Restriction map for pF1KB6419
##################################
# NO CUT ENZYMES (  169 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AviII       AvrII       
BalI        BamHI       BanII       BbeI        BbrPI       
BbvCI       BcgI        BciVI       BclI        BcnI        
BfrI        BglI        BlnI        BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BsgI        
BsiEI       BsiWI       BsmBI       BsmFI       Bsp1407I    
BspDI       BspEI       BspHI       BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssKI       
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstNI       BstPI       BstUI       BstZ17I     Bsu36I      
CfoI        Cfr10I      ClaI        CpoI        DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRII      EcoRV       
EcoT14I     FauI        FbaI        FseI        FspI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HpaI        HpaII       KasI        
KpnI        Ksp632I     KspI        MamI        MfeI        
MluI        MluNI       MroI        MscI        MspA1I      
MspI        MunI        MvaI        MvnI        NaeI        
NarI        NciI        NcoI        NgoMIV      NheI        
NotI        NruI        NspV        PacI        PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        ScrFI       SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        Tth111I     
Van91I      XbaI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AciI           77  GCGG                 CCGC(-3/-1)
AflIII        774  ACATGT               A^C[GA][CT]GT
AlwI          193  GATCC                GGATC(4/5)
AlwNI         745  CAGCCACTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp700I       259  GAAGATTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         884  GAGCAC               G[AT]GC[AT]^C
AvaI          422  CTCGAG               C^[CT]CG[GA]G
BanI          705  GGCACC               G^G[CT][GA]CC
BbsI          800  GAAGAC               GAAGAC(2/6)
BglII         610  AGATCT               A^GATCT
BlpI          595  GCTCAGC              GC^T[ACGT]AGC
BmyI          884  GAGCAC               G[AGT]GC[ACT]^C
Bpu1102I      595  GCTCAGC              GC^T[ACGT]AGC
BpuAI         800  GAAGAC               GAAGAC(2/6)
BsaJI          19  CCTCGG               C^C[ACGT][ACGT]GG
BseRI         122  GAGGAG               GAGGAG(10/8)
BsiHKAI       884  GAGCAC               G[AT]GC[AT]^C
BsmAI         760  GAGAC                GTCTC(1/5)
BsmI          898  GCATTC               GAATGC(1/-1)
BsoBI         422  CTCGAG               C^[CT]CG[GA]G
Bsp1286I      884  GAGCAC               G[AGT]GC[ACT]^C
BspLU11I      774  ACATGT               A^CATGT
BstF5I        894  GGATG                GGATG(2/0)
BstXI         329  CCAACAATGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I         540  GCAGGC               GC[ACGT]^[ACGT]GC
CelII         595  GCTCAGC              GC^T[ACGT]AGC
DraI          813  TTTAAA               TTT^AAA
DraII         842  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoO109I      842  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoT22I       896  ATGCAT               ATGCA^T
FokI          894  GGATG                GGATG(9/13)
MaeII         664  ACGT                 A^CGT
MslI          678  CATCAAAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeI          764  CATATG               CA^TATG
NsiI          896  ATGCAT               ATGCA^T
NspI          774  ACATGT               [GA]CATG^[CT]
PaeR7I        422  CTCGAG               C^TCGAG
PleI          420  GACTC                GAGTC(4/5)
PpuMI         842  AGGACCT              [GA]G^G[AT]CC[CT]
TaqI          423  TCGA                 T^CGA
TfiI          519  GATTC                G^A[AT]TC
TthHB8I       423  TCGA                 T^CGA
XcmI          353  CCAAAAAGAAAATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          422  CTCGAG               C^TCGAG
XmnI          259  GAAGATTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AfaI          149  GTAC                 GT^AC
              178  GTAC                
AvaII         769  GGACC                G^G[AT]CC
              843  GGACC               
BfaI          269  CTAG                 C^TAG
              796  CTAG                
BpmI          559  CTGGAG               CTGGAG(16/14)
              851  CTGGAG              
BsiYI          35  CCAAATATCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              139  CCTGAGTGTGG         
BslI           35  CCAAATATCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              139  CCTGAGTGTGG         
BstYI         192  AGATCC               [GA]^GATC[CT]
              610  AGATCT              
Cfr13I        769  GGACC                G^G[ACGT]CC
              843  GGACC               
HincII        202  GTTGAC               GT[CT]^[GA]AC
              689  GTCAAC              
HindII        202  GTTGAC               GT[CT]^[GA]AC
              689  GTCAAC              
HindIII       446  AAGCTT               A^AGCTT
              718  AAGCTT              
HinfI         420  GACTC                G^A[ACGT]TC
              519  GATTC               
HphI           66  GGTGA                GGTGA(8/7)
              835  TCACC               
MaeI          269  CTAG                 C^TAG
              796  CTAG                
MaeIII        661  GTGAC                ^GT[ACGT]AC
              878  GTCAC               
MflI          192  AGATCC               [GA]^GATC[CT]
              610  AGATCT              
MseI          412  TTAA                 T^TAA
              814  TTAA                
MwoI          786  GCTTGAGATGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              909  GCTTTTCTGGC         
NlaIII        775  CATG                 CATG^
              820  CATG                
NlaIV          44  GGAACC               GG[ACGT]^[ACGT]CC
              705  GGCACC              
PstI          511  CTGCAG               CTGCA^G
              751  CTGCAG              
RsaI          149  GTAC                 GT^AC
              178  GTAC                
Sau96I        769  GGACC                G^G[ACGT]CC
              843  GGACC               
Tru9I         412  TTAA                 T^TAA
              814  TTAA                
Tsp45I        661  GTGAC                ^GT[GC]AC
              878  GTCAC               
XhoII         192  AGATCC               [GA]^GATC[CT]
              610  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   18 enzymes) #
#########################################

AluI        BbvI        BsrI        DdeI        DpnII       
Fnu4HI      ItaI        MboI        MboII       MnlI        
NdeII       Sau3AI      SfaNI       SfcI        SmlI        
TseI        Tsp509I     TspRI       
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