Restriction map for pF1KE0533
 (Circular) (Six-base) MAPSORT of: pF1KE0533.seq  Check: 31  from: 1  to: 3731



pF1KE0533  3731 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:15  ..

AccI GT'mk_AC

Cuts at:    769     769 
   Size:       3731
 
Acc65I G'GTAC_C

Cuts at:    747     747 
   Size:       3731
 
AclI AA'CG_TT

Cuts at:   3571    3571 
   Size:       3731
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    481     692    1556    1988    2735    3003     481 
   Size:        211     864     432     747     268    1209

  Fragments arranged by size: 

                1209     864     747     432     268     211
 
AfeI AGC'GCT

Cuts at:    307     307 
   Size:       3731
 
AflIII A'CryG_T

Cuts at:   1083    2187    3164    1083 
   Size:       1104     977    1650

  Fragments arranged by size: 

                1650    1104     977
 
AgeI A'CCGG_T

Cuts at:   2119    2119 
   Size:       3731
 
AleI CACnn'nnGTG

Cuts at:    697     709     697 
   Size:         12    3719
 
AlwNI CAG_nnn'CTG

Cuts at:   1036    2603    1036 
   Size:       1567    2164
 
ApaLI G'TGCA_C

Cuts at:   2501    2501 
   Size:       3731
 
ApoI r'AATT_y

Cuts at:    735    3084    3340     735 
   Size:       2349     256    1126

  Fragments arranged by size: 

                2349    1126     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3731
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3731
 
AvaI C'yCGr_G

Cuts at:    751     751 
   Size:       3731
 
BamHI G'GATC_C

Cuts at:    600     756     600 
   Size:        156    3575
 
BanI G'GyrC_C

Cuts at:    747    1161    1409    1444     747 
   Size:        414     248      35    3034

  Fragments arranged by size: 

                3034     414     248      35
 
BanII G_rGCy'C

Cuts at:    425     453     745    1775     425 
   Size:         28     292    1030    2381

  Fragments arranged by size: 

                2381    1030     292      28
 
BbeI G_GCGC'C

Cuts at:   1165    1413    1165 
   Size:        248    3483
 
BbvCI CC'TCA_GC

Cuts at:    584     584 
   Size:       3731
 
BciVI GTATCCnnnnn_n'

Cuts at:   1629    2390    3731    1629 
   Size:        761    1341    1629

  Fragments arranged by size: 

                1629    1341     761
 
BclI T'GATC_A

Cuts at:   1251    1251 
   Size:       3731
 
BglI GCCn_nnn'nGGC

Cuts at:    112     112 
   Size:       3731
 
BglII A'GATC_T

Cuts at:   1246    3283    1246 
   Size:       2037    1694
 
BlpI GC'TnA_GC

Cuts at:    843     843 
   Size:       3731
 
Bme1580I G_kGCm'C

Cuts at:   1356    1449    2505    1356 
   Size:         93    1056    2582

  Fragments arranged by size: 

                2582    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    943    1024    1359    3503     943 
   Size:         81     335    2144    1171

  Fragments arranged by size: 

                2144    1171     335      81
 
BmtI G_CTAG'C

Cuts at:    142     142 
   Size:       3731
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    467    2098     467 
   Size:       1631    2100
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    568     568 
   Size:       3731
 
Bpu10I CC'TnA_GC

Cuts at:    584     584 
   Size:       3731
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    329     907    1786    2278    2576     329 
   Size:        578     879     492     298    1484

  Fragments arranged by size: 

                1484     879     578     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3435    3435 
   Size:       3731
 
BsaAI yAC'GTr

Cuts at:   1084    1084 
   Size:       3731
 
BsaBI GATnn'nnATC

Cuts at:      6    1245    3282       6 
   Size:       1239    2037     455

  Fragments arranged by size: 

                2037    1239     455
 
BsaHI Gr'CG_yC

Cuts at:   1162    1410    2112    3608    1162 
   Size:        248     702    1496    1285

  Fragments arranged by size: 

                1496    1285     702     248
 
BsaWI w'CCGG_w

Cuts at:    920    1441    2119    2393    2540    2951     920 
   Size:        521     678     274     147     411    1700

  Fragments arranged by size: 

                1700     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    448     459     448 
   Size:         11    3720
 
BseYI C'CCAG_C

Cuts at:    533    1156    1914    2491    2987     533 
   Size:        623     758     577     496    1277

  Fragments arranged by size: 

                1277     758     623     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    1273    1319    2527     104 
   Size:       1169      46    1208    1308

  Fragments arranged by size: 

