Restriction map for
pF1KB8622
(Circular) (Six-base) MAPSORT of: pF1KB8622.seq Check: 3353 from: 1 to: 5630
pF1KB8622 5630 bp
With 182 enzymes: SgfI *
March 2, 2010 14:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1121 1121
Size: 5630
AatII G_ACGT'C
Cuts at: 530 1493 530
Size: 963 4667
AccI GT'mk_AC
Cuts at: 530 2668 530
Size: 2138 3492
Acc65I G'GTAC_C
Cuts at: 2646 2646
Size: 5630
AclI AA'CG_TT
Cuts at: 2391 5470 2391
Size: 3079 2551
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 695 996 1060 2353 3455 3887 4634 4902 695
Size: 301 64 1293 1102 432 747 268 1423
Fragments arranged by size:
1423 1293 1102 747 432 301 268 64
AfeI AGC'GCT
Cuts at: 2194 2194
Size: 5630
AflIII A'CryG_T
Cuts at: 869 1664 1733 2982 4086 5063 869
Size: 795 69 1249 1104 977 1436
Fragments arranged by size:
1436 1249 1104 977 795 69
AgeI A'CCGG_T
Cuts at: 4018 4018
Size: 5630
AhdI GACnn_n'nnGTC
Cuts at: 1950 1950
Size: 5630
AleI CACnn'nnGTG
Cuts at: 2452 2452
Size: 5630
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 172 172
Size: 5630
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1316 1348 1316
Size: 32 5598
AlwNI CAG_nnn'CTG
Cuts at: 216 246 315 993 2935 4502 216
Size: 30 69 678 1942 1567 1344
Fragments arranged by size:
1942 1567 1344 678 69 30
ApaLI G'TGCA_C
Cuts at: 1032 4400 1032
Size: 3368 2262
ApoI r'AATT_y
Cuts at: 361 1057 1255 1275 1297 2284 2634 4983 5239
Size: 696 198 20 22 987 350 2349 256
Cuts at: 5239 361
Size: 752
Fragments arranged by size:
2349 987 752 696 350 256 198 22
20
AseI AT'TA_AT
Cuts at: 20 20
Size: 5630
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5630
AvaI C'yCGr_G
Cuts at: 1888 2066 2384 2477 2650 1888
Size: 178 318 93 173 4868
Fragments arranged by size:
4868 318 178 173 93
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1722 1755 2353 2386 1722
Size: 33 598 33 4966
Fragments arranged by size:
4966 598 33 33
BamHI G'GATC_C
Cuts at: 2261 2655 2261
Size: 394 5236
BanI G'GyrC_C
Cuts at: 1714 2646 3060 3308 3343 1714
Size: 932 414 248 35 4001
Fragments arranged by size:
4001 932 414 248 35
BanII G_rGCy'C
Cuts at: 772 793 1430 2644 3674 772
Size: 21 637 1214 1030 2728
Fragments arranged by size:
2728 1214 1030 637 21
BbeI G_GCGC'C
Cuts at: 3064 3312 3064
Size: 248 5382
BbsI GAAGACnn'nnnn_
Cuts at: 189 804 900 1311 189
Size: 615 96 411 4508
Fragments arranged by size:
4508 615 411 96
BbvCI CC'TCA_GC
Cuts at: 1936 1936
Size: 5630
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2065 2099 2065
Size: 34 5596
BciVI GTATCCnnnnn_n'
Cuts at: 1723 3528 4289 5630 1723
Size: 1805 761 1341 1723
Fragments arranged by size:
1805 1723 1341 761
BclI T'GATC_A
Cuts at: 833 2165 3150 833
Size: 1332 985 3313
Fragments arranged by size:
3313 1332 985
BglI GCCn_nnn'nGGC
Cuts at: 1158 2404 2537 1158
Size: 1246 133 4251
Fragments arranged by size:
4251 1246 133
BglII A'GATC_T
Cuts at: 1812 3145 5182 1812
Size: 1333 2037 2260
Fragments arranged by size:
2260 2037 1333
BlpI GC'TnA_GC
Cuts at: 787 2189 2742 787
Size: 1402 553 3675
Fragments arranged by size:
3675 1402 553
Bme1580I G_kGCm'C
Cuts at: 1036 1717 1761 3255 3348 4404 1036
Size: 681 44 1494 93 1056 2262
Fragments arranged by size:
2262 1494 1056 681 93 44
BmgBI CAC'GTC
Cuts at: 870 1923 2514 870
Size: 1053 591 3986
Fragments arranged by size:
3986 1053 591
BmrI ACTGGGnnnn_n'
Cuts at: 303 2416 2842 2923 3258 5402 303
Size: 2113 426 81 335 2144 531
Fragments arranged by size:
2144 2113 531 426 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3997 3997
Size: 5630
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 562 724 763 844 1312 2320 2385 2394 562
Size: 162 39 81 468 1008 65 9 3798
Fragments arranged by size:
3798 1008 468 162 81 65 39 9
Bpu10I CC'TnA_GC
Cuts at: 1936 1936
Size: 5630
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 262 1260 1414 1948 2122 2350 2806 3685 4177
Size: 998 154 534 174 228 456 879 492
Cuts at: 4177 4475 262
Size: 298 1417
Fragments arranged by size:
1417 998 879 534 492 456 298 228
174 154
BsaI GGTCTCn'nnnn_
Cuts at: 499 709 1228 2537 2560 5334 499
Size: 210 519 1309 23 2774 795
Fragments arranged by size:
2774 1309 795 519 210 23
BsaAI yAC'GTr
Cuts at: 2604 2983 2604
Size: 379 5251
BsaBI GATnn'nnATC
Cuts at: 6 1207 2266 3144 5181 6
Size: 1201 1059 878 2037 455
Fragments arranged by size:
2037 1201 1059 878 455
BsaHI Gr'CG_yC
Cuts at: 527 1490 1751 1820 3061 3309 4011 5507 527
Size: 963 261 69 1241 248 702 1496 650
Fragments arranged by size:
1496 1241 963 702 650 261 248 69
BsaWI w'CCGG_w
Cuts at: 1334 1825 2819 3340 4018 4292 4439 4850 1334
Size: 491 994 521 678 274 147 411 2114
Fragments arranged by size:
2114 994 678 521 491 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 241 271 241
Size: 30 5600
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 517 559 1042 2626 517
Size: 42 483 1584 3521
Fragments arranged by size:
3521 1584 483 42
BseYI C'CCAG_C
Cuts at: 1878 2005 3055 3813 4390 4886 1878
Size: 127 1050 758 577 496 2622
Fragments arranged by size:
2622 1050 758 577 496 127
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 232 442 667 232
Size: 210 225 5195
Fragments arranged by size:
5195 225 210
BsiEI CG_ry'CG
Cuts at: 104 132 191 298 2059 3172 3218 4426 104
Size: 28 59 107 1761 1113 46 1208 1308
Fragments arranged by size:
1761 1308 1208 1113 107 59 46 28
BsiHKAI G_wGCw'C
Cuts at: 968 1036 1103 1430 2644 3422 3612 4404 968
Size: 68 67 327 1214 778 190 792 2194
Fragments arranged by size:
2194 1214 792 778 327 190 68 67
BsiWI C'GTAC_G
Cuts at: 2600 2600
Size: 5630
Bsp1286I G_dGCh'C
Cuts at: 772 793 968 1036 1103 1430 1717 1761 2644
Size: 21 175 68 67 327 287 44 883
Cuts at: 2644 3255 3348 3422 3612 3674 4404 772
Size: 611 93 74 190 62 730 1998
Fragments arranged by size:
1998 883 730 611 327 287 190 175
93 74 68 67 62 44 21
