Restriction map for pF1KB7012
##################################
# NO CUT ENZYMES (   68 enzymes) #
##################################

AatII       AclI        AflII       AgeI        Alw44I      
Aor51HI     ApaLI       AscI        AseI        AsnI        
Asp700I     AspI        AviII       AvrII       BfrI        
BglII       BlnI        BsiEI       BsiWI       BsmI        
BspDI       BspLU11I    BsrBI       BssHII      Bst1107I    
BstBI       BstZ17I     ClaI        DraI        EagI        
EclXI       Eco47III    Eco52I      EcoRV       EcoT22I     
FseI        FspI        HindIII     HpaI        MfeI        
MluI        MunI        NdeI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmeI        PshBI       Psp1406I    PvuI        
SalI        SfiI        SfuI        SgrAI       SnaBI       
SpeI        SspI        StuI        SwaI        Tth111I     
XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AccIII       2108  TCCGGA               T^CCGGA
AflIII       1754  ACACGT               A^C[GA][CT]GT
AhdI         1780  GACAGCGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         2141  CCTCAGG              CC^T[ACGT]AGG
AspEI        1780  GACAGCGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI         2293  GGCGCC               GGCGC^C
BbrPI        1755  CACGTG               CAC^GTG
BbvCI        2422  GCTGAGG              CCTCAGC(-5/-2)
BciVI        2525  GTATCC               GTATCC(6/5)
BclI         1418  TGATCA               T^GATCA
BsaAI        1755  CACGTG               [CT]AC^GT[GA]
BseAI        2108  TCCGGA               T^CCGGA
BsmBI        2222  GAGACG               CGTCTC(1/5)
Bsp1407I      575  TGTACA               T^GTACA
BspEI        2108  TCCGGA               T^CCGGA
BsrGI         575  TGTACA               T^GTACA
BssSI        2410  CTCGTG               CACGAG(-5/-1)
BstEII        768  GGTCACC              G^GT[ACGT]ACC
BstPI         768  GGTCACC              G^GT[ACGT]ACC
Bsu36I       2141  CCTCAGG              CC^T[ACGT]AGG
CpoI         2582  CGGACCG              CG^G[AT]CCG
Eam1105I     1780  GACAGCGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       2141  CCTCAGG              CC^T[ACGT]AGG
EcoNI        2246  CCTTCCGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       768  GGTCACC              G^GT[ACGT]ACC
EcoRI         400  GAATTC               G^AATTC
FbaI         1418  TGATCA               T^GATCA
KasI         2293  GGCGCC               G^GCGCC
MroI         2108  TCCGGA               T^CCGGA
MseI         2789  TTAA                 T^TAA
NaeI         1051  GCCGGC               GCC^GGC
NarI         2293  GGCGCC               GG^CGCC
NgoMIV       1051  GCCGGC               G^CCGGC
NheI         1227  GCTAGC               G^CTAGC
PmaCI        1755  CACGTG               CAC^GTG
PmlI         1755  CACGTG               CAC^GTG
PshAI         538  GACGGGCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII        2582  CGGACCG              CG^G[AT]CCG
SapI         2428  GAAGAGC              GCTCTTC(1/4)
SbfI         1945  CCTGCAGG             CCTGCA^GG
ScaI         2306  AGTACT               AGT^ACT
SexAI         281  ACCTGGT              A^CC[AT]GGT
SfoI         2293  GGCGCC               GGC^GCC
SphI          178  GCATGC               GCATG^C
Sse8387I     1945  CCTGCAGG             CCTGCA^GG
SspBI         575  TGTACA               T^GTACA
Tru9I        2789  TTAA                 T^TAA


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

Acc65I        229  GGTACC               G^GTACC
             1204  GGTACC              
AccI          322  GTCTAC               GT^[AC][GT]AC
             2314  GTCTAC              
AcsI          400  GAATTC               [GA]^AATT[CT]
             2948  AAATTC              
ApoI          400  GAATTC               [GA]^AATT[CT]
             2948  AAATTC              
Asp718I       229  GGTACC               G^GTACC
             1204  GGTACC              
BalI          623  TGGCCA               TGG^CCA
             2931  TGGCCA              
BamHI          88  GGATCC               G^GATCC
             2978  GGATCC              
BcgI         1989  CGAGGGCAATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             2122  GCAGCCCCCTCG        
BsaWI        1679  ACCGGA               [AT]^CCGG[AT]
             2108  TCCGGA              
BspHI         290  TCATGA               T^CATGA
              435  TCATGA              
BsrDI         579  CATTGC               GCAATG(2/0)
             1994  GCAATG              
BstAPI        165  GCAGGTGTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              307  GCACAGGATGC         
DrdI         2193  GACGCCCATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2797  GACCCTGTGGTC        
EaeI          623  TGGCCA               [CT]^GGCC[GA]
             2931  TGGCCA              
EarI           63  CTCTTC               CTCTTC(1/4)
             2428  GAAGAG              
HaeII        1219  AGCGCC               [GA]GCGC^[CT]
             2293  GGCGCC              
HincII       1378  GTCAAC               GT[CT]^[GA]AC
             2185  GTCAAC              
HindII       1378  GTCAAC               GT[CT]^[GA]AC
             2185  GTCAAC              
KpnI          229  GGTACC               GGTAC^C
             1204  GGTACC              
Ksp632I        63  CTCTTC               CTCTTC(1/4)
             2428  GAAGAG              
MaeII        1756  ACGT                 A^CGT
             2489  ACGT                
MluNI         623  TGGCCA               TGG^CCA
             2931  TGGCCA              
MscI          623  TGGCCA               TGG^CCA
             2931  TGGCCA              
NcoI         1610  CCATGG               C^CATGG
             1740  CCATGG              
NspI          178  GCATGC               [GA]CATG^[CT]
             1172  GCATGT              
RcaI          290  TCATGA               T^CATGA
              435  TCATGA              
SacI         2176  GAGCTC               GAGCT^C
             3115  GAGCTC              
SmlI         1545  CTCAAG               C^T[CT][GA]AG
             2769  CTCAAG              
TfiI         1263  GAATC                G^A[AT]TC
             1502  GAATC               
Tsp509I       401  AATT                 ^AATT
             2949  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (  113 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       ApaI        AspHI       AvaI        
AvaII       BanI        BanII       BbsI        BbvI        
BcnI        BfaI        BglI        BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaBI       BsaHI       
BsaI        BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BspMI       BsrFI       BsrI        BssKI       
BstF5I      BstNI       BstUI       BstXI       BstYI       
Cac8I       CelII       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DraIII      DsaI        
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        KspI        MaeI        MaeIII      MamI        
MboI        MboII       MflI        MnlI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PflMI       
PleI        PpuMI       PstI        PvuII       RsaI        
SacII       Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmaI        StyI        TaqI        TseI        
Tsp45I      TspRI       TthHB8I     Van91I      XcmI        
XhoII       XmaCI       XmaI        
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