Restriction map for pF1KB7952
 (Linear) (Six-base) MAPSORT of: KIBB7952.seq  Check: 2046  from: 1  to: 1182



KIBB7952.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:28  ..

Acc65I G'GTAC_C

Cuts at:      0     926    1182 
   Size:        926     256
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     481     855     939    1182 
   Size:        481     374      84     243

  Fragments arranged by size: 

                 481     374     243      84
 
AflIII A'CryG_T

Cuts at:      0     249     526    1182 
   Size:        249     277     656

  Fragments arranged by size: 

                 656     277     249
 
AhdI GACnn_n'nnGTC

Cuts at:      0     445    1182 
   Size:        445     737
 
AleI CACnn'nnGTG

Cuts at:      0     764    1182 
   Size:        764     418
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1171    1182 
   Size:       1171      11
 
ApoI r'AATT_y

Cuts at:      0     821    1182 
   Size:        821     361
 
AvaI C'yCGr_G

Cuts at:      0      46    1182 
   Size:         46    1136
 
BamHI G'GATC_C

Cuts at:      0     681    1182 
   Size:        681     501
 
BanI G'GyrC_C

Cuts at:      0     465     926    1182 
   Size:        465     461     256

  Fragments arranged by size: 

                 465     461     256
 
BanII G_rGCy'C

Cuts at:      0     323     401     799    1182 
   Size:        323      78     398     383

  Fragments arranged by size: 

                 398     383     323      78
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     945    1182 
   Size:        945     237
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     155     189    1182 
   Size:        155      34     993

  Fragments arranged by size: 

                 993     155      34
 
BlpI GC'TnA_GC

Cuts at:      0    1172    1182 
   Size:       1172      10
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     806    1182 
   Size:        806     376
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     202     307     310     400     409     851    1182 
   Size:        202     105       3      90       9     442     331

  Fragments arranged by size: 

                 442     331     202     105      90       9       3
 
Bpu10I CC'TnA_GC

Cuts at:      0     604     798    1182 
   Size:        604     194     384

  Fragments arranged by size: 

                 604     384     194
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     178     808    1182 
   Size:        178     630     374

  Fragments arranged by size: 

                 630     374     178
 
BseYI C'CCAG_C

Cuts at:      0     329     503    1182 
   Size:        329     174     679

  Fragments arranged by size: 

                 679     329     174
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1038    1047    1182 
   Size:       1038       9     135

  Fragments arranged by size: 

                1038     135       9
 
BsiHKAI G_wGCw'C

Cuts at:      0     259     323     401     799    1182 
   Size:        259      64      78     398     383

  Fragments arranged by size: 

                 398     383     259      78      64
 
Bsp1286I G_dGCh'C

Cuts at:      0     259     323     401     799    1182 
   Size:        259      64      78     398     383

  Fragments arranged by size: 

                 398     383     259      78      64
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1025    1182 
   Size:       1025     157
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     556    1182 
   Size:        556     626
 
BstYI r'GATC_y

Cuts at:      0     276     384     681    1157    1182 
   Size:        276     108     297     476      25

  Fragments arranged by size: 

                 476     297     276     108      25
 
Bsu36I CC'TnA_GG

Cuts at:      0     509    1182 
   Size:        509     673
 
BtsI GCAGTG_nn'

Cuts at:      0     736     930    1131    1182 
   Size:        736     194     201      51

  Fragments arranged by size: 

                 736     201     194      51
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      59      94     924     959    1182 
   Size:         59      35     830      35     223

  Fragments arranged by size: 

                 830     223      59      35      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    1167    1182 
   Size:       1167      15
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     498    1182 
   Size:        498     684
 
EarI CTCTTCn'nnn_

Cuts at:      0     356     775    1182 
   Size:        356     419     407

  Fragments arranged by size: 

                 419     407     356
 
EcoICRI GAG'CTC

Cuts at:      0     321     399     797    1182 
   Size:        321      78     398     385

  Fragments arranged by size: 

                 398     385     321      78
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     202     307     310     400     409     481     851     855 
   Size:        202     105       3      90       9      72     370       4

Cuts at:    855     939    1182 
   Size:         84     243

  Fragments arranged by size: 

                 370     243     202     105      90      84      72       9
                   4       3
 
EcoRI G'AATT_C

Cuts at:      0     821    1182 
   Size:        821     361
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      10      42     261     293     431     453     463     485 
   Size:         10      32     219      32     138      22      10      22

Cuts at:    485     786     818     954     986    1026    1058    1182 
   Size:        301      32     136      32      40      32     124

  Fragments arranged by size: 

                 301     219     138     136     124      40      32      32
                  32      32      32      22      22      10      10
 
HindIII A'AGCT_T

Cuts at:      0    1119    1182 
   Size:       1119      63
 
KpnI G_GTAC'C

Cuts at:      0     930    1182 
   Size:        930     252
 
MslI CAynn'nnrTG

Cuts at:      0     764    1182 
   Size:        764     418
 
MspA1I CmG'CkG

Cuts at:      0     171    1182 
   Size:        171    1011
 
NspI r_CATG'y

Cuts at:      0     253     530    1182 
   Size:        253     277     652

  Fragments arranged by size: 

                 652     277     253
 
PasI CC'CwG_GG

Cuts at:      0      30    1182 
   Size:         30    1152
 
PciI A'CATG_T

Cuts at:      0     249     526    1182 
   Size:        249     277     656

  Fragments arranged by size: 

                 656     277     249
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     355    1167    1182 
   Size:        355     812      15

  Fragments arranged by size: 

                 812     355      15
 
PfoI T'CCnGG_A

Cuts at:      0     218    1182 
   Size:        218     964
 
PvuII CAG'CTG

Cuts at:      0     171    1182 
   Size:        171    1011
 
SacI G_AGCT'C

Cuts at:      0     323     401     799    1182 
   Size:        323      78     398     383

  Fragments arranged by size: 

                 398     383     323      78
 
SapI GCTCTTCn'nnn_

Cuts at:      0     775    1182 
   Size:        775     407
 
SfcI C'TryA_G

Cuts at:      0    1038    1182 
   Size:       1038     144
 
StyI C'CwwG_G

Cuts at:      0     196     314    1182 
   Size:        196     118     868

  Fragments arranged by size: 

                 868     196     118
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      22     321    1182 
   Size:         22     299     861

  Fragments arranged by size: 

                 861     299      22
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     187    1182 
   Size:        187     995
 
XmnI GAAnn'nnTTC

Cuts at:      0      62     868     952    1182 
   Size:         62     806      84     230

  Fragments arranged by size: 

                 806     230      84      62
 

 Enzymes that do cut:

   Acc65I     AcuI   AflIII     AhdI     AleI    AlwNI     ApoI     AvaI
    BamHI     BanI    BanII     BbsI     BcgI     BlpI     BplI     BpmI
   Bpu10I     BsaI    BseYI     BsgI  BsiHKAI Bsp1286I   BstAPI    BstXI
    BstYI   Bsu36I     BtsI    CspCI   DraIII     DrdI     EarI  EcoICRI
  Eco57MI    EcoRI    Hin4I  HindIII     KpnI     MslI   MspA1I     NspI
     PasI     PciI    PflMI     PfoI    PvuII     SacI     SapI     SfcI
     StyI    TaqII     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII     AgeI     AlfI
     AloI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI
     BbeI    BbvCI    BciVI     BclI    BfrBI     BglI    BglII Bme1580I
    BmgBI     BmrI     BmtI    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI
   BstEII  BstZ17I     BtgI    BtgZI     ClaI     DraI     EaeI     EagI
     EciI    EcoNI EcoO109I    EcoRV     FalI     FseI     FspI    FspAI
    HaeII   HincII     HpaI     KasI     MfeI     MluI     MmeI     MscI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII    SacII     SalI
    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com