Restriction map for pF1KE0531
 (Circular) (Six-base) MAPSORT of: pF1KE0531.seq  Check: 4000  from: 1  to: 4346



pF1KE0531  4346 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:15  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    493     493 
   Size:       4346
 
AatII G_ACGT'C

Cuts at:   1220    1220 
   Size:       4346
 
AccI GT'mk_AC

Cuts at:    557    1220    1384     557 
   Size:        663     164    3519

  Fragments arranged by size: 

                3519     663     164
 
Acc65I G'GTAC_C

Cuts at:    611    1301    1362     611 
   Size:        690      61    3595

  Fragments arranged by size: 

                3595     690      61
 
AclI AA'CG_TT

Cuts at:   4186    4186 
   Size:       4346
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    136     469    1113    1268    2171    2603    3350    3618     136 
   Size:        333     644     155     903     432     747     268     864

  Fragments arranged by size: 

                 903     864     747     644     432     333     268     155
            
 
AfeI AGC'GCT

Cuts at:    397     397 
   Size:       4346
 
AflIII A'CryG_T

Cuts at:    138    1698    2802    3779     138 
   Size:       1560    1104     977     705

  Fragments arranged by size: 

                1560    1104     977     705
 
AgeI A'CCGG_T

Cuts at:   2734    2734 
   Size:       4346
 
AlwNI CAG_nnn'CTG

Cuts at:    153    1651    3218     153 
   Size:       1498    1567    1281

  Fragments arranged by size: 

                1567    1498    1281
 
ApaI G_GGCC'C

Cuts at:    606     912     606 
   Size:        306    4040
 
ApaLI G'TGCA_C

Cuts at:   3116    3116 
   Size:       4346
 
ApoI r'AATT_y

Cuts at:   1350    3699    3955    1350 
   Size:       2349     256    1741

  Fragments arranged by size: 

                2349    1741     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4346
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4346
 
AvaI C'yCGr_G

Cuts at:    592     905    1366     592 
   Size:        313     461    3572

  Fragments arranged by size: 

                3572     461     313
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:    951     984     951 
   Size:         33    4313
 
BamHI G'GATC_C

Cuts at:    387    1029    1371     387 
   Size:        642     342    3362

  Fragments arranged by size: 

                3362     642     342
 
BanI G'GyrC_C

Cuts at:    502     611    1301    1362    1776    2024    2059     502 
   Size:        109     690      61     414     248      35    2789

  Fragments arranged by size: 

                2789     690     414     248     109      61      35
 
BanII G_rGCy'C

Cuts at:    173     606     912    1360    2390     173 
   Size:        433     306     448    1030    2129

  Fragments arranged by size: 

                2129    1030     448     433     306
 
BbeI G_GCGC'C

Cuts at:   1780    2028    1780 
   Size:        248    4098
 
BbsI GAAGACnn'nnnn_

Cuts at:    124    1116     124 
   Size:        992    3354
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:     90     124      90 
   Size:         34    4312
 
BciVI GTATCCnnnnn_n'

Cuts at:   1319    2244    3005    4346    1319 
   Size:        925     761    1341    1319

  Fragments arranged by size: 

                1341    1319     925     761
 
BclI T'GATC_A

Cuts at:   1013    1271    1866    1013 
   Size:        258     595    3493

  Fragments arranged by size: 

                3493     595     258
 
BglI GCCn_nnn'nGGC

Cuts at:    916     916 
   Size:       4346
 
BglII A'GATC_T

Cuts at:   1861    3898    1861 
   Size:       2037    2309
 
BlpI GC'TnA_GC

Cuts at:   1458    1458 
   Size:       4346
 
Bme1580I G_kGCm'C

Cuts at:    606     848     912    1971    2064    3120     606 
   Size:        242      64    1059      93    1056    1832

  Fragments arranged by size: 

                1832    1059    1056     242      93      64
 
BmrI ACTGGGnnnn_n'

Cuts at:   1558    1639    1974    4118    1558 
   Size:         81     335    2144    1786

  Fragments arranged by size: 

                2144    1786     335      81
 
BmtI G_CTAG'C

Cuts at:   1115    1115 
   Size:       4346
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2713    2713 
   Size:       4346
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1345    1345 
   Size:       4346
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    550    1522    2401    2893    3191     550 
   Size:        972     879     492     298    1705

  Fragments arranged by size: 

                1705     972     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    892    4050     892 
   Size:       3158    1188
 
BsaAI yAC'GTr

Cuts at:   1699    1699 
   Size:       4346
 
BsaBI GATnn'nnATC

Cuts at:      6    1018    1860    3897       6 
   Size:       1012     842    2037     455

  Fragments arranged by size: 

                2037    1012     842     455
 
BsaHI Gr'CG_yC

Cuts at:   1217    1777    2025    2727    4223    1217 
   Size:        560     248     702    1496    1340

  Fragments arranged by size: 

                1496    1340     702     560     248
 
BsaWI w'CCGG_w

Cuts at:    287    1535    2056    2734    3008    3155    3566     287 
   Size:       1248     521     678     274     147     411    1067

  Fragments arranged by size: 

                1248    1067     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    421     451    1150    1180     421 
   Size:         30     699      30    3587

  Fragments arranged by size: 

                3587     699      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    610    1155     610 
   Size:        545    3801
 
BseYI C'CCAG_C

Cuts at:    132    1334    1771    2529    3106    3602     132 
   Size:       1202     437     758     577     496     876

  Fragments arranged by size: 

                1202     876     758     577     496     437
 
BsiEI CG_ry'CG

Cuts at:    104    1888    1934    3142     104 
   Size:       1784      46    1208    1308

  Fragments arranged by size: 

                1784    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    128     785     812    1360    2138    2328    3120     128 
   Size:        657      27     548     778     190     792    1354

  Fragments arranged by size: 

                1354     792     778     657     548     190      27
 
BsmI GAATG_Cn'

Cuts at:    410     642     410 
   Size:        232    4114
 
Bsp1286I G_dGCh'C

Cuts at:    128     173     606     785     812     848     912    1360    1971 
   Size:         45     433     179      27      36      64     448     611

Cuts at:   1971    2064    2138    2328    2390    3120     128 
   Size:         93      74     190      62     730    1354

  Fragments arranged by size: 

                1354     730     611     448     433     190     179      93
                  74      64      62      45      36      27
 
BspEI T'CCGG_A

Cuts at:   3566    3566 
   Size:       4346
 
BspHI T'CATG_A

Cuts at:   1010    3522    1010 
   Size:       2512    1834
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    493     971    1329    1396    1610    1912    2293     493 
   Size:        478     358      67     214     302     381    2546

  Fragments arranged by size: 

                2546     478     381     358     302     214      67
 
BsrBI CCG'CTC

Cuts at:     50     600    2638    2692    4176    4341      50 
   Size:        550    2038      54    1484     165      55

  Fragments arranged by size: 

                2038    1484     550     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2258    2258 
   Size:       4346
 
BsrFI r'CCGG_y

Cuts at:    835    2344    2525    2734     835 
   Size:       1509     181     209    2447

  Fragments arranged by size: 

                2447    1509     209     181
 
BsrGI T'GTAC_A

Cuts at:   1085    1085 
   Size:       4346
 
BssSI C'ACGA_G

Cuts at:   2617    2975    2617 
   Size:        358    3988
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1723    1723 
   Size:       4346
 
BstBI TT'CG_AA

Cuts at:   2707    2707 
   Size:       4346
 
BstEII G'GTnAC_C

Cuts at:    493     493 
   Size:       4346
 
BstYI r'GATC_y

Cuts at:      1     387    1029    1371    1838    1861    2196    2442    3454 
   Size:        386     642     342     467      23     335     246    1012

Cuts at:   3454    3540    3552    3898       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1012     642     467     449     386     346     342     335
                 246      86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:   1035    1035 
   Size:       4346
 
BtgI C'CryG_G

Cuts at:    188     188 
   Size:       4346
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    377     639    2296    2477    2640     377 
   Size:        262    1657     181     163    2083

  Fragments arranged by size: 

                2083    1657     262     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1589    1589 
   Size:       4346
 
DraI TTT'AAA

Cuts at:   1345    1345 
   Size:       4346
 
DrdI GACnn_nn'nnGTC

Cuts at:    520    2052    2910     520 
   Size:       1532     858    1956

  Fragments arranged by size: 

                1956    1532     858
 
EaeI y'GGCC_r

Cuts at:    372     673     833    1173    1931    2105    2496    2523    3868 
   Size:        301     160     340     758     174     391      27    1345

Cuts at:   3868     372 
   Size:        850

  Fragments arranged by size: 

                1345     850     758     391     340     301     174     160
                  27
 
EagI C'GGCC_G

Cuts at:   1931    1931 
   Size:       4346
 
EarI CTCTTCn'nnn_

Cuts at:    274    2369    2579     274 
   Size:       2095     210    2041

  Fragments arranged by size: 

                2095    2041     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    739     966    2554    2864    3010    4156     739 
   Size:        227    1588     310     146    1146     929

  Fragments arranged by size: 

                1588    1146     929     310     227     146
 
EcoICRI GAG'CTC

Cuts at:   1358    1358 
   Size:       4346
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    136     469    1113    1268    1345    2171    2603    3350    3618 
   Size:        333     644     155      77     826     432     747     268

Cuts at:   3618     136 
   Size:        864

  Fragments arranged by size: 

                 864     826     747     644     432     333     268     155
                  77
 
EcoO109I rG'GnC_Cy

Cuts at:    240    1485     240 
   Size:       1245    3101
 
EcoRI G'AATT_C

Cuts at:   1350    1350 
   Size:       4346
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2365    2365 
   Size:       4346
 
FseI GG_CCGG'CC

Cuts at:    839     839 
   Size:       4346
 
FspI TGC'GCA

Cuts at:   2127    2127 
   Size:       4346
 
HaeII r_GCGC'y

Cuts at:    399    1118    1780    2028    3050    4029     399 
   Size:        719     662     248    1022     979     716

  Fragments arranged by size: 

                1022     979     719     716     662     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    342     372     374     404    1207    1239    2188    2218    2220 
   Size:         30       2      30     803      32     949      30       2

Cuts at:   2220    2250     342 
   Size:         30    2438

  Fragments arranged by size: 

                2438     949     803      32      30      30      30      30
                   2       2
 
HincII GTy'rAC

Cuts at:   1385    1385 
   Size:       4346
 
KasI G'GCGC_C

Cuts at:   1776    2024    1776 
   Size:        248    4098
 
KpnI G_GTAC'C

Cuts at:    615    1305    1366     615 
   Size:        690      61    3595

  Fragments arranged by size: 

                3595     690      61
 
MluI A'CGCG_T

Cuts at:   3779    3779 
   Size:       4346
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1127    3017    3201    1127 
   Size:       1890     184    2272

  Fragments arranged by size: 

                2272    1890     184
 
MscI TGG'CCA

Cuts at:    675    1175    2107     675 
   Size:        500     932    2914

  Fragments arranged by size: 

                2914     932     500
 
MslI CAynn'nnrTG

Cuts at:    277     487     851    1081    2335    2462     277 
   Size:        210     364     230    1254     127    2161

  Fragments arranged by size: 

                2161    1254     364     230     210     127
 
MspA1I CmG'CkG

Cuts at:    218     671    1133    1179    1457    1771    2131    3144    3389 
   Size:        453     462      46     278     314     360    1013     245

Cuts at:   3389     218 
   Size:       1175

  Fragments arranged by size: 

                1175    1013     462     453     360     314     278     245
                  46
 
NaeI GCC'GGC

Cuts at:    837    2527     837 
   Size:       1690    2656
 
NarI GG'CG_CC

Cuts at:   1777    2025    1777 
   Size:        248    4098
 
NcoI C'CATG_G

Cuts at:    188     188 
   Size:       4346
 
NgoMIV G'CCGG_C

Cuts at:    835    2525     835 
   Size:       1690    2656
 
NheI G'CTAG_C

Cuts at:   1111    1111 
   Size:       4346
 
NruI TCG'CGA

Cuts at:    362     362 
   Size:       4346
 
NspI r_CATG'y

Cuts at:    142     564     867    1092    1399    1899    2806     142 
   Size:        422     303     225     307     500     907    1682

  Fragments arranged by size: 

                1682     907     500     422     307     303     225
 
PasI CC'CwG_GG

Cuts at:    441     606     841     441 
   Size:        165     235    3946

  Fragments arranged by size: 

                3946     235     165
 
PciI A'CATG_T

Cuts at:    138    2802     138 
   Size:       2664    1682
 
PflMI CCAn_nnn'nTGG

Cuts at:    194     987     194 
   Size:        793    3553
 
PfoI T'CCnGG_A

Cuts at:    524     761     524 
   Size:        237    4109
 
PmeI GTTT'AAAC

Cuts at:   1345    1345 
   Size:       4346
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3485    3517    3485 
   Size:         32    4314
 
PpuMI rG'GwC_Cy

Cuts at:    240     240 
   Size:       4346
 
PspOMI G'GGCC_C

Cuts at:    602     908     602 
   Size:        306    4040
 
PstI C_TGCA'G

Cuts at:    179     670     827    1001    1138    1393    2078     179 
   Size:        491     157     174     137     255     685    2447

