Restriction map for pF1KB6419
 (Linear) (Six-base) MAPSORT of: KIBB6419.seq  Check: 4778  from: 1  to: 927



KIBB6419.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:47  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     156     927 
   Size:        156     771
 
AflIII A'CryG_T

Cuts at:      0     774     927 
   Size:        774     153
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     761     793     927 
   Size:        761      32     134

  Fragments arranged by size: 

                 761     134      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     750     927 
   Size:        750     177
 
AvaI C'yCGr_G

Cuts at:      0     422     927 
   Size:        422     505
 
BanI G'GyrC_C

Cuts at:      0     705     927 
   Size:        705     222
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     807     927 
   Size:        807     120
 
BfrBI ATG'CAT

Cuts at:      0     898     927 
   Size:        898      29
 
BglII A'GATC_T

Cuts at:      0     610     927 
   Size:        610     317
 
BlpI GC'TnA_GC

Cuts at:      0     596     927 
   Size:        596     331
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     917     927 
   Size:        917      10
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     580     872     927 
   Size:        580     292      55

  Fragments arranged by size: 

                 580     292      55
 
Bpu10I CC'TnA_GC

Cuts at:      0     782     927 
   Size:        782     145
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     655     808     927 
   Size:        655     153     119

  Fragments arranged by size: 

                 655     153     119
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     137     927 
   Size:        137     790
 
BsiHKAI G_wGCw'C

Cuts at:      0     888     927 
   Size:        888      39
 
BsmI GAATG_Cn'

Cuts at:      0     898     927 
   Size:        898      29
 
Bsp1286I G_dGCh'C

Cuts at:      0     888     927 
   Size:        888      39
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     336     927 
   Size:        336     591
 
BstYI r'GATC_y

Cuts at:      0     192     610     927 
   Size:        192     418     317

  Fragments arranged by size: 

                 418     317     192
 
BtsI GCAGTG_nn'

Cuts at:      0     748     927 
   Size:        748     179
 
DraI TTT'AAA

Cuts at:      0     815     927 
   Size:        815     112
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     156     580     872     927 
   Size:        156     424     292      55

  Fragments arranged by size: 

                 424     292     156      55
 
EcoO109I rG'GnC_Cy

Cuts at:      0     843     927 
   Size:        843      84
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     186     927 
   Size:        186     741
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      71     103     555     587     927 
   Size:         71      32     452      32     340

  Fragments arranged by size: 

                 452     340      71      32      32
 
HincII GTy'rAC

Cuts at:      0     204     691     927 
   Size:        204     487     236

  Fragments arranged by size: 

                 487     236     204
 
HindIII A'AGCT_T

Cuts at:      0     446     718     927 
   Size:        446     272     209

  Fragments arranged by size: 

                 446     272     209
 
MslI CAynn'nnrTG

Cuts at:      0     682     927 
   Size:        682     245
 
NdeI CA'TA_TG

Cuts at:      0     765     927 
   Size:        765     162
 
NsiI A_TGCA'T

Cuts at:      0     900     927 
   Size:        900      27
 
NspI r_CATG'y

Cuts at:      0     778     927 
   Size:        778     149
 
PciI A'CATG_T

Cuts at:      0     774     927 
   Size:        774     153
 
PpuMI rG'GwC_Cy

Cuts at:      0     843     927 
   Size:        843      84
 
PstI C_TGCA'G

Cuts at:      0     515     755     927 
   Size:        515     240     172

  Fragments arranged by size: 

                 515     240     172
 
SfcI C'TryA_G

Cuts at:      0     511     751     889     927 
   Size:        511     240     138      38

  Fragments arranged by size: 

                 511     240     138      38
 
SmlI C'TyrA_G

Cuts at:      0     422     634     787     927 
   Size:        422     212     153     140

  Fragments arranged by size: 

                 422     212     153     140
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     360     927 
   Size:        360     567
 
XhoI C'TCGA_G

Cuts at:      0     422     927 
   Size:        422     505
 
XmnI GAAnn'nnTTC

Cuts at:      0     263     927 
   Size:        263     664
 

 Enzymes that do cut:

     AcuI   AflIII     AloI    AlwNI     AvaI     BanI     BbsI    BfrBI
    BglII     BlpI     BmrI     BpmI   Bpu10I    BpuEI    BseRI  BsiHKAI
     BsmI Bsp1286I    BstXI    BstYI     BtsI     DraI  Eco57MI EcoO109I
     FalI    Hin4I   HincII  HindIII     MslI     NdeI     NsiI     NspI
     PciI    PpuMI     PstI     SfcI     SmlI     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI    BanII     BbeI    BbvCI     BcgI
    BciVI     BclI     BglI Bme1580I    BmgBI     BmtI     BplI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI
    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I
     BtgI    BtgZI     ClaI    CspCI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FseI     FspI
    FspAI    HaeII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI     NotI
     NruI     PacI     PasI    PflMI     PfoI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XmaI     ZraI
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