Restriction map for pF1KE0206
 (Linear) (Six-base) MAPSORT of: KIEE0206.seq  Check: 2219  from: 1  to: 678



KIEE0206.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:11  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     469     524     552     678 
   Size:        469      55      28     126

  Fragments arranged by size: 

                 469     126      55      28
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     611     678 
   Size:        611      67
 
BanI G'GyrC_C

Cuts at:      0      78     109     678 
   Size:         78      31     569

  Fragments arranged by size: 

                 569      78      31
 
BanII G_rGCy'C

Cuts at:      0      93     678 
   Size:         93     585
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     120     678 
   Size:        120     558
 
BbvCI CC'TCA_GC

Cuts at:      0     261     678 
   Size:        261     417
 
BclI T'GATC_A

Cuts at:      0     141     678 
   Size:        141     537
 
Bme1580I G_kGCm'C

Cuts at:      0     457     678 
   Size:        457     221
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     107     194     678 
   Size:        107      87     484

  Fragments arranged by size: 

                 484     107      87
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     214     397     678 
   Size:        214     183     281

  Fragments arranged by size: 

                 281     214     183
 
Bpu10I CC'TnA_GC

Cuts at:      0       7     261     678 
   Size:          7     254     417

  Fragments arranged by size: 

                 417     254       7
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      17     345     678 
   Size:         17     328     333

  Fragments arranged by size: 

                 333     328      17
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      21     463     678 
   Size:         21     442     215

  Fragments arranged by size: 

                 442     215      21
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     115     145     678 
   Size:        115      30     533

  Fragments arranged by size: 

                 533     115      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      38     481     589     592     678 
   Size:         38     443     108       3      86

  Fragments arranged by size: 

                 443     108      86      38       3
 
BseYI C'CCAG_C

Cuts at:      0      66     345     678 
   Size:         66     279     333

  Fragments arranged by size: 

                 333     279      66
 
BsiHKAI G_wGCw'C

Cuts at:      0      93     678 
   Size:         93     585
 
Bsp1286I G_dGCh'C

Cuts at:      0      93     457     678 
   Size:         93     364     221

  Fragments arranged by size: 

                 364     221      93
 
BsrDI GCAATG_nn'

Cuts at:      0     207     678 
   Size:        207     471
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     230     678 
   Size:        230     448
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      66     678 
   Size:         66     612
 
BstYI r'GATC_y

Cuts at:      0     152     678 
   Size:        152     526
 
BtgI C'CryG_G

Cuts at:      0     383     678 
   Size:        383     295
 
BtsI GCAGTG_nn'

Cuts at:      0     219     678 
   Size:        219     459
 
DraI TTT'AAA

Cuts at:      0     165     678 
   Size:        165     513
 
EarI CTCTTCn'nnn_

Cuts at:      0      81     662     678 
   Size:         81     581      16

  Fragments arranged by size: 

                 581      81      16
 
EcoICRI GAG'CTC

Cuts at:      0      91     678 
   Size:         91     587
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     214     397     469     524     552     678 
   Size:        214     183      72      55      28     126

  Fragments arranged by size: 

                 214     183     126      72      55      28
 
EcoO109I rG'GnC_Cy

Cuts at:      0     396     678 
   Size:        396     282
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     562     678 
   Size:        562     116
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      30      62     201     233     678 
   Size:         30      32     139      32     445

  Fragments arranged by size: 

                 445     139      32      32      30
 
HindIII A'AGCT_T

Cuts at:      0     272     678 
   Size:        272     406
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     146     678 
   Size:        146     532
 
MslI CAynn'nnrTG

Cuts at:      0     283     678 
   Size:        283     395
 
NcoI C'CATG_G

Cuts at:      0     383     678 
   Size:        383     295
 
PfoI T'CCnGG_A

Cuts at:      0     155     678 
   Size:        155     523
 
SacI G_AGCT'C

Cuts at:      0      93     678 
   Size:         93     585
 
SapI GCTCTTCn'nnn_

Cuts at:      0      81     678 
   Size:         81     597
 
SfcI C'TryA_G

Cuts at:      0     633     678 
   Size:        633      45
 
SmlI C'TyrA_G

Cuts at:      0      32     324     678 
   Size:         32     292     354

  Fragments arranged by size: 

                 354     292      32
 
StyI C'CwwG_G

Cuts at:      0     383     678 
   Size:        383     295
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     298     678 
   Size:        298     380
 
XmnI GAAnn'nnTTC

Cuts at:      0     514     678 
   Size:        514     164
 

 Enzymes that do cut:

     AcuI     AlfI     BanI    BanII     BbsI    BbvCI     BclI Bme1580I
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaXI    BseRI    BseYI
  BsiHKAI Bsp1286I    BsrDI   BstAPI    BstXI    BstYI     BtgI     BtsI
     DraI     EarI  EcoICRI  Eco57MI EcoO109I     FalI    Hin4I  HindIII
     MmeI     MslI     NcoI     PfoI     SacI     SapI     SfcI     SmlI
     StyI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI
     BcgI    BciVI    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI
     BmtI    BsaAI    BsaBI    BsaHI    BsaWI     BsgI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI
   BssHII    BssSI    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     ClaI
    CspCI   DraIII     DrdI     EaeI     EagI     EciI    EcoNI    EcoRI
    EcoRV     FseI     FspI    FspAI    HaeII   HincII     HpaI     KasI
     KpnI     MfeI     MluI     MscI   MspA1I     NaeI     NarI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII    SacII
     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmaI
     ZraI
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