Restriction map for pF1KB7807
 (Linear) (Six-base) MAPSORT of: KIBB7807.seq  Check: 529  from: 1  to: 1575



KIBB7807.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:49  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     670    1575 
   Size:        670     905
 
Acc65I G'GTAC_C

Cuts at:      0     403    1575 
   Size:        403    1172
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     321    1121    1144    1575 
   Size:        321     800      23     431

  Fragments arranged by size: 

                 800     431     321      23
 
AlwNI CAG_nnn'CTG

Cuts at:      0     213     352    1189    1374    1439    1575 
   Size:        213     139     837     185      65     136

  Fragments arranged by size: 

                 837     213     185     139     136      65
 
AvaI C'yCGr_G

Cuts at:      0      92    1052    1575 
   Size:         92     960     523

  Fragments arranged by size: 

                 960     523      92
 
BanI G'GyrC_C

Cuts at:      0     290     403    1575 
   Size:        290     113    1172

  Fragments arranged by size: 

                1172     290     113
 
BanII G_rGCy'C

Cuts at:      0     335     487     959    1028    1087    1575 
   Size:        335     152     472      69      59     488

  Fragments arranged by size: 

                 488     472     335     152      69      59
 
BbeI G_GCGC'C

Cuts at:      0     294    1575 
   Size:        294    1281
 
BbvCI CC'TCA_GC

Cuts at:      0    1432    1559    1575 
   Size:       1432     127      16

  Fragments arranged by size: 

                1432     127      16
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     958     992    1575 
   Size:        958      34     583

  Fragments arranged by size: 

                 958     583      34
 
BglII A'GATC_T

Cuts at:      0     386    1575 
   Size:        386    1189
 
BlpI GC'TnA_GC

Cuts at:      0     943    1575 
   Size:        943     632
 
Bme1580I G_kGCm'C

Cuts at:      0      93     465     839    1575 
   Size:         93     372     374     736

  Fragments arranged by size: 

                 736     374     372      93
 
BmgBI CAC'GTC

Cuts at:      0     204    1575 
   Size:        204    1371
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      80     668     698     975    1117    1575 
   Size:         80     588      30     277     142     458

  Fragments arranged by size: 

                 588     458     277     142      80      30
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     627    1042    1047    1575 
   Size:        627     415       5     528

  Fragments arranged by size: 

                 627     528     415       5
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     262     351     554     730    1071    1243    1575 
   Size:        262      89     203     176     341     172     332

  Fragments arranged by size: 

                 341     332     262     203     176     172      89
 
Bpu10I CC'TnA_GC

Cuts at:      0    1432    1559    1575 
   Size:       1432     127      16

  Fragments arranged by size: 

                1432     127      16
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      64    1575 
   Size:         64    1511
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     169    1575 
   Size:        169    1406
 
BsaHI Gr'CG_yC

Cuts at:      0     291    1300    1575 
   Size:        291    1009     275

  Fragments arranged by size: 

                1009     291     275
 
BsaWI w'CCGG_w

Cuts at:      0     758    1575 
   Size:        758     817
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1057    1087    1575 
   Size:       1057      30     488

  Fragments arranged by size: 

                1057     488      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     244     247     608     616     656     892    1240    1575 
   Size:        244       3     361       8      40     236     348     335

  Fragments arranged by size: 

                 361     348     335     244     236      40       8       3
            
 
BseYI C'CCAG_C

Cuts at:      0     117     664     781    1216    1575 
   Size:        117     547     117     435     359

  Fragments arranged by size: 

                 547     435     359     117     117
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     226     934    1575 
   Size:        226     708     641

  Fragments arranged by size: 

                 708     641     226
 
BsiEI CG_ry'CG

Cuts at:      0    1419    1575 
   Size:       1419     156
 
BsiHKAI G_wGCw'C

Cuts at:      0     568     959    1028    1063    1575 
   Size:        568     391      69      35     512

  Fragments arranged by size: 

                 568     512     391      69      35
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     369    1413    1575 
   Size:        369    1044     162

  Fragments arranged by size: 

                1044     369     162
 
Bsp1286I G_dGCh'C

Cuts at:      0      93     335     465     487     568     839     959    1028 
   Size:         93     242     130      22      81     271     120      69

Cuts at:   1028    1063    1087    1575 
   Size:         35      24     488

  Fragments arranged by size: 

                 488     271     242     130     120      93      81      69
                  35      24      22
 
BspEI T'CCGG_A

Cuts at:      0     758    1575 
   Size:        758     817
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     670    1325    1575 
   Size:        670     655     250

  Fragments arranged by size: 

                 670     655     250
 
BsrBI CCG'CTC

Cuts at:      0     378    1575 
   Size:        378    1197
 
BsrDI GCAATG_nn'

Cuts at:      0     432    1327    1575 
   Size:        432     895     248

  Fragments arranged by size: 

                 895     432     248
 
BsrFI r'CCGG_y

Cuts at:      0    1568    1575 
   Size:       1568       7
 
BssHII G'CGCG_C

Cuts at:      0     150    1575 
   Size:        150    1425
 
BssSI C'ACGA_G

Cuts at:      0     952    1575 
   Size:        952     623
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     460    1575 
   Size:        460    1115
 
BstYI r'GATC_y

Cuts at:      0     386     548     752     881    1575 
   Size:        386     162     204     129     694

  Fragments arranged by size: 

                 694     386     204     162     129
 
Bsu36I CC'TnA_GG

Cuts at:      0     650    1575 
   Size:        650     925
 
BtgI C'CryG_G

Cuts at:      0      98    1338    1575 
   Size:         98    1240     237

  Fragments arranged by size: 

                1240     237      98
 
BtsI GCAGTG_nn'

Cuts at:      0     103     465     514    1575 
   Size:        103     362      49    1061

  Fragments arranged by size: 

                1061     362     103      49
 
EaeI y'GGCC_r

Cuts at:      0     266     553     906    1575 
   Size:        266     287     353     669

  Fragments arranged by size: 

                 669     353     287     266
 
EarI CTCTTCn'nnn_

Cuts at:      0     340     385     593     869    1033    1575 
   Size:        340      45     208     276     164     542

  Fragments arranged by size: 

                 542     340     276     208     164      45
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      56    1575 
   Size:         56    1519
 
EcoICRI GAG'CTC

Cuts at:      0     957    1026    1575 
   Size:        957      69     549

  Fragments arranged by size: 

                 957     549      69
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     262     321     351     554     730    1071    1121    1144 
   Size:        262      59      30     203     176     341      50      23

Cuts at:   1144    1243    1575 
   Size:         99     332

  Fragments arranged by size: 

                 341     332     262     203     176      99      59      50
                  30      23
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     298     533    1181    1225    1502    1575 
   Size:        298     235     648      44     277      73

  Fragments arranged by size: 

                 648     298     277     235      73      44
 
EcoO109I rG'GnC_Cy

Cuts at:      0     141     526     540    1114    1186    1507    1575 
   Size:        141     385      14     574      72     321      68

  Fragments arranged by size: 

                 574     385     321     141      72      68      14
 
HaeII r_GCGC'y

Cuts at:      0      13      56     294    1238    1575 
   Size:         13      43     238     944     337

  Fragments arranged by size: 

                 944     337     238      43      13
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     493     525     778     810    1575 
   Size:        493      32     253      32     765

  Fragments arranged by size: 

                 765     493     253      32      32
 
HincII GTy'rAC

Cuts at:      0     343    1575 
   Size:        343    1232
 
KasI G'GCGC_C

Cuts at:      0     290    1575 
   Size:        290    1285
 
KpnI G_GTAC'C

Cuts at:      0     407    1575 
   Size:        407    1168
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     557    1125    1575 
   Size:        557     568     450

  Fragments arranged by size: 

                 568     557     450
 
MscI TGG'CCA

Cuts at:      0     268     555    1575 
   Size:        268     287    1020

  Fragments arranged by size: 

                1020     287     268
 
MslI CAynn'nnrTG

Cuts at:      0     733    1575 
   Size:        733     842
 
MspA1I CmG'CkG

Cuts at:      0     132     213    1575 
   Size:        132      81    1362

  Fragments arranged by size: 

                1362     132      81
 
NaeI GCC'GGC

Cuts at:      0    1570    1575 
   Size:       1570       5
 
NarI GG'CG_CC

Cuts at:      0     291    1575 
   Size:        291    1284
 
NcoI C'CATG_G

Cuts at:      0      98    1338    1575 
   Size:         98    1240     237

  Fragments arranged by size: 

                1240     237      98
 
NgoMIV G'CCGG_C

Cuts at:      0    1568    1575 
   Size:       1568       7
 
PasI CC'CwG_GG

Cuts at:      0     442    1575 
   Size:        442    1133
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     520    1339    1575 
   Size:        520     819     236

  Fragments arranged by size: 

                 819     520     236
 
PpuMI rG'GwC_Cy

Cuts at:      0     141     526     540    1507    1575 
   Size:        141     385      14     967      68

  Fragments arranged by size: 

                 967     385     141      68      14
 
PshAI GACnn'nnGTC

Cuts at:      0    1493    1575 
   Size:       1493      82
 
PstI C_TGCA'G

Cuts at:      0      71     131     149     299     636    1367    1575 
   Size:         71      60      18     150     337     731     208

  Fragments arranged by size: 

                 731     337     208     150      71      60      18
 
PvuII CAG'CTG

Cuts at:      0     132     213    1575 
   Size:        132      81    1362

  Fragments arranged by size: 

                1362     132      81
 
SacI G_AGCT'C

Cuts at:      0     959    1028    1575 
   Size:        959      69     547

  Fragments arranged by size: 

                 959     547      69
 
SanDI GG'GwC_CC

Cuts at:      0     540    1575 
   Size:        540    1035
 
SapI GCTCTTCn'nnn_

Cuts at:      0     340     385    1033    1575 
   Size:        340      45     648     542

  Fragments arranged by size: 

                 648     542     340      45
 
SbfI CC_TGCA'GG

Cuts at:      0     636    1575 
   Size:        636     939
 
SfcI C'TryA_G

Cuts at:      0      67     127     145     295     632    1363    1575 
   Size:         67      60      18     150     337     731     212

  Fragments arranged by size: 

                 731     337     212     150      67      60      18
 
SfoI GGC'GCC

Cuts at:      0     292    1575 
   Size:        292    1283
 
SmlI C'TyrA_G

Cuts at:      0      79    1575 
   Size:         79    1496
 
StyI C'CwwG_G

Cuts at:      0      98     769    1291    1338    1575 
   Size:         98     671     522      47     237

  Fragments arranged by size: 

                 671     522     237      98      47
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     719    1575 
   Size:        719     856
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     678     710    1575 
   Size:        678      32     865

  Fragments arranged by size: 

                 865     678      32
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI    AlwNI     AvaI     BanI    BanII     BbeI
    BbvCI     BcgI    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspMI
    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstYI   Bsu36I
     BtgI     BtsI     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    HaeII    Hin4I   HincII     KasI     KpnI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     PasI    PflMI
    PpuMI    PshAI     PstI    PvuII     SacI    SanDI     SapI     SbfI
     SfcI     SfoI     SmlI     StyI    TaqII     TstI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BbsI    BciVI     BclI    BfrBI
     BglI     BmtI    BsaAI    BsaBI    BsiWI     BsmI    BspHI    BsrGI
    BstBI   BstEII    BstXI  BstZ17I    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI  HindIII     HpaI     MfeI     MluI     NdeI     NheI     NotI
     NruI     NsiI     NspI     PacI     PciI     PfoI     PmeI     PmlI
     PpiI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII
     SalI     ScaI    SexAI     SfiI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI     TatI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI
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