3629    4815    6197    6338 
   Size:        398     903     639    1028     538    1186    1382     141

Cuts at:   6338    6379    6449    6485    6627    7034     123 
   Size:         41      70      36     142     407    3165

  Fragments arranged by size: 

                3165    1382    1186    1028     903     639     538     407
                 398     142     141      70      41      36
 
ApaLI G'TGCA_C

Cuts at:   8846    8846 
   Size:      10076
 
ApoI r'AATT_y

Cuts at:   7080    9429    9685    7080 
   Size:       2349     256    7471

  Fragments arranged by size: 

                7471    2349     256
 
AseI AT'TA_AT

Cuts at:     20    3584      20 
   Size:       3564    6512
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:      10076
 
AvaI C'yCGr_G

Cuts at:    287    1596    2190    2309    2799    3073    3764    3900    4089 
   Size:       1309     594     119     490     274     691     136     189

Cuts at:   4089    4243    4808    4831    6307    6338    6372    7096     287 
   Size:        154     565      23    1476      31      34     724    3267

  Fragments arranged by size: 

                3267    1476    1309     724     691     594     565     490
                 274     189     154     136     119      34      31      23
            
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:   3687    3720    3687 
   Size:         33   10043
 
BamHI G'GATC_C

Cuts at:   7101    7101 
   Size:      10076
 
BanI G'GyrC_C

Cuts at:    406     428     499     622     702     736     973    1300    1663 
   Size:         22      71     123      80      34     237     327     363

Cuts at:   1663    2803    3632    3646    3742    4227    4239    4265    4409 
   Size:       1140     829      14      96     485      12      26     144

Cuts at:   4409    4617    5394    6554    6675    7092    7506    7754    7789 
   Size:        208     777    1160     121     417     414     248      35

Cuts at:   7789     406 
   Size:       2693

  Fragments arranged by size: 

                2693    1160    1140     829     777     485     417     414
                 363     327     248     237     208     144     123     121
                  96      80      71      35      34      26      22      14
                  12
 
BanII G_rGCy'C

Cuts at:    123     521     608    1348    1424    2063    2216    2716    2979 
   Size:        398      87     740      76     639     153     500     263

Cuts at:   2979    3091    3629    4153    4815    5514    5595    5882    6197 
   Size:        112     538     524     662     699      81     287     315

Cuts at:   6197    6338    6379    6449    6485    6627    7034    7090    8120 
   Size:        141      41      70      36     142     407      56    1030

Cuts at:   8120     123 
   Size:       2079

  Fragments arranged by size: 

                2079    1030     740     699     662     639     538     524
                 500     407     398     315     287     263     153     142
                 141     112      87      81      76      70      56      41
                  36
 
BbeI G_GCGC'C

Cuts at:   1304    1667    4231    4413    6558    6679    7510    7758    1304 
   Size:        363    2564     182    2145     121     831     248    3622

  Fragments arranged by size: 

                3622    2564    2145     831     363     248     182     121
            
 
BbsI GAAGACnn'nnnn_

Cuts at:    772    3305    3430    4764    5760     772 
   Size:       2533     125    1334     996    5088

  Fragments arranged by size: 

                5088    2533    1334     996     125
 
BbvCI CC'TCA_GC

Cuts at:    478    5330     478 
   Size:       4852    5224
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    404     438    1042    1076    3054    3088    3507    3541    4525 
   Size:         34     604      34    1978      34     419      34     984

Cuts at:   4525    4559    5416    5450     404 
   Size:         34     857      34    5030

  Fragments arranged by size: 

                5030    1978     984     857     604     419      34      34
                  34      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3688    7974    8735   10076    3688 
   Size:       4286     761    1341    3688

  Fragments arranged by size: 

                4286    3688    1341     761
 
BclI T'GATC_A

Cuts at:   4899    7596    4899 
   Size:       2697    7379
 
BfrBI ATG'CAT

Cuts at:   3518    3518 
   Size:      10076
 
BglI GCCn_nnn'nGGC

Cuts at:    314     437     551     620    1133    1598    1886    1889    2015 
   Size:        123     114      69     513     465     288       3     126

Cuts at:   2015    2048    2105    2162    2507    2705    2849    2963    3329 
   Size:         33      57      57     345     198     144     114     366

Cuts at:   3329    4007    4035    4285    4630    5471    5815    5854    5974 
   Size:        678      28     250     345     841     344      39     120

Cuts at:   5974    6333    6342    6940     314 
   Size:        359       9     598    3450