  Fragments arranged by size: 

                2447     685     491     255     174     157     137
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4346
 
PvuII CAG'CTG

Cuts at:    671    1133    1179    1771    2131     671 
   Size:        462      46     592     360    2886

  Fragments arranged by size: 

                2886     592     462     360      46
 
RsrII CG'GwC_CG

Cuts at:   2541    2541 
   Size:       4346
 
SacI G_AGCT'C

Cuts at:   1360    1360 
   Size:       4346
 
SalI G'TCGA_C

Cuts at:   1383    1383 
   Size:       4346
 
SapI GCTCTTCn'nnn_

Cuts at:    274    2369    2579     274 
   Size:       2095     210    2041

  Fragments arranged by size: 

                2095    2041     210
 
SbfI CC_TGCA'GG

Cuts at:   1393    1393 
   Size:       4346
 
ScaI AGT'ACT

Cuts at:   3863    3863 
   Size:       4346
 
SfcI C'TryA_G

Cuts at:     33     175     666     711     823     997    1134    1389    2074 
   Size:        142     491      45     112     174     137     255     685

Cuts at:   2074    3067    3258      33 
   Size:        993     191    1121

  Fragments arranged by size: 

                1121     993     685     491     255     191     174     142
                 137     112      45
 
SfiI GGCCn_nnn'nGGCC

Cuts at:    916     916 
   Size:       4346
 
SfoI GGC'GCC

Cuts at:   1778    2026    1778 
   Size:        248    4098
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4346
 
SmaI CCC'GGG

Cuts at:    907    1368     907 
   Size:        461    3885
 
SmlI C'TyrA_G

Cuts at:    565    1501    2416    2908    3170     565 
   Size:        936     915     492     262    1741

  Fragments arranged by size: 

                1741     936     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4346
 
SphI G_CATG'C

Cuts at:   1399    1899    1399 
   Size:        500    3846
 
StyI C'CwwG_G

Cuts at:    188     700    1170    1480    1834     188 
   Size:        512     470     310     354    2700

  Fragments arranged by size: 

                2700     512     470     354     310
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    201    1156    1929    2062    2521    2730     201 
   Size:        955     773     133     459     209    1817

  Fragments arranged by size: 

                1817     955     773     459     209     133
 
TatI w'GTAC_w

Cuts at:   1085    3861    1085 
   Size:       2776    1570
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    757     789    3863    3895     757 
   Size:         32    3074      32    1208

  Fragments arranged by size: 

                3074    1208      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   2143    2143 
   Size:       4346
 
XbaI T'CTAG_A

Cuts at:   1377    1377 
   Size:       4346
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    191     195    1168    1320    1841     191 
   Size:          4     973     152     521    2696

  Fragments arranged by size: 

                2696     973     521     152       4
 
XmaI C'CCGG_G

Cuts at:    905    1366     905 
   Size:        461    3885
 
XmnI GAAnn'nnTTC

Cuts at:    799     799 
   Size:       4346
 
ZraI GAC'GTC

Cuts at:   1218    1218 
   Size:       4346
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI   AflIII
     AgeI    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI
     BaeI    BamHI     BanI    BanII     BbeI     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I     BmrI     BmtI     BplI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI
   BstEII    BstYI   Bsu36I     BtgI    BtgZI     BtsI     DraI     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI
     FalI     FseI     FspI    HaeII    Hin4I   HincII     KasI     KpnI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NheI     NruI     NspI     PasI     PciI    PflMI     PfoI
     PmeI     PpiI    PpuMI   PspOMI     PstI     PvuI    PvuII    RsrII
     SacI     SalI     SapI     SbfI     ScaI     SfcI     SfiI     SfoI
     SgfI     SmaI     SmlI     SpeI     SphI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

    AflII     AhdI     AleI     AlfI     AloI     AscI    AvrII    BbvCI
    BfrBI    BmgBI   Bpu10I     BsgI    BsiWI    BsmBI   BssHII    BstXI
  BstZ17I     ClaI    CspCI   DraIII    EcoNI    EcoRV    FspAI  HindIII
     HpaI     MfeI     NdeI     NotI     NsiI     PacI     PmlI    PshAI
     PsiI    PspXI     PsrI    SacII    SanDI    SexAI    SgrAI    SnaBI
     SrfI     SspI     StuI     SwaI     XhoI
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