Restriction map for pF1KB6755
 (Circular) (Six-base) MAPSORT of: pF1KB6755.seq  Check: 1556  from: 1  to: 3428



pF1KB6755  3428 bp

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:48  ..

AccI GT'mk_AC

Cuts at:    466     466 
   Size:       3428
 
Acc65I G'GTAC_C

Cuts at:    444     444 
   Size:       3428
 
AclI AA'CG_TT

Cuts at:   3268    3268 
   Size:       3428
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    247    1253    1685    2432    2700     247 
   Size:       1006     432     747     268     975

  Fragments arranged by size: 

                1006     975     747     432     268
 
AflIII A'CryG_T

Cuts at:    780    1884    2861     780 
   Size:       1104     977    1347

  Fragments arranged by size: 

                1347    1104     977
 
AgeI A'CCGG_T

Cuts at:   1816    1816 
   Size:       3428
 
AlwNI CAG_nnn'CTG

Cuts at:    733    2300     733 
   Size:       1567    1861
 
ApaLI G'TGCA_C

Cuts at:   2198    2198 
   Size:       3428
 
ApoI r'AATT_y

Cuts at:    432    2781    3037     432 
   Size:       2349     256     823

  Fragments arranged by size: 

                2349     823     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3428
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3428
 
AvaI C'yCGr_G

Cuts at:    162     214     448     162 
   Size:         52     234    3142

  Fragments arranged by size: 

                3142     234      52
 
BamHI G'GATC_C

Cuts at:    453     453 
   Size:       3428
 
BanI G'GyrC_C

Cuts at:    444     858    1106    1141     444 
   Size:        414     248      35    2731

  Fragments arranged by size: 

                2731     414     248      35
 
BanII G_rGCy'C

Cuts at:    442    1472     442 
   Size:       1030    2398
 
BbeI G_GCGC'C

Cuts at:    862    1110     862 
   Size:        248    3180
 
BbvCI CC'TCA_GC

Cuts at:    233     233 
   Size:       3428
 
BciVI GTATCCnnnnn_n'

Cuts at:   1326    2087    3428    1326 
   Size:        761    1341    1326

  Fragments arranged by size: 

                1341    1326     761
 
BclI T'GATC_A

Cuts at:    948     948 
   Size:       3428
 
BglII A'GATC_T

Cuts at:    943    2980     943 
   Size:       2037    1391
 
BlpI GC'TnA_GC

Cuts at:    540     540 
   Size:       3428
 
Bme1580I G_kGCm'C

Cuts at:   1053    1146    2202    1053 
   Size:         93    1056    2279

  Fragments arranged by size: 

                2279    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    291     395     640     721    1056    3200     291 
   Size:        104     245      81     335    2144     519

  Fragments arranged by size: 

                2144     519     335     245     104      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1795    1795 
   Size:       3428
 
Bpu10I CC'TnA_GC

Cuts at:    233     233 
   Size:       3428
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    604    1483    1975    2273     604 
   Size:        879     492     298    1759

  Fragments arranged by size: 

                1759     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    217    3132     217 
   Size:       2915     513
 
BsaAI yAC'GTr

Cuts at:    781     781 
   Size:       3428
 
BsaBI GATnn'nnATC

Cuts at:      6     942    2979       6 
   Size:        936    2037     455

  Fragments arranged by size: 

                2037     936     455
 
BsaHI Gr'CG_yC

Cuts at:    120     859    1107    1809    3305     120 
   Size:        739     248     702    1496     243

  Fragments arranged by size: 

                1496     739     702     248     243
 
BsaWI w'CCGG_w

Cuts at:    362     617    1138    1816    2090    2237    2648     362 
   Size:        255     521     678     274     147     411    1142

  Fragments arranged by size: 

                1142     678     521     411     274     255     147
 
BseYI C'CCAG_C

Cuts at:    853    1611    2188    2684     853 
   Size:        758     577     496    1597

  Fragments arranged by size: 

                1597     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104     970    1016    2224     104 
   Size:        866      46    1208    1308