  Fragments arranged by size: 

                3450     841     678     598     513     465     366     359
                 345     345     344     288     250     198     144     126
                 123     120     114     114      69      57      57      39
                  33      28       9       3
 
BglII A'GATC_T

Cuts at:   7591    9628    7591 
   Size:       2037    8039
 
BlpI GC'TnA_GC

Cuts at:   4363    4711    5594    7188    4363 
   Size:        348     883    1594    7251

  Fragments arranged by size: 

                7251    1594     883     348
 
Bme1580I G_kGCm'C

Cuts at:    123     310     433     495     504     521     707     739     895 
   Size:        187     123      62       9      17     186      32     156

Cuts at:    895    1424    1726    1837    2063    2208    2806    3091    3325 
   Size:        529     302     111     226     145     598     285     234

Cuts at:   3325    3629    3942    4003    4270    4815    5287    5607    6197 
   Size:        304     313      61     267     545     472     320     590

Cuts at:   6197    6338    6379    6449    6485    6614    6627    6786    6966 
   Size:        141      41      70      36     129      13     159     180

Cuts at:   6966    7034    7701    7794    8850     123 
   Size:         68     667      93    1056    1349

  Fragments arranged by size: 

                1349    1056     667     598     590     545     529     472
                 320     313     304     302     285     267     234     226
                 187     186     180     159     156     145     141     129
                 123     111      93      70      68      62      61      41
                  36      32      17      13       9
 
BmgBI CAC'GTC

Cuts at:    582     930    1629    3474    4992    5163     582 
   Size:        348     699    1845    1518     171    5495

  Fragments arranged by size: 

                5495    1845    1518     699     348     171
 
BmrI ACTGGGnnnn_n'

Cuts at:   3170    5949    6182    6818    6879    6947    6968    7288    7369 
   Size:       2779     233     636      61      68      21     320      81

Cuts at:   7369    7704    9848    3170 
   Size:        335    2144    3398

  Fragments arranged by size: 

                3398    2779    2144     636     335     320     233      81
                  68      61      21
 
BmtI G_CTAG'C

Cuts at:    208    5564     208 
   Size:       5356    4720
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3504    3753    5163    8443    3504 
   Size:        249    1410    3280    5137

  Fragments arranged by size: 

                5137    3280    1410     249
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    361    2298    3280    4879    5000    5029    5536    6227    6587 
   Size:       1937     982    1599     121      29     507     691     360

Cuts at:   6587    6632     361 
   Size:         45    3805

  Fragments arranged by size: 

                3805    1937    1599     982     691     507     360     121
                  45      29
 
Bpu10I CC'TnA_GC

Cuts at:    478    2782    3738    5330    6863    7010     478 
   Size:       2304     956    1592    1533     147    3544

  Fragments arranged by size: 

                3544    2304    1592    1533     956     147
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   6496    7252    8131    8623    8921    6496 
   Size:        756     879     492     298    7651

  Fragments arranged by size: 

                7651     879     756     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   1387    4254    6019    9780    1387 
   Size:       2867    1765    3761    1683

  Fragments arranged by size: 

                3761    2867    1765    1683
 
BsaAI yAC'GTr

Cuts at:   1442    1616    2820    7429    1442 
   Size:        174    1204    4609    4089

  Fragments arranged by size: 

                4609    4089    1204     174
 
BsaBI GATnn'nnATC

Cuts at:      6    5779    7590    9627       6 
   Size:       5773    1811    2037     455

  Fragments arranged by size: 

                5773    2037    1811     455
 
BsaHI Gr'CG_yC

Cuts at:    869    1166    1301    1664    2315    2406    3032    3198    4010 
   Size:        297     135     363     651      91     626     166     812

Cuts at:   4010    4027    4228    4410    6555    6676    7507    7755    8457 
   Size:         17     201     182    2145     121     831     248     702

Cuts at:   8457    9953     869 
   Size:       1496     992

  Fragments arranged by size: 

                2145    1496     992     831     812     702     651     626
                 363     297     248     201     182     166     135     121
                  91      17
 
BsaWI w'CCGG_w

Cuts at:   1215    7265    7786    8464    8738    8885    9296    1215 
   Size:       6050     521     678     274     147     411    1995

  Fragments arranged by size: 

                6050    1995     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1230    1260    1738    1768    2168    2198    2809    2839    3253 
   Size:         30     478      30     400      30     611      30     414

Cuts at:   3253    3283    3982    4012    4135    4165    4249    4279    6560 
   Size:         30     699      30     123      30      84      30    2281

