################################## # NO CUT ENZYMES ( 197 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BbvI BcgI BciVI BclI BcnI BfrI BglI BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsiYI BslI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoT22I FauI FbaI Fnu4HI FseI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII HphI ItaI KasI KpnI Ksp632I KspI MaeIII MamI MluI MluNI MroI MscI MspA1I MspI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NsiI NspI PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TseI Tsp45I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AfaI 241 GTAC GT^AC AluI 336 AGCT AG^CT AlwI 367 GGATC GGATC(4/5) AspI 230 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 276 GGACC G^G[AT]CC BfaI 338 CTAG C^TAG BglII 174 AGATCT A^GATCT BseRI 53 GAGGAG GAGGAG(10/8) BsgI 59 GTGCAG GTGCAG(16/14) BsmAI 87 GAGAC GTCTC(1/5) BsrDI 214 GCAATG GCAATG(2/0) BssKI 293 CCAGG ^CC[ACGT]GG BstBI 269 TTCGAA TT^CGAA BstF5I 132 GGATG GGATG(2/0) BstNI 293 CCAGG CC^[AT]GG Cfr13I 276 GGACC G^G[ACGT]CC DdeI 152 CTGAG C^T[ACGT]AG DraII 275 AGGACCC [GA]G^G[ACGT]CC[CT] EcoO109I 275 AGGACCC [GA]G^G[ACGT]CC[CT] EcoRII 293 CCAGG ^CC[AT]GG EcoRV 253 GATATC GAT^ATC EcoT14I 280 CCAAGG C^C[AT][AT]GG FokI 132 GGATG GGATG(9/13) MaeI 338 CTAG C^TAG MaeII 25 ACGT A^CGT MfeI 221 CAATTG C^AATTG MseI 299 TTAA T^TAA MslI 195 CACTGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 221 CAATTG C^AATTG MvaI 293 CCAGG CC^[AT]GG MwoI 337 GCTAGAAAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NlaIV 276 GGACCC GG[ACGT]^[ACGT]CC NspV 269 TTCGAA TT^CGAA PflFI 230 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 275 AGGACCC [GA]G^G[AT]CC[CT] RsaI 241 GTAC GT^AC Sau96I 276 GGACC G^G[ACGT]CC ScrFI 293 CCAGG CC^[ACGT]GG SfuI 269 TTCGAA TT^CGAA StyI 280 CCAAGG C^C[AT][AT]GG Tru9I 299 TTAA T^TAA Tth111I 230 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### BsaJI 280 CCAAGG C^C[ACGT][ACGT]GG 293 CCAGGG BstYI 174 AGATCT [GA]^GATC[CT] 367 GGATCT DpnII 175 GATC ^GATC 368 GATC MboI 175 GATC ^GATC 368 GATC MflI 174 AGATCT [GA]^GATC[CT] 367 GGATCT NdeII 175 GATC ^GATC 368 GATC PleI 204 GACTC GAGTC(4/5) 154 GAGTC Sau3AI 175 GATC ^GATC 368 GATC TaqI 237 TCGA T^CGA 270 TCGA TfiI 141 GAATC G^A[AT]TC 267 GATTC TspRI 158 CAGTG CA[GC]TG[ACGT][ACGT]^ 195 CACTG TthHB8I 237 TCGA T^CGA 270 TCGA XhoII 174 AGATCT [GA]^GATC[CT] 367 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 6 enzymes) # ######################################### BbsI BpuAI HinfI MboII MnlI Tsp509I