Restriction map for pF1KB6564
##################################
# NO CUT ENZYMES (  115 enzymes) #
##################################

AatII       AclI        AcsI        AflII       AflIII      
AgeI        AhdI        Alw44I      AocI        Aor51HI     
ApaLI       ApoI        AscI        AseI        AsnI        
AspEI       AspHI       AspI        AviII       AvrII       
BamHI       BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BfrI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaI        BsiHKAI     BsiWI       BsmAI       BsmBI       
Bsp1407I    BspDI       BspHI       BspLU11I    BsrDI       
BsrGI       BsrI        BssHII      Bst1107I    BstZ17I     
Bsu36I      CelII       ClaI        DraI        DraIII      
DrdI        Eam1105I    EarI        Eco47III    Eco81I      
EcoRI       EcoRV       EcoT22I     FbaI        FspI        
HindIII     HpaI        Ksp632I     KspI        MaeII       
MamI        MfeI        MluI        MseI        MunI        
NdeI        NheI        NotI        NruI        NsiI        
NspI        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        PvuII       RcaI        
SacI        SacII       SapI        SbfI        ScaI        
SfiI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
Tru9I       Tsp509I     Tth111I     Van91I      XhoI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

Acc65I        791  GGTACC               G^GTACC
AccI          850  GTCGAC               GT^[AC][GT]AC
ApaI           60  GGGCCC               GGGCC^C
Asp700I       163  GAAGCCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       791  GGTACC               G^GTACC
BanII          60  GGGCCC               G[GA]GC[CT]^C
BfaI          611  CTAG                 C^TAG
BsiEI         889  CGGCCG               CG[GA][CT]^CG
BsmFI         765  GTCCC                GGGAC(10/14)
BsmI          370  GAATGC               GAATGC(1/-1)
BssSI         601  CTCGTG               CACGAG(-5/-1)
BstAPI        357  GCAGGTCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI         214  TTCGAA               TT^CGAA
BstEII        255  GGTCACC              G^GT[ACGT]ACC
BstPI         255  GGTCACC              G^GT[ACGT]ACC
BstXI         815  CCAAAGAGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CpoI         1111  CGGTCCG              CG^G[AT]CCG
EagI          889  CGGCCG               C^GGCCG
EclXI         889  CGGCCG               C^GGCCG
Eco52I        889  CGGCCG               C^GGCCG
EcoNI         462  CCTGGACCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       255  GGTCACC              G^GT[ACGT]ACC
FseI          838  GGCCGGCC             GGCCGG^CC
HgaI          811  GACGC                GACGC(5/10)
HincII        850  GTCGAC               GT[CT]^[GA]AC
HindII        850  GTCGAC               GT[CT]^[GA]AC
KpnI          791  GGTACC               GGTAC^C
MaeI          611  CTAG                 C^TAG
NspV          214  TTCGAA               TT^CGAA
PleI          661  GACTC                GAGTC(4/5)
PpuMI         359  AGGTCCT              [GA]G^G[AT]CC[CT]
RsrII        1111  CGGTCCG              CG^G[AT]CCG
SalI          850  GTCGAC               G^TCGAC
SfuI          214  TTCGAA               TT^CGAA
SmaI          901  CCCGGG               CCC^GGG
SmlI          313  CTTGAG               C^T[CT][GA]AG
TfiI          492  GATTC                G^A[AT]TC
TspRI         414  CAGTG                CA[GC]TG[ACGT][ACGT]^
XbaI          610  TCTAGA               T^CTAGA
XcmI          671  CCAACTCCTTCGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI         901  CCCGGG               C^CCGGG
XmaI          901  CCCGGG               C^CCGGG
XmnI          163  GAAGCCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AccIII        332  TCCGGA               T^CCGGA
              705  TCCGGA              
AlwNI         358  CAGGTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              469  CAGGTGCTG           
BbeI           82  GGCGCC               GGCGC^C
              905  GGCGCC              
BglI           85  GCCTCCGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              897  GCCCCCCGGGC         
BsaWI         332  TCCGGA               [AT]^CCGG[AT]
              705  TCCGGA              
BseAI         332  TCCGGA               T^CCGGA
              705  TCCGGA              
BseRI         124  GAGGAG               GAGGAG(10/8)
              499  GAGGAG              
BsgI          364  CTGCAC               GTGCAG(16/14)
              736  GTGCAG              
BspEI         332  TCCGGA               T^CCGGA
              705  TCCGGA              
BspMI         107  ACCTGC               ACCTGC(4/8)
              357  GCAGGT              
BstYI         221  GGATCT               [GA]^GATC[CT]
              503  AGATCC              
DdeI          225  CTCAG                C^T[ACGT]AG
              992  CTAAG               
HinfI         492  GATTC                G^A[ACGT]TC
              661  GACTC               
KasI           82  GGCGCC               G^GCGCC
              905  GGCGCC              
MflI          221  GGATCT               [GA]^GATC[CT]
              503  AGATCC              
MroI          332  TCCGGA               T^CCGGA
              705  TCCGGA              
MspA1I        915  CAGCGG               C[AC]G^C[GT]G
             1061  CAGCGG              
NarI           82  GGCGCC               GG^CGCC
              905  GGCGCC              
NcoI          665  CCATGG               C^CATGG
              944  CCATGG              
PstI          109  CTGCAG               CTGCA^G
              355  CTGCAG              
SexAI          98  ACCTGGT              A^CC[AT]GGT
              467  ACCAGGT             
SfoI           82  GGCGCC               GGC^GCC
              905  GGCGCC              
TaqI          215  TCGA                 T^CGA
              851  TCGA                
TthHB8I       215  TCGA                 T^CGA
              851  TCGA                
XhoII         221  GGATCT               [GA]^GATC[CT]
              503  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   75 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AvaI        AvaII       BalI        BanI        
BbvI        BcnI        BmyI        BpmI        BsaHI       
BsaJI       BsiYI       BslI        BsoBI       Bsp1286I    
BsrBI       BsrFI       BssKI       BstF5I      BstNI       
BstUI       Cac8I       CfoI        Cfr10I      Cfr13I      
DpnII       DraII       DsaI        EaeI        EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HhaI        Hin1I       
HinP1I      HpaII       HphI        ItaI        MaeIII      
MboI        MboII       MluNI       MnlI        MscI        
MslI        MspI        MvaI        MvnI        MwoI        
NaeI        NciI        NdeII       NgoMIV      NlaIII      
NlaIV       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        StyI        TseI        Tsp45I      
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