(Circular) (Six-base) MAPSORT of: pF1KE0533.seq Check: 31 from: 1 to: 3731 pF1KE0533 3731 bp With 182 enzymes: SgfI * April 24, 2009 13:15 .. AccI GT'mk_AC Cuts at: 769 769 Size: 3731 Acc65I G'GTAC_C Cuts at: 747 747 Size: 3731 AclI AA'CG_TT Cuts at: 3571 3571 Size: 3731 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 481 692 1556 1988 2735 3003 481 Size: 211 864 432 747 268 1209 Fragments arranged by size: 1209 864 747 432 268 211 AfeI AGC'GCT Cuts at: 307 307 Size: 3731 AflIII A'CryG_T Cuts at: 1083 2187 3164 1083 Size: 1104 977 1650 Fragments arranged by size: 1650 1104 977 AgeI A'CCGG_T Cuts at: 2119 2119 Size: 3731 AleI CACnn'nnGTG Cuts at: 697 709 697 Size: 12 3719 AlwNI CAG_nnn'CTG Cuts at: 1036 2603 1036 Size: 1567 2164 ApaLI G'TGCA_C Cuts at: 2501 2501 Size: 3731 ApoI r'AATT_y Cuts at: 735 3084 3340 735 Size: 2349 256 1126 Fragments arranged by size: 2349 1126 256 AseI AT'TA_AT Cuts at: 20 20 Size: 3731 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 3731 AvaI C'yCGr_G Cuts at: 751 751 Size: 3731 BamHI G'GATC_C Cuts at: 600 756 600 Size: 156 3575 BanI G'GyrC_C Cuts at: 747 1161 1409 1444 747 Size: 414 248 35 3034 Fragments arranged by size: 3034 414 248 35 BanII G_rGCy'C Cuts at: 425 453 745 1775 425 Size: 28 292 1030 2381 Fragments arranged by size: 2381 1030 292 28 BbeI G_GCGC'C Cuts at: 1165 1413 1165 Size: 248 3483 BbvCI CC'TCA_GC Cuts at: 584 584 Size: 3731 BciVI GTATCCnnnnn_n' Cuts at: 1629 2390 3731 1629 Size: 761 1341 1629 Fragments arranged by size: 1629 1341 761 BclI T'GATC_A Cuts at: 1251 1251 Size: 3731 BglI GCCn_nnn'nGGC Cuts at: 112 112 Size: 3731 BglII A'GATC_T Cuts at: 1246 3283 1246 Size: 2037 1694 BlpI GC'TnA_GC Cuts at: 843 843 Size: 3731 Bme1580I G_kGCm'C Cuts at: 1356 1449 2505 1356 Size: 93 1056 2582 Fragments arranged by size: 2582 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 943 1024 1359 3503 943 Size: 81 335 2144 1171 Fragments arranged by size: 2144 1171 335 81 BmtI G_CTAG'C Cuts at: 142 142 Size: 3731 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 467 2098 467 Size: 1631 2100 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 568 568 Size: 3731 Bpu10I CC'TnA_GC Cuts at: 584 584 Size: 3731 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 329 907 1786 2278 2576 329 Size: 578 879 492 298 1484 Fragments arranged by size: 1484 879 578 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 3435 3435 Size: 3731 BsaAI yAC'GTr Cuts at: 1084 1084 Size: 3731 BsaBI GATnn'nnATC Cuts at: 6 1245 3282 6 Size: 1239 2037 455 Fragments arranged by size: 2037 1239 455 BsaHI Gr'CG_yC Cuts at: 1162 1410 2112 3608 1162 Size: 248 702 1496 1285 Fragments arranged by size: 1496 1285 702 248 BsaWI w'CCGG_w Cuts at: 920 1441 2119 2393 2540 2951 920 Size: 521 678 274 147 411 1700 Fragments arranged by size: 1700 678 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 448 459 448 Size: 11 3720 BseYI C'CCAG_C Cuts at: 533 1156 1914 2491 2987 533 Size: 623 758 577 496 1277 Fragments arranged by size: 1277 758 623 577 496 BsiEI CG_ry'CG Cuts at: 104 1273 1319 2527 