                1308    1208    1169      46
 
BsiHKAI G_wGCw'C

Cuts at:    425     453     745    1523    1713    2505     425 
   Size:         28     292     778     190     792    1651

  Fragments arranged by size: 

                1651     792     778     292     190      28
 
Bsp1286I G_dGCh'C

Cuts at:    425     453     745    1356    1449    1523    1713    1775    2505 
   Size:         28     292     611      93      74     190      62     730

Cuts at:   2505     425 
   Size:       1651

  Fragments arranged by size: 

                1651     730     611     292     190      93      74      62
                  28
 
BspEI T'CCGG_A

Cuts at:   2951    2951 
   Size:       3731
 
BspHI T'CATG_A

Cuts at:   2907    2907 
   Size:       3731
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    668     781     995    1297    1678     668 
   Size:        113     214     302     381    2721

  Fragments arranged by size: 

                2721     381     302     214     113
 
BsrBI CCG'CTC

Cuts at:     50     408    2023    2077    3561    3726      50 
   Size:        358    1615      54    1484     165      55

  Fragments arranged by size: 

                1615    1484     358     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1643    1643 
   Size:       3731
 
BsrFI r'CCGG_y

Cuts at:   1729    1910    2119    1729 
   Size:        181     209    3341

  Fragments arranged by size: 

                3341     209     181
 
BsrGI T'GTAC_A

Cuts at:    476     701     476 
   Size:        225    3506
 
BssSI C'ACGA_G

Cuts at:   2002    2360    2002 
   Size:        358    3373
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1108    1108 
   Size:       3731
 
BstBI TT'CG_AA

Cuts at:    258    2092     258 
   Size:       1834    1897
 
BstYI r'GATC_y

Cuts at:      1     276     320     600     756    1223    1246    1581    1827 
   Size:        275      44     280     156     467      23     335     246

Cuts at:   1827    2839    2925    2937    3283       1 
   Size:       1012      86      12     346     449

  Fragments arranged by size: 

                1012     467     449     346     335     280     275     246
                 156      86      44      23      12
 
Bsu36I CC'TnA_GG

Cuts at:    413     413 
   Size:       3731
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1681    1862    2025    1681 
   Size:        181     163    3387

  Fragments arranged by size: 

                3387     181     163
 
BtsI GCAGTG_nn'

Cuts at:    974     974 
   Size:       3731
 
DraI TTT'AAA

Cuts at:    730     730 
   Size:       3731
 
DrdI GACnn_nn'nnGTC

Cuts at:   1437    2295    1437 
   Size:        858    2873
 
EaeI y'GGCC_r

Cuts at:   1316    1490    1881    1908    3253    1316 
   Size:        174     391      27    1345    1794

  Fragments arranged by size: 

                1794    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1316    1316 
   Size:       3731
 
EarI CTCTTCn'nnn_

Cuts at:    260    1754    1964     260 
   Size:       1494     210    2027

  Fragments arranged by size: 

                2027    1494     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1939    2249    2395    3541    1939 
   Size:        310     146    1146    2129

  Fragments arranged by size: 

                2129    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    423     451     743     423 
   Size:         28     292    3411

  Fragments arranged by size: 

                3411     292      28
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    481     568     692    1556    1988    2735    3003     481 
   Size:         87     124     864     432     747     268    1209

  Fragments arranged by size: 

                1209     864     747     432     268     124      87
 
EcoO109I rG'GnC_Cy

Cuts at:    870     870 
   Size:       3731
 
EcoRI G'AATT_C

Cuts at:    735     735 
   Size:       3731
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    324     489    1750     324 
   Size:        165    1261    2305

  Fragments arranged by size: 

                2305    1261     165
 
FspI TGC'GCA

Cuts at:   1512    1512 
   Size:       3731
 
HaeII r_GCGC'y

Cuts at:    309    1165    1413    2435    3414     309 
   Size:        856     248    1022     979     626

  Fragments arranged by size: 

                1022     979     856     626     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1573    1603    1605    1635    1573 
   Size:         30       2      30    3669

  Fragments arranged by size: 

                3669      30      30       2
 
HincII GTy'rAC

Cuts at:    770     770 
   Size:       3731
 
KasI G'GCGC_C

Cuts at:   1161    1409    1161 
   Size:        248    3483
 
KpnI G_GTAC'C

Cuts at:    751     751 
   Size:       3731
 
MluI A'CGCG_T

Cuts at:   3164    3164 
   Size:       3731
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2402    2586    2402 
   Size:        184    3547
 
MscI TGG'CCA

Cuts at:   1492    1492 
   Size:       3731
 
MslI CAynn'nnrTG

Cuts at:    238     697     709    1720    1847     238 
   Size:        459      12    1011     127    2122

  Fragments arranged by size: 

                2122    1011     459     127      12
 
MspA1I CmG'CkG

Cuts at:    842    1156    1516    2529    2774     842 
   Size:        314     360    1013     245    1799

  Fragments arranged by size: 

                1799    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   1912    1912 
   Size:       3731
 
NarI GG'CG_CC

Cuts at:   1162    1410    1162 
   Size:        248    3483
 
NgoMIV G'CCGG_C

Cuts at:   1910    1910 
   Size:       3731
 
NheI G'CTAG_C

Cuts at:    138     138 
   Size:       3731
 
NspI r_CATG'y

Cuts at:    784    1284    2191     784 
   Size:        500     907    2324

  Fragments arranged by size: 

                2324     907     500
 
PciI A'CATG_T

Cuts at:   2187    2187 
   Size:       3731
 
PmeI GTTT'AAAC

Cuts at:    730     730 
   Size:       3731
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2870    2902    2870 
   Size:         32    3699
 
PstI C_TGCA'G

Cuts at:    778    1463     778 
   Size:        685    3046
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3731
 
PvuII CAG'CTG

Cuts at:   1156    1516    1156 
   Size:        360    3371
 
RsrII CG'GwC_CG

Cuts at:   1926    1926 
   Size:       3731
 
SacI G_AGCT'C

Cuts at:    425     453     745     425 
   Size:         28     292    3411

  Fragments arranged by size: 

                3411     292      28
 
SalI G'TCGA_C

Cuts at:    768     768 
   Size:       3731
 
SapI GCTCTTCn'nnn_

Cuts at:   1754    1964    1754 
   Size:        210    3521
 
SbfI CC_TGCA'GG

Cuts at:    778     778 
   Size:       3731
 
ScaI AGT'ACT

Cuts at:   3248    3248 
   Size:       3731
 
SfcI C'TryA_G

Cuts at:     33     774    1459    2452    2643      33 
   Size:        741     685     993     191    1121

  Fragments arranged by size: 

                1121     993     741     685     191
 
SfoI GGC'GCC

Cuts at:   1163    1411    1163 
   Size:        248    3483
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3731
 
SmaI CCC'GGG

Cuts at:    753     753 
   Size:       3731
 
SmlI C'TyrA_G

Cuts at:    344     886    1801    2293    2555     344 
   Size:        542     915     492     262    1520

  Fragments arranged by size: 

                1520     915     542     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3731
 
SphI G_CATG'C

Cuts at:    784    1284     784 
   Size:        500    3231
 
StyI C'CwwG_G

Cuts at:    695     865    1219     695 
   Size:        170     354    3207

  Fragments arranged by size: 

                3207     354     170
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1314    1447    1906    2115    1314 
   Size:        133     459     209    2930

  Fragments arranged by size: 

                2930     459     209     133
 
TatI w'GTAC_w

Cuts at:    476     701    3246     476 
   Size:        225    2545     961

  Fragments arranged by size: 

                2545     961     225
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    635     667    3248    3280     635 
   Size:         32    2581      32    1086

  Fragments arranged by size: 

                2581    1086      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1528    1528 
   Size:       3731
 
XbaI T'CTAG_A

Cuts at:    762     762 
   Size:       3731
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1226    1226 
   Size:       3731
 
XmaI C'CCGG_G

Cuts at:    751     751 
   Size:       3731
 
XmnI GAAnn'nnTTC

Cuts at:    233     254     233 
   Size:         21    3710
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI     AleI
    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI    BbvCI    BciVI     BclI     BglI    BglII     BlpI
 Bme1580I     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BseRI    BseYI    BsiEI  BsiHKAI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
    BssSI   BstAPI    BstBI    BstYI   Bsu36I    BtgZI     BtsI     DraI
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I
    EcoRI     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV
     NheI     NspI     PciI     PmeI     PpiI     PstI     PvuI    PvuII
    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI     SfoI
     SgfI     SmaI     SmlI     SpeI     SphI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII    AflII     AhdI     AlfI     AloI     ApaI     AscI
    AvrII     BaeI     BbsI     BcgI    BfrBI    BmgBI    BsaXI     BsgI
    BsiWI     BsmI    BsmBI   BssHII   BstEII    BstXI  BstZ17I     BtgI
     ClaI    CspCI   DraIII    EcoNI    EcoRV     FseI    FspAI  HindIII
     HpaI     MfeI     NcoI     NdeI     NotI     NruI     NsiI     PacI
     PasI    PflMI     PfoI     PmlI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI
     SrfI     SspI     StuI     SwaI     XhoI     ZraI
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