Cuts at:   6560    6590    1230 
   Size:         30    4716

  Fragments arranged by size: 

                4716    2281     699     611     478     414     400     123
                  84      30      30      30      30      30      30      30
                  30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   2410    4270    4594    4687    5326    5347    6037    6778    2410 
   Size:       1860     324      93     639      21     690     741    5708

  Fragments arranged by size: 

                5708    1860     741     690     639     324      93      21
            
 
BseYI C'CCAG_C

Cuts at:    275     317     990    2327    2896    3377    3491    3564    3863 
   Size:         42     673    1337     569     481     114      73     299

Cuts at:   3863    3941    4340    4589    5023    5211    5689    6321    6686 
   Size:         78     399     249     434     188     478     632     365

Cuts at:   6686    6778    6924    6974    6997    7022    7026    7034    7501 
   Size:         92     146      50      23      25       4       8     467

Cuts at:   7501    8259    8836    9332     275 
   Size:        758     577     496    1019

  Fragments arranged by size: 

                1337    1019     758     673     632     577     569     496
                 481     478     467     434     399     365     299     249
                 188     146     114      92      78      73      50      42
                  25      23       8       4
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    903    1569    1977    2355    2364    2415    2568    2595    2820 
   Size:        666     408     378       9      51     153      27     225

Cuts at:   2820    3048    3138    3663    5137    5719    6139     903 
   Size:        228      90     525    1474     582     420    4840

  Fragments arranged by size: 

                4840    1474     666     582     525     420     408     378
                 228     225     153      90      51      27       9
 
BsiEI CG_ry'CG

Cuts at:    104    1227    1368    1452    4130    4314    4554    7618    7664 
   Size:       1123     141      84    2678     184     240    3064      46

Cuts at:   7664    8872     104 
   Size:       1208    1308

  Fragments arranged by size: 

                3064    2678    1308    1208    1123     240     184     141
                  84      46
 
BsiHKAI G_wGCw'C

Cuts at:   1294    1319    1821    3575    4652    5120    5882    6833    6869 
   Size:         25     502    1754    1077     468     762     951      36

Cuts at:   6869    7090    7868    8058    8850    1294 
   Size:        221     778     190     792    2520

  Fragments arranged by size: 

                2520    1754    1077     951     792     778     762     502
                 468     221     190      36      25
 
BsmI GAATG_Cn'

Cuts at:   1642    1867    1642 
   Size:        225    9851
 
BsmBI CGTCTCn'nnnn_

Cuts at:   2306    6717    2306 
   Size:       4411    5665
 
Bsp1286I G_dGCh'C

Cuts at:    123     310     433     495     504     521     608     707     739 
   Size:        187     123      62       9      17      87      99      32

Cuts at:    739     895    1294    1319    1348    1424    1726    1821    1837 
   Size:        156     399      25      29      76     302      95      16

Cuts at:   1837    2063    2208    2216    2716    2806    2979    3091    3325 
   Size:        226     145       8     500      90     173     112     234

Cuts at:   3325    3575    3629    3942    4003    4153    4270    4652    4815 
   Size:        250      54     313      61     150     117     382     163

Cuts at:   4815    5120    5287    5514    5595    5607    5882    6197    6338 
   Size:        305     167     227      81      12     275     315     141

Cuts at:   6338    6379    6449    6485    6614    6627    6786    6833    6869 
   Size:         41      70      36     129      13     159      47      36

Cuts at:   6869    6966    7034    7090    7701    7794    7868    8058    8120 
   Size:         97      68      56     611      93      74     190      62

Cuts at:   8120    8850     123 
   Size:        730    1349

  Fragments arranged by size: 

                1349     730     611     500     399     382     315     313
                 305     302     275     250     234     227     226     190
                 187     173     167     163     159     156     150     145
                 141     129     123     117     112      99      97      95
                  93      90      87      81      76      74      70      68
                  62      62      61      56      54      47      41      36
                  36      32      29      25      17      16      13      12
                   9       8
 
BspEI T'CCGG_A

Cuts at:   9296    9296 
   Size:      10076
 
BspHI T'CATG_A

Cuts at:   9252    9252 
   Size:      10076
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    634     748     985    1099    1316    1597    2392    2623    2842 
   Size:        114     237     114     217     281     795     231     219

Cuts at:   2842    2848    2888    3076    3409    3658    6648    7126    7340 
   Size:          6      40     188     333     249    2990     478     214

Cuts at:   7340    7642    8023     634 
   Size:        302     381    2687

  Fragments arranged by size: 

                2990    2687     795     478     381     333     302     281
                 249     237     231     219     217     214     188     114
                 114      40       6
 
BsrBI CCG'CTC

Cuts at:     50     330    3194    3969    4170    5792    6726    8368    8422 
   Size:        280    2864     775     201    1622     934    1642      54

Cuts at:   8422    9906   10071      50 
   Size:       1484     165      55

  Fragments arranged by size: 

                2864    1642    1622    1484     934     775     280     201
                 165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   4393    7988    4393 
   Size:       3595    6481
 
BsrFI r'CCGG_y

Cuts at:    425    2341    2746    4486    4620    4784    4871    5183    8074 
   Size:       1916     405    1740     134     164      87     312    2891

Cuts at:   8074    8255    8464     425 
   Size:        181     209    2037

  Fragments arranged by size: 

                2891    2037    1916    1740     405     312     209     181
                 164     134      87
 
BssHII G'CGCG_C

Cuts at:   3976    4196    4272    3976 
   Size:        220      76    9780

  Fragments arranged by size: 

                9780     220      76
 
BssSI C'ACGA_G

Cuts at:   1189    2694    3526    8347    8705    1189 
   Size:       1505     832    4821     358    2560

  Fragments arranged by size: 

                4821    2560    1505     832     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1184    1652    1841    1991    2792    6970    7453    1184 
   Size:        468     189     150     801    4178     483    3807

  Fragments arranged by size: 

                4178    3807     801     483     468     189     150
 
BstBI TT'CG_AA

Cuts at:   8437    8437 
   Size:      10076
 
BstXI CCAn_nnnn'nTGG

Cuts at:   1674    1687    4489    6565    1674 
   Size:         13    2802    2076    5185

  Fragments arranged by size: 

                5185    2802    2076      13
 
BstYI r'GATC_y

Cuts at:      1     468    7101    7568    7591    7926    8172    9184    9270 
   Size:        467    6633     467      23     335     246    1012      86

Cuts at:   9270    9282    9628       1 
   Size:         12     346     449

  Fragments arranged by size: 

                6633    1012     467     467     449     346     335     246
                  86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:   5138    5138 
   Size:      10076
 
BtgI C'CryG_G

Cuts at:    107     123     375     435     495     615     698    1080    1130 
   Size:         16     252      60      60     120      83     382      50

Cuts at:   1130    1773    1884    2619    3405    4032    4143    4235    4261 
   Size:        643     111     735     786     627     111      92      26

Cuts at:   4261    4635    4766    5195    5402    6130    6216    6410    6430 
   Size:        374     131     429     207     728      86     194      20

Cuts at:   6430    6944     107 
   Size:        514    3239

  Fragments arranged by size: 

                3239     786     735     728     643     627     514     429
                 382     374     252     207     194     131     120     111
                 111      92      86      83      60      60      50      26
                  20      16
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1779    2375    2780    5868    8026    8207    8370    1779 
   Size:        596     405    3088    2158     181     163    3485

  Fragments arranged by size: 

                3485    3088    2158     596     405     181     163
 
BtsI GCAGTG_nn'

Cuts at:   2376    2865    2979    3100    3292    4003    4408    4902    4981 
   Size:        489     114     121     192     711     405     494      79

Cuts at:   4981    5041    5149    6497    6503    6515    6929    6964    7319 
   Size:         60     108    1348       6      12     414      35     355

Cuts at:   7319    2376 
   Size:       5133

  Fragments arranged by size: 

                5133    1348     711     494     489     414     405     355
                 192     121     114     108      79      60      35      12
                   6
 
ClaI AT'CG_AT

Cuts at:   1052    1052 
   Size:      10076
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:   5442    5477    6968    7003    5442 
   Size:         35    1491      35    8515

  Fragments arranged by size: 

                8515    1491      35      35
 
DraI TTT'AAA

Cuts at:   7075    7075 
   Size:      10076
 
DraIII CAC_nnn'GTG

Cuts at:    688    1730    2566    5282     688 
   Size:       1042     836    2716    5482

  Fragments arranged by size: 

                5482    2716    1042     836
 
DrdI GACnn_nn'nnGTC

Cuts at:    839    1171    3846    6394    7782    8640     839 
   Size:        332    2675    2548    1388     858    2275

  Fragments arranged by size: 