BspEI T'CCGG_A
Cuts at: 1825 4850 1825
Size: 3025 2605
BspHI T'CATG_A
Cuts at: 4806 4806
Size: 5630
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1082 1121 2092 2141 2680 2894 3196 3577 1082
Size: 39 971 49 539 214 302 381 3135
Fragments arranged by size:
3135 971 539 381 302 214 49 39
BsrBI CCG'CTC
Cuts at: 50 1831 2175 3922 3976 5460 5625 50
Size: 1781 344 1747 54 1484 165 55
Fragments arranged by size:
1781 1747 1484 344 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3542 3542
Size: 5630
BsrFI r'CCGG_y
Cuts at: 2054 2414 3628 3809 4018 2054
Size: 360 1214 181 209 3666
Fragments arranged by size:
3666 1214 360 209 181
BsrGI T'GTAC_A
Cuts at: 1205 1661 1205
Size: 456 5174
BssSI C'ACGA_G
Cuts at: 3901 4259 3901
Size: 358 5272
BstAPI GCAn_nnn'nTGC
Cuts at: 216 1074 1098 1694 3007 216
Size: 858 24 596 1313 2839
Fragments arranged by size:
2839 1313 858 596 24
BstBI TT'CG_AA
Cuts at: 3991 3991
Size: 5630
BstXI CCAn_nnnn'nTGG
Cuts at: 663 1851 663
Size: 1188 4442
BstYI r'GATC_y
Cuts at: 1 707 1812 2261 2522 2655 3122 3145 3480
Size: 706 1105 449 261 133 467 23 335
Cuts at: 3480 3726 4738 4824 4836 5182 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1105 1012 706 467 449 449 346 335
261 246 133 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 856 1708 856
Size: 852 4778
BtgI C'CryG_G
Cuts at: 107 191 437 1869 1957 107
Size: 84 246 1432 88 3780
Fragments arranged by size:
3780 1432 246 88 84
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 450 1216 3580 3761 3924 450
Size: 766 2364 181 163 2156
Fragments arranged by size:
2364 2156 766 181 163
BtsI GCAGTG_nn'
Cuts at: 121 2081 2873 121
Size: 1960 792 2878
Fragments arranged by size:
2878 1960 792
ClaI AT'CG_AT
Cuts at: 143 143
Size: 5630
DraI TTT'AAA
Cuts at: 2629 2629
Size: 5630
DraIII CAC_nnn'GTG
Cuts at: 174 174
Size: 5630
DrdI GACnn_nn'nnGTC
Cuts at: 526 1390 3336 4194 526
Size: 864 1946 858 1962
Fragments arranged by size:
1962 1946 864 858
EaeI y'GGCC_r
Cuts at: 129 188 295 551 899 995 1604 1853 2056
Size: 59 107 256 348 96 609 249 203
Cuts at: 2056 2456 3215 3389 3780 3807 5152 129
Size: 400 759 174 391 27 1345 607
Fragments arranged by size:
1345 759 609 607 400 391 348 256
249 203 174 107 96 59 27
EagI C'GGCC_G
Cuts at: 129 188 295 2056 3215 129
Size: 59 107 1761 1159 2544
Fragments arranged by size:
2544 1761 1159 107 59
EarI CTCTTCn'nnn_
Cuts at: 409 1570 2603 3653 3863 409
Size: 1161 1033 1050 210 2176
Fragments arranged by size:
2176 1161 1050 1033 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 811 2555 3838 4148 4294 5440 811
Size: 1744 1283 310 146 1146 1001
Fragments arranged by size:
1744 1283 1146 1001 310 146
EcoICRI GAG'CTC
Cuts at: 1428 2642 1428
Size: 1214 4416
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 562 695 724 763 844 996 1060 1312 2320
Size: 133 29 39 81 152 64 252 1008
Cuts at: 2320 2353 2385 2394 3455 3887 4634 4902 562