  Fragments arranged by size: 

                1308    1208     866      46
 
BsiHKAI G_wGCw'C

Cuts at:    442    1220    1410    2202     442 
   Size:        778     190     792    1668

  Fragments arranged by size: 

                1668     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    442    1053    1146    1220    1410    1472    2202     442 
   Size:        611      93      74     190      62     730    1668

  Fragments arranged by size: 

                1668     730     611     190      93      74      62
 
BspEI T'CCGG_A

Cuts at:    362    2648     362 
   Size:       2286    1142
 
BspHI T'CATG_A

Cuts at:   2604    2604 
   Size:       3428
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    147     478     692     994    1375     147 
   Size:        331     214     302     381    2200

  Fragments arranged by size: 

                2200     381     331     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1720    1774    3258    3423      50 
   Size:       1670      54    1484     165      55

  Fragments arranged by size: 

                1670    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1340    1340 
   Size:       3428
 
BsrFI r'CCGG_y

Cuts at:   1426    1607    1816    1426 
   Size:        181     209    3038

  Fragments arranged by size: 

                3038     209     181
 
BssSI C'ACGA_G

Cuts at:   1699    2057    1699 
   Size:        358    3070
 
BstAPI GCAn_nnn'nTGC

Cuts at:    805     805 
   Size:       3428
 
BstBI TT'CG_AA

Cuts at:   1789    1789 
   Size:       3428
 
BstYI r'GATC_y

Cuts at:      1     453     920     943    1278    1524    2536    2622    2634 
   Size:        452     467      23     335     246    1012      86      12

Cuts at:   2634    2980       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     467     452     449     346     335     246      86
                  23      12
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1378    1559    1722    1378 
   Size:        181     163    3084

  Fragments arranged by size: 

                3084     181     163
 
BtsI GCAGTG_nn'

Cuts at:    671     671 
   Size:       3428
 
DraI TTT'AAA

Cuts at:    384     427     384 
   Size:         43    3385
 
DrdI GACnn_nn'nnGTC

Cuts at:   1134    1992    1134 
   Size:        858    2570
 
EaeI y'GGCC_r

Cuts at:   1013    1187    1578    1605    2950    1013 
   Size:        174     391      27    1345    1491

  Fragments arranged by size: 

                1491    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1013    1013 
   Size:       3428
 
EarI CTCTTCn'nnn_

Cuts at:   1451    1661    1451 
   Size:        210    3218
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1636    1946    2092    3238    1636 
   Size:        310     146    1146    1826

  Fragments arranged by size: 

                1826    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    440     440 
   Size:       3428
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    247    1253    1685    2432    2700     247 
   Size:       1006     432     747     268     975

  Fragments arranged by size: 

                1006     975     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    159     567     159 
   Size:        408    3020
 
EcoRI G'AATT_C

Cuts at:    432     432 
   Size:       3428
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1447    1447 
   Size:       3428
 
FspI TGC'GCA

Cuts at:   1209    1209 
   Size:       3428
 
HaeII r_GCGC'y

Cuts at:    233     862    1110    2132    3111     233 
   Size:        629     248    1022     979     550

  Fragments arranged by size: 

                1022     979     629     550     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1270    1300    1302    1332    1270 
   Size:         30       2      30    3366

  Fragments arranged by size: 

                3366      30      30       2
 
HincII GTy'rAC

Cuts at:    467     467 
   Size:       3428
 
KasI G'GCGC_C

Cuts at:    858    1106     858 
   Size:        248    3180
 
KpnI G_GTAC'C

Cuts at:    448     448 
   Size:       3428
 
MluI A'CGCG_T

Cuts at:   2861    2861 
   Size:       3428
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2099    2283    2099 
   Size:        184    3244
 
MscI TGG'CCA

Cuts at:   1189    1189 
   Size:       3428
 
MslI CAynn'nnrTG

Cuts at:   1417    1544    1417 
   Size:        127    3301
 
MspA1I CmG'CkG

Cuts at:    539     853    1213    2226    2471     539 
   Size:        314     360    1013     245    1496

  Fragments arranged by size: 

                1496    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   1609    1609 
   Size:       3428
 
NarI GG'CG_CC

Cuts at:    859    1107     859 
   Size:        248    3180
 
NgoMIV G'CCGG_C

Cuts at:   1607    1607 
   Size:       3428
 
NspI r_CATG'y

Cuts at:    481     981    1888     481 
   Size:        500     907    2021

  Fragments arranged by size: 

                2021     907     500
 
PciI A'CATG_T

Cuts at:   1884    1884 
   Size:       3428
 
PmeI GTTT'AAAC

Cuts at:    427     427 
   Size:       3428
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2567    2599    2567 
   Size:         32    3396
 
PpuMI rG'GwC_Cy

Cuts at:    159     159 
   Size:       3428
 
PstI C_TGCA'G

Cuts at:    243     475    1160     243 
   Size:        232     685    2511

  Fragments arranged by size: 

                2511     685     232
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3428
 
PvuII CAG'CTG

Cuts at:    853    1213     853 
   Size:        360    3068
 
RsrII CG'GwC_CG

Cuts at:   1623    1623 
   Size:       3428
 
SacI G_AGCT'C

Cuts at:    442     442 
   Size:       3428
 
SalI G'TCGA_C

Cuts at:    465     465 
   Size:       3428
 
SapI GCTCTTCn'nnn_

Cuts at:   1451    1661    1451 
   Size:        210    3218
 
SbfI CC_TGCA'GG

Cuts at:    475     475 
   Size:       3428
 
ScaI AGT'ACT

Cuts at:   2945    2945 
   Size:       3428
 
SfcI C'TryA_G

Cuts at:     33     224     239     471    1156    2149    2340      33 
   Size:        191      15     232     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     232     191     191      15
 
SfoI GGC'GCC

Cuts at:    860    1108     860 
   Size:        248    3180
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3428
 
SmaI CCC'GGG

Cuts at:    450     450 
   Size:       3428
 
SmlI C'TyrA_G

Cuts at:    583    1498    1990    2252     583 
   Size:        915     492     262    1759

  Fragments arranged by size: 

                1759     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3428
 
SphI G_CATG'C

Cuts at:    481     981     481 
   Size:        500    2928
 
StyI C'CwwG_G

Cuts at:    207     562     916     207 
   Size:        355     354    2719

  Fragments arranged by size: 

                2719     355     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1011    1144    1603    1812    1011 
   Size:        133     459     209    2627

  Fragments arranged by size: 

                2627     459     209     133
 
TatI w'GTAC_w

Cuts at:   2943    2943 
   Size:       3428
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2945    2977    2945 
   Size:         32    3396
 
Tth111I GACn'n_nGTC

Cuts at:   1225    1225 
   Size:       3428
 
XbaI T'CTAG_A

Cuts at:    459     459 
   Size:       3428
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    923     923 
   Size:       3428
 
XmaI C'CCGG_G

Cuts at:    448     448 
   Size:       3428
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
    BbvCI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI     BplI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseYI
    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BssSI   BstAPI    BstBI    BstYI    BtgZI     BtsI     DraI
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I
    EcoRI     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV
     NspI     PciI     PmeI     PpiI    PpuMI     PstI     PvuI    PvuII
    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI     SfoI
     SgfI     SmaI     SmlI     SpeI     SphI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI     BbsI     BcgI    BfrBI     BglI
    BmgBI     BmtI     BpmI    BsaXI    BseRI     BsgI    BsiWI     BsmI
    BsmBI    BsrGI   BssHII   BstEII    BstXI  BstZ17I   Bsu36I     BtgI
     ClaI    CspCI   DraIII    EcoNI    EcoRV     FseI    FspAI  HindIII
     HpaI     MfeI     NcoI     NdeI     NheI     NotI     NruI     NsiI
     PacI     PasI    PflMI     PfoI     PmlI    PshAI     PsiI   PspOMI
    PspXI     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI
     SrfI     SspI     StuI     SwaI     XhoI     XmnI     ZraI
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