                2675    2548    2275    1388     858     332
 
EaeI y'GGCC_r

Cuts at:    126     372     996    1224    2478    4127    4491    4551    4758 
   Size:        246     624     228    1254    1649     364      60     207

Cuts at:   4758    5228    5258    6041    6947    7067    7661    7835    8226 
   Size:        470      30     783     906     120     594     174     391

Cuts at:   8226    8253    9598     126 
   Size:         27    1345     604

  Fragments arranged by size: 

                1649    1345    1254     906     783     624     604     594
                 470     391     364     246     228     207     174     120
                  60      30      27
 
EagI C'GGCC_G

Cuts at:   1224    4127    4551    7661    1224 
   Size:       2903     424    3110    3639

  Fragments arranged by size: 

                3639    3110    2903     424
 
EarI CTCTTCn'nnn_

Cuts at:   4420    5528    5870    6286    8099    8309    4420 
   Size:       1108     342     416    1813     210    6187

  Fragments arranged by size: 

                6187    1813    1108     416     342     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   3803    4732    8284    8594    8740    9886    3803 
   Size:        929    3552     310     146    1146    3993

  Fragments arranged by size: 

                3993    3552    1146     929     310     146
 
EcoICRI GAG'CTC

Cuts at:   5880    7088    5880 
   Size:       1208    8868
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    361    1563    1587    2298    3280    4879    5000    5029    5536 
   Size:       1202      24     711     982    1599     121      29     507

Cuts at:   5536    5552    6227    6587    6632    7901    8333    9080    9348 
   Size:         16     675     360      45    1269     432     747     268

Cuts at:   9348     361 
   Size:       1089

  Fragments arranged by size: 

                1599    1269    1202    1089     982     747     711     675
                 507     432     360     268     121      45      29      24
                  16
 
EcoNI CCTnn'n_nnAGG

Cuts at:   7009    7009 
   Size:      10076
 
EcoO109I rG'GnC_Cy

Cuts at:    119     345     361     448     517    1420    1421    2059    2060 
   Size:        226      16      87      69     903       1     638       1

Cuts at:   2060    2877    2918    3164    3398    3625    3626    3780    3826 
   Size:        817      41     246     234     227       1     154      46

Cuts at:   3826    3904    4069    6194    6226    6248    6302    6334    6335 
   Size:         78     165    2125      32      22      54      32       1

Cuts at:   6335    6376    6446    6481    6482    6652    6682    6704    7030 
   Size:         41      70      35       1     170      30      22     326

Cuts at:   7030    7031    7215     119 
   Size:          1     184    2980

  Fragments arranged by size: 

                2980    2125     903     817     638     326     246     234
                 227     226     184     170     165     154      87      78
                  70      69      54      46      41      41      35      32
                  32      30      22      22      16       1       1       1
                   1       1       1
 
EcoRI G'AATT_C

Cuts at:   7080    7080 
   Size:      10076
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   6825    8095    6825 
   Size:       1270    8806
 
FspI TGC'GCA

Cuts at:   4726    7857    4726 
   Size:       3131    6945
 
HaeII r_GCGC'y

Cuts at:    187    1304    1667    3044    4231    4308    4399    4413    4694 
   Size:       1117     363    1377    1187      77      91      14     281

Cuts at:   4694    4718    4928    4965    5060    6558    6679    7510    7758 
   Size:         24     210      37      95    1498     121     831     248

Cuts at:   7758    8780    9759     187 
   Size:       1022     979     504

  Fragments arranged by size: 

                1498    1377    1187    1117    1022     979     831     504
                 363     281     248     210     121      95      91      77
                  37      24      14
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    518     550    1026    1058    1276    1308    1399    1431    1738 
   Size:         32     476      32     218      32      91      32     307

Cuts at:   1738    1770    5152    5184    5539    5571    6563    6595    7918 
   Size:         32    3382      32     355      32     992      32    1323

Cuts at:   7918    7948    7950    7980     518 
   Size:         30       2      30    2614

  Fragments arranged by size: 

                3382    2614    1323     992     476     355     307     218
                  91      32      32      32      32      32      32      32
                  32      30      30       2
 
HincII GTy'rAC

Cuts at:    873    1404    1545    7115     873 
   Size:        531     141    5570    3834

  Fragments arranged by size: 

                5570    3834     531     141
 
HindIII A'AGCT_T

Cuts at:   3216    3216 
   Size:      10076
 
KasI G'GCGC_C

Cuts at:   1300    1663    4227    4409    6554    6675    7506    7754    1300 
   Size:        363    2564     182    2145     121     831     248    3622