104 Size: 1169 46 1208 1308 Fragments arranged by size: 1308 1208 1169 46 BsiHKAI G_wGCw'C Cuts at: 425 453 745 1523 1713 2505 425 Size: 28 292 778 190 792 1651 Fragments arranged by size: 1651 792 778 292 190 28 Bsp1286I G_dGCh'C Cuts at: 425 453 745 1356 1449 1523 1713 1775 2505 Size: 28 292 611 93 74 190 62 730 Cuts at: 2505 425 Size: 1651 Fragments arranged by size: 1651 730 611 292 190 93 74 62 28 BspEI T'CCGG_A Cuts at: 2951 2951 Size: 3731 BspHI T'CATG_A Cuts at: 2907 2907 Size: 3731 BspMI ACCTGCnnnn'nnnn_ Cuts at: 668 781 995 1297 1678 668 Size: 113 214 302 381 2721 Fragments arranged by size: 2721 381 302 214 113 BsrBI CCG'CTC Cuts at: 50 408 2023 2077 3561 3726 50 Size: 358 1615 54 1484 165 55 Fragments arranged by size: 1615 1484 358 165 55 54 BsrDI GCAATG_nn' Cuts at: 1643 1643 Size: 3731 BsrFI r'CCGG_y Cuts at: 1729 1910 2119 1729 Size: 181 209 3341 Fragments arranged by size: 3341 209 181 BsrGI T'GTAC_A Cuts at: 476 701 476 Size: 225 3506 BssSI C'ACGA_G Cuts at: 2002 2360 2002 Size: 358 3373 BstAPI GCAn_nnn'nTGC Cuts at: 1108 1108 Size: 3731 BstBI TT'CG_AA Cuts at: 258 2092 258 Size: 1834 1897 BstYI r'GATC_y Cuts at: 1 276 320 600 756 1223 1246 1581 1827 Size: 275 44 280 156 467 23 335 246 Cuts at: 1827 2839 2925 2937 3283 1 Size: 1012 86 12 346 449 Fragments arranged by size: 1012 467 449 346 335 280 275 246 156 86 44 23 12 Bsu36I CC'TnA_GG Cuts at: 413 413 Size: 3731 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 1681 1862 2025 1681 Size: 181 163 3387 Fragments arranged by size: 3387 181 163 BtsI GCAGTG_nn' Cuts at: 974 974 Size: 3731 DraI TTT'AAA Cuts at: 730 730 Size: 3731 DrdI GACnn_nn'nnGTC Cuts at: 1437 2295 1437 Size: 858 2873 EaeI y'GGCC_r Cuts at: 1316 1490 1881 1908 3253 1316 Size: 174 391 27 1345 1794 Fragments arranged by size: 1794 1345 391 174 27 EagI C'GGCC_G Cuts at: 1316 1316 Size: 3731 EarI CTCTTCn'nnn_ Cuts at: 260 1754 1964 260 Size: 1494 210 2027 Fragments arranged by size: 2027 1494 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 1939 2249 2395 3541 1939 Size: 310 146 1146 2129 Fragments arranged by size: 2129 1146 310 146 EcoICRI GAG'CTC Cuts at: 423 451 743 423 Size: 28 292 3411 Fragments arranged by size: 3411 292 28 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 481 568 692 1556 1988 2735 3003 481 Size: 87 124 864 432 747 268 1209 Fragments arranged by size: 1209 864 747 432 268 124 87 EcoO109I rG'GnC_Cy Cuts at: 870 870 Size: 3731 EcoRI G'AATT_C Cuts at: 735 735 Size: 3731 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 324 489 1750 324 Size: 165 1261 2305 Fragments arranged by size: 2305 1261 165 FspI TGC'GCA Cuts at: 1512 1512 Size: 3731 HaeII r_GCGC'y Cuts at: 309 1165 1413 2435 3414 309 Size: 856 248 1022 979 626 Fragments arranged by size: 1022 979 856 626 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1573 1603 1605 1635 1573 Size: 30 2 30 3669 Fragments arranged by size: 3669 30 30 2 HincII GTy'rAC Cuts at: 770 770 Size: 3731 KasI G'GCGC_C Cuts at: 1161 1409 1161 Size: 248 3483 KpnI G_GTAC'C Cuts at: 751 751 Size: 3731 MluI