(Circular) (Six-base) MAPSORT of: pF1KB5387.seq Check: 4 from: 1 to: 4742 pF1KB5387 4742 bp With 182 enzymes: SgfI * April 24, 2009 13:04 .. AccI GT'mk_AC Cuts at: 1780 1780 Size: 4742 Acc65I G'GTAC_C Cuts at: 1758 1758 Size: 4742 AclI AA'CG_TT Cuts at: 4582 4582 Size: 4742 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 2567 2999 3352 4014 2567 Size: 432 353 662 3295 Fragments arranged by size: 3295 662 432 353 AfeI AGC'GCT Cuts at: 1627 1627 Size: 4742 AflIII A'CryG_T Cuts at: 2094 3894 4175 2094 Size: 1800 281 2661 Fragments arranged by size: 2661 1800 281 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1724 1724 Size: 4742 AlwNI CAG_nnn'CTG Cuts at: 750 2047 3485 750 Size: 1297 1438 2007 Fragments arranged by size: 2007 1438 1297 ApaI G_GGCC'C Cuts at: 879 879 Size: 4742 ApaLI G'TGCA_C Cuts at: 1198 3580 1198 Size: 2382 2360 ApoI r'AATT_y Cuts at: 271 1746 4095 4351 271 Size: 1475 2349 256 662 Fragments arranged by size: 2349 1475 662 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4742 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4742 AvaI C'yCGr_G Cuts at: 677 1762 677 Size: 1085 3657 BamHI G'GATC_C Cuts at: 723 1767 723 Size: 1044 3698 BanI G'GyrC_C Cuts at: 309 771 1296 1758 2172 2420 2455 309 Size: 462 525 462 414 248 35 2596 Fragments arranged by size: 2596 525 462 462 414 248 35 BanII G_rGCy'C Cuts at: 259 536 805 879 1691 1756 2786 259 Size: 277 269 74 812 65 1030 2215 Fragments arranged by size: 2215 1030 812 277 269 74 65 BbeI G_GCGC'C Cuts at: 775 2176 2424 775 Size: 1401 248 3093 Fragments arranged by size: 3093 1401 248 BbvCI CC'TCA_GC Cuts at: 550 932 550 Size: 382 4360 BciVI GTATCCnnnnn_n' Cuts at: 2640 3696 4742 2640 Size: 1056 1046 2640 Fragments arranged by size: 2640 1056 1046 BclI T'GATC_A Cuts at: 188 303 2262 188 Size: 115 1959 2668 Fragments arranged by size: 2668 1959 115 BglII A'GATC_T Cuts at: 2257 4294 2257 Size: 2037 2705 BlpI GC'TnA_GC Cuts at: 1854 1854 Size: 4742 Bme1580I G_kGCm'C Cuts at: 199 879 1202 2367 2460 3584 199 Size: 680 323 1165 93 1124 1357 Fragments arranged by size: 1357 1165 1124 680 323 93 BmgBI CAC'GTC Cuts at: 390 1432 390 Size: 1042 3700 BmrI ACTGGGnnnn_n' Cuts at: 437 1221 1650 1954 2035 2370 4514 437 Size: 784 429 304 81 335 2144 665 Fragments arranged by size: 2144 784 665 429 335 304 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3109 3109 Size: 4742 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 243 401 548 1102 243 Size: 158 147 554 3883 Fragments arranged by size: 3883 554 158 147 Bpu10I CC'TnA_GC Cuts at: 225 550 932 225 Size: 325 382 4035 Fragments arranged by size: 4035 382 325 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1918 2797 3511 3809 1918 Size: 879 714 298 2851 Fragments arranged by size: 2851 879 714 298 BsaI GGTCTCn'nnnn_ Cuts at: 142 377 1539 1590 4446 142 Size: 235 1162 51 2856 438 Fragments arranged by size: 2856 1162 438 235 51 BsaAI yAC'GTr Cuts at: 1335 2095 1335 Size: 760 3982 BsaBI GATnn'nnATC Cuts at: 6 2256 4293 6 Size: 2250 2037 455 Fragments arranged by size: 2250 2037 455 BsaHI Gr'CG_yC Cuts at: 772 2173 2421 3123 4619 772 Size: 1401 248 702 1496 895 Fragments arranged by size: 1496 1401 895 702 248 BsaWI w'CCGG_w Cuts at: 1604 1931 2452 3130 3541 3688 3962 1604 Size: 327 521 678 411 147 274 2384 Fragments arranged by size: 2384 678 521 411 327 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 374 404 629 659 374 Size: 30 225 30 4457 Fragments arranged by size: 4457 225 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 559 580 665 795 1120 1699 559 Size: 21 85 130 325 579 3602 Fragments arranged by size: 3602 579 325 130 85 21 BseYI C'CCAG_C Cuts at: 374 1181 2167 2925 3590 3998 374 Size: 807 986 758 665 408 1118 Fragments arranged by size: 1118 986 807 758 665 408 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1091 1091 Size: 4742 BsiEI CG_ry'CG Cuts at: 104 942 1158 2284 2330 3560 104 Size: 838 216 1126 46 1230 1286 Fragments arranged by size: 1286 1230 1126 838 216 46 BsiHKAI G_wGCw'C Cuts at: 536 1202 1691 1756 2534 2724 3584 536 Size: 666 489 65 778 190 860 1694 Fragments arranged by size: 1694 860 778 666 489 190 65 BsmI GAATG_Cn' Cuts at: 998 998 Size: 4742 Bsp1286I G_dGCh'C Cuts at: 199 259 536 805 879 1202 1691 1756 2367 Size: 60 277 269 74 323 489 65 611 Cuts at: 2367 2460 2534 2724 2786 3584 199 Size: 93 74 190 62 798 1357 Fragments arranged by size: 1357 798 611 489 323 277 269 190 93 74 74 65 62 60 BspHI T'CATG_A Cuts at: 1253 3174 1253 Size: 1921 2821 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1110 1692 1792 2006 2308 2689 1110 Size: 582 100 214 302 381 3163 Fragments arranged by size: 3163 582 381 302 214 100 BsrBI CCG'CTC Cuts at: 50 224 3034 3088 4572 4737 50 Size: 174 2810 54 1484 165 55 Fragments arranged by size: 2810 1484 174 165 55 54 BsrDI GCAATG_nn' Cuts at: 983 2654 983 Size: 1671 3071 BsrFI r'CCGG_y Cuts at: 994 1148 1211 2740 2921 994 Size: 154 63 1529 181 2815 Fragments arranged by size: 2815 1529 181 154 63 BssSI C'ACGA_G Cuts at: 955 1637 3013 3721 955 Size: 682 1376 708 1976 Fragments arranged by size: 1976 1376 708 682 BstAPI GCAn_nnn'nTGC Cuts at: 2119 2119 Size: 4742 BstBI TT'CG_AA Cuts at: 3103 3103 Size: 4742 BstXI CCAn_nnnn'nTGG Cuts at: 114 1425 114 Size: 1311 3431 BstYI r'GATC_y Cuts at: 1 723 1767 2234 2257 2592 2838 3144 3156 Size: 722 1044 467 23 335 246 306 12 Cuts at: 3156 3242 4294 1 Size: 86 1052 449 Fragments arranged by size: 1052 1044 722 467 449 335 306 246 86 23 12 Bsu36I CC'TnA_GG Cuts at: 807 807 Size: 4742 BtgI C'CryG_G Cuts at: 107 1292 107 Size: 1185 3557 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2692 2873 3036 2692 Size: 181 163 4398 Fragments arranged by size: 4398 181 163 BtsI GCAGTG_nn' Cuts at: 637 1108 1418 1985 637 Size: 471 310 567 3394 Fragments arranged by size: 3394 567 471 310 DraI TTT'AAA Cuts at: 1741 1741 Size: 4742 DrdI GACnn_nn'nnGTC Cuts at: 2448 3792 2448 Size: 1344 3398 EaeI y'GGCC_r Cuts at: 116 734 2327 2501 2892 2919 4264 116 Size: 618 1593 174 391 27 1345 594 Fragments arranged by size: 1593 1345 618 594 391 174 27 EagI C'GGCC_G Cuts at: 2327 2327 Size: 4742 EarI CTCTTCn'nnn_ Cuts at: 1514 2765 2975 1514 Size: 1251 210 3281 Fragments arranged by size: 3281 1251 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2950 3692 3838 4552 2950 Size: 742 146 714 3140 Fragments arranged by size: 3140 742 714 146 EcoICRI GAG'CTC Cuts at: 534 1689 1754 534 Size: 1155 65 3522 Fragments arranged by size: 3522 1155 65 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 243 401 548 1102 2567 2999 3352 4014 243 Size: 158 147 554 1465 432 353 662 971 Fragments arranged by size: 1465 971 662 554 432 353 158 147 EcoO109I rG'GnC_Cy Cuts at: 875 876 1881 875 Size: 1 1005 3736 Fragments arranged by size: 3736 1005 1 EcoRI G'AATT_C Cuts at: 1746 1746 Size: 4742 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2761 2761 Size: 4742 FspI TGC'GCA Cuts at: 424 2523 424 Size: 2099 2643 HaeII r_GCGC'y Cuts at: 775 1062 1629 2176 2424 3654 4425 775 Size: 287 567 547 248 1230 771 1092 Fragments arranged by size: 1230 1092 771 567 547 287 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 153 185 374 406 502 534 708 740 2584 Size: 32 189 32 96 32 174 32 1844 Cuts at: 2584 2614 2616 2646 153 Size: 30 2 30 2249 Fragments arranged by size: 2249 1844 189 174 96 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 1781 1781 Size: 4742 KasI G'GCGC_C Cuts at: 771 2172 2420 771 Size: 1401 248 3093 Fragments arranged by size: 3093 1401 248 KpnI G_GTAC'C Cuts at: 1762 1762 Size: 4742 MluI A'CGCG_T Cuts at: 4175 4175 Size: 4742 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 459 3501 3685 459 Size: 3042 184 1516 Fragments arranged by size: 3042 1516 184 MscI TGG'CCA Cuts at: 118 736 2503 118 Size: 618 1767 2357 Fragments arranged by size: 2357 1767 618 MslI CAynn'nnrTG Cuts at: 112 178 623 1270 2731 2858 112 Size: 66 445 647 1461 127 1996 Fragments arranged by size: 1996 1461 647 445 127 66 MspA1I CmG'CkG Cuts at: 829 1022 1853 2167 2527 3311 3556 829 Size: 193 831 314 360 784 245 2015 Fragments arranged by size: 2015 831 784 360 314 245 193 NaeI GCC'GGC Cuts at: 996 1213 2923 996 Size: 217 1710 2815 Fragments arranged by size: 2815 1710 217 NarI GG'CG_CC Cuts at: 772 2173 2421 772 Size: 1401 248 3093 Fragments arranged by size: 3093 1401 248 NcoI C'CATG_G Cuts at: 107 1292 107 Size: 1185 3557 NgoMIV G'CCGG_C Cuts at: 994 1211 2921 994 Size: 217 1710 2815 Fragments arranged by size: 2815 1710 217 NspI r_CATG'y Cuts at: 1795 2295 3898 1795 Size: 500 1603 2639 Fragments arranged by size: 2639 1603 500 PciI A'CATG_T Cuts at: 3894 3894 Size: 4742 PfoI T'CCnGG_A Cuts at: 275 1714 275 Size: 1439 3303 PmeI GTTT'AAAC Cuts at: 1741 1741 Size: 4742 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3188 3220 3188 Size: 32 4710 PspOMI G'GGCC_C Cuts at: 875 875 Size: 4742 PspXI vC'TCGA_Gb Cuts at: 677 677 Size: 4742 PstI C_TGCA'G Cuts at: 248 1789 2474 248 Size: 1541 685 2516 Fragments arranged by size: 2516 1541 685 PvuI CG_AT'CG Cuts at: 104 104 Size: 4742 PvuII CAG'CTG Cuts at: 829 2167 2527 829 Size: 1338 360 3044 Fragments arranged by size: 3044 1338 360 RsrII CG'GwC_CG Cuts at: 2937 2937 Size: 4742 SacI G_AGCT'C Cuts at: 536 1691 1756 536 Size: 1155 65 3522 Fragments arranged by size: 3522 1155 65 SalI G'TCGA_C Cuts at: 1779 1779 Size: 4742 SapI GCTCTTCn'nnn_ Cuts at: 2765 2975 2765 Size: 210 4532 SbfI CC_TGCA'GG Cuts at: 1789 1789 Size: 4742 ScaI AGT'ACT Cuts at: 4259 4259 Size: 4742 SfcI C'TryA_G Cuts at: 33 244 1785 2470 3438 3629 33 Size: 211 1541 685 968 191 1146 Fragments arranged by size: 1541 1146 968 685 211 191 SfoI GGC'GCC Cuts at: 773 2174 2422 773 Size: 1401 248 3093 Fragments arranged by size: 3093 1401 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4742 SmaI CCC'GGG Cuts at: 1764 1764 Size: 4742 SmlI C'TyrA_G Cuts at: 677 1897 2812 3526 3788 677 Size: 1220 915 714 262 1631 Fragments arranged by size: 1631 1220 915 714 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4742 SphI G_CATG'C Cuts at: 1795 2295 1795 Size: 500 4242 StyI C'CwwG_G Cuts at: 107 989 1292 1876 2230 107 Size: 882 303 584 354 2619 Fragments arranged by size: 2619 882 584 354 303 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1172 2325 2458 2917 3126 1172 Size: 1153 133 459 209 2788 Fragments arranged by size: 2788 1153 459 209 133 TatI w'GTAC_w Cuts at: 785 4257 785 Size: 3472 1270 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 629 661 4259 4291 629 Size: 32 3598 32 1080 Fragments arranged by size: 3598 1080 32 32 Tth111I GACn'n_nGTC Cuts at: 388 2539 388 Size: 2151 2591 XbaI T'CTAG_A Cuts at: 1773 1773 Size: 4742 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1485 2237 1485 Size: 752 3990 XhoI C'TCGA_G Cuts at: 677 677 Size: 4742 XmaI C'CCGG_G Cuts at: 1762 1762 Size: 4742 Enzymes that do cut: AccI Acc65I AclI AcuI AfeI AflIII AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbvCI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PfoI PmeI PpiI PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI Enzymes that do not cut: AarI AatII AflII AgeI AhdI AleI AloI AscI AvrII BaeI BbsI BcgI BfrBI BglI BmtI BsiWI BsmBI BspEI BsrGI BssHII BstEII BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PflMI PmlI PpuMI PshAI PsiI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI XmnI ZraI