Size: 33 32 9 1061 432 747 268 1290
Fragments arranged by size:
1290 1061 1008 747 432 268 252 152
133 81 64 39 33 32 29 9
EcoNI CCTnn'n_nnAGG
Cuts at: 208 571 835 208
Size: 363 264 5003
Fragments arranged by size:
5003 363 264
EcoO109I rG'GnC_Cy
Cuts at: 153 228 828 1840 2156 2405 2769 153
Size: 75 600 1012 316 249 364 3014
Fragments arranged by size:
3014 1012 600 364 316 249 75
EcoRI G'AATT_C
Cuts at: 2634 2634
Size: 5630
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1254 1560 2358 3649 1254
Size: 306 798 1291 3235
Fragments arranged by size:
3235 1291 798 306
FspI TGC'GCA
Cuts at: 3411 3411
Size: 5630
HaeII r_GCGC'y
Cuts at: 217 2196 3064 3312 4334 5313 217
Size: 1979 868 248 1022 979 534
Fragments arranged by size:
1979 1022 979 868 534 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1936 1968 3472 3502 3504 3534 1936
Size: 32 1504 30 2 30 4032
Fragments arranged by size:
4032 1504 32 30 30 2
HincII GTy'rAC
Cuts at: 1347 2669 1347
Size: 1322 4308
KasI G'GCGC_C
Cuts at: 3060 3308 3060
Size: 248 5382
KpnI G_GTAC'C
Cuts at: 2650 2650
Size: 5630
MluI A'CGCG_T
Cuts at: 5063 5063
Size: 5630
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4301 4485 4301
Size: 184 5446
MscI TGG'CCA
Cuts at: 901 997 1606 1855 2458 3391 901
Size: 96 609 249 603 933 3140
Fragments arranged by size:
3140 933 609 603 249 96
MslI CAynn'nnrTG
Cuts at: 637 661 868 2239 2452 3619 3746 637
Size: 24 207 1371 213 1167 127 2521
Fragments arranged by size:
2521 1371 1167 213 207 127 24
MspA1I CmG'CkG
Cuts at: 134 243 439 808 2153 2352 2452 2741 3055
Size: 109 196 369 1345 199 100 289 314
Cuts at: 3055 3415 4428 4673 134
Size: 360 1013 245 1091
Fragments arranged by size:
1345 1091 1013 369 360 314 289 245
199 196 109 100
NaeI GCC'GGC
Cuts at: 2056 3811 2056
Size: 1755 3875
NarI GG'CG_CC
Cuts at: 3061 3309 3061
Size: 248 5382
NcoI C'CATG_G
Cuts at: 107 1869 107
Size: 1762 3868
NgoMIV G'CCGG_C
Cuts at: 2054 3809 2054
Size: 1755 3875
NotI GC'GGCC_GC
Cuts at: 129 129
Size: 5630
NspI r_CATG'y
Cuts at: 1737 2683 3183 4090 1737
Size: 946 500 907 3277
Fragments arranged by size:
3277 946 907 500
PciI A'CATG_T
Cuts at: 1733 4086 1733
Size: 2353 3277
PflMI CCAn_nnn'nTGG
Cuts at: 174 993 174
Size: 819 4811
PfoI T'CCnGG_A
Cuts at: 710 1046 2297 710
Size: 336 1251 4043
Fragments arranged by size:
4043 1251 336
PmeI GTTT'AAAC
Cuts at: 2629 2629
Size: 5630
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1435 1467 4769 4801 1435
Size: 32 3302 32 2264
Fragments arranged by size:
3302 2264 32 32
PpuMI rG'GwC_Cy
Cuts at: 153 228 828 1840 2156 153
Size: 75 600 1012 316 3627
Fragments arranged by size:
3627 1012 600 316 75
PstI C_TGCA'G
Cuts at: 955 1812 2677 3362 955
Size: 857 865 685 3223
Fragments arranged by size:
3223 865 857 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5630
PvuII CAG'CTG
Cuts at: 243 808 2352 2452 3055 3415 243
Size: 565 1544 100 603 360 2458
Fragments arranged by size:
2458 1544 603 565 360 100
RsrII CG'GwC_CG
Cuts at: 3825 3825
Size: 5630
SacI G_AGCT'C
Cuts at: 1430 2644 1430
Size: 1214 4416
SacII CC_GC'GG
Cuts at: 440 440
Size: 5630
SalI G'TCGA_C
Cuts at: 2667 2667
Size: 5630
SapI GCTCTTCn'nnn_
Cuts at: 3653 3863 3653
Size: 210 5420
SbfI CC_TGCA'GG
Cuts at: 2677 2677
Size: 5630
ScaI AGT'ACT
Cuts at: 730 949 2314 5147 730
Size: 219 1365 2833 1213
Fragments arranged by size:
2833 1365 1213 219
SexAI A'CCwGG_T
Cuts at: 1836 1836
Size: 5630
SfcI C'TryA_G
Cuts at: 33 951 1268 1416 1808 2526 2673 3358 4351
Size: 918 317 148 392 718 147 685 993
Cuts at: 4351 4542 33
Size: 191 1121
Fragments arranged by size:
1121 993 918 718 685 392 317 191
148 147
SfiI GGCCn_nnn'nGGCC
Cuts at: 2404 2404
Size: 5630
SfoI GGC'GCC
Cuts at: 3062 3310 3062
Size: 248 5382
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5630
SmaI CCC'GGG
Cuts at: 1890 2068 2386 2652 1890
Size: 178 318 266 4868
Fragments arranged by size:
4868 318 266 178
SmlI C'TyrA_G
Cuts at: 277 1239 1429 1927 2137 2365 2785 3700 4192
Size: 962 190 498 210 228 420 915 492
Cuts at: 4192 4454 277
Size: 262 1453
Fragments arranged by size:
1453 962 915 498 492 420 262 228
210 190
SnaBI TAC'GTA
Cuts at: 2604 2604
Size: 5630
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5630
SphI G_CATG'C
Cuts at: 2683 3183 2683
Size: 500 5130
StuI AGG'CCT
Cuts at: 489 489
Size: 5630
StyI C'CwwG_G
Cuts at: 107 902 1869 2764 3118 107
Size: 795 967 895 354 2619
Fragments arranged by size:
2619 967 895 795 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 733 1480 3213 3346 3805 4014 733
Size: 747 1733 133 459 209 2349
Fragments arranged by size:
2349 1733 747 459 209 133
TatI w'GTAC_w
Cuts at: 728 947 1205 1661 2312 5145 728
Size: 219 258 456 651 2833 1213
Fragments arranged by size:
2833 1213 651 456 258 219
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 241 273 1028 1060 5147 5179 241
Size: 32 755 32 4087 32 692
Fragments arranged by size:
4087 755 692 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 226 589 1838 3427 226
Size: 363 1249 1589 2429
Fragments arranged by size:
2429 1589 1249 363
XbaI T'CTAG_A
Cuts at: 2661 2661
Size: 5630
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 171 361 1194 3125 171
Size: 190 833 1931 2676
Fragments arranged by size:
2676 1931 833 190
XmaI C'CCGG_G
Cuts at: 1888 2066 2384 2650 1888
Size: 178 318 266 4868
Fragments arranged by size:
4868 318 266 178
XmnI GAAnn'nnTTC
Cuts at: 929 929
Size: 5630
ZraI GAC'GTC
Cuts at: 528 1491 528
Size: 963 4667
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NotI NspI PciI PflMI PfoI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AflII ApaI AscI AvrII BfrBI BmtI BsmI BsmBI
BssHII BstEII BstZ17I CspCI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NruI NsiI PacI PasI
PmlI PshAI PsiI PspOMI PspXI PsrI SanDI SgrAI
SrfI SspI SwaI XhoI