A'CGCG_T Cuts at: 3164 3164 Size: 3731 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2402 2586 2402 Size: 184 3547 MscI TGG'CCA Cuts at: 1492 1492 Size: 3731 MslI CAynn'nnrTG Cuts at: 238 697 709 1720 1847 238 Size: 459 12 1011 127 2122 Fragments arranged by size: 2122 1011 459 127 12 MspA1I CmG'CkG Cuts at: 842 1156 1516 2529 2774 842 Size: 314 360 1013 245 1799 Fragments arranged by size: 1799 1013 360 314 245 NaeI GCC'GGC Cuts at: 1912 1912 Size: 3731 NarI GG'CG_CC Cuts at: 1162 1410 1162 Size: 248 3483 NgoMIV G'CCGG_C Cuts at: 1910 1910 Size: 3731 NheI G'CTAG_C Cuts at: 138 138 Size: 3731 NspI r_CATG'y Cuts at: 784 1284 2191 784 Size: 500 907 2324 Fragments arranged by size: 2324 907 500 PciI A'CATG_T Cuts at: 2187 2187 Size: 3731 PmeI GTTT'AAAC Cuts at: 730 730 Size: 3731 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2870 2902 2870 Size: 32 3699 PstI C_TGCA'G Cuts at: 778 1463 778 Size: 685 3046 PvuI CG_AT'CG Cuts at: 104 104 Size: 3731 PvuII CAG'CTG Cuts at: 1156 1516 1156 Size: 360 3371 RsrII CG'GwC_CG Cuts at: 1926 1926 Size: 3731 SacI G_AGCT'C Cuts at: 425 453 745 425 Size: 28 292 3411 Fragments arranged by size: 3411 292 28 SalI G'TCGA_C Cuts at: 768 768 Size: 3731 SapI GCTCTTCn'nnn_ Cuts at: 1754 1964 1754 Size: 210 3521 SbfI CC_TGCA'GG Cuts at: 778 778 Size: 3731 ScaI AGT'ACT Cuts at: 3248 3248 Size: 3731 SfcI C'TryA_G Cuts at: 33 774 1459 2452 2643 33 Size: 741 685 993 191 1121 Fragments arranged by size: 1121 993 741 685 191 SfoI GGC'GCC Cuts at: 1163 1411 1163 Size: 248 3483 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 3731 SmaI CCC'GGG Cuts at: 753 753 Size: 3731 SmlI C'TyrA_G Cuts at: 344 886 1801 2293 2555 344 Size: 542 915 492 262 1520 Fragments arranged by size: 1520 915 542 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 3731 SphI G_CATG'C Cuts at: 784 1284 784 Size: 500 3231 StyI C'CwwG_G Cuts at: 695 865 1219 695 Size: 170 354 3207 Fragments arranged by size: 3207 354 170 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1314 1447 1906 2115 1314 Size: 133 459 209 2930 Fragments arranged by size: 2930 459 209 133 TatI w'GTAC_w Cuts at: 476 701 3246 476 Size: 225 2545 961 Fragments arranged by size: 2545 961 225 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 635 667 3248 3280 635 Size: 32 2581 32 1086 Fragments arranged by size: 2581 1086 32 32 Tth111I GACn'n_nGTC Cuts at: 1528 1528 Size: 3731 XbaI T'CTAG_A Cuts at: 762 762 Size: 3731 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1226 1226 Size: 3731 XmaI C'CCGG_G Cuts at: 751 751 Size: 3731 XmnI GAAnn'nnTTC Cuts at: 233 254 233 Size: 21 3710 Enzymes that do cut: AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI Bsu36I BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NheI NspI PciI PmeI PpiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AflII AhdI AlfI AloI ApaI AscI AvrII BaeI BbsI BcgI BfrBI BmgBI BsaXI BsgI BsiWI BsmI BsmBI BssHII BstEII BstXI BstZ17I BtgI ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI NotI NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI