################################## # NO CUT ENZYMES ( 115 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI AscI AseI AsnI Asp700I Asp718I AspEI AvrII BbrPI BbsI BcgI BciVI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BseAI BsiWI BsmBI BsmFI Bsp1407I BspDI BspEI BspLU11I BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FseI HincII HindII HindIII HpaI KpnI KspI MamI MfeI MluI MroI MseI MunI NdeI NheI NotI NruI NsiI NspI NspV PacI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tru9I Van91I XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AccII 1171 CGCG CG^CG AcsI 163 AAATTC [GA]^AATT[CT] AcyI 663 GGCGCC G[GA]^CG[CT]C Alw44I 1090 GTGCAC G^TGCAC AlwNI 637 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 698 CCTCAGG CC^T[ACGT]AGG Aor51HI 1517 AGCGCT AGC^GCT ApaI 767 GGGCCC GGGCC^C ApaLI 1090 GTGCAC G^TGCAC ApoI 163 AAATTC [GA]^AATT[CT] AspI 277 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 569 CTCGAG C^[CT]CG[GA]G AviII 314 TGCGCA TGC^GCA BamHI 615 GGATCC G^GATCC BbeI 663 GGCGCC GGCGC^C BsaAI 1225 TACGTG [CT]AC^GT[GA] BsaHI 663 GGCGCC G[GA]^CG[CT]C BsaWI 1496 TCCGGT [AT]^CCGG[AT] BsgI 998 CTGCAC GTGCAG(16/14) BsmI 890 GCATTC GAATGC(1/-1) BsoBI 569 CTCGAG C^[CT]CG[GA]G BspHI 1145 TCATGA T^CATGA BsrBI 114 CCGCTC CCGCTC(-3/-3) BsrDI 876 CATTGC GCAATG(2/0) BstUI 1171 CGCG CG^CG BstXI 1310 CCACTGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 615 GGATCC [GA]^GATC[CT] Bsu36I 698 CCTCAGG CC^T[ACGT]AGG DsaI 1184 CCATGG C^C[GA][CT]GG EarI 1400 CTCTTC CTCTTC(1/4) Eco47III 1517 AGCGCT AGC^GCT Eco81I 698 CCTCAGG CC^T[ACGT]AGG FspI 314 TGCGCA TGC^GCA HgaI 287 GCGTC GACGC(5/10) Hin1I 663 GGCGCC G[GA]^CG[CT]C KasI 663 GGCGCC G^GCGCC Ksp632I 1400 CTCTTC CTCTTC(1/4) MflI 615 GGATCC [GA]^GATC[CT] MvnI 1171 CGCG CG^CG NarI 663 GGCGCC GG^CGCC NcoI 1184 CCATGG C^CATGG PaeR7I 569 CTCGAG C^TCGAG PflFI 277 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC PstI 136 CTGCAG CTGCA^G PvuII 719 CAGCTG CAG^CTG RcaI 1145 TCATGA T^CATGA SfcI 136 CTGCAG C^T[GA][CT]AG SfoI 663 GGCGCC GGC^GCC SmlI 569 CTCGAG C^T[CT][GA]AG Tth111I 277 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 1370 CCATCAGGCTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 569 CTCGAG C^TCGAG XhoII 615 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AfaI 337 GTAC GT^AC 678 GTAC BalI 8 TGGCCA TGG^CCA 626 TGGCCA BbvCI 441 GCTGAGG CCTCAGC(-5/-2) 823 GCTGAGG BclI 80 TGATCA T^GATCA 195 TGATCA BsiEI 831 CGACCG CG[GA][CT]^CG 1047 CGACCG BspMI 993 ACCTGC ACCTGC(4/8) 1593 GCAGGT BssSI 847 CACGAG CACGAG(-5/-1) 1529 CTCGTG DraII 766 GGGGCCC [GA]G^G[ACGT]CC[CT] 767 GGGCCCT EaeI 8 TGGCCA [CT]^GGCC[GA] 626 TGGCCA EcoO109I 766 GGGGCCC [GA]G^G[ACGT]CC[CT] 767 GGGCCCT EcoT14I 881 CCAAGG C^C[AT][AT]GG 1184 CCATGG FauI 911 CCCGC CCCGC(4/6) 1543 CCCGC FbaI 80 TGATCA T^GATCA 195 TGATCA HphI 428 TCACC GGTGA(8/7) 896 TCACC MluNI 8 TGGCCA TGG^CCA 626 TGGCCA MscI 8 TGGCCA TGG^CCA 626 TGGCCA MspA1I 719 CAGCTG C[AC]G^C[GT]G 912 CCGCTG NaeI 886 GCCGGC GCC^GGC 1103 GCCGGC NgoMIV 886 GCCGGC G^CCGGC 1103 GCCGGC RsaI 337 GTAC GT^AC 678 GTAC SacI 424 GAGCTC GAGCT^C 1579 GAGCTC SfaNI 180 GATGC GCATC(5/9) 1261 GATGC StyI 881 CCAAGG C^C[AT][AT]GG 1184 CCATGG TfiI 155 GATTC G^A[AT]TC 1032 GATTC Tsp45I 23 GTGAC ^GT[GC]AC 289 GTCAC Tsp509I 164 AATT ^AATT 450 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 64 enzymes) # ######################################### AciI AluI AlwI AspHI AvaII BanI BanII BbvI BcnI BfaI BmyI BpmI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MaeI MaeII MaeIII MboI MboII MnlI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI TaqI TseI TspRI TthHB8I