  Fragments arranged by size: 

                3622    2564    2145     831     363     248     182     121
            
 
KpnI G_GTAC'C

Cuts at:    977    7096     977 
   Size:       6119    3957
 
MluI A'CGCG_T

Cuts at:   9509    9509 
   Size:      10076
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   5597    6062    8747    8931    5597 
   Size:        465    2685     184    6742

  Fragments arranged by size: 

                6742    2685     465     184
 
MscI TGG'CCA

Cuts at:    998    2480    5230    5260    6949    7837     998 
   Size:       1482    2750      30    1689     888    3237

  Fragments arranged by size: 

                3237    2750    1689    1482     888      30
 
MslI CAynn'nnrTG

Cuts at:    426    1069    1160    1193    1948    2201    3652    4487    5389 
   Size:        643      91      33     755     253    1451     835     902

Cuts at:   5389    5897    6508    8065    8192     426 
   Size:        508     611    1557     127    2310

  Fragments arranged by size: 

                2310    1557    1451     902     835     755     643     611
                 508     253     127      91      33
 
MspA1I CmG'CkG

Cuts at:    279     339     506     569     653     667     700     945    1004 
   Size:         60     167      63      84      14      33     245      59

Cuts at:   1004    1583    1607    1623    1703    1928    2147    2258    2291 
   Size:        579      24      16      80     225     219     111      33

Cuts at:   2291    2462    2484    2609    2948    3062    3267    3615    3677 
   Size:        171      22     125     339     114     205     348      62

Cuts at:   3677    4034    4121    4187    4237    4263    4430    4556    4637 
   Size:        357      87      66      50      26     167     126      81

Cuts at:   4637    4707    4930    4954    5007    5034    5743    6132    6432 
   Size:         70     223      24      53      27     709     389     300

Cuts at:   6432    6493    6690    7026    7187    7501    7861    8874    9119 
   Size:         61     197     336     161     314     360    1013     245

Cuts at:   9119     279 
   Size:       1236

  Fragments arranged by size: 

                1236    1013     709     579     389     360     357     348
                 339     336     314     300     245     245     225     223
                 219     205     197     171     167     167     161     126
                 125     114     111      87      84      81      80      70
                  66      63      62      61      60      59      53      50
                  33      33      27      26      24      24      22      16
                  14
 
NaeI GCC'GGC

Cuts at:   2748    4622    5185    8257    2748 
   Size:       1874     563    3072    4567

  Fragments arranged by size: 

                4567    3072    1874     563
 
NarI GG'CG_CC

Cuts at:   1301    1664    4228    4410    6555    6676    7507    7755    1301 
   Size:        363    2564     182    2145     121     831     248    3622

  Fragments arranged by size: 

                3622    2564    2145     831     363     248     182     121
            
 
NcoI C'CATG_G

Cuts at:    107     615    1080    1130    1773    1884    2619    4766    5402 
   Size:        508     465      50     643     111     735    2147     636

Cuts at:   5402    6944     107 
   Size:       1542    3239

  Fragments arranged by size: 

                3239    2147    1542     735     643     636     508     465
                 111      50
 
NgoMIV G'CCGG_C

Cuts at:   2746    4620    5183    8255    2746 
   Size:       1874     563    3072    4567

  Fragments arranged by size: 

                4567    3072    1874     563
 
NheI G'CTAG_C

Cuts at:    204    5560     204 
   Size:       5356    4720
 
NotI GC'GGCC_GC

Cuts at:   4127    4127 
   Size:      10076
 
NsiI A_TGCA'T

Cuts at:   3520    3520 
   Size:      10076
 
NspI r_CATG'y

Cuts at:    860    1271    2354    3429    6009    7129    7629    8536     860 
   Size:        411    1083    1075    2580    1120     500     907    2400

  Fragments arranged by size: 

                2580    2400    1120    1083    1075     907     500     411
            
 
PasI CC'CwG_GG

Cuts at:   2505    4057    5760    6700    2505 
   Size:       1552    1703     940    5881

  Fragments arranged by size: 

                5881    1703    1552     940
 
PciI A'CATG_T

Cuts at:   3425    8532    3425 
   Size:       5107    4969
 
PflMI CCAn_nnn'nTGG

Cuts at:   1281    2304    3105    4767    5267    6222    6757    1281 
   Size:       1023     801    1662     500     955     535    4600

  Fragments arranged by size: 

                4600    1662    1023     955     801     535     500
 
PfoI T'CCnGG_A

Cuts at:    828     828 
   Size:      10076
 
PmeI GTTT'AAAC

Cuts at:   7075    7075 
   Size:      10076
 
PmlI CAC'GTG

Cuts at:   1442    1616    2820    1442 
   Size:        174    1204    8698

  Fragments arranged by size: 

                8698    1204     174
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2677    2709    9215    9247    2677 
   Size:         32    6506      32    3506

  Fragments arranged by size: 

                6506    3506      32      32
 
PpuMI rG'GwC_Cy

Cuts at:    345    2877    2918    3398    3780    3826    3904    4069    6682 
   Size:       2532      41     480     382      46      78     165    2613

Cuts at:   6682    6704     345 
   Size:         22    3717

  Fragments arranged by size: 

                3717    2613    2532     480     382     165      78      46
                  41      22
 
PshAI GACnn'nnGTC

Cuts at:    350     824    1399    1523    6709     350 
   Size:        474     575     124    5186    3717

  Fragments arranged by size: 

                5186    3717     575     474     124
 
PspOMI G'GGCC_C

Cuts at:    119     517    1420    2059    3087    3625    4811    6193    6334 
   Size:        398     903     639    1028     538    1186    1382     141

Cuts at:   6334    6375    6445    6481    6623    7030     119 
   Size:         41      70      36     142     407    3165

  Fragments arranged by size: 

                3165    1382    1186    1028     903     639     538     407
                 398     142     141      70      41      36
 
PstI C_TGCA'G

Cuts at:    225     745     950    1042    1657    2221    2296    2893    3040 
   Size:        520     205      92     615     564      75     597     147

Cuts at:   3040    3229    3779    3998    4403    4447    4706    4886    6510 
   Size:        189     550     219     405      44     259     180    1624

Cuts at:   6510    7123    7808     225 
   Size:        613     685    2493

  Fragments arranged by size: 

                2493    1624     685     615     613     597     564     550
                 520     405     259     219     205     189     180     147
                  92      75      44
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:      10076
 
PvuII CAG'CTG

Cuts at:    279     339     569     667     945    1004    1583    1623    2484 
   Size:         60     230      98     278      59     579      40     861

Cuts at:   2484    2609    2948    3267    4121    5743    6493    6690    7026 
   Size:        125     339     319     854    1622     750     197     336

Cuts at:   7026    7501    7861     279 
   Size:        475     360    2494

  Fragments arranged by size: 

                2494    1622     861     854     750     579     475     360
                 339     336     319     278     230     197     125      98
                  60      59      40
 
RsrII CG'GwC_CG

Cuts at:   8271    8271 
   Size:      10076
 
SacI G_AGCT'C

Cuts at:   5882    7090    5882 
   Size:       1208    8868
 
SacII CC_GC'GG

Cuts at:    701    4035    4238    4264    4638    6133    6433     701 
   Size:       3334     203      26     374    1495     300    4344

  Fragments arranged by size: 

                4344    3334    1495     374     300     203      26
 
SalI G'TCGA_C

Cuts at:   7113    7113 
   Size:      10076
 
SanDI GG'GwC_CC

Cuts at:   4069    6682    6704    4069 
   Size:       2613      22    7441

  Fragments arranged by size: 

                7441    2613      22
 
SapI GCTCTTCn'nnn_

Cuts at:   5870    6286    8099    8309    5870 
   Size:        416    1813     210    7637

  Fragments arranged by size: 

                7637    1813     416     210
 
SbfI CC_TGCA'GG

Cuts at:    745    1657    3229    3779    7123     745 
   Size:        912    1572     550    3344    3698

  Fragments arranged by size: 

                3698    3344    1572     912     550
 
ScaI AGT'ACT

Cuts at:   4528    9593    4528 
   Size:       5065    5011
 
SexAI A'CCwGG_T

Cuts at:   1904    3659    1904 
   Size:       1755    8321
 
SfcI C'TryA_G

Cuts at:     33     221     741     946    1038    1653    2217    2292    2889 
   Size:        188     520     205      92     615     564      75     597

Cuts at:   2889    3036    3225    3775    3994    4399    4443    4702    4882 
   Size:        147     189     550     219     405      44     259     180

Cuts at:   4882    5795    6506    7119    7804    8797    8988      33 
   Size:        913     711     613     685     993     191    1121

  Fragments arranged by size: 

                1121     993     913     711     685     615     613     597
                 564     550     520     405     259     219     205     191
                 189     188     180     147      92      75      44
 
SfiI GGCCn_nnn'nGGCC

Cuts at:   4630    6333    4630 
   Size:       1703    8373
 
SfoI GGC'GCC

Cuts at:   1302    1665    4229    4411    6556    6677    7508    7756    1302 
   Size:        363    2564     182    2145     121     831     248    3622

  Fragments arranged by size: 

                3622    2564    2145     831     363     248     182     121
            
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:      10076
 
SgrAI Cr'CCGG_yG

Cuts at:   4486    4486 
   Size:      10076
 
SmaI CCC'GGG

Cuts at:    289    2801    3766    3902    4091    4810    6309    6340    6374 
   Size:       2512     965     136     189     719    1499      31      34

Cuts at:   6374    7098     289 
   Size:        724    3267

  Fragments arranged by size: 

                3267    2512    1499     965     724     719     189     136
                  34      31
 
SmlI C'TyrA_G

Cuts at:   3073    6475    7231    8146    8638    8900    3073 
   Size:       3402     756     915     492     262    4249

  Fragments arranged by size: 

                4249    3402     915     756     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:      10076
 
SphI G_CATG'C

Cuts at:   7129    7629    7129 
   Size:        500    9576
 
SrfI GCCC'GGGC

Cuts at:   2801    6374    2801 
   Size:       3573    6503
 
StyI C'CwwG_G

Cuts at:    107     615    1080    1130    1773    1884    2619    4766    5231 
   Size:        508     465      50     643     111     735    2147     465

Cuts at:   5231    5402    6944    7210    7564     107 
   Size:        171    1542     266     354    2619

  Fragments arranged by size: 

                2619    2147    1542     735     643     508     465     465
                 354     266     171     111      50
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    289     309     313     589     982    1411    1580    3655    5077 
   Size:         20       4     276     393     429     169    2075    1422

Cuts at:   5077    6384    7659    7792    8251    8460     289 
   Size:       1307    1275     133     459     209    1905

  Fragments arranged by size: 

                2075    1905    1422    1307    1275     459     429     393
                 276     209     169     133      20       4
 
TatI w'GTAC_w

Cuts at:   4526    6576    9591    4526 
   Size:       2050    3015    5011

  Fragments arranged by size: 

                5011    3015    2050
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1230    1262    1932    1964    2168    2200    2809    2841    3253 
   Size:         32     670      32     204      32     609      32     412

Cuts at:   3253    3285    3390    3422    3982    4014    4135    4167    4249 
   Size:         32     105      32     560      32     121      32      82

Cuts at:   4249    4281    5266    5298    9593    9625    1230 
   Size:         32     985      32    4295      32    1681

  Fragments arranged by size: 

                4295    1681     985     670     609     560     412     204
                 121     105      82      32      32      32      32      32
                  32      32      32      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1567    2035    7873    1567 
   Size:        468    5838    3770

  Fragments arranged by size: 

                5838    3770     468
 
XbaI T'CTAG_A

Cuts at:   7107    7107 
   Size:      10076
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    664    1366    1620    4337    5268    5679    6078    6942    7571 
   Size:        702     254    2717     931     411     399     864     629

Cuts at:   7571     664 
   Size:       3169

  Fragments arranged by size: 

                3169    2717     931     864     702     629     411     399
                 254
 
XhoI C'TCGA_G

Cuts at:   3073    3073 
   Size:      10076
 
XmaI C'CCGG_G

Cuts at:    287    2799    3764    3900    4089    4808    6307    6338    6372 
   Size:       2512     965     136     189     719    1499      31      34

Cuts at:   6372    7096     287 
   Size:        724    3267

  Fragments arranged by size: 

                3267    2512    1499     965     724     719     189     136
                  34      31
 
XmnI GAAnn'nnTTC

Cuts at:   2941    2941 
   Size:      10076
 
ZraI GAC'GTC

Cuts at:   2407    2407 
   Size:      10076
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI   AflIII
     AgeI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AseI    AsiSI     AvaI     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI
    BstBI    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI    HaeII
    Hin4I   HincII  HindIII     KasI     KpnI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI     NotI
     NsiI     NspI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI   PspOMI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI     SpeI
     SphI     SrfI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

    AflII     AhdI     AscI    AvrII    BsiWI    BsrGI   BstEII  BstZ17I
    EcoRV     FseI    FspAI     HpaI     MfeI     NdeI     NruI     PacI
     PsiI    PspXI     PsrI    SnaBI     SspI     StuI     SwaI
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