Restriction map for pF1KE0206
##################################
# NO CUT ENZYMES (  165 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwNI       AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
AvaI        AviII       AvrII       BalI        BamHI       
BbeI        BbrPI       BcgI        BciVI       BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BsaAI       BsaBI       BsaHI       BsaWI       
BseAI       BsgI        BsiEI       BsiWI       BsmBI       
BsmI        BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstF5I      BstPI       BstZ17I     Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DraIII      
DrdI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT22I     FauI        FokI        
FseI        FspI        HaeII       HgaI        Hin1I       
HincII      HindII      HpaI        KasI        KpnI        
KspI        MaeI        MaeIII      MamI        MfeI        
MluI        MluNI       MroI        MscI        MspA1I      
MunI        NaeI        NarI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsaI        RsrII       SacII       
SalI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        TfiI        Tsp45I      Tth111I     
Van91I      XbaI        XhoI        XmaCI       XmaI        


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# ONE CUT ENZYMES (   47 enzymes) #
###################################

AccII         342  CGCG                 CG^CG
Asp700I       510  GAAAGGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI          89  GAGCTC               G[AT]GC[AT]^C
BanII          89  GAGCTC               G[GA]GC[CT]^C
BbsI          127  GTCTTC               GAAGAC(2/6)
BbvCI         260  CCTCAGC              CCTCAGC(-5/-2)
BbvI          598  GCAGC                GCAGC(8/12)
BclI          141  TGATCA               T^GATCA
BcnI          400  CCCGG                CC^[GC]GG
BpuAI         127  GTCTTC               GAAGAC(2/6)
BsiHKAI        89  GAGCTC               G[AT]GC[AT]^C
BsrDI         200  GCAATG               GCAATG(2/0)
BsrI          375  ACTGG                ACTGG(1/-1)
BstAPI        224  GCAACCTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI         342  CGCG                 CG^CG
BstXI          59  CCAAAGTGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         152  AGATCC               [GA]^GATC[CT]
Cac8I         115  GCCAGC               GC[ACGT]^[ACGT]GC
DraI          163  TTTAAA               TTT^AAA
DraII         395  AGGCCCC              [GA]G^G[ACGT]CC[CT]
DsaI          383  CCATGG               C^C[GA][CT]GG
EcoO109I      395  AGGCCCC              [GA]G^G[ACGT]CC[CT]
EcoT14I       383  CCATGG               C^C[AT][AT]GG
FbaI          141  TGATCA               T^GATCA
HapII         401  CCGG                 C^CGG
HindIII       272  AAGCTT               A^AGCTT
HinfI         171  GAGTC                G^A[ACGT]TC
HpaII         401  CCGG                 C^CGG
MflI          152  AGATCC               [GA]^GATC[CT]
MseI          164  TTAA                 T^TAA
MslI          279  CACCATCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI          401  CCGG                 C^CGG
MvnI          342  CGCG                 CG^CG
NciI          400  CCCGG                CC^[GC]GG
NcoI          383  CCATGG               C^CATGG
PleI          171  GAGTC                GAGTC(4/5)
SacI           89  GAGCTC               GAGCT^C
SapI           86  GAAGAGC              GCTCTTC(1/4)
SfaNI         353  GCATC                GCATC(5/9)
SfcI          633  CTACAG               C^T[GA][CT]AG
StyI          383  CCATGG               C^C[AT][AT]GG
Tru9I         164  TTAA                 T^TAA
TseI          598  GCAGC                G^C[AT]GC
Tsp509I       250  AATT                 ^AATT
XcmI          291  CCAGAACCGTGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         152  AGATCC               [GA]^GATC[CT]
XmnI          510  GAAAGGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AlwI           39  GATCC                GGATC(4/5)
              153  GATCC               
BanI           78  GGCACC               G^G[CT][GA]CC
              109  GGCACC              
BmyI           89  GAGCTC               G[AGT]GC[ACT]^C
              453  GGGCAC              
BpmI          193  CTGGAG               CTGGAG(16/14)
              376  CTGGAG              
BsaI          469  GAGACC               GGTCTC(1/5)
               15  GGTCTC              
BsiYI         150  CCAGATCCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              431  CCATGTTTGGG         
BslI          150  CCAGATCCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              431  CCATGTTTGGG         
BsmAI         469  GAGAC                GTCTC(1/5)
               16  GTCTC               
BsmFI         329  GGGAC                GGGAC(10/14)
              147  GTCCC               
Bsp1286I       89  GAGCTC               G[AGT]GC[ACT]^C
              453  GGGCAC              
CfoI          198  GCGC                 GCG^C
              343  GCGC                
DdeI            7  CTTAG                C^T[ACGT]AG
              261  CTCAG               
EarI           86  GAAGAG               CTCTTC(1/4)
              667  GAAGAG              
HhaI          198  GCGC                 GCG^C
              343  GCGC                
HinP1I        198  GCGC                 G^CGC
              343  GCGC                
Ksp632I        86  GAAGAG               CTCTTC(1/4)
              667  GAAGAG              
MaeII         125  ACGT                 A^CGT
              650  ACGT                
SmlI           32  CTCAAG               C^T[CT][GA]AG
              324  CTTGAG              
TaqI          311  TCGA                 T^CGA
              497  TCGA                
TthHB8I       311  TCGA                 T^CGA
              497  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   26 enzymes) #
#########################################

AciI        AluI        AvaII       BsaJI       BseRI       
BssKI       BstNI       Cfr13I      DpnII       EcoRII      
Fnu4HI      HaeIII      HphI        ItaI        MboI        
MboII       MnlI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       Sau3AI      Sau96I      ScrFI       
TspRI       
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