Restriction map for
pF1KB6721
(Circular) (Six-base) MAPSORT of: pF1KB6721.seq Check: 8962 from: 1 to: 3386
pF1KB6721 3386 bp
With 182 enzymes: SgfI *
June 26, 2008 10:48 ..
AccI GT'mk_AC
Cuts at: 424 424
Size: 3386
Acc65I G'GTAC_C
Cuts at: 402 402
Size: 3386
AclI AA'CG_TT
Cuts at: 3226 3226
Size: 3386
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 395 1211 1643 2390 2658 395
Size: 816 432 747 268 1123
Fragments arranged by size:
1123 816 747 432 268
AflIII A'CryG_T
Cuts at: 738 1842 2819 738
Size: 1104 977 1305
Fragments arranged by size:
1305 1104 977
AgeI A'CCGG_T
Cuts at: 1774 1774
Size: 3386
AlwNI CAG_nnn'CTG
Cuts at: 144 691 2258 144
Size: 547 1567 1272
Fragments arranged by size:
1567 1272 547
ApaLI G'TGCA_C
Cuts at: 2156 2156
Size: 3386
ApoI r'AATT_y
Cuts at: 182 390 2739 2995 182
Size: 208 2349 256 573
Fragments arranged by size:
2349 573 256 208
AseI AT'TA_AT
Cuts at: 20 20
Size: 3386
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3386
AvaI C'yCGr_G
Cuts at: 406 406
Size: 3386
BamHI G'GATC_C
Cuts at: 411 411
Size: 3386
BanI G'GyrC_C
Cuts at: 402 816 1064 1099 402
Size: 414 248 35 2689
Fragments arranged by size:
2689 414 248 35
BanII G_rGCy'C
Cuts at: 400 1430 400
Size: 1030 2356
BbeI G_GCGC'C
Cuts at: 820 1068 820
Size: 248 3138
BciVI GTATCCnnnnn_n'
Cuts at: 1284 2045 3386 1284
Size: 761 1341 1284
Fragments arranged by size:
1341 1284 761
BclI T'GATC_A
Cuts at: 906 906
Size: 3386
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 3386
BglII A'GATC_T
Cuts at: 901 2938 901
Size: 2037 1349
BlpI GC'TnA_GC
Cuts at: 498 498
Size: 3386
Bme1580I G_kGCm'C
Cuts at: 1011 1104 2160 1011
Size: 93 1056 2237
Fragments arranged by size:
2237 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 598 679 1014 3158 598
Size: 81 335 2144 826
Fragments arranged by size:
2144 826 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1753 1753
Size: 3386
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 380 380
Size: 3386
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 562 1441 1933 2231 562
Size: 879 492 298 1717
Fragments arranged by size:
1717 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3090 3090
Size: 3386
BsaAI yAC'GTr
Cuts at: 739 739
Size: 3386
BsaBI GATnn'nnATC
Cuts at: 6 900 2937 6
Size: 894 2037 455
Fragments arranged by size:
2037 894 455
BsaHI Gr'CG_yC
Cuts at: 817 1065 1767 3263 817
Size: 248 702 1496 940
Fragments arranged by size:
1496 940 702 248
BsaWI w'CCGG_w
Cuts at: 575 1096 1774 2048 2195 2606 575
Size: 521 678 274 147 411 1355
Fragments arranged by size:
1355 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 377 377
Size: 3386
BseYI C'CCAG_C
Cuts at: 811 1569 2146 2642 811
Size: 758 577 496 1555
Fragments arranged by size:
1555 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 928 974 2182 104
Size: 824 46 1208 1308
Fragments arranged by size:
1308 1208 824 46
BsiHKAI G_wGCw'C
Cuts at: 400 1178 1368 2160 400
Size: 778 190 792 1626
Fragments arranged by size:
1626 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 400 1011 1104 1178 1368 1430 2160 400
Size: 611 93 74 190 62 730 1626
Fragments arranged by size:
1626 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2606 2606
Size: 3386
BspHI T'CATG_A
Cuts at: 2562 2562
Size: 3386
BspMI ACCTGCnnnn'nnnn_
Cuts at: 436 650 952 1333 436
Size: 214 302 381 2489
Fragments arranged by size:
2489 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1678 1732 3216 3381 50
Size: 1628 54 1484 165 55
Fragments arranged by size:
1628 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1298 1298
Size: 3386
BsrFI r'CCGG_y
Cuts at: 1384 1565 1774 1384
Size: 181 209 2996
Fragments arranged by size:
2996 209 181
BssSI C'ACGA_G
Cuts at: 1657 2015 1657
Size: 358 3028
BstAPI GCAn_nnn'nTGC
Cuts at: 144 763 144
Size: 619 2767
BstBI TT'CG_AA
Cuts at: 1747 1747
Size: 3386
BstYI r'GATC_y
Cuts at: 1 411 878 901 1236 1482 2494 2580 2592
Size: 410 467 23 335 246 1012 86 12
Cuts at: 2592 2938 1
Size: 346 449
Fragments arranged by size:
1012 467 449 410 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3386
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1336 1517 1680 1336
Size: 181 163 3042
Fragments arranged by size:
3042 181 163
BtsI GCAGTG_nn'
Cuts at: 629 629
Size: 3386
DraI TTT'AAA
Cuts at: 385 385
Size: 3386
DraIII CAC_nnn'GTG
Cuts at: 192 192
Size: 3386
DrdI GACnn_nn'nnGTC
Cuts at: 1092 1950 1092
Size: 858 2528
EaeI y'GGCC_r
Cuts at: 971 1145 1536 1563 2908 971
Size: 174 391 27 1345 1449
Fragments arranged by size:
1449 1345 391 174 27
EagI C'GGCC_G
Cuts at: 971 971
Size: 3386
EarI CTCTTCn'nnn_
Cuts at: 193 230 1409 1619 193
Size: 37 1179 210 1960
Fragments arranged by size:
1960 1179 210 37
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1594 1904 2050 3196 1594
Size: 310 146 1146 1784
Fragments arranged by size:
1784 1146 310 146
EcoICRI GAG'CTC
Cuts at: 398 398
Size: 3386
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 380 395 1211 1643 2390 2658 380
Size: 15 816 432 747 268 1108
Fragments arranged by size:
1108 816 747 432 268 15
EcoO109I rG'GnC_Cy
Cuts at: 370 525 370
Size: 155 3231
EcoRI G'AATT_C
Cuts at: 182 390 182
Size: 208 3178
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1405 1405
Size: 3386
FspI TGC'GCA
Cuts at: 1167 1167
Size: 3386
HaeII r_GCGC'y
Cuts at: 820 1068 2090 3069 820
Size: 248 1022 979 1137
Fragments arranged by size:
1137 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1228 1258 1260 1290 1228
Size: 30 2 30 3324
Fragments arranged by size:
3324 30 30 2
HincII GTy'rAC
Cuts at: 425 425
Size: 3386
KasI G'GCGC_C
Cuts at: 816 1064 816
Size: 248 3138
KpnI G_GTAC'C
Cuts at: 406 406
Size: 3386
MluI A'CGCG_T
Cuts at: 2819 2819
Size: 3386
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2057 2241 2057
Size: 184 3202
MscI TGG'CCA
Cuts at: 1147 1147
Size: 3386
MslI CAynn'nnrTG
Cuts at: 193 1375 1502 193
Size: 1182 127 2077
Fragments arranged by size:
2077 1182 127
MspA1I CmG'CkG
Cuts at: 141 497 811 1171 2184 2429 141
Size: 356 314 360 1013 245 1098
Fragments arranged by size:
1098 1013 360 356 314 245
NaeI GCC'GGC
Cuts at: 1567 1567
Size: 3386
NarI GG'CG_CC
Cuts at: 817 1065 817
Size: 248 3138
NcoI C'CATG_G
Cuts at: 107 107
Size: 3386
NgoMIV G'CCGG_C
Cuts at: 1565 1565
Size: 3386
NspI r_CATG'y
Cuts at: 439 939 1846 439
Size: 500 907 1979
Fragments arranged by size:
1979 907 500
PciI A'CATG_T
Cuts at: 1842 1842
Size: 3386
PfoI T'CCnGG_A
Cuts at: 357 357
Size: 3386
PmeI GTTT'AAAC
Cuts at: 385 385
Size: 3386
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2525 2557 2525
Size: 32 3354
PpuMI rG'GwC_Cy
Cuts at: 370 370
Size: 3386
PstI C_TGCA'G
Cuts at: 433 1118 433
Size: 685 2701
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3386
PvuII CAG'CTG
Cuts at: 141 811 1171 141
Size: 670 360 2356
Fragments arranged by size:
2356 670 360
RsrII CG'GwC_CG
Cuts at: 1581 1581
Size: 3386
SacI G_AGCT'C
Cuts at: 400 400
Size: 3386
SalI G'TCGA_C
Cuts at: 423 423
Size: 3386
SapI GCTCTTCn'nnn_
Cuts at: 1409 1619 1409
Size: 210 3176
SbfI CC_TGCA'GG
Cuts at: 433 433
Size: 3386
ScaI AGT'ACT
Cuts at: 2903 2903
Size: 3386
SfcI C'TryA_G
Cuts at: 33 429 1114 2107 2298 33
Size: 396 685 993 191 1121
Fragments arranged by size:
1121 993 685 396 191
SfoI GGC'GCC
Cuts at: 818 1066 818
Size: 248 3138
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3386
SmaI CCC'GGG
Cuts at: 408 408
Size: 3386
SmlI C'TyrA_G
Cuts at: 541 1456 1948 2210 541
Size: 915 492 262 1717
Fragments arranged by size:
1717 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3386
SphI G_CATG'C
Cuts at: 439 939 439
Size: 500 2886
StyI C'CwwG_G
Cuts at: 107 520 874 107
Size: 413 354 2619
Fragments arranged by size:
2619 413 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 969 1102 1561 1770 969
Size: 133 459 209 2585
Fragments arranged by size:
2585 459 209 133
TatI w'GTAC_w
Cuts at: 2901 2901
Size: 3386
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 362 394 2903 2935 362
Size: 32 2509 32 813
Fragments arranged by size:
2509 813 32 32
Tth111I GACn'n_nGTC
Cuts at: 244 1183 244
Size: 939 2447
XbaI T'CTAG_A
Cuts at: 417 417
Size: 3386
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 881 881
Size: 3386
XmaI C'CCGG_G
Cuts at: 406 406
Size: 3386
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PfoI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BmgBI